检索结果-南通市图书馆

The function of three indigenous plasmids in Mesorhizobium huakuii 2020 and its symbiotic inter-action with Sym pJB5JI of Rhizobium leguminosarum

引用

Science China(Life Sciences) 2008年第4期51卷 353-361页

作者： YANG ChengYun, LI YouGuo, WEI Li, CHENG GuoJun & ZHOU JunChu State Key Laboratory of agricultural Microbiology, huazhong agricultural university, Wuhan 430070, China State Key Laboratory of agricultural Microbiology Huazhong Agricultural University Wuhan China

A Mesorhizobium huakuii strain 2020, isolated from a rice-growing field in southern China, contains three indigenous plasmids named p2020a, p2020b and p2020c, respectively. The plasmids were deleted via Tn5-sacB insertion, and two cured derivatives were obtained. Interestingly, the mutant 2020D29 curing of p2020c could significantly enhance the capacity of symbiotic nitrogen fixation. But the mutant 2020D8 curing of p2020b lost the ability to nodulate Astragalus sinicus. Furthermore, the third plasmid p2020a could be hardly eliminated, suggesting that some house-keeping genes necessary for strain growth located on this plasmid. Then the Sym plasmid pJB5JI of R. leguminosarum bv. viciae was transferred into 2020 and its cured derivatives. The pot plant test showed that the ability of competition and symbiotic nitrogen fixation of transconjugant 2020-137 (pJB5JI) was increased evidently in con-trast to 2020. pJB5JI could not restore the ability of 2020D8 to nodulate Astragalus sinicus. 2020D8-8 (pJB5JI) could form ineffective nodules on peas, which implied that the symbiotic plasmid pJB5JI could express its function at the chromosomal background of Mesorhizobium huakuii 2020. The plas-mid stability was checked in transconjugants under free-living and during symbiosis. The results indi-cated that pJB5JI failed to be detected in some nodule isolates. That Km resistance gene could be am-plified from all transconjugants and nodule isolates suggested that pJB5JI was fully or partially inte-grated into the chromosome of recipients.

关键词： Mesorhizobium huakuii indigenous plasmids pJB5JI symbiotic interaction plasmid curing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cloning and Identification of Porcine SMPX Differentially Expressed in F1 Crossbreds and Their Parents

引用

Acta Biochimica et Biophysica Sinica 2006年第11期38卷 753-758页

作者： Zhu-Qing REN Yuan-Zhu XIONG* Chang-Yan DENG Ming-Gang LEI agricultural Ministry Key Laboratory of Swine Breeding and Genetics,College of Animal Science and Technology,huazhong agricultural university,Wuhan 430070,China agricultural Ministry Key Laboratory of Swine Breeding and Genetics College of Animal Science and Technology Huazhong Agricultural UniversityWuhan 430070 China

In order to investigate porcine heterosis on the molecular basis,Large White (L),a Europeanpurebred,and Meishan (M),a Chinese indigenous purebred,were hybridized directly and reciprocally toproduce F1 hybrids,Large White×Meishan (LM) and Meishan×Large White (ML) *** mRNAdifferential display,we found an expression sequence tag (EST) differentially expressed in F1 hybrids andtheir parents,designated as EST55,which was homologous to human and murine skeletal muscle protein(SMPX),and the full-length cDNA of porcine SMPX was cloned by the rapid amplification of cDNA end(RACE) *** of the mRNA transcript revealed an open reading frame (ORF) of 86 aminoacid residues encoding a nuclear location signal peptide,two overlapping casein kinase Ⅱ phosphorylationsites and one N-glycosylation site with theoretical molecular weight of 9.3 *** analysis revealedthat the deduced protein sequence shared 94%,83% and 78% homology with that of its human,mouse andrat counterparts,*** transcription-polymerase chain reaction (RT-PCR) analysis showedthat it was expressed predominantly in skeletal and heart muscles,whereas at a moderate level in backfat,spleen,stomach and uterus *** single nucleotide polymorphism (SNPs),located in 5′- and 3′-untranslated region (UTR),respectively,were identified by PCR and *** tree and thesecondary structure prediction were also *** possible relationship between porcine SMPX andheterosis was discussed.

关键词： skeletal muscle protein pig mRNA differential display expression pattern

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Reappraisal of phylogenetic status and genetic diversity analysis of Asian population of Lentinula edodes

引用

Progress in Natural Science:Materials International 2006年第3期16卷 274-280页

作者： XU Xuefeng, LI Anzheng, CHENG Shuiming, LIN Fanxue and LIN Fangcan (State Key Laboratory of agricultural Microbiology and Institute of Applied Mycology, huazhong agricultural university, Wuhan 430070, China) State Key Laboratory of agricultural Microbiology and Institute of Applied Mycology Huazhong Agricultural University Wuhan 430070 China

Phylogenetic relationship within the Lentinula genus is constructed based on the sequenced ITS fragments of the 60 Chinese wild L. edodes isolates and the sequence data of 48 isolates of different species from other districts downloaded from the Gen-Bank. The 108 isolates of Lentinula genus are divided into two branches and seven groups, one branch and two groups in the New World, and the other branch and five groups in the Old World, and the isolates clustering of different groups corresponds obviously with the classification of the morphological species. Asian isolates are partitioned in group Ⅰ and Ⅴ, two of the five groups of the Old World, by which the germplasm resources status represented is of great importance shown by the phylogenetic analysis. Group Ⅴ which fills up the blank of geographic distribution has become one of the mainstream groups with an increased isolate number, while group Ⅰ has a tendency to dissimilate into two subgroups (Ia and Ib) with a huge isolate quantity and a coverage of most tested districts, suggesting that China (or Asia) is an important genetic diversity center of the natural population of Lentinula genus. Genetic analysis of Asian isolates based on groups Ia, Ib and group Ⅴ indicates that the diversity of the east coastal-land, northwestern highland and southwestern China and Himalayas districts is the most plentiful, which is the three priorities in diversity protection of Asian Lentinula population.

关键词： Asia Lentinula ITS (internal transcribed spacer) phytogeny genetic diversity.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Isolation,Sequence Analysis and Expression Profile of a Novel Swine Gene Differentially Expressed in the Longissimus Dorsi Muscle Tissues from LandracexLarge White Cross-combination

引用

Acta Biochimica et Biophysica Sinica 2005年第3期37卷 186-191页

作者： Yong-Gang LIU Yuan-Zhu XIONG Chang-Yan DENG Key Laboratory of Swine Genetics and Breeding.Ministry of Agriculture,huazhong agricultural university,Wuhan 430070.China Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture Huazhong Agricultural UniversityWuhan 430070 China

The mRNA differential display technique was performed to investigate the differences in geneexpression in the Longissimus dorsi muscle tissues from LandracexLarge White *** gene that was differentially expressed was identified using semi-quantitative reverse transcriptase-polymerase chain reaction（RT-PCR）and its complete cDNA sequence was obtained using the rapid amplifi-cation of cDNA ends（RACE）*** nucleotide sequence of the gene is not homologous to any of theknown porcine *** sequence prediction analysis revealed that the open reading frame of this geneencodes a protein of 260 amino acids that contains the putative conserved domain of the carbonic anhydrase,and this protein has high homology with the carbonic anhydrase Ⅲ（CA-Ⅲ）of four species—mouse（91%）,horse（91%）,rat（89%）and human（86%）—so that it can be defined as swine carbonic anhydrase Ⅲ.Thephylogenetic tree analysis revealed that the swine CA-Ⅲ has a closer genetic relationship with the horse CA-Ⅲthan with those of mouse,rat and *** tissue expression analysis indicated that the swine CA-Ⅲ geneis generally expressed in most *** experiment is the first to establish the primary foundation forfurther research on the swine CA-Ⅲ gene.

关键词： mRNA differential display rapid amplification of cDNA ends(RACE) novel gene carbonic anhydrase Ⅲ(CA-Ⅲ)

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Adsorption of Cadmium by Soil Colloids and Minerals in Presence of Rhizobia

引用

Pedosphere 2000年第4期10卷 299-307页

作者： HUANGQIAOYUN CHENWENLI 等 DepartmentofResources EnvironmentandAgrochemistryHuazhongAgriculturalUniversityWuhan430070China DepartmentofMicrobiology HuazhongAgriculturalUniversityWuhan430070China

Experiments were conducted to study the adsorption of Cd on two soil colloids (red soil and yellow- brown soil) and three variable-charge minerals (goethite, noncrystalline Fe oxide and kaolin) in the absence and presence of rhizobia. The tested strain Rhizobium fredii C6, tolerant to 0.8 mmol L-1 Cd, was selected from 30 rhizobial strains. Results showed that the isotherms for the adsorption of Cd by examined soil colloids and minerals in the presence of rhizobia could be described by Langmuir equation. Within the range of the numbers of rhizobial cells studied, the amount of Cd adsorbed by each system increased with increasing rhizobial cells. Greater increases for the adsorption of Cd were found in red soil and kaolin systems. Rhizobia influence on the adsorption of Cd by examined soil colloids and minerals was different from that on the adsorption of Cu. The presence of rhizobia increased the adsorption sanity of soil colloids and minerals for Cd, particularly for the goethite and kaolin systems. The discrepancies in the influence of rhizobia on the adsorbability and affinity of selected soil colloids and minerals for Cd suggested the different interactions of rhizobia with various soil components. It is assumed that bacterial biomass plays an important role in controlling the mobility and bioavailability of Cd in soils with kaolinite and goethite as the major colloidal components, such as in variable-charge soil.

关键词： cadmium adsorption iron oxide kaolin rhizobia soil colloid

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Wavelet Transform for Predicting Apoptosis Proteins Subcellular Location

引用

Wuhan university Journal of Natural Sciences 2010年第2期15卷 103-108页

作者： SONG Chaohong, SHI Feng College of Science, huazhong agricultural university, Wuhan430070, Hubei, China College of Science Huazhong Agricultural University Wuhan China

Apoptosis proteins have a central role in the develop- ment and homeostasis of an organism, and their function is related to their types. In this paper, we constructed the character vectors of apoptosis proteins from their amino acid sequences by using the discrete wavelet transform, combined with support vector machine, to predict the type of given apoptosis proteins. For the widely used dataset z98, high success rates were obtained by Jackknife test, and the Matthews correlation coefficients were 0.92, 0.90, 0.81 and 0.80, respectively, which were higher than the other methods on average.

关键词： wavelet transform support vector machine subcellular location

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Isolation, cDNA sequence analysis and tissue expression profile of a novel swine gene differentially expressed in the Longissimus dorsi muscle tissues from Large White x Meishan cross combination

引用

Progress in Natural Science:Materials International 2005年第12期15卷 1073-1078页

作者： LIU Yonggang,LEI Minggang,XIONG Yuanzhu and DENG Changyan Key Laboratory of Swine Genetics and Breeding,Ministry of Agriculture,huazhong agricultural university,Wuhan 430070,China Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture Huazhong Agricultural University Wuhan 430070 China

In order to study the molecular mechanism of heterosis in pigs,the mRNA differential display technique was performed to investigate the differences in gene expression in the Longissimus dorsi muscle tissues from Large White×Meishan cross combination. One novel gene differentially expressed between the hybrids and the purebreds was isolated and subsequently identified using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and its complete cDNA sequence was obtained using the rapid amplification of cDNA ends (RACE) *** nucleotide sequence of the gene is not homologous to any of the known porcine *** sequence prediction revealed that the open reading frame of this gene encodes a protein of 188 amino acids that contains the putative conserved domain of the PRAl family protein and this protein has high homology with the PRA1 family protein 3 of three species-rat (88% ),human (88%),and mouse (87%),-so that it can be defined as swine PRA1 family protein *** phylogenetic tree analysis revealed that the swine PRA1 family protein 3 has a closer genetic relationship with the human PRA1 family protein 3 than with those of mouse and rat. The tissue expression analysis indicated that swine PRA1family protein 3 gene is highly-expressed in muscle and fat,moderately in spleen, weakly in heart,kidney,ovary,lung,and almost not expressed in small intestine and *** function of this gene and the relationship between this gene and heterosis are also discussed.

关键词： pig heterosis Longissimus dorsi mRNA differential display RACE PRA1 family protein 3

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Application of Ground Phosphate Rock to Dimmish the Effects of Simulated Acid Rain on Soil Properties

引用

Pedosphere 1992年第2期2卷 147-152页

作者： DONG YUAN-VAN and LI XUE-YUAN huazhong agricultural university. Wuhan 430070 ( China) huazhongagriculturaluniversity Wuhan430070(China)

The effects of simulated acid rain retained in soil on the properties of acid soil and its diminishing by application of ground phosphate rock were investigated by using the sorption method. Results show as follows: (1) For yellow brown soil, the effect of simulated acid rain on the properties of soil with a pH value of 5.9 was relatively small, except a great quantity of acid rain deposited on it. (2) For red soil, the effect of simulated acid rain on the properties of soil was significant. With the increase of the amount of acid deposition, the pH value of soil was declined, but the contents of exchangeable H+, Al3+ and Mn2+ and the amount of SO42- retention were increased. (3) Many properties of acid soils could be improved by applying ground phosphate rock. For example, pH value of soils and the amounts of available P and exchangeable Ca2+ and Mg2+ were increased, and the amounts of exchangeable H+ and Al3+ and SO42- retained was reduced. The application of ground posphate rock could effctively dimmish the pollution of acid rain to soil.

关键词： phosphate rock simulated acid rain soil properties

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

网络虚拟社区在图书馆人性化服务中的实践应用——以华中农业大学图书馆为例

引用

图书馆工作与研究 2009年第6期 72-73页

作者：谭永钦熊婵李翠霞华中农业大学图书馆

网络虚拟社区与现实社区都包含着一定的场所、一定的人群以及相互提供各种交流信息的特点。图书馆的信息咨询服务要深入到虚拟社区中,为读者搭建信息服务平台,组织读者相互服务。由此,图书馆信息服务的空间将得到有效地拓展。

关键词：虚拟社区人性化服务图书馆

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Wetting Rate and Clay Content Effects on Interrill Erosion in Ultisols of Southeastern China

引用

Pedosphere 2010年第1期20卷 129-136页

作者： YAN Feng-Ling, SHI Zhi-Hua , CAI Chong-Fa and LI Zhao-Xia Key Laboratory of Subtropical Agriculture & Environment, Ministry of Agriculture, huazhong agricultural university, Wuhan 430070 (China) Key Laboratory of Subtropical Agriculture & Environment Ministry of Agriculture Huazhong Agricultural University Wuhan 430070 (China)

An aggregate stability test and a simulated rainfall test were conducted on four representative Ultisols from southeastern China. The soils selected, with clay contents ranging between 117 and 580 g kg-1 , were derived from shale and Quaternary red clay. The stability of aggregates (2–5 mm in diameter) obtained from the soil samples were determined by the Le Bissonnais method. For determination of infiltration, runoff, and erosion, the soil samples were packed in 30 cm × 60 cm trays, wetted at rates of 2, 10, and 60 mm h-1 , and then exposed to simulated rainfall at 60 mm h-1 for 1 h. The results indicated that both aggregate stability and slaking caused by fast wetting increased with increasing clay content. The effect of wetting rate (WR) on infiltration and seal formation varied with clay contents. In the soil with low clay content (sandy loam), the infiltration rate was affected slightly by WR due to low aggregate stability and slaking. In the soils with medium clay content (silt clay loam and clay), WR affected infiltration significantly due to the high aggregate slaking force. In the soil with high clay content, the effect of WR on infiltration was significant, but not as evident as in the soils with medium clay content, which may be related to high aggregate stability by wetting partially compensating for slaking force. The effect of WR on soil loss was similar to that of runoff, but more pronounced. The findings from this study indicated that the relationship between wetting rate and clay content should be considered when predicting interrill erosion in Ultisols.

关键词： aggregate stability infiltration seal formation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：