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Development of Alfalfa (Medicago sativa L.) Regeneration System and Agrobacterium-Mediated Genetic Transformation

Agricultural Sciences in China 2010年第2期9卷 170-178页

作者： ZHANG He huang Qi-man su Jin Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China

The regeneration ability of four alfalfa （Medicago sativa L.） cultivars, Xinjiang Daye, Longdong, Gannong 1 and Gannong 3, was studied, and the effects of various cultivars, explant sources and medium recipes on regeneration were compared. The better callus forming frequency obtained from hypocotyls of Xinjiang Daye is 88.5% and regeneration frequency is 9.8% in our initial experiments. To further optimize regeneration system for genetic transformation, we therefore changed concentrations of plant growth regulators and supplemented with glutamine into callus-induction and shoot-regeneration media. Callus forming frequency and shoot differentiation frequency were increased to 100%. The time taken to generate transgenic plants （16 weeks） was shorter than that for previouse procedure （25 weeks） and regeneration frequency was promoted to 15.1%. The results show that addition of glutamine is particularly important for shortening period of regeneration and promoting regeneration frequency. For study of genetic transformation of alfalfa, Agrobacterium tumefaciens-mediated transformation of Xinjiang Daye was developed based on this optimized regeneration system. The plant expression vector carrying two glutamine synthetases （GS 1 and GS2） and △1-pyrroline-5-carboxylate synthetase （P5CS） gene was used for alfalfa in vitro transformation. Six transgenic alfalfa plantlets with resistance to PPT were obtained. The introduction of foreign genes into plants was assessed in the transformants by PCR analysis and Southern hybridizations.

关键词： alfalf glutamine synthetases △1-pyrroline-5-carboxylate synthetase Agrobacterium tumefaciens-mediated transformation transgenic alfalfa

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The Relationship between Pendant Phosphate Groups and Mechanical Properties of Polyisoprene Rubber

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Chinese Journal of Polymer Science 2021年第4期39卷 465-473,I0006页

作者： Shi-Qi Li Mao-Zhu Tang Cheng huang Rong Zhang Guang-su huang Yun-Xiang Xu College of Polymer Science&Engineering State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu 610065China

It is still a great challenge to mimic the structure and function of natural rubber by introducing polar components into synthetic *** order to explore the function of phosphate groups on the mechanical properties of polyisoprene rubber,a terminally functionalized compound(PIP-P)containing phosphate groups was synthesized and further vulcanized to prepare the model compound *** analyzing the test results,it was found that these phosphate groups formed polar aggregates in non-polar polyisoprene rubber matrix,serving as an additional dynamic cross-linking sites,which increases the cross-linking density and improves mechanical *** influence of the phosphate groups on the strain-induced crystallization(SIC)was further investigated via synchrotron wide-angle X-ray diffraction(WAXD)*** phosphate group aggregates not only reduced the onset strain of SIC,but also slowed down the molecular chain mobility,which hinder the crystal lateral *** above results help us to gain a deeper understanding for the function of phosphate groups in the formation of"naturally occurring network"and guide the molecular design of next generation polyisoprene rubber.

关键词： Phosphate groups Strain-induced crystallization Naturally occurring network Natural rubber

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Inhomogeneous Natural Network Promoting Strain-induced Crystallization:A Mesoscale Model of Natural Rubber

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Chinese Journal of Polymer Science 2019年第11期37卷 1142-1151页

作者： Han Liu Guang-su huang Lai-Yun Wei Jian Zeng Xuan Fu Cheng huang Jin-Rong Wu College of Polymer Science and Engineering State Key Laboratory of Polymer Materials Engineering Sichuan University

Although synthetic rubbers show continuously improved mechanical properties,natural rubber (NR) remains irreplaceable in the rubber family due to its superior mechanical properties.A mainstream viewpoint regarding the superiority of NR is that NR possesses a natural network formed by linking the poly(cis-l,4-isoprene) chain terminals to protein and phospholipid aggregates;after vulcanization,the natural network additionally contributes to rubber mechanics by both increasing the network density and promoting the strain-induced crystallization (SIC) ***,the reason why the natural network promotes SIC is still unclear;in particular,only using the increased network density cannot explain our finding that the NR shows smaller onset strain of SIC than Gel (the gel component of NR with higher network density) and even vulcanized ***,we point out that the inhomogeneous chain deformation is the alternative reason why SIC of NR takes place at smaller strain than that of *** specifically,although the natural network is homogenous on the subchain length scale based on the proton double-quantum NMR results,it is essentially inhomogeneous on mesoscale (100 nm),as revealed by the small angle X-ray scattering *** inhomogeneous network also leads to the mesoscale deformation inhomogeneity,as detected by the orientation of stearic acid (SA) probe,thus resulting in the smaller onset strain of SIC of *** on the experimental results,a mesoscale model is proposed to qualitatively describe the crucial roles of inhomogeneous structure and deformation of natural network in NR?s mechanical properties,providing a clue from nature to guide the development of high-performance rubbers with controlled structures at mesoscale.

关键词： Natural Rubber Inhomogeneous natural network Strain-induced crystallization Mesoscale model

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High Technology Letters 2017年第2期23卷 179-184页

作者：李文法 Wang Gongming Li Ke huang su Beijing Key Laboratory of Information Service Engineering Beijing Union University National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of Sciences

The performance of conventional similarity measurement methods is affected seriously by the curse of dimensionality of high-dimensional *** reason is that data difference between sparse and noisy dimensionalities occupies a large proportion of the similarity,leading to the dissimilarities between any results.A similarity measurement method of high-dimensional data based on normalized net lattice subspace is *** data range of each dimension is divided into several intervals,and the components in different dimensions are mapped onto the corresponding *** the component in the same or adjacent interval is used to calculate the *** validate this method,three data types are used,and seven common similarity measurement methods are *** experimental result indicates that the relative difference of the method is increasing with the dimensionality and is approximately two or three orders of magnitude higher than the conventional *** addition,the similarity range of this method in different dimensions is [0,1],which is fit for similarity analysis after dimensionality reduction.

关键词： high-dimensional data the curse of dimensionality similarity normalization subspace NPsim

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The Effect of Branching Structure on the Properties of Entangled or Non-covalently Crosslinked Polyisoprene

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Chinese Journal of Polymer Science 2021年第1期39卷 113-121,I0008页

作者： Mo-Kun Chen Rong Zhang Mao-Zhu Tang Guang-su huang Yun-Xiang Xu College of Polymer Science&Engineering State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu 610065China

The branching structures in natural rubber(NR) were believed to be critical for its superior mechanical properties. However, it is challenging to unravel the branching structure-function relationship of NR due to the complexity of the system. Herein, polyisoprene-(polyisoprene-g-polylactide)(PI-PLA) as model compound containing branching structure was designed and synthesized, which can improve the modulus, strength and viscoelasticity activation energy compared to those of the pristine polyisoprene(PI). The reason is that the branching structure contributes to the entanglement between polyisoprene chains. In order to probe the effect of branching structure on noncovalently crosslinked system, the polyisoprene block of PI-PLA was epoxidized and mixed with Fe3+ ions to introduce coordination bonds. Compared with the linear counterpart, the branching structure obviously enhanced activation energy of coordinated polyisoprenes, remarkably improving the mechanical properies of elastomer.

关键词： Natural rubber Branching structure Noncovalent interaction Polyisoprene

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Simultaneous determination of fluorine and iodine in urine by ion chromatography with electrochemical pretreatment

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Chinese Chemical Letters 2009年第12期20卷 1483-1486页

作者： Kang Kang Hu Wei Xiong huang Yu Hua su Rong Zong Hu Department of Chemistry College of Chemistry and Chemical Engineering and The Key Laboratory of Analytical Sciences of the Ministry of Education Xiamen University Xiamen 361005 China

A new method for the simultaneous determination of fluorine and iodine in urine by ion chromatography （IC） with electrochemical pretreatment has been developed. The pretreatment was performed in a novel electrochemical oxidationneutralization device （EOND）, in which iodide of the sample was oxidized to iodate and the alkaline digestion sample solution was neutralized. Under the optimized conditions, the limits of detection （LOD, S/N = 3） were 2.5 μg/L for fluoride and 20 μg/L for iodate, respectively. The recoveries were in the range of 93-102% for fluoride and 86-98% for iodate.

关键词： Fluorine Iodine Urine Electrochemical pretreatment Ion chromatography

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Mechanisms of Dangua Recipe in Improving Glycolipid Metabolic Disorders Based on Transcriptomics

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Chinese Journal of Integrative Medicine 2022年第2期28卷 130-137页

作者： HENG Xian-pei WANG Zhi-ta LI Liang YANG Liu-qing huang su-ping Departme nt of Endocri no logy People's Hospital Affiliated to Fujian University of Traditional Chinese MedicineFuzhou(350004)China Academy of Integrative Medicine Fujian University of Traditional Chinese MedicineFuzhou(350122)China

Objective:To explore the mechanisms of Dangua Recipe(DGR)in improving glycolipid metabolism based on ***:Sprague-Dawley rats with normal glucose level were divided into 3 groups according to a random number table,including a conventional diet group(Group A),a DGR group(Group B,high-calorie diet+20.5 g DGR),and a high-calorie fodder model group(Group C).After 12 weeks of intervention,the liver tissue of rats was *** sequence and transcriptional analysis were performed to identify the key genes related to glycolipid metabolism reflecting DGR efficacy,and then gene or protein validation of liver tissue were *** phosphoribosyl transferase(Nampt)and phosphoenolpyruvate carboxykinase(PEPCK)proteins in liver tissues were detected by enzyme linked immunosorbent assay,fatty acid synthase(FASN)protein was detected by Western blot,and fatty acid binding protein 5(FABP5)-mRNA was detected by quantitative real-time polymerase chain ***,the functional verification was performed on the diabetic model rats by Nampt blocker(GEN-617)injected in *** A1c(HbA1c),plasma total cholesterol and triglycerides were ***:Totally,257 differentialdominant genes of Group A *** C and 392 differential-dominant genes of Group B *** C were ***,11 Gene Ontology molecular function terms and 7 Kyoto Encyclopedia of Genes and Genomes enrichment pathways owned by both Group A *** C and Group C *** B were *** liver tissue target validation showed that Nampt,FASN,PEPCK protein and FABP5-mRNA had the same changes consistent with *** in vivo functional tests showed that GEN-617 increased body weight,HbA1c,triglyceride and total cholesterol levels in the diabetic rats(P<0.05 or P<0.01);while all the above-mentioned levels(except triglyceride)were decreased significantly by GEN-617 combined with DGR intervention(P<0.05 or P<0.01).Conclusion:Nampt activation was one of the

关键词： transcriptomics Dangua Recipe glycolipid metabolism Nampt therapeutic target Chinese medicine

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Anti-Insect Activity of the Methanol Extracts of Fern and Gymnosperm

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Agricultural Sciences in China 2010年第2期9卷 249-256页

作者： huang su-qing ZHANG Zhi-xiang LI You-zhi LI Yu-xia XU Han-hong Key Laboratory of Natural Pesticide ＆ Chemical Biology Ministry of Education/South China Agricultural University Guangzhou 510642 P.R. China College of Bio-Safety Science and Technology Hunan Agricultural University Changsha 410128 P.R.China

With environmental and food safety issues becoming one of the greatest concerns of the public, active plant compounds play more and more important roles in pest controlling. Fern to their particular status in plant taxology and co-evolution are of great significance for application as new pesticides due with insects. In this study, methanol extracts were distilled from wild fern and gymnosperm of 54 species of 22 families collected from Shiwandashan Mountain in Guangxi Zhuang Autonomous Region of China. Insecticidal activities of the methanol extracts were assayed on both adult house fly （Musca domestica） and mosquito （Aedes albopictus）. Significant insecticidal activities were exhibited in the methanol extracts of five species, Cupressusfunebris （leaves and stems）, Cycas acuminatissima （roots）, Keteleeria fortunei （leaves and stems）, Onychium japonicum （whole plant）, and Pinus taiwanensis var. Darning shanensis （leaves and stems）. Applications of the extracts of these plants resulted in higher than 50% mortalities in 4th instar larvae of A. albopictus at 24 h after treatment. The methanol extracts from 13 species possessed insecticidal activities against the adult of M. domestica at 48 h after treatment with higher than 90% mortalities. The extensive screening results showed that these fern and gymnosperm were highly potential to be botanical insecticides. The findings provide a feasible and valuable basis for further investigation.

关键词： plant extracts anti-insect activity Musca domestica Aedes albopictus

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super Strong and Tough Elastomers Enabled by Sacrificial Segregated Network

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Chinese Journal of Polymer Science 2021年第3期39卷 377-386,I0008页

作者： Yong Zhu Lai-Yun Wei Xuan Fu Jun-Qi Zhang Ling-Min Kong Guang-su huang Jin-Rong Wi State Key Laboratory of Polymer Materials Engineering College of Polymer Science and EngineeringSichuan UniversityChengdu 610065China

The integration of high strength and toughness concurrently is a vital requirement for elastomers from the perspective of long-term durability and reliability. Unfortunately, these properties are generally conflicting in artificial materials. In the present work, we propose a facile strategy to simultaneously toughen and strengthen elastomers by constructing 3 D segregated filler network via a simple latex mixing *** as-fabricated elastomers are featured by a microscopic 3 D interconnected segregated network of rigid graphene oxide(GO) nanosheets and a continuous soft matrix of sulfur vulcanized natural rubber(NR). We demonstrate that the interconnected segregated filler network ruptures preferentially upon deformation, and thus is more efficient in energy dissipation than the dispersed filler network. Therefore, the segregated filler network exhibits better reinforcing effects for the rubber matrix. Moreover, the excellent energy dissipating ability also contributes to the outstanding crack growth resistance through the release of concentrated stress at the crack tip. As a result, the strength, toughness and fatigue resistance of the nanocomposites are concurrently enhanced. The methodology in this work is facile and universally applicable, which may provide new insights into the design of elastomers with both extraordinary static and dynamic mechanical performance for practical applications.

关键词： Elastomers Nanocomposites Segregated network Sacrificial bonds Mechanical properties

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THERMAL SHOCK OF SEMI-INFINITE BODY WITH MULTI-PULSED INTENSE LASER RADIATION

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Acta Mechanica Solida Sinica 2010年第2期23卷 175-180页

作者： Haiming huang 1 Fang su Yue sun(Institute of Engineering Mechanics,Beijing Jiaotong University,Beijing 100044,China) Institute of Engineering Mechanics Beijing Jiaotong University Beijing China

In order to investigate the thermal shock and the heat conduction property of a target under multi-pulsed laser radiation, analytic expressions of both temperature and thermal stress fields in the target are deduced on the basis of the non-Fourier conduction law and the thermo-elastic theory. Taking a stainless steel target as an example, we can solve the analytic expressions under appropriate boundary conditions by using the finite difference method and MATLAB software, and then reveal the evolution law of both surplus temperaturt, and thermal stress in the target. The results indicate that the temperature curves in the target irradiated by a multi-pulsed laser take on a delayed character in different sections away from the boundary, which is only affected by its relaxation time. The front of the stress wave is very steep in the non-Fourier numerical solutions, which presents an obvious thermal shock, so it is necessary to consider the non-Fourier effect of semi-infinite body under the high energy laser radiation.

关键词： multi-pulsed laser non-Fourier effect thermal shock

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