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p120 Catenin Translocation is Involved in Enhancement of Hepatoma Cellular Malignant Features

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Chinese Journal of Clinical Oncology 2005年第4期2卷 693-699页

作者： huayi huang Chaozan Nong Weisheng He Lingxiao Guo Shaoyun Nong Lili Pan Xiliang Zha Department of Experimental Center Guangxi Nationalities Hospital Nanning 530001 China. Department of Biochemistry and Molecular Biology Fudan University College of Medicine Shanghai 200032 China.

OBJECTIVE To investigate the relationship between p120ctn translocation and hepatocellular carcinoma cell malignant features and the relationship between p120ctn and β-catenin translocation in cell signaling.METHODS Human hepatocellular carcinoma cells were over expressed with p120ctn isoform 3A using a DNA transfection method. The effects of transfection and expression of p120ctn and its binding capacity to E-cadherin were examined using immunoprecipitation and immunoblotting methods. P120ctn subcellular localization and its relation with β-catenin were detected using immunofluorescent microscopy. P120ctn phosphorylation was produced by EGF treatment. Cell adhesion, cell migration and cell proliferation were also examined in this study.RESULTS We found that p120ctnexpression was increased after transfection and the binding capacity of p120ctn to E-cadherin was enhanced. Tyrosine phosphorylation of p120ctn increased after transfection and EGF treatment.p120ctn and β-catenin cellular localization displayd a membrane and cytoplasmic expression pattern, but they translocated into the nucleus for relocalization after p120ctn overexpression plus EGF stimulation. Cell adhesion ability was increased and migration ability reduced after transfection without EGF. Following transfection without EGF cellular proliferation was reduced,but increased after EGF treatment.CONCLUSION Our results suggest that p120ctn plays an important role in hepatocellular carcinoma cell adhesion, migration and proliferation. In addition there is a relationship between p120ctn and β-catenin subcellular localization and signaling.

关键词： p120 肝细胞瘤细胞特征细胞迁移细胞信号

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湛江市古树资源特征分析

桉树科技 2024年第1期41卷 57-63页

作者：谢继红郭乐东赵奕黄华毅广东省林业科学研究院/广东省森林培育与保护利用重点实验室广东广州510520

为摸清湛江市古树资源情况,加强湛江市古树保护工作,基于广东省古树名木普查成果数据,对湛江市古树资源的树种结构、树龄、分布、生长环境、生长状况等方面进行了分析。结果表明:湛江市共有古树7356株,隶属于46科84属111种;在湛江各县... 详细信息

为摸清湛江市古树资源情况,加强湛江市古树保护工作,基于广东省古树名木普查成果数据,对湛江市古树资源的树种结构、树龄、分布、生长环境、生长状况等方面进行了分析。结果表明:湛江市共有古树7356株,隶属于46科84属111种;在湛江各县级市、县区均有分布,其中徐闻县古树数量最多;优势树种主要是榕树(Ficusmicrocarpa)、垂叶榕(F.benjamina)、鹊肾树(Streblusasper)、樟(Camphoraofficinarum)等观赏树种和龙眼(Dimocarpuslongan)、荔枝(Litchi chinensis)等果树;古树生长场所主要为乡村,树龄主要分布在100~200年之间,三级古树多达7080株,占比96.25%;古树树高主要分布范围是7~20 m,平均树高为12.97 m,胸径主要分布范围是40~160 cm,平均胸径96.7 cm,冠幅主要分布范围4~20 m,平均冠幅13.55 m。总体而言,湛江市古树生长环境和生长势良好,古树资源物种多样性丰富。

关键词：湛江市古树树龄胸径分布特征

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我国蚂蚁和白蚁防治农药登记现状与展望

农药科学与管理 2024年第3期45卷 1-6页

作者：肖丽娜高成龙黄华毅扈丽丽黄咏槐陈刘生赵丹阳崔高峰广东省林业科学研究院广东省森林培育与保护利用重点实验室广东广州510520

有些蚂蚁和白蚁种类是重要的世界性害虫,对人类生活造成显著影响,有关防治工作特别是化学防治一直受到密切关注。本文整理了我国蚂蚁和白蚁防治登记用药,汇总分析了蚂蚁、红火蚁、白蚁等化学防治药剂的基础数据,提出了加强农药登记监管... 详细信息

有些蚂蚁和白蚁种类是重要的世界性害虫,对人类生活造成显著影响,有关防治工作特别是化学防治一直受到密切关注。本文整理了我国蚂蚁和白蚁防治登记用药,汇总分析了蚂蚁、红火蚁、白蚁等化学防治药剂的基础数据,提出了加强农药登记监管和市场抽检、鼓励有效成分和配方制剂研发、进行应用场景分类和抗性监测等结论和建议,以期能够在指导药剂合理使用、促进农药产品升级、推动新型农药发展等方面发挥积极作用。

关键词：蚂蚁白蚁红火蚁农药登记现状与展望

Mitigating growth-stress tradeoffs via elevated TOR signaling in rice

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Molecular Plant 2024年第2期17卷 240-257页

作者： Wei Li Jiaqi Liu Zeqi Li Ruiqiang Ye Wenzhen Chen Yuqing huang Yue Yuan Yi Zhang huayi Hu Peng Zheng Zhongming Fang Zeng Tao Shiyong Song Ronghui Pan Jian Zhang Jumim Tu Jen Sheen Hao Du State Key Laboratory of Rice Biology College of Agriculture and BiotechnologyZhejiang UniversityYu-Hang-Tang Road No.866Hangzhou 310058China ZJU-Hangzhou Global Scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311215China Hainan Institute of Zhejiang University Sanya 572025China National Key Laboratory of Plant Molecular Genetics CASCenter for Excellence in Molecular Plant SciencesShanghai lnstitute of Plant Physiology and EcologyChineseAcademy of SciencesShanghai 200032China Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) College of Agricultural SciencesGuizhouUniversityGuiyang 550025China State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 311400China Department of Molecular Biology and Center for Computational and Integrative Biology Massachusetts General Hospitaland Department of GeneticsHarvard Medical SchoolBostonMA02114USA

Rice production accounts for approximately half of the freshwater resources utilized in agriculture,result-ing in greenhouse gas emissions such as methane(CH4)from flooded paddy fields.To address this chal-lenge,environmentally friendly and cost-effective water-saving techniques have become widely adopted in rice cultivation.However,the implementation of water-saving treatments(WsTs)in paddy-field rice has been associated with a substantial yield loss of up to 50%as well as a reduction in nitrogen use efficiency(NUE).In this study,we discovered that the target of rapamycin(TOR)signaling pathway is compromised in rice under WsT.Polysome profiling-coupled transcriptome sequencing(polysome-seq)analysis unveiled a substantial reduction in global translation in response to WST associated with the downregulation of TOR activity.Molecular,biochemical,and genetic analyses revealed new insights into the impact of the positive TOR-S6K-RPS6 and negative TOR-MAF1 modules on translation repression under WST.Intriguingly,ammonium exhibited a greater ability to alleviate growth constraints under WsT by enhancing TOR signaling,which simultaneously promoted uptake and utilization of ammonium and nitrogen allocation.We further demonstrated that TOR modulates the ammonium transporter AMT1;1 as well as the amino acid permease APP1 and dipeptide transporter NPF7.3 at the translational level through the 5'untranslated region.Collectively,these findings reveal that enhancing TOR signaling could mitigate rice yield penalty due to WST by regulating the processes involved in protein synthesis and NUE.Our study will contribute to the breeding of new rice varieties with increased water and fertilizer utilization efficiency.

关键词： target of rapamycin TOR water-saving rice low-carbon agriculture drought nitrogen use efficiency NUE

Associations of daily sedentary behavior,physical activity,and sleep with irritable bowel syndrome:A prospective analysis of 362,193 participants

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Journal of Sport and Health Science 2024年第1期13卷 72-80页

作者： Xu Gao Sifan Tian Ninghao huang Gang Sun Tao huang Department of Occupational and Environmental Health Sciences School of Public HealthPeking UniversityBeijing 100191China Department of Epidemiology and Biostatistics School of Public HealthPeking UniversityBeijing 100191China Department of Gastroenterology and Hepatology First Medical Center of People's Liberation Army General HospitalBeijing 100853China

Background:Irritable bowel syndrome(IBS)substantially affects quality of life and requires early prevention.This study aimed to elucidate the relationships between IBS and daily behaviors,including sedentary behavior(SB),physical activity(PA),and sleep.In particular,it seeks to identify healthy behaviors to reduce IBS risk,which previous studies have rarely addressed.Methods:Daily behaviors were retrieved from self-reported data of 362,193 eligible UK Biobank participants.Incident cases were determined by self-report or health care data according to RomeⅣcriteria.Results:A total of 345,388 participants were IBS-free at baseline,during a median follow-up of 8.45 years,19,885 incident IBS cases were recorded.When examined individually,SB and shorter(≤7 h/day)or longer(>7 h/day)sleep duration were each positively associated with increased IBS risk,and PA was associated with lower IBS risk.The isotemporal substitution model suggested that replacing SB with other activities could provide further protective effects against IBS risk.Among people sleeping≤7 h/day,replacing 1 h of SB with equivalent light PA,vigorous PA,or sleep was associated with 8.1%(95%confidence interval(95%CI):0.901-0.937),5.8%(95%CI:0.896-0.991),and 9.2%(95%CI:0.885-0.932)reduced IBS risk,respectively.For people sleeping>7 h/day,light and vigorous PA were associated with a 4.8%(95%CI:0.926-0.978)and a 12.0%(95%CI:0.815-0.949)lower IBS risk,respectively.These benefits were mostly independent of genetic risk for IBS.Conclusion:SB and unhealthy sleep duration are risk factors for IBS.A promising way to mitigate IBS risk for individuals sleeping≤7 h/day and for those sleeping>7 h/day appears to be by replacing SB with adequate sleep or vigorous PA,respectively,regardless of the genetic predisposition of IBS.

关键词： Irritable bowel syndrome Isotemporal substitution model Physical activity Sedentary behavior Sleep

Fast Blood Oxygenation through Hemocompatible Asymmetric Polymer of Intrinsic Microporosity Membranes

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Research 2024年第1期2023卷 261-270页

作者： Xinxi huang Junping huang Pengcheng Su Wanbin Li Guangdong Key Laboratory of Environmental Pollution and Health School of EnvironmentJinan UniversityGuangzhou 511443China

Membrane technology has attracted considerable attention for chemical and medical applications,among others.Artificial organs play important roles in medical science.A membrane oxygenator,also known as artificial lung,can replenish O_(2) and remove CO_(2) of blood to maintain the metabolism of patients with cardiopulmonary failure.However,the membrane,a key component,is subjected to inferior gas transport property,leakage propensity,and insufficient hemocompatibility.In this study,we report efficient blood oxygenation by using an asymmetric nanoporous membrane that is fabricated using the classic nonsolvent-induced phase separation method for polymer of intrinsic microporosity-1.The intrinsic superhydrophobic nanopores and asymmetric configuration endow the membrane with water impermeability and gas ultrapermeability,up to 3,500 and 1,100 gas permeation units for CO_(2) and O_(2),respectively.Moreover,the rational hydrophobic–hydrophilic nature,electronegativity,and smoothness of the surface enable the substantially restricted protein adsorption,platelet adhesion and activation,hemolysis,and thrombosis for the membrane.Importantly,during blood oxygenation,the asymmetric nanoporous membrane shows no thrombus formation and plasma leakage and exhibits fast O_(2) and CO_(2) transport processes with exchange rates of 20 to 60 and 100 to 350 ml m^(−2) min^(−1),respectively,which are 2 to 6 times higher than those of conventional membranes.The concepts reported here offer an alternative route to fabricate high-performance membranes and expand the possibilities of nanoporous materials for membrane-based artificial organs.

关键词： porosity asymmetric Oxygen

A thermodynamic perspective on electrode poisoning in solid oxide fuel cells

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International Journal of Minerals,Metallurgy and Materials 2024年第6期31卷 1449-1455页

作者： Kevin huang Department of Mechanical Engineering University of South CarolinaColumbiaUSA

A critical challenge to the commercialization of clean and high-efficiency solid oxide fuel cell(SOFC)technology is the insuf-ficient stack lifespan caused by a variety of degradation mechanisms,which are associated with cell components and chemical feedstocks.Cell components related degradation refers to thermal/chemical/electrochemical deterioration of cell materials under operating conditions,whereas the latter regards impurities in feedstocks of oxidant(air)and reductant(fuel).This article provides a thermodynamic perspective on the understanding of the impurities-induced degradation mechanisms in SOFCs.The discussion focuses on using thermodynamic ana-lysis to elucidate poisoning mechanisms in cathodes by impurity species such as Cr,CO_(2),H_(2)O,and SO_(2) and in the anode by species such as S(or H_(2)S),SiO_(2),and P_(2)(or PH_(3)).The author hopes the presented fundamental insights can provide a theoretical foundation for search-ing for better technical solutions to address the critical degradation challenges.

关键词： impurities air fuel reaction activity

In situ direct reprogramming of astrocytes to neurons via polypyrimidine tract-binding protein 1 knockdown in a mouse model of ischemic stroke

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Neural Regeneration Research 2024年第10期19卷 2240-2248页

作者： Meng Yuan Yao Tang Tianwen huang Lining Ke En huang Key Laboratory of Brain Aging and Neurodegenerative Diseases of Fujian Province Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Human Anatomy School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Scientific Research Center School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurology Fujian Medical University Union HospitalFuzhouFujian ProvinceChina Fujian Key Laboratory of Vascular Aging Fujian Medical UniversityFuzhouFujian ProvinceChina

In situ direct reprogramming technology can directly convert endogenous glial cells into functional neurons in vivo for central nervous system repair. Polypyrimidine tract-binding protein 1(PTB) knockdown has been shown to reprogram astrocytes to functional neurons in situ. In this study, we used AAV-PHP.e B-GFAP-sh PTB to knockdown PTB in a mouse model of ischemic stroke induced by endothelin-1, and investigated the effects of GFAP-sh PTB-mediated direct reprogramming to neurons. Our results showed that in the mouse model of ischemic stroke, PTB knockdown effectively reprogrammed GFAP-positive cells to neurons in ischemic foci, restored neural tissue structure, reduced inflammatory response, and improved behavioral function. These findings validate the effectiveness of in situ transdifferentiation of astrocytes, and suggest that the approach may be a promising strategy for stroke treatment.

关键词： astrocyte in situ direct reprogramming ischemic stroke miR-30 based shRNA neuron polypyrimidine tract-binding protein 1 transdifferentiation

Recurrent malignant hyperthermia after scoliosis correction surgery

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World Journal of Emergency Medicine 2024年第1期15卷 70-72页

作者： Junfeng Su Man huang Department of General Intensive Care Unit the Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310052China Key Laboratory of Early Warning and Intervention of Multiple Organ Failure Ministry of Education of the People's Republic of ChinaHangzhou 310052China

Malignant hyperthermia(MH)is a genetic disorder of skeletal muscle cells that affects muscle cytoplasmic calcium homeostasis,with high mortality and low morbidity.Generally,it presents with non-specific signs of a hypermetabolic response,including high fever,tachycardia,and elevated end-tidal carbon dioxide(ETCO_(2)).The successful treatment lies in the timely recognition and early use of dantrolene.[1]As an inhibitor of Ca2+release through ryanodine receptor(RYR)channels,the skeletal muscle relaxant dantrolene has proven to be both a valuable experimental probe of intracellular Ca2+signaling and a lifesaving treatment for MH.[2]Dominant mutations in the skeletal muscle RYR1 gene are well-recognized causes of both malignant hyperthermia susceptibility(MHS)and central core disease(CCD).

关键词： hyperthermia malignant elevated