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Ethylene Response Factor TERF1 Enhances Glucose Sensitivity in Tobacco through Activating the Expression of Sugar-related Genes

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Journal of Integrative Plant Biology 2009年第2期51卷 184-193页

作者： Ang Li Zhijin Zhang Xue-Chen Wang rongfeng huang National Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100094 China Biotechnology Research Institute the Chinese Academy of Agricultural Sciences Beijing 100081 China National Key Facility of Crop Gene Resources and Genetic Improvement Beijing 100081 China National Center for Plant Gene Research (Beijing) Beijing 100081 China

Ethylene response factor （ERF） proteins are important plant-specific transcription factors. Increasing evidence shows that ERF proteins regulate plant pathogen resistance, abiotic stress response and plant development through interaction with different stress responsive pathways. Previously, we revealed that overexpression of TERF1 in tobacco activates a cluster gene expression through interacting with GCC box and dehydration responsive element （DRE）, resulting in enhanced sensitivity to abscisic acid （ABA） and tolerance to drought, and dark green leaves of mature plants, indicating that TERF1 participates in the integration of ethylene and osmotic responses. Here we further report that overexpression of TERF1 confers sugar response in tobacco. Analysis of the novel isolated tomato TERF1 promoter provides information indicating that there are many cis-acting elements, including sugar responsive elements （SURE） and W box, suggesting that TERF1 might be sugar inducible. This prediction is confirmed by results of reverse transcription-polymerase chain reaction amplification, indicating that transcripts of TERF1 are accumulated in tomato seedlings after application of glucose. Further investigation indicates that the expression of TERF1 in tobacco enhances sensitivity to glucose during seed germination, root and seedling development, showing a decrease of the fresh weight and root elongation under glucose treatment. Detailed investigations provide evidence that TERF1 interacts with the sugar responsive cis-acting element SURE and activates the expression of sugar response genes, establishing the transcriptional regulation of TERF1 in sugar response. Therefore, our results deepen our understanding of the glucose response mediated by the ERF protein TERF1 in tobacco.

关键词： Ethylene response factor protein TERF1 glucose sugar responsive gene tobacco

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Identification of integrin-like in guard cells of Vicia faba

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Chinese Science Bulletin 2001年第13期46卷 1100-1103页

作者： ZHANG Aihua huang rongfeng WANG Xuechen YUAN Ming College of Biological Sciences China Agricultural UniversityBeijing100094China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Key Lab of Plant Physiology&Biochemistry The Ministry of AgricultureBeijing 100094China

We addressed the existence and localization of integrin-like in guard cells of Vicia faba by using a probe of polyclonal antibody against the human integrin (αvβ3/β5)-Western blot results showed that three integrins-like of about 47.3, 43.7 and 41.1 ku were detected from the preparation of membrane fragments of purified guard cell protoplasts. Further research with immunofluorescent scanning microscopy indicated that those integrins-like were localized on plasma membrane of guard cells, most nearing the dorsal wall, which is consistent with the reception of signals from epidermal cells to guard cells. Thus our results indicate, for the first time, that integrins-like are present at guard cell plasma membrane of Vicia faba.

关键词： integrins-like guard cells Viciafaba signal transduction.

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Abscisic Acid Antagonizes Ethylene Production through the ABI4-Mediated Transcriptional Repression of ACS4 and ACS8 in Arabidopsis

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Molecular Plant 2016年第1期9卷 126-135页

作者： Zhijun Dong Yanwen Yu Shenghui Li Juan Wang Saijun Tang rongfeng huang College of Biological Sciences China Agricultural University Beijing 100193 China plant Molecular Biology Department Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 China National Key Facility of Crop Gene Resources and Genetic Improvement Beijing 100081 China These authors contributed equally to this article

Increasing evidence has revealed that abscisic acid （ABA） negatively modulates ethylene biosynthesis, although the underlying mechanism remains unclear. To identify the factors involved, we conducted a screen for ABA-insensitive mutants with altered ethylene production in Arabidopsis. A dominant allele of ABI4, abi4-152, which produces a putative protein with a 16-amino-acid truncation at the C-terminus of ABI4, reduces ethylene production. By contrast, two recessive knockout alleles of ABI4, abi4-102 and abi4-103, result in increased ethylene evolution, indicating that ABI4 negatively regulates ethylene produc- tion. Further analyses showed that expression of the ethylene biosynthesis genes ACS4, ACSS, and AC02 was significantly decreased in abi4-152 but increased in the knockout mutants, with partial dependence on ABA. Chromatin immunoprecipitation-quantitative PCR assays showed that ABI4 directly binds the pro- moters of these ethylene biosynthesis genes and that ABA enhances this interaction. A fusion protein containing the truncated ABI4-152 peptide accumulated to higher levels than its full-length counterpart in transgenic plants, suggesting that ABI4 is destabilized by its C terminus. Therefore, our results demon- strate that ABA negatively regulates ethylene production through ABI4-mediated transcriptional repression of the ethylene biosynthesis genes ACS4 and ACS8 in Arabidopsis.

关键词： ABA ABI4 ethylene biosynthesis stress response transcriptional regulation

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OsEIL1 and OsEIL2,two master regulators of rice ethylene signaling,promote the expression of ROS scavenging genes to facilitate coleoptile elongation and seedling emergence from soil

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Plant Communications 2024年第3期5卷 93-105页

作者： Jinzhu Qiao Ruidang Quan Juan Wang Yuxiang Li Dinglin Xiao Zihan Zhao rongfeng huang Hua Qin Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China National Key Facility of Crop Gene Resources and Genetic Improvement Beijing 100081China

Successful emergence from the soil is a prerequisite for survival of germinating seeds in their natural *** rice,coleoptile elongation facilitates seedling emergence and establishment,and ethylene plays an important role in this ***,the underlying regulatory mechanism remains largely ***,we report that ethylene promotes cell elongation and inhibits cell expansion in rice coleoptiles,result-ing in longer and thinner coleoptiles that facilitate seedlings emergence from the *** analysis showed that genes related to reactive oxygen species(ROS)generation are upregulated and genes involved in ROS scavenging are downregulated in the coleoptiles of ethylene-signaling *** investiga-tions showed that soil coverage promotes accumulation of ETHYLENE INSENSITIVE 3-LIKE 1(OsEIL1)and OsEIL2 in the upper region of the coleoptile,and both OsEIL1 and OsEIL2 can bind directly to the promoters of the GDP-mannose pyrophosphorylase(VTC1)gene OsVTC1-3 and the peroxidase(PRX)genes OsPRX37,OsPRX81,OsPRX82,and OsPRX88 to activate their *** leads to increased ascorbic acid content,greater peroxidase activity,and decreased ROS accumulation in the upper region of the *** of ROS accumulation promotes coleoptile growth and seedling emergence from *** deepen our understanding of the roles of ethylene and ROS in controlling coleoptile growth,and this information can be used by breeders to produce rice varieties suitable for direct seeding.

关键词： ethylene ROS coleoptile growth emergence rate rice

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Multiple transgenes Populus xeuramericana 'Guariento' plants obtained by biolistic bombardment

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Chinese Science Bulletin 2007年第2期52卷 224-230页

作者： Wang JianGe Su XiaoHua Ji LiLi Zhang BingYu Hu ZanMin huang rongfeng Tian YingChuan Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 China College of Landscape and art Jiangxi Agricultural University Nanchang 330045 China Institute of Genetics and developmental biology Chinese Academy of Sciences Beijing 100101 China Biotechnology Research Institute Chinese Academy of Agricultural Science Beijing 100081 China Microbiology Institute Chinese Academy of Sciences Beijing 100080 China

A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Populus xeuramericana 'Guariento' using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P. xeuramericana 'Guariento' and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land.

关键词：森林生态系统全球变暖二氧化碳植被

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High auxin stimulates callus through SDG8-mediated histone H3K36 methylation in Arabidopsis

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Journal of Integrative Plant Biology 2022年第12期64卷 2425-2437页

作者： Jun Ma Qiang Li Lei Zhang Sen Cai Yuanyuan Liu Juncheng Lin rongfeng huang Yongqiang Yu Mingzhang Wen Tongda Xu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002China Plant Synthetic Biology Center FAFU-UCR Joint Center for Horticultural Biology and MetabolomicsHaixia Institute of Science and TechnologyFujian Agriculture and Forestry UniversityFuzhou 350002China Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China Basic Forestry and Proteomics Research Center Haixia Institute of Science and TechnologyFujian Agricuture and Forestry UniversityFuzhou 350002China Frontiers Science Center for Synthetic Biology(Ministry of Education) Tianin UniversityTianjin 300072China

Callus induction,which results in fate transition in plant cells,is considered as the first and key step for plant *** process can be stimulated in different tissues by a callus-inducing medium(CIM),which contains a high concentration of phytohormone *** a few key regulators for callus induction have been identified,the multiple aspects of the regulatory mechanism driven by high levels of auxin still need further ***,we find that high auxin induces callus through a H3 K36 histone methylation-dependent mechanism,which requires the methyltransferase SET DOMAIN GROUP 8(SDG8).During callus induction,the increased auxin accumulates SDG8 expression through a TIR1/AFBs-based transcriptional ***8 then deposits H3 K36 me3 modifications on the loci of callus-related genes,including a master regulator WOX5 and the cell proliferation-related genes,such as *** epigenetic regulation in turn is required for the transcriptional activation of these genes during callus *** findings suggest that the massive transcriptional reprogramming for cell fate transition by auxin during callus formation requires epigenetic modifications including SDG8-mediated histone H3 K36 *** results provide insight into the coordination between auxin signaling and epigenetic regulation during fundamental processes in plant development.

关键词： auxin callus formation epigenetic regulation H3K36 methylation SDG8 TIR1/AFBs

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The chloroplast-localized protein LTA1 regulates tiller angle and yield of rice

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The Crop Journal 2022年第4期10卷 952-961页

作者： Xiaowu Pan Yongchao Li Haiwen Zhang Wenqiang Liu Zheng Dong Licheng Liu Sanxiong Liu Xinnian Sheng Jun Min rongfeng huang Xiaoxiang Li Rice Research Institute Hunan Academy of Agricultural SciencesChangsha 410125HunanChina Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China

Plant architecture strongly influences rice grain *** report the cloning and characterization of the LTA1 gene,which simultaneously controls tiller angle and yield of ***1 encodes a chloroplastlocalized protein with a conserved YbaB DNA-binding domain,and is highly expressed in photosynthetic tissues including leaves and leaf *** the function of LTA1 leads to large tiller angle and yield reduction of ***1 affects the gravity response by mediating the distribution of endogenous auxin,thereby regulating the tiller *** lta1 mutant showed abnormal chloroplast development and decreased chlorophyll content and photosynthetic rate,in turn leading to reduction of rice *** findings shed light on the genetic basis of tiller angle and provide a potential gene resource for the improvement of plant architecture and rice yield.

关键词： Rice Yield Tiller angle Gravitropism Chloroplast development

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木材塑性变形的湿热固定技术及机理研究进展

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林业科学 2022年第2期58卷 206-216页

作者：黄荣凤中国林业科学研究院木材工业研究所国家林业和草原局木材科学与技术重点实验室北京100091

塑性变形永久固定是木材压缩密实化、弯曲等塑性改性的关键科学和技术问题之一。本研究针对塑性变形湿热固定技术,从塑性变形形成和回复机理入手,在全面分析木材塑性变形固定研究现状的基础上,重点阐述木材塑性变形的湿热固定技术和机... 详细信息

塑性变形永久固定是木材压缩密实化、弯曲等塑性改性的关键科学和技术问题之一。本研究针对塑性变形湿热固定技术,从塑性变形形成和回复机理入手,在全面分析木材塑性变形固定研究现状的基础上,重点阐述木材塑性变形的湿热固定技术和机理研究进展以及存在的问题,为突破该项技术的产业化应用瓶颈提供参考。湿热固定技术是以释放木材内应力方式固定塑性变形的方法,因处理方式不同,区分为常压热处理和加压热处理两大类。目前采用的所有处理方法,永久固定塑性变形的必要条件为温度达到180℃以上,加快热传递速度,并使板材厚度方向的中心温度快速达到180℃,能够有效缩短塑性变形永久固定时间。湿热处理永久固定塑性变形机理研究主要从热处理和饱和蒸汽处理2种过程中木材内应力释放与化学性质变化之间的关联性展开,多数研究支持3种基本机制,即基质分子间的交联反应形成稳定的结构;分子链断裂使微纤维和基质中应力松弛;亲水的细胞壁成分,特别是半纤维素形成聚合物阻止其再次发生水软化。但这些研究实际上只局限在2个极端,一端是干燥状态下依靠提高温度和延长时间释放木材内应力的热处理,另一端是接近饱水状态下依靠高温高压释放木材内应力的饱和蒸汽处理,而且这些研究均未涉及影响塑性变形固定和木材性能热、质传递等重要的过程因素,存在永久固定和木材性能变化的相互制约等适用性问题。虽然采用湿热处理方法理论上已经实现塑性变形的永久固定,但这些技术并未实现产业化应用,主要原因在于支撑湿热处理永久固定塑性变形的理论和技术体系不完整。要解决塑性变形的湿热固定技术应用问题,不能只关注最终条件,还有考虑如何有效实现这些条件,因此,需从湿热处理过程中水分的作用、热作用和氧化作用及其相互作用机理,热、质传递规律,湿热作用下木材内应力释放的作用机制等方面开展深入研究。

关键词：塑性变形永久固定残余应力应力释放湿热处理

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Circulating exosome-like vesicles of humans with nondiabetic obesity impaired isletβ-cell proliferation,which was associated with decreased Omentin-1 protein cargo

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Genes & Diseases 2022年第4期9卷 1099-1113页

作者： Qian Ge Xinxin Xie Xiangjun Chen rongfeng huang Cheng-Xue Rui Qianna Zhen Renzhi Hu Min Wu Xiaoqiu Xiao Xi Li Department of Endocrinology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China The Biology Science Institutes Chongqing Medical UniversityChongqing 400016PR China The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China de Duve Institute Catholic University of LouvainBrussels 1200Belgium

The regulation ofβ-cell mass in the status of nondiabetic obesity remains not well *** aimed to investigate the role of circulating exosome-like vesicles(ELVs)isolated from humans with simple obesity in the regulation of isletβ-cell *** June 2017 and July 2019,81 subjects with simple obesity and 102 healthy volunteers with normal weight were *** were isolated by *** proliferations ofβ-cells and islets were measured by 5-ethynl-2′-deoxyuridine(EdU).Protein components in ELVs were identified by Quantitative Proteomic Analysis and verified by Western blot and *** role of specific exosomal protein was analyzed by gain-of-function approach in ELVs released by 3T3-L1 *** ELVs from subjects with simple obesity inhibitedβ-cell proliferation in vitro without affecting its apoptosis,secretion,and *** protein levels of Rictor and Omentin-1 were downregulated in circulating ELVs from subjects with simple obesity and associated with the obesity-linked pathologic *** ELV-carried Omentin-1 and Omentin-1 protein per se were validated to increaseβ-cell proliferation and activate Akt signaling ***,Omentin-1 in ELVs was downregulated by *** circulating ELVs may act as a negative regulator forβ-cell mass in nondiabetic obesity through inhibitingβ-cell *** effect was associated with downregulated Omentin-1 protein in *** newly identified ELV-carried protein could be a mediator linking insulin resistance to impairedβ-cell proliferation and a new potential target for increasingβ-cell mass in obesity and T2DM.

关键词： β-Cell Exosome Obesity Proliferation Type 2 diabetes mellitus

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Hippocampal overexpression of TREM2 ameliorates high fat diet induced cognitive impairment and modulates phenotypic polarization of the microglia

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Genes & Diseases 2022年第2期9卷 401-414页

作者： Min Wu Maolin Liao rongfeng huang Chunxiu Chen Tian Tian Hongying Wang Jiayu Li Jibin Li Yuxiang Sun Chaodong Wu Qifu Li Xiaoqiu Xiao Department of Endocrinology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China Department of Nutrition and Food Hygiene School of Public Health and ManagementChongqing Medical UniversityChongqing 400016PR China Department of Neurology Affiliated Hospital of Guizhou Medical UniversityGuiyangGuizhou 550004PR China Department of Nutrition and Food Science Texas A&M UniversityCollege StationTX 77843USA

Type 2 diabetes mellitus(T2DM)and Alzheimer's disease(AD)share several common pathophysiological *** variants of triggering receptor expressed on myeloid cells 2(TREM2)increase the risk of developing AD,suggesting the involvement of TREM2 and innate immunity in AD *** is still unknown whether TREM2 is related to cognitive impairment in ***,we investigated the effects of the hippocampal overexpression of TREM2 on cognitive in long-term high-fat diet(HFD)-fed *** C57BL/6J mice were maintained on HFD for 50 ***2 was overexpressed in the hippocampus 36 weeks after HFD feeding using adeno-associated virus vector(AAV)-mediated gene *** results showed that the HFD feeding induced rapid and persistent weight gain,glucose intolerance and significant impairments in learning and *** with AAV-con,AAV-TREM2 significantly ameliorated cognitive impairment without altering body weight and glucose homeostasis in HFD *** overexpression of TREM2 upregulated the synaptic proteins spinophilin,PSD95 and synaptophysin,suggesting the improvement in synaptic *** complexity and spine density in the CA1 region were rescued after TREM2 ***,TREM2 markedly increased the number of iba-1/Arg-1-positive microglia in the hippocampus,suppressed neuroinflammation and microglial *** sum,hippocampal TREM2 plays an important role in improving HFD-induced cognitive dysfunction and promoting microglial polarization towards the M2 anti-inflammatory *** study also suggests that TREM2 might be a novel target for the intervention of obesity/diabetes-associated cognitive decline.

关键词： Diabetes Microglial polarization Neurodegeneration Neuroinflammation Obesity TREM2

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