检索结果-南通市图书馆

Zoological Research 2020年第4期41卷 423-430页

作者： Zhong-Yuan Shen Dan Yu Xin Gao Fu-Tie zhang huan-zhang liu The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences Institute of HydrobiologyChinese Academy of SciencesWuhanHubei 430072China University of Chinese Academy of Sciences Beijing 100049China

The Chinese sturgeon(Acipenser sinensis Gray,1835)is a large anadromous fish species,which is under considerable threat due to dramatic declines in population numbers.In the current study,population genetic diversity and individual reproductive success were assessed using nuclear microsatellite markers(simple sequence repeat,SSR)and complete mitochondrial(mt DNA)genome analysis of juveniles born in 2014.Results showed the existence of size polymorphism in the mt DNA genome of Chinese sturgeon,which was caused by a repeat motif.Population genetic diversity was high based on both SSR(Ho:0.728±0.211;He:0.779±0.122)and mt DNA genome analyses(H:0.876±0.0035;Pi:0.0011±0.0010).A positive inbreeding coefficient(FIS:0.066±0.143)was also found,indicating the occurrence of inbreeding.Reconstruction of sibling groups identified 11 mothers and 11 fathers involved in reproduction of Chinese sturgeons in 2014.Variance in individual reproductive success was not significant,with reproductive success of parent fish instead shown to be relatively even(P=0.997>0.05),thus suggesting the absence of sweepstakes reproductive success(SRS).These results indicate that,in regard to conservation,loss of genetic diversity due to the effects of SRS is not of particular concern.However,we must focus on having an adequate number of adults and suitable environmental conditions to ensure that fish can reproduce.

关键词： instead absence Yangtze

Evolution and phylogenetic application of the MC1R gene in the Cobitoidea(Teleostei: Cypriniformes)

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2016年第5期37卷 281-289页

作者： Qiong-Ying TANG Li-Xia SHI Fei liu Dan YU huan-zhang liu The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China University of Chinese Academy of Sciences Beijing 100049 China

Fish of the superfamily Cobitoidea sensu stricto （namely Ioaches） exhibit extremely high diversity of color patterns, but so far little is known about their evolutionary mechanism. Melanocortin 1 receptor gene （MCIR） plays an important role during the synthesis of melanin and formation of animal body color patterns. In this study, we amplified and sequenced the partial MCIR gene for 44 loach individuals representing 31 species of four families. Phylogenetic analyses yielded a topology congruent with previous studies using multiple nuclear loci, showing that each of the four families was monophyletic with sister relationships of Botiidae＋（Cobitidae＋（Balitoridae＋Nemacheilidae））. Gene evolutionary analyses indicated that MCIR in Ioaches was under purifying selection pressure, with various sites having different dNIds values. Both Botiidae and Cobitidae had lower dN/ds values than those of background lineages, suggesting their evolution might be strongly affected by purifying selection pressure. For Balitoddae and Nemacheilidae, both had larger dNIds values than those of background lineages, suggesting they had a faster evolutionary rate under more relaxed selection pressure. Consequently, we inferred that the relatively stable color patterns in Botiidae and Cobitidae might result from the strong purifying selection pressure on the MC1R gene, whereas the complicated and diverse color patterns in Balitoridae and Nemacheilidae might be associated with the relaxed selection pressure. Given the easy experimental procedure for the partial MCTR gene and its excellent performance in reconstructing phylogeny, we suggest this gene could be used as a good molecular marker for the phylogenetic study of fish species.

关键词： Gene evolution Molecular phylogeny Cobitoidea Melanocortin 1 receptor gene

Genetic diversity and temporal changes of an endemic cyprinid fish species, Ancherythroculter nigrocauda,from the upper reaches of Yangtze River

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2019年第5期40卷 427-438页

作者： Dong-Dong Zhai Wen-Jing Li huan-zhang liu Wen-Xuan Cao Xin Gao Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences Institute of Hydrobiology Chinese Academy of Sciences Wuhan Hubei 430072 China University of Chinese Academy of Sciences Beijing 100049 China

Small populations with low genetic diversity are prone to extinction. Knowledge on the genetic diversity and structure of small populations and their genetic response to anthropogenic effects are of critical importance for conservation management. In this study, samples of Ancherythroculter nigrocauda, an endemic cyprinid fish from the upper reaches of Yangtze River, were collected from five sites to analyze their genetic diversity and population structure using mitochondrial cytochrome b gene and 14 microsatellite loci. Haplotype diversity, nucleotide diversity, and expected heterozygosity indicated that the A. nigrocauda populations had low genetic diversity, and decreased heavily from 2001 to 2016. Significant genetic differentiation was found among different populations in the cyt b gene and SSR markers based on the genetic differentiation index (F ST), whereas no differentiation was found in 2001. Haplotype genealogy showed that eight out of 15 haplotypes were private to one population. The SSR STRUCTURE analysis showed that there were four genetic clusters in the A. nigrocauda samples, with each population forming a single cluster, except for the Chishui River (CSR) and Mudong River (MDR) populations, which formed a common cluster. Therefore, loss of genetic diversity and increased genetic differentiation were found in the A. nigrocauda populations, which could be attributed to dam construction, overfishing, and water pollution in the upper Yangtze River. It is therefore recommended that the government should ban fishing, control water pollution, increase river connectivity, and establish artificial breeding and stocking.

关键词： Genetic diversity Population structure Temporal change Conservation

Discovery of a wild,genetically pure Chinese giant salamander creates new conservation opportunities

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2022年第3期43卷 469-480页

作者： Jing Chai Chen-Qi Lu Mu-Rong Yi Nian-Hua Dai Xiao-Dong Weng Ming-Xiao Di Yong Peng Yong Tang Qing-Hua Shan Kai Wang huan-zhang liu Hai-Peng Zhao Jie-Qiong Jin Ru-Jun Cao Ping Lu Lai-Chun Luo Robert W.Murphy Ya-Ping zhang Jing Che State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China Institute of Biological Resources Jiangxi Academy of SciencesNanchangJiangxi 330096China Jiangxi Jiulingshan National Nature Reserve Jing’anJiangxi 330600China Sam Noble Oklahoma Museum of Natural History&Department of Biology University of OklahomaNorman 73072USA The Key Laboratory of Biodiversity and Conservation of Aquatic Organisms Institute of HydrobiologyChinese Academy of SciencesWuhanHubei 430072China School of Life Science Henan UniversityKaifengHenan 475001China Jing’an County Bureau of Agriculture and Rural Affairs Jing’anJiangxi 330600China Centre for Biodiversity and Conservation Biology Royal Ontario MuseumTorontoOntario M5S 2C6Canada Yunnan Key Laboratory of Biodiversity and Ecological Security of Gaoligong Mountain Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China Kunming College of Life Science University of the Chinese Academy of SciencesKunmingYunnan 650204China

Effective conservation of threatened biota relies on accurate assessments and scientific guidance.As an unfortunate example,Chinese giant salamanders(Andrias,CGS)remain critically endangered in nature.Misguided conservation efforts,e.g.,commercial propagation and releasing of millions of likely non-indigenous or interspecific hybrids,have further compromised conservation initiatives.Limited information on wild populations of CGS poses a significant conservation challenge.Following 18-month long field monitoring,we now report the discovery of a wild population of CGS in a closed nature reserve in Jiangxi Province,China.Genomic assessments reveal its genetic distinctiveness and do not detect genetic admixture with other species.Based on morphological and molecular evidences,we describe this CGS as a new species Andrias jiangxiensis sp.nov.This is the only known species of CGS today with a genetically pure,reproducing,in situ population.This discovery emphasizes the important role that closed nature reserves play in protecting species,and the necessity of integrating long-term field monitoring and genetic assessments.It sets a new pathway for discovering and conserving endangered species,especially for those biotas that are similarly being extirpated by anthropogenic translocations and overexploitation.

关键词： Conservation Human translocation Genetic homogenization Field monitoring Taxonomy

Multivariate statistical and bioinformatic analyses for the seasonal variations of actinobacterial community structures in a drinking water reservoir

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第3期137卷 1-17页

作者： Haihan zhang Sixuan Pan BenMa Tinglin huang Dmitry B.Kosolapov Manli Ma Xiang liu huan liu Xiaoyan liu Shaanxi Key Laboratory of Environmental Engineering Key Laboratory of Northwest Water ResourceEnvironment and EcologyMOEXi’an University of Architecture and TechnologyXi’an 710055China School of Environmental and Municipal Engineering Xi’an University of Architecture and TechnologyXi’an 710055China Papanin Institute for Biology of Inland Waters of Russian Academy of Sciences(IBIW RAS) 109BorokNekouzYaroslavl152742Russia

Actinobacterial community is a conspicuous part of aquatic ecosystems and displays an important role in the case of biogeochemical cycle,but little is known about the seasonal variation of actinobacterial community in reservoir ecological environment.In this study,the high-throughput techniques were used to investigate the structure of the aquatic actinobacterial community and its inducing water quality parameters in different seasons.The results showed that the highest diversity and abundance of actinobacterial community occurred in winter,with Sporichthya(45.42%)being the most abundant genus and Rhodococcus sp.(29.32%)being the most abundant species.Network analysis and correlation analysis suggested that in autumn the dynamics of actinobacterial community were infuenced by more factors and Nocardioides sp.SX2R5S2 was the potential keystone species which was negatively correlated with temperature(R=-0.72,P<0.05).Changes in environmental factors could significantly affect the changes in actinobacterial community,and the dynamics of temperature,dissolved oxygen(DO),and turbidity are potential conspicuous factors infuencing seasonal actinobacterial community trends.The partial least squares path modeling further elucidated that the combined effects of DO and temperature not only in the diversity of actinobacterial community but also in other water qualities,while the physiochemical parameters(path coefficient=1.571,P<0.05)was strong environmental factors in natural mixture period.These results strengthen our understanding of the dynamics and structures of actinobacterial community in the drinking water reservoirs and provide scientific guidance for further water quality management and protection in water sources.

关键词： Source water reservoir High-throughput sequencing Correlation analysis Network analysis Partial least squares path modeling

Oncogenic MORC2 in cancer development and beyond

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第2期11卷 861-873页

作者： Shan zhang Ayao Guo huan Wang Jia liu Chenshuang Dong Junyi Ren Guiling Wang Key Laboratory of Cell Biology Department of Cell BiologyMinistry of Public Health and Key Laboratory of Medical Cell BiologyMinistry of EducationChina Medical UniversityShenyangLiaoning 110122China Department of Breast Surgery The First Affiliated Hospital of China Medical UniversityShenyangLiaoning 110001China

Microrchidia CW-type zinc finger 2(MORC2)is a member of the MORC superfamily of nuclear proteins.Growing evidence has shown that MORC2 not only participates in gene transcription and chromatin remodeling but also plays a key in human disease and tumor development by regulating the expression of downstream oncogenes or tumor suppressors.The present review provides an updated overview of MORC2 in the aspect of cancer hallmark and therapeutic resistance and summarizes its upstream regulators and downstream target genes.This systematic review may provide a favorable theoretical basis for emerging players of MORC2 in tumor development and new insight into the potential clinical application of basic science discoveries in the future.

关键词： Cancer development Hallmarks of cancer MORC2 Therapeutic target Treatment resistance

Outer-surface Covering of Nanochannels with Hydrogel for Highly Sensitive and Specific Cr(VI)Detection Through Analyte-caused Charge Change in Hydrogel

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2024年第2期40卷 326-332页

作者： liu huan SUN Xueting DAI Yu zhang Xiaojin XIA Fan Faculty of Materials Science and Chemistry China University of GeosciencesWuhan430074P.R.China

Nanochannels have made great progress and are a promising platform for detecting a series of targets.However,most nanochannels are modified on the inner wall,while ignoring the outer surface.Here,we modified the outer surface of nanochannels with hydrogel.Different from other reported outer-surface modification methods,we directly cover nanochannels with hydrogel to form heterogeneous membrane.The selected hydrogel hardly adsorbs other ions and shows specific adsorption for Cr(VI).The adsorption sites in hydrogel are homogeneous,and Cr(VI)adsorption onto hydrogel is endothermic and spontaneous.The charge in hydrogel changes after Cr(VI)adsorption,and the resulting current changes can be used for the detection of Cr(VI)with the detection limit of 10−11 mol/L.Our platform is expected to be used for Cr(VI)detection in living organisms,especially within cells.This work provides a new approach for outer-surface modification of nanochannels and offers a new choice for nanochannel detection platforms.

关键词： Nanochannel Hydrogel Heterogeneous membrane Cr(VI)detection

Real-Time Co-optimization of Gear Shifting and Engine Torque for Predictive Cruise Control of Heavy-Duty Trucks

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Mechanical Engineering 2024年第2期37卷 294-317页

作者： Hongqing Chu Xiaoxiang Na huan liu Yuhai Wang Zhuo Yang Lin zhang Hong Chen School of Automotive Studies Tongji UniversityShanghaiChina Department of Engineering University of CambridgeCambridgeUK State Key Laboratory of Automotive Simulation and Control Jilin UniversityChangchunChina Qingdao Automotive Research Institute Jilin UniversityQingdaoChina Dongfeng Commercial Vehicle Technology Center Dongfeng Motor CorporationWuhanChina

Fuel consumption is one of the main concerns for heavy-duty trucks.Predictive cruise control(PCC)provides an intriguing opportunity to reduce fuel consumption by using the upcoming road information.In this study,a real-time implementable PCC,which simultaneously optimizes engine torque and gear shifting,is proposed for heavy-duty trucks.To minimize fuel consumption,the problem of the PCC is formulated as a nonlinear model predictive control(MPC),in which the upcoming road elevation information is used.Finding the solution of the nonlinear MPC is time consuming;thus,a real-time implementable solver is developed based on Pontryagin’s maximum principle and indirect shooting method.Dynamic programming(DP)algorithm,as a global optimization algorithm,is used as a performance benchmark for the proposed solver.Simulation,hardware-in-the-loop and real-truck experiments are conducted to verify the performance of the proposed controller.The results demonstrate that the MPC-based solution performs nearly as well as the DP-based solution,with less than 1%deviation for testing roads.Moreover,the proposed co-optimization controller is implementable in a real-truck,and the proposed MPC-based PCC algorithm achieves a fuel-saving rate of 7.9%without compromising the truck’s travel time.

关键词： Heavy-duty truck Predictive cruise control Model predictive control Pontryagin’s maximum principle Real-truck implementation

LncRNA NKILA inhibits HBV replication by repressing NF-κB signalling activation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2024年第1期39卷 44-55页

作者： Xi zhang Yuanyuan Li Chen huan Yubao Hou Rujia liu Hongyun Shi Peng zhang Baisong Zheng Yingchao Wang Hong Wang Wenyan zhang Institute of Virology and AIDS Research The First Hospital of Jilin UniversityChangchun130012China Department of Ophthalmology The First Hospital of Jilin UniversityChangchun130012China Hepatobiliary Pancreatic Surgery The First Hospital of Jilin UniversityChangchun130012China

Hepatitis B virus(HBV)infection results in liver cirrhosis and hepatocellular carcinoma(HCC).HBx/nuclear factor(NF)-κB pathway plays a role in HBV replication.However,whether NF-κB-interacting long noncoding RNA(NKILA),a suppressor of NF-κB activation,regulates HBV replication remains largely unknown.In this study,gain-and-loss experiments showed that NKILA inhibited HBV replication by inhibiting NF-κB activity.In turn,HBV infection down-regulated NKILA expression.In addition,expression levels of NKILA were lower in the peripheral blood-derived monocytes(PBMCs)of HBV-positive patients than in healthy individuals,which were correlated with HBV viral loads.And a negative correlation between NKILA expression level and HBV viral loads was observed in blood serum from HBV-positive patients.Lower levels of endogenous NKILA were also observed in HepG2 cells expressing a 1.3-fold HBV genome,HBV-infected HepG2-NTCP cells,stable HBV-producing HepG2.2.15 and HepAD38 cells,compared to those HBV-negative cells.Furthermore,HBx was required for NKILA-mediated inhibition on HBV replication.NKILA decreased HBx-induced NF-κB activation by interrupting the interaction between HBx and p65,whereas NKILA mutants lack of essential domains for NF-κB inhibition,lost the ability to inhibit HBV replication.Together,our data demonstrate that NKILA may serve as a suppressor of HBV replication via NF-κB signalling.

关键词： LncRNA NF-κB-interacting longnoncoding RNA(NKILA) NF-ĸB signalling ChronichepatitisBvirus(HBV) Viral replication HBx

Experimental Study on Towing Characteristics of Composite Bucket Wellhead Platform

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

China Ocean Engineering 2024年第3期38卷 531-542页

作者： zhang Pu-yang liu Ying-fei LE Cong-huan DING Hong-yan BAI Yu State Key Laboratory of Hydraulic Engineering Intelligent Construction and Operation Tianjin UniversityTianjin 300072China

With the rapid development of large-scale development of marginal oilfields in China,simple wellhead platforms that are simple in structure and easy to install have become an inevitable choice in the process of oilfield development.However,traditional simple wellhead platforms are often discarded after a single use.In pursuit of a more costeffective approach to developing marginal oilfields,this paper proposes a new offshore oil field development facility—an integrated bucket foundation for wellhead platform.To verify the safety of its towing behavior and obtain the dynamic response characteristics of the structure,this paper takes a bucket integrated bucket foundation for wellhead platform with a diameter of 40 m as the research object.By combining physical model tests and numerical simulations,it analyzes the static stability and dynamic response characteristics of the structure during towing,complete with the effects of the draft,wave height,wave period,and towing point height,which produce the dynamic responses of the structure under different influence factors,such as roll angle,pitch angle,heave acceleration and towing force as well as the sensibility to transport variables.The results show that the integrated bucket foundation for wellhead platform is capable of self-floating towing,and its movement is affected by the local environment,which will provide a reference for actual projects.

关键词： composite bucket foundation wellhead platform air flotation towing model test