Mechanisms of Dangua Recipe in Improving Glycolipid Metabolic Disorders Based on Transcriptomics

作     者：HENG Xian-pei WANG Zhi-ta LI Liang YANG Liu-qing HUANG Su-ping HENG Xian-pei;WANG Zhi-ta;LI Liang;YANG Liu-qing;HUANG Su-ping

作者机构：Departme nt of Endocri no logyPeople's Hospital Affiliated to Fujian University of Traditional Chinese MedicineFuzhou(350004)China Academy of Integrative MedicineFujian University of Traditional Chinese MedicineFuzhou(350122)China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2022年第28卷第2期

页      面：130-137页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the National Natural Science Foundation of China(No.81873213,81473550) the Natural Science Foundation of Fujian Province(No.2017J01213,2016J0146) the Inn ovation Fund of Medical Science of Fujian Provi nee(No.2017-CX-42),China 

主　　题：transcriptomics Dangua Recipe glycolipid metabolism Nampt therapeutic target Chinese medicine 

摘      要：Objective:To explore the mechanisms of Dangua Recipe(DGR)in improving glycolipid metabolism based on ***:Sprague-Dawley rats with normal glucose level were divided into 3 groups according to a random number table,including a conventional diet group(Group A),a DGR group(Group B,high-calorie diet+20.5 g DGR),and a high-calorie fodder model group(Group C).After 12 weeks of intervention,the liver tissue of rats was *** sequence and transcriptional analysis were performed to identify the key genes related to glycolipid metabolism reflecting DGR efficacy,and then gene or protein validation of liver tissue were *** phosphoribosyl transferase(Nampt)and phosphoenolpyruvate carboxykinase(PEPCK)proteins in liver tissues were detected by enzyme linked immunosorbent assay,fatty acid synthase(FASN)protein was detected by Western blot,and fatty acid binding protein 5(FABP5)-mRNA was detected by quantitative real-time polymerase chain ***,the functional verification was performed on the diabetic model rats by Nampt blocker(GEN-617)injected in *** A1c(HbA1c),plasma total cholesterol and triglycerides were ***:Totally,257 differentialdominant genes of Group A *** C and 392 differential-dominant genes of Group B *** C were ***,11 Gene Ontology molecular function terms and 7 Kyoto Encyclopedia of Genes and Genomes enrichment pathways owned by both Group A *** C and Group C *** B were *** liver tissue target validation showed that Nampt,FASN,PEPCK protein and FABP5-mRNA had the same changes consistent with *** in vivo functional tests showed that GEN-617 increased body weight,HbA1c,triglyceride and total cholesterol levels in the diabetic rats(P0.05 or P0.01);while all the above-mentioned levels(except triglyceride)were decreased significantly by GEN-617 combined with DGR intervention(P0.05 or P0.01).Conclusion:Nampt activation was one of the