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Science China(Life Sciences) 2021年第9期64卷 1522-1532页

作者： Xiao-Feng Zhang Tianbao Zeng Yunjie Xie Yuemin Zheng huanqin wang Hanbin Lin Zongwen wang Taiyun Wei State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Institute of Plant VirologyFujian Agriculture and Forestry UniversityFuzhou 350002China

Intracellular polyamines(putrescine,spermidine,and spermine)have emerged as important molecules for viral infection;however,how viruses activate polyamines biosynthesis to promote viral infection remains *** decarboxylase 1(ODC1)and its antienzyme 1(OAZ1)are major regulators of polyamine biosynthesis in animal ***,we report that rice yellow stunt virus(RYSV),a plant rhabdovirus,could activate putrescine biosynthesis in leafhoppers to promote viral propagation by inhibiting OAZ1 *** observed that the reduction of putrescine biosynthesis by treatment with difluormethylornithine(DFMO),a specific nontoxic inhibitor of ODC1,or with in vitro synthesized dsRNAs targeting ODC1 mRNA could inhibit viral *** contrast,the supplement of putrescine or the increase of putrescine biosynthesis by treatment with ds RNAs targeting OAZ1 mRNA could facilitate viral *** further determined that both RYSV matrix protein M and ODC1 directly bind to the ODC-binding domain at the C-terminus of ***,viral propagation in leafhoppers would decrease the ability of OAZ1 to target and mediate the degradation of ODC1,which finally activates putrescine production to benefit viral *** work reveals that polyamine-metabolizing enzymes are directly exploited by a vector-borne virus to increase polyamine production,thereby facilitating viral infection in insect vectors.

关键词： rice yellow stunt virus leafhopper polyamines viral infection plant virus

特发性肺纤维化和炎症性肠病共同核心基因特征及其生物学机制的鉴定

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河南医学研究 2024年第5期33卷 772-779页

作者：娄娇娇王浩冉黄凤祥王焕勤康燕王起龙马开乔瑞萍苗丽君郑州大学第一附属医院呼吸与危重症医学科河南郑州450052

目的基于生物信息学研究介导特发性肺纤维化(IPF)和炎症性肠病(IBD)共存的潜在关键分子和通路。方法通过美国国家生物技术信息中心(NCBI)基因表达综合数据库(GEO)下载IPF(GSE110147)和IBD(GSE752141)数据集。使用R的“limma”包分析差... 详细信息

目的基于生物信息学研究介导特发性肺纤维化(IPF)和炎症性肠病(IBD)共存的潜在关键分子和通路。方法通过美国国家生物技术信息中心(NCBI)基因表达综合数据库(GEO)下载IPF(GSE110147)和IBD(GSE752141)数据集。使用R的“limma”包分析差异表达基因(DEGs)。对DEGs进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)分析。利用STRING数据库和Cytoscape软件进行蛋白质-蛋白质相互作用(PPI)网络构建、模块构建和核心基因鉴定。在IPF的GSE53845和IBD的GSE59071数据中对核心基因进行验证。基于NetworkAnalyst Web平台,构建转录因子(TFs)-核心基因与TFs-微小核糖核酸(miRNAs)的相互作用网络。结果筛选出67个上调基因和23个下调基因进行后续分析。利用CytoHubba和MCODE插件鉴定出6个重要的枢纽基因,包括SPP1、COL1A1、POSTN、MMP7、COL3A1和COL6A3。在TFs基因相互作用网络中,SPP1与其他TFs的相互作用率最高。HINFP、POU2F2、YY1、FOXL1和FOXC1协同参与SPP1的调控。另一方面,在TFs-miRNAs共调控网络中的miRNAs,hsa-miR-301b、hsa-miR301a、hsa-miR-29c、hsa-miR-29b和hsa-miR-29a在3个地方表现出最高的程度,并且都靶向COL6A3。结论这项生物信息学研究揭示了IPF和IBD的共同发病机制,表明IPF和IBD是相似的,这可以为进一步的研究提供新的思路。

关键词：特发性肺纤维化炎症性肠病核心基因转录因子微小核糖核酸

Sugarcane leaves-derived polyphenols alleviate metabolic syndrome and modulate gut microbiota of ob/ob mice

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Food Science and Human Wellness 2024年第2期13卷 633-648页

作者： Li Sun Tao wang Baosong Chen Cui Guo Shanshan Qiao Jinghan Lin Huan Liao huanqin Dai Bin wang Jingzu Sun Hongwei Liu State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Savaid Medicine School University of Chinese Academy of SciencesBeijing 100101China Guangxi Academy of Sciences Nanning 530007China

Sugarcane leaves-derived polyphenols(SLP)have been demonstrated to have diverse health-promoting benefits,but the mechanism of action has not been fully *** study aimed to investigate the anti-metabolic disease effects of SLP and the underlying mechanisms in *** the current study,we prepared the SLP mainly consisting of three flavonoid glycosides,three phenol derivatives,and two lignans including one new compound,and further demonstrated that SLP reduced body weight gain and fat accumulation,improved glucose and lipid metabolism disorders,ameliorated hepatic steatosis,and regulated short-chain fatty acids(SCFAs)production and secondary bile acids metabolism in ob/ob ***,SLP largely altered the gut microbiota composition,especially enriching the commensal bacteria Akkermansia muciniphila and Bacteroides *** gavage with the above two strains ameliorated metabolic syndrome(MetS),regulated secondary bile acid metabolism,and increased the production of SCFAs in high-fat diet(HFD)-induced obese *** results demonstrated that SLP could be used as a prebiotic to attenuate MetS via regulating gut microbiota composition and further activating the secondary bile acids-mediated gut-adipose axis.

关键词： Sugarcane leaves-derived polyphenols Metabolic syndrome Bacteroides acidifaciens Akkermansia muciniphila Secondary bile acids metabolism

Circulating cell-free mtDNA as a new biomarker for cancer detection and management

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Cancer Biology & Medicine 2024年第2期21卷 105-110页

作者： Fan Peng Siyuan wang Zehui Feng Kaixiang Zhou huanqin Zhang Xu Guo Jinliang Xing Yang Liu State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and Xijing Hospital of Digestive Diseases Fourth Military Medical UniversityXi’an 710032China Department of Clinical Diagnosis Tangdu HospitalFourth Military Medical UniversityXi’an 710038China

Cancer is a major cause of death worldwide,and was responsible for 19.29 million new cases and 9.96 million deaths in 2020 *** total number of patients with cancer worldwide is estimated to reach 28 million by *** Ame... 详细信息

关键词： Cancer cancer death

Review on recent progress in on-line monitoring technology for atmospheric pollution source emissions in China

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Journal of Environmental Sciences 2023年第1期123卷 367-386页

作者： huanqin wang Jitong Zhou Xue Li Qiang Ling Hongyuan Wei Lei Gao Ying He Ming Zhu Xiao Xiao Youjiang Liu Shan Li Chilai Chen Guotao Duan Zhimin Peng Peili Zhou Yufeng Duan Jianbing wang Tongzhu Yu Yixin Yang Jiguang wang Zhen Zhou Huaqiao Gui Yanjun Ding State Key Laboratory of Transducer Technology Institute of Intelligent MachinesHefei Institutes of Physical ScienceChinese Academy of SciencesHefei 230031China Institute of Mass Spectrometry and Atmospheric Environment Jinan UniversityGuangzhou 510632China Department of Automation University of Science and Technology of ChinaHefei 230027China China Automotive Technology and Research Center Tianjin 300300China Institute of Solid State Physics Chinese Academy of SciencesHefei 230031China Key Laboratory of Environmental Optics and Technology Anhui Institute of Optics and Fine MechanicsChinese Academy of SciencesHefei 230031China School of Electronic Information and Communications Huazhong University of Science and TechnologyWuhan 430074China School of Optical and Electronic Information Huazhong University of Science and TechnologyWuhan 430074China State Key Laboratory of Power Systems Department of Energy and Power EngineeringTsinghua UniversityBeijing 100084China Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education School of Energy and EnvironmentSoutheast UniversityNanjing 210096China School of Chemical and Environmental Engineering China University of Mining and Technology(Beijing)Beijing 100083China CAS Center for Excellence in Regional Atmospheric Environment Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China

Emissions from mobile sources and stationary sources contribute to atmospheric pollution in China,and its components,which include ultrafine particles(UFPs),volatile organic compounds(VOCs),and other reactive gases,such as NH3and NOx,are the most harmful to human *** has released various regulations and standards to address pollution from mobile and stationary ***,it is urgent to develop online monitoring technology for atmospheric pollution source *** study provides an overview of the main progress in mobile and stationary source monitoring technology in China and describes the comprehensive application of some typical instruments in vital areas in recent *** instruments have been applied to monitor emissions from motor vehicles,ships,airports,the chemical industry,and electric power *** only has the level of atmospheric environment monitoring technology and equipment been improving,but relevant regulations and standards have also been constantly ***,the developed instruments can provide scientific assistance for the successful implementation of *** to the potential problem areas in atmospheric pollution in China,some research hotspots and future trends of atmospheric online monitoring technology are ***,more advanced atmospheric online monitoring technology will contribute to a comprehensive understanding of atmospheric pollution and improve environmental monitoring capacity.

关键词： Atmospheric pollution Mobile sources Stationary sources On-line monitoring technology

Cr12钢电脉冲球化退火热处理工艺参数的优化

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热加工工艺 2008年第14期37卷 72-73,79页

作者：王焕琴宋林森王占合河南科技学院机电学院河南新乡453003

采用正交实验法对Cr12钢进行脉冲球化退火研究,找出了Cr12钢在脉冲电场作用下理想的球化退火工艺参数,并进行了相应的新工艺实验,得到Cr12钢较理想的球化组织,同时降低了Cr12钢的退火加热温度,缩短了保温时间,其硬度可比常规等温球化退... 详细信息

采用正交实验法对Cr12钢进行脉冲球化退火研究,找出了Cr12钢在脉冲电场作用下理想的球化退火工艺参数,并进行了相应的新工艺实验,得到Cr12钢较理想的球化组织,同时降低了Cr12钢的退火加热温度,缩短了保温时间,其硬度可比常规等温球化退火低60HBS左右,更好地改善了Cr12钢的切削加工性能。

关键词：脉冲电场球状珠光体冷模具钢

Survivin基因在牛蒡子苷元增强人肺癌H460细胞化疗敏感性中的作用

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国际呼吸杂志 2015年第6期35卷 401-405页

作者：王焕勤王静郑州大学第一附属医院呼吸内科 450052

目的研究Survivin基因在牛蒡子苷元促进肺癌细胞化疗敏感性中的作用。方法应用RT-PCR技术从H460细胞中扩增全长人SurvivincDNA，并将其克隆至pcDNA3．1（＋）表达载体，构建人Survivin基因真核表达质粒。将pcDNA3．1-Survivin重组质粒... 详细信息

目的研究Survivin基因在牛蒡子苷元促进肺癌细胞化疗敏感性中的作用。方法应用RT-PCR技术从H460细胞中扩增全长人SurvivincDNA，并将其克隆至pcDNA3．1（＋）表达载体，构建人Survivin基因真核表达质粒。将pcDNA3．1-Survivin重组质粒或空载体分别转染H460细胞，孵育24h后，应用Western blot法检测Survivin过表达情况；转染细胞与牛蒡子苷元单独或联合顺铂处理，应用Annexin-V／PI染色法检测细胞凋亡情况。结果与未转染和转染空载体质粒的H460细胞相比，转染pcDNA3．1-Survivin重组质粒H460细胞Survivin水平显著增加，提高大约10倍。低剂量顺铂对H460细胞Survivin蛋白表达影响不显著，而牛蒡子苷元可显著抑制Survivin蛋白表达水平。当牛蒡子苷元与顺铂联合作用时，Survivin蛋白表达抑制程度更大。与转染空载体质粒相比，转染Survivin表达重组质粒的细胞对牛蒡子苷元单独或联合顺铂诱导的凋亡具有抗性，细胞凋亡比率显著下降。结论牛蒡子苷元抑制人肺癌细胞中Survivin基因表达。牛蒡子苷元促人肺癌细胞顺铂敏感性与抑制Survivin通路有关。

关键词：牛蒡子苷元凋亡化疗敏感性顺铂肺癌

慢性阻塞性肺疾病患者长期预后与体质指数关系的研究

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中华老年医学杂志 2014年第1期33卷 35-38页

作者：苗丽君张瑞霞王静王焕勤郑州大学第一附属医院呼吸内科 450052

目的评价慢性阻塞性肺疾病（COPD）住院患者长期生存率和体质指数（BMI）之间的关系。方法采用回顾性队列研究的方法，选取自2007年1月1日至2011年12月31日人住我院的1528例COPD患者中资料完整的1124例为研究对象，患者死亡时间从死亡... 详细信息

目的评价慢性阻塞性肺疾病（COPD）住院患者长期生存率和体质指数（BMI）之间的关系。方法采用回顾性队列研究的方法，选取自2007年1月1日至2011年12月31日人住我院的1528例COPD患者中资料完整的1124例为研究对象，患者死亡时间从死亡登记处和民政部门获得，从病历资料中收集患者的临床特点。1124例患者的BMI中位数为22．7kg／m2，根据BMI的四分位数将患者分为：BMI〈18．9kg／m2组，BMI18．9～22．7kg／m2组，BMI22．8～26．2kg／m2组，BMI〉26．2kg／m2组，采用log—rank检验比较不同组患者的生存率曲线差异，应用Cox比例风险回归模型评价全死因病死率与BMI等因素的关系。结果1124例患者中270例（24．0％）BMI％18．5kg／m2，随访期间共有162例（14．4％）患者死亡。不同BMI四分位数患者的生存率曲线差异具有统计学意义（x^2=7．97，P=0．043），其中BMI处于22．8～26．2kg／m2患者的生存率最高，而BMI〈18．9kg／m2患者的生存率最低。经Cox多因素回归分析调整其他因素影响后，BMI仍为预测死亡的独立危险因素，与BMI〈18．9kg／m2的患者比较，BMI为22．8～26．2kg／m2和BMI〉26．2kg／m2的患者病死率分别降低了41％和35％。结论COPD患者常伴有体质量降低，BMI是预测长期生存率的独立危险因素，且BMI具有容易获得、相对稳定的优点，尤其适合于评估急性加重期COPD患者的预后。

关键词：肺疾病,慢性阻塞性体质量指数

T10A钢在脉冲电场作用下球化退火工艺参数的优化

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热加工工艺 2010年第10期39卷 172-173,176页

作者：魏增菊卢澎涛王焕琴新乡学院机电工程学院河南新乡453003 河南科技学院机电学院河南新乡453003

采用正交实验法,找出了T10A钢在脉冲电场作用下理想的球化退火工艺参数,并进行了相应的新工艺实验。结果得到T10A钢较理想的球化组织,同时降低了T10A钢的退火加热温度,缩短了保温时间,其硬度可比普通退火钢的低50～100 HBS,更好地改善了... 详细信息

采用正交实验法,找出了T10A钢在脉冲电场作用下理想的球化退火工艺参数,并进行了相应的新工艺实验。结果得到T10A钢较理想的球化组织,同时降低了T10A钢的退火加热温度,缩短了保温时间,其硬度可比普通退火钢的低50～100 HBS,更好地改善了T10A钢的切削加工性能。

关键词：电脉冲球化退火 T10A钢

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高场不对称波形离子迁移谱关键测控模块研究

仪器仪表学报 2012年第9期33卷 1974-1979页

作者：刘友江王焕钦陈然孔德义陈池来 Juergen Brugger 中国科学技术大学自动化系中国科学院合肥智能机械研究所传感技术联合国家重点实验室中国科学技术大学物理系瑞士洛桑联邦理工学院

研究了高场不对称波形离子迁移谱(high-field asymmetric waveform ion mobility spectrometer,FAIMS)两个关键测控模块:波形不对称高压产生模块和微弱电流检测模块的设计。通过采用反激升压原理,设计了一种电压幅度在线可调的波形不对... 详细信息

研究了高场不对称波形离子迁移谱(high-field asymmetric waveform ion mobility spectrometer,FAIMS)两个关键测控模块:波形不对称高压产生模块和微弱电流检测模块的设计。通过采用反激升压原理,设计了一种电压幅度在线可调的波形不对称高压产生电路模块,其输出电压峰峰值达1 800 V,频率达1 MHz;微弱电流检测采用超高电阻反馈式I-V转换电路,并对失调电压进行补偿,同时结合PCB割板处理及包裹屏蔽等抗干扰措施,使得微弱电流模块的检测下限优于0.1 pA。最后,通过对比实验研究了不同波形不对称高压与分离效果关系,并以苯为样品对系统的检测下限进行了研究,结果表明采用上述测控模块的FAIMS检测下限达1×10-9。

关键词：高场不对称波形离子迁移谱弱电流检测高压高频不对称波形