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Preliminary results of CCD observations targeting Himalia acquired at Yunnan Observatories in 2015

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Research in Astronomy and Astrophysics 2016年第12期16卷 59-66页

作者： huan-Wen peng Na Wang Qing-Yu peng Sino-French Joint Laboratory for Astrometry Dynamics and Space ScienceJinan UniversityGuangzhou 510632China Yunnan Observatories Chinese Academy of SciencesKunming 650216China University of Chinese Academy of Sciences Beijing 100049China

In order to study the potential associated with high precision CCD astrometry of irregular satel- lites, we have acquired experimental observations of Himalia, the sixth and irregular satellite of Jupiter. A total of 185 CCD observations were obtained by using the 2.4m and 1 m telescopes administered by Yunnan Observatories over ten nights. Preliminary analysis of the observations were made, including geo- metric distortion, atmospheric refraction, and also the phase effect. All positions of Himalia are measured relative to the reference stars from the catalog UCAC4 in each CCD field of view. The theoretical positions of Himalia were retrieved from the Institute de M6chanique C61este et de Calcul des t^ph6m6rides, while the positions of Jupiter were obtained based on the planetary ephemeris INPOP13c. The results show that the means of observed minus computed （O - C） residuals are -0.004＂ and -0.002＂ in right ascension and declination, respectively. The standard deviations of （O - C） residuals are estimated to be about 0.04＂ in each direction.

关键词： astrometry -- planets and satellites: individual (Himalia) -- methods: observational

An improved solution to geometric distortion using an orthogonal method

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Research in Astronomy and Astrophysics 2017年第2期17卷 89-96页

作者： huan-Wen peng Qing-Yu peng Na Wang Yunnan Observatories Chinese Academy of Sciences Kunming 650216 China Department of Computer Science Jinan University Guangzhou 510632 China University of Chinese Academy of Sciences Beijing 100049 China Sino-French Joint Laboratory for Astrometry Dynamics and Space Science Jinan University Guangzhou 510632 China

The geometric distortion of a CCD field of view has a direct influence on the positional measurements of CCD observations. In order to obtain high precision astrometric results, the geometric distortion should be derived and corrected precisely. As presented in our previous work, a convenient solution has been carried out and has also been applied to observations of Phoebe. In order to fur- ther improve the solution, an orthogonal method based on Zernike polynomials is used in this work. Four nights of CCD observations including Himalia, the sixth satellite of Jupiter, and open clusters （NGC 1664 or NGC 2324） on each night have been processed as an application. The observations were obtained from the 2.4 m telescope administered by Yunnan Observatories. The catalog UCAC4 was used to match reference stars in all of the CCD frames. The ephemeris of Himalia is retrieved from the Institut de M6canique C61este et de Calcul des Ephemerides （IMCCE）. Our results show that the means of observed minus calculated （O - C） positional residuals are -0.034 and -0.026 arcsec in right ascension and declination, respectively. The corresponding standard deviations are 0.031＂ and 0.028＂. The measurement dispersion is significantly improved compared to that by using our previous solution.

关键词： astrometry -- planets and satellites: individual (Himalia) -- methods: observational

A novel chorismate mutase effector secreted from root-knot nematode Meloidogyne enterolobii manipulates plant immunity to promote parasitism

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Journal of Integrative Agriculture 2024年第12期23卷 4107-4119页

作者： Tuizi Feng Yuan Chen Zhourong Li Ji Pei Deliang peng huan peng Haibo Long Key Laboratory of Integrated Pest Management on Tropical Crops Ministry of Agriculture and Rural AffairsEnvironment and Plant Protection InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101China Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests Environment and Plant Protection InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China

Meloidogyne *** an economically important plant-parasitic nematode distributed *** fight with host immune system for successful parasitism,plant parasitic nematodes secrete effectors to promote *** this study,we identified one chorismate mutase(CM)effector from ***,named *** and temporal expression assays exhibited Me-cm is expressed in esophageal glands and up-regulated at parasitic-stage ***-CM affects the pathogenicity of *** based on the reduced infection rate,number of galls,egg masses,eggs per mass and multiplication rate collected from RNA silencing *** showed that Me-CM localized in the cytoplasm and nucleus of plant cells and decreased the expression level of the marker gene PR1 of salicylic acid(SA)***,constitutive expression of Me-cm in Arabidopsis thaliana significantly reduced salicylic acid *** results suggested that *** may secrete effector Me-CM to fight with plantimmunesystemsvia regulating SA signaling pathway when interacting with host plants,ultimately facilitating parasitism.

关键词： Meloidogyne enterolobii effector chorismate mutase salicylic acid plant immunity

Effect of Aspergillus niger NBC001 on the soybean rhizosphere microbial community in a soybean cyst nematode-infested field

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Journal of Integrative Agriculture 2021年第12期20卷 3230-3239页

作者： JIN Na LIU Shi-ming peng huan huanG Wen-kun KONG Ling-an peng De-liang Department of Horticulture Beijing Vocational College of AgricultureBeijing 102442P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Soybean cyst nematode(SCN,Heterodera glycines Ichinohe)is one of the most important pests causing considerable damage to soybean(Glycine max(L.)Merr.)around the *** provides a strategy for sustainable nematode ***,Aspergillus niger NBC001 was isolated from the cysts Heterodera *** able to control *** and promote the growth of soybean in a pot *** this study,the effects of NBC001 on *** density and on the soybean rhizosphere microbial community in a soybean cyst nematode-infested field were *** results showed that NBC001 could suppress *** by 31.7%in the ***-throughput sequencing analysis showed that NBC001 had no significant influence on soybean rhizosphere microbial community structure,indicating that seed coatdressing with the concentrated culture filtrate of NBC001 was safe for the soil ecological *** addition,high-throughput sequencing results demonstrated that at 10 days post transplantation,NBC001 increased the abundances of Actinobacteria and Acidobacteria,but decreased the abundances of Bacteroidetes and Gemmatimonadetes at the phylum ***,the abundances of Phyllobacterium,Ralstonia and H16 were increased,while the abundances of Adhaeribacter,Gemmatimonas,Sphingomonas,Flavisolibacter\Nere suppressed by application of ***,at 90 days post transplantation,NBC001 only increased the abundances oi Aeromicrobium and RB41 whereas it decreased the abundance of *** results indicated that application of NBC001 increased the relative abundances of the beneficial microorganisms such as Actinobacteria,Acidobacteria,Aeromicrobium and Phyllobacterium in the *** summary,NBC001 is an eco-friendly biocontrol agent for *** control.

关键词： Aspergillus niger NBC001 biocontrol Heterodera glycines high-throughput sequencing microbial community

Molecular and morphological characterization of stunt nematodes of wheat, maize, and rice in the savannahs of northern Nigeria

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Journal of Integrative Agriculture 2022年第2期21卷 586-595页

作者： Sulaiman ABDULSALAM peng huan LIU Shi-ming huanG Wen-kun KONG Ling-an peng De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193P.R.China Division of Agricultural Colleges Department of Crop ProtectionAhmadu Bello UniversityZaria 810107Nigeria

Stunt nematodes(Tylenchorhynchus spp.)are obligate migratory root ecto-parasitic nematodes found in the fields of many cultivated *** nematodes,with phyto-sanitary potential,are frequently ignored or misdiagnosed as pests,and this may pose a threat to food *** accuracy of its identification based on a morphological approach has been challenged recently,due to the overlapping of the morphological and morphometric characters of the ***,the objective of this study is to identify and characterize stunt nematodes present in 54 fields cultivated with cereal crops(wheat,maize and rice)in the savannahs of northern Nigeria,using integrative taxonomy and molecular *** molecular and morphological studies identified and confirmed the presence of *** as the occurring specie in the savannahs of northern *** phylogenetic analysis was carried out using the internal transcribed spacer(ITS)and 28S genes of ribosomal DNA further confirmed the presence of *** first molecular characterization and sequences of the ITS and 28S rDNA gene for *** from Nigeria were provided by this ***,according to our literature search,this is the first report on *** in wheat,maize and rice in the savannahs of northern *** study to test the pathogenicity of the parasitic nematode species found in this survey is recommended for the prioritization and development of efficient management strategies.

关键词： plant-parasitic nematodes food security Tylenchorhynchus annulatus ITS phylogeny

Molecular diagnosis and direct quantification of cereal cyst nematode(Heterodera filipjevi) from field soil using TaqMan real-time PCR

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Journal of Integrative Agriculture 2023年第8期22卷 2591-2601页

作者： JIAN Jin-zhuo huanG Wen-kun KONG Ling-an JIAN Heng Sulaiman ABDULSALAM peng De-liang peng huan State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Key Laboratory of Plant Pathology of Ministry of Agriculture and Rural Affairs College of Plant ProtectionChina Agricultural UniversityBeijing100193P.R.China Division of Agricultural Colleges/Department of Crop Protection Ahmadu Bello UniversityZaria 810107Nigeria

Heterodera filipjevi continues to be a major threat to wheat production *** detection and quantification of cyst nematodes are essential for more effective control against this nematode *** the present study,a TaqManminor groove binder(TaqMan-MGB)probe-based fluorescence quantitative real-time PCR(qPCR)was successfully developed and used for quantifying *** from DNA extracts of *** primers and probe designed from the obtained RAPD-SCAR marker fragments of *** showed high specificity to *** using DNA from isolatesconfirmed species of 23 Heterodera spp.,1 Globodera *** 3 Pratylenchus *** qPCR assay is highly sensitive and provides improved *** detection sensitivity of as low as 4^(-3) single second-stage juvenile(J2)DNAs,10^(-3) female DNAs,and 0.01μgμL^(-1) genomic DNAs.A standard curve relating to the threshold cycle and log values of nematode numbers was generated and validated from artificially infested soils and was used to quantify *** in naturally infested field *** was a high correlation between the *** numbers estimated from 32 naturally infested field soils by both conventional methods and the numbers quantified using the qPCR *** potentially provides a useful platform for the efficient detection and quantification of *** directly from field soils and to quantify this species directly from DNA extracts of field soils.

关键词： cereal cyst nematode Heterodera filipjevi molecular diagnosis quantification TaqMan real-time PCR

Influence of supercritical CO_(2)on the physical property of tight sandstone

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Petroleum 2024年第3期10卷 520-526页

作者： huan peng Jian Yang Junliang peng Huifen Han Xinghao Gou Yucheng Jia Yibo Li Valeriy Kadet Engineering Technology Research Institute of Southwest Oil&Gas Field Company PetroChinaChengdu610017China Key Laboratory of Oil&Gas Well Stimulation Technology of Southwest Oil&Gas Field Company Chengdu610017China State Key Laboratory of Oil&Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengdu610500China Gubkin Russia State University of Oil and Gas Moscow119991Russia

In low-pressure gas reservoirs,water-based fracture fluid is difficult to flowback,which is unfavorable for several tight sandstone gas reservoirs in the Sichuan Basin with low pressure and high permeability geological *** CO_(2)possesses a number of remarkable physical and chemical features,including a density near to water,a viscosity close to gas,and high *** CO_(2)fracturing is a new type of non-aqueous fracturing method that is favorable to fracturing flowback in low-pressure tight sandstone and has a wide range of *** discuss on whether supercritical CO_(2)fracturing with low pressure tight sandstone is *** sandstone cores from the Jinqiu gas field in the Sichuan Basin were used to study the influence of supercritical CO_(2)on the physical properties of sandstone *** CO_(2)was used to interact with tight sandstone samples,and then the changes in porosity,permeability,and rock microstructure of tight sandstone were observed under various time,pressure,and temperature *** the interaction between tight sandstone and supercritical CO_(2),new dissolution pores will appear,or the original pores will be increased,and the width of some natural fractures will also be increased,and the porosity will increase by 1.09%-8.85%,and the permeability will increase by 2.34%-21.26%,quantifying the influence of supercritical CO_(2)on physical properties of tight sandstone,and further improving the interaction mechanism between supercritical CO_(2)and tight *** study improves in the understanding of the tight sandstone-supercritical CO_(2)interaction mechanism,as well as providing an experimental foundation and technological guarantee for field testing and use of supercritical CO_(2)in low-pressure tight sandstone gas reservoirs.

关键词： Supercritical CO_(2) Low pressure tight sandstone Porosity Permeability Microstructure

Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene(Ha-acp1) from Heterodera avenae

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Journal of Integrative Agriculture 2014年第6期13卷 1303-1310页

作者： LIU Yan-ke huanG Wen-kun LONG Hai-bo peng huan HE Wen-ting peng De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands cells always play an important role during the early parasitic process. A new acid phosphatase gene （Ha-acp1） expressed in the subventral glands of the cereal cyst nematode （Heterodera avenae） was cloned and the characteristics of the gene were analyzed. Results showed that the gene had a putative signal peptide for secretion and in situ hybridization showed that the transcripts of Ha-acp1 accumulated speciifcally in the subventral gland cells of H. avenae. Southern blot analysis suggested that Ha-acp1 belonged to a multigene family. RT-PCR analysis indicated that this transcription was strong at the pre-parasitic juveniles. Knocking down Ha-acp1 using RNA interference technology could reduce nematode infectivity by 50%, and suppress the development of cyst. Results indicated that Ha-acp1 could play an important role in destroying the defense system of host plants.

关键词： Heterodera avenae acid phosphatase RNA interference

Assaying the potential of twenty-one legume plants in Medicago truncatula and M. sativa for candidate model plants for investigation the interactions with Heterodera glycines

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Journal of Integrative Agriculture 2016年第3期15卷 702-704页

作者： KONG Ling-an WU Du-qing huanG Wen-kun peng huan HE Wen-ting peng De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

Soybean cyst nematode Heterodera glycines is one of the most serious soil-borne pathogens in soybean production. However, the researches were limited in China due to lack of an effective pathosystem. In this study, we screened 21 legume Medicago plants in both Medicago truncatula and Medicago sativa to obtain candidate model plants for establishing a new pathosystem for legume-H. glycines interactions. The nematode infection of tested plants was assayed with Race 3 and 4 respectively, which were two dominant H. glycines inbred races in China soybean producing areas. The results showed that the model legume plant M. truncatula A17 failed to allow Race 3 of H. glycines to complete its life cycle, in contrast, it provided the Race 4 population to form several cyst nematodes, however, the female index（FI） value was approximately 1.6. Three M. sativa cultivars, including Xunlu, Aergangjin and Junren, provided either Race 3 or 4 of H. glycines to develop into mature cysts with their FI value below 5 as well. Our results demonstrated that legume plants in both M. truncatula and M. sativa were not likely to be a model plant for H. glycines because of an extreme high resistance.

关键词： soybean cyst nematode alfalfa life cycle female index pathosystem