检索结果-南通市图书馆

Binding sensitivity of adefovir with different genotypes of HBV polymerase:molecular modeling,docking and dynamics simulation studies

在线全文

学校读者我要写书评

暂无评论

Binding sensitivity of adefovir with different genotypes of ...

增加吸气时间对腹腔镜手术患者气体交换和呼吸力学的影响

中国化学会第28届学术年会

作者： Jing Li,Yun Du,Xian Liu,Qiancheng Shen,Mingyue Zheng*,Xiaomin luo,hualiang Jiang Shanghai Institute of Materia Medica,Chinese Academy of Sciences,201203,Shanghai

＜正＞Adefovir is a nucleos（t）ide analogue reverse transcriptase inhibitor （NRTIs）, which has been evaluated as primary monotherapy for patients with chronic hepatitis B.

关键词： HBV polymerase genotype adefovir molecular dynamics simulation free energy calculation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

医学研究杂志 2014年第6期43卷 128-131,178页

作者：张群钱祖超罗华梁浙江省临海市第二人民医院麻醉科 317016

目的探讨延长吸气时间即反比通气对腹腔镜手术患者气体交换的影响.方法择期腹腔镜下妇科手术患者75例,按照随机数字表法分为3组：传统的吸呼比1∶2组,吸呼比1∶1组,吸呼比2∶1组,每组各25例.潮气量设定为6～7ml/kg,吸呼比在气腹开始... 详细信息

目的探讨延长吸气时间即反比通气对腹腔镜手术患者气体交换的影响.方法择期腹腔镜下妇科手术患者75例,按照随机数字表法分为3组：传统的吸呼比1∶2组,吸呼比1∶1组,吸呼比2∶1组,每组各25例.潮气量设定为6～7ml/kg,吸呼比在气腹开始时进行调整.于下列时间点测定动脉血气：麻醉诱导后15min（T1）,CO2气腹开始后25min（T2）,50min（T3）时,并计算相应时间点氧合指数（PaO2/FiO2）和生理无效腔（VD/VT）;记录相应时点呼吸力学和血流动力学指标.结果 1∶2组T2、T3时点PaO2/FiO2较T1时有所下降（P＜0.05）,而1∶1组和2∶1组PaO2/FiO2无显著下降.3组PaCO2在T2、T3时点均明显增加,但T3时,2∶1组PaCO2比1∶1组和1∶2组低（P＜0.05）.与1∶2组比较,T2、T3时点,1∶1组和2∶1组VD/VT显著降低（P＜0.05）.气腹开始后所有组气道峰压和气道平台压均升高;与1∶2组相应时点比较,1∶1组和2∶1组T2、T3时点Pplat上升（P＜0.05）;T2和T3时,1∶1组和2∶1组的吸气峰流量较在T1时明显降低.手术开始后呼吸系统静态和动态顺应性降低,但组间无统计学差异（P＞0.05）.结论腹腔镜手术时延长吸气时间有助于改善氧合.与常规吸呼比比较,延长吸气时间不增加气道峰压或气道平台压且能加快CO2的排出,反比通气可促进气体交换和改善呼吸功能.

关键词：腹腔镜气体交换吸气时间影响

丙泊酚复合不同镇痛药物用于无痛人工流产术的效果及对体动的影响

在线全文

学校读者我要写书评

暂无评论

中国基层医药 2018年第23期25卷 2998-3001页

作者：罗华梁崔敏杰李玉丽施红英临海市第二人民医院麻醉科浙江省临海317000 温州医科大学临床医学系浙江省温州325035

目的比较丙泊酚复合地佐辛与丙泊酚复合芬太尼麻醉用于无痛人工流产术的临床效果及对体动的影响。方法选择临海市第二人民医院2016年7月至2017年5月自愿行无痛人工流产术的患者240例,患者年龄范围19~45岁,采用随机数字表法分为三组,每... 详细信息

目的比较丙泊酚复合地佐辛与丙泊酚复合芬太尼麻醉用于无痛人工流产术的临床效果及对体动的影响。方法选择临海市第二人民医院2016年7月至2017年5月自愿行无痛人工流产术的患者240例,患者年龄范围19~45岁,采用随机数字表法分为三组,每组80例。D组给予丙泊酚复合地佐辛麻醉,F组给予丙泊酚复合芬太尼麻醉,C组作为对照给予单纯丙泊酚静脉麻醉,三组术中根据具体情况追加丙泊酚。比较三组手术时间、苏醒时间及丙泊酚总用量,比较三组术后0min、15min、30min、1 h、3 h视觉疼痛模拟评分(VAS评分),观察三组患者体动、呼吸抑制等不良反应发生情况。结果D组手术时间、苏醒时间、丙泊酚总用量分别为(4.5±0.7)min、(1.2±0.3)min、(136.8±14.5)mg,F组分别为(4.6±0.8)min、(1.3±0.2)min、(139.6±12.1)mg,两组差异均无统计学意义(均P>0.05);D组、F组苏醒时间、丙泊酚总用量均低于C组[苏醒时间(2.4±0.6)min、丙泊酚总用量(168.7±18.9)mg](t=25.298、27.287、11.977、11.598,均P<0.05);D组术后0min、15min、30min、1 h、3 h的VAS评分均低于F组、C组(t=34.785、25.044、25.043、22.349、28.622、48.809、42.278、40.000、28.527、29.145,均P<0.05),F组术后0min、15min、30min、1 h的VAS评分均低于C组(t=36.978、24.406、19.846、8.016,均P<0.05)。D组恶心、体动、呼吸抑制、低血压发生率分别为1.3%(1例)、2.5%(2例)、1.3%(1例)、0.0%(0例),F组分别为0.0%(0例)、3.8%(3例)、3.8%(3例)、2.5%(2例),D组、F组均低于C组的6.3%(5例)、16.3%(13例)、11.3%(9例)、15.0%(12例)(χ2=3.925、3.657、3.925、5.889、5.889、3.152、3.152、3.657,均P<0.05)。结论丙泊酚复合地佐辛与芬太尼用于无痛人工流产术效果均较佳,且体动反应少,但复合地佐辛术后疼痛程度轻。

关键词：麻醉和镇痛流产,人工丙泊酚

Insight into Urban Faults by Wavelet Multi-Scale Analysis and Modeling of Gravity Data in Shenzhen,China

在线全文

学校读者我要写书评

暂无评论

Journal of Earth Science 2018年第6期29卷 1340-1348页

作者： Chuang Xu Haihong Wang Zhicai luo hualiang Liu Xiangdong Liu MOE Key Laboratory of Fundamental Physical Quantities Measurement School of PhysicsHuazhong University of Science and Technology School of Geodesy and Geomatics Wuhan University Key Laboratory of Geospace Environment and Geodesy Ministry of EducationWuhan University China Water Resources Beifang Investigation Design and Research Co.Ltd.

Urban faults in Shenzhen are potential threats to city security and sustainable development. In consideration of the importance of the Shenzhen fault zone, the author provide a detailed interpretation on gravity data model. Bouguer gravity covering the whole Shenzhen City was calculated with a 1-km resolution. Wavelet multi-scale analysis（MSA） was applied to the Bouguer gravity data to obtain the multilayer residual anomalies corresponding to different depths. In addition, 2D gravity models were constructed along three profiles. The Bouguer gravity anomaly shows an NE-striking high-low-high pattern from northwest to southeast, strongly related to the main faults. According to the results of MSA, the correlation between gravity anomaly and faults is particularly significant from 4 to 12 km depth. The residual gravity with small amplitude in each layer indicates weak tectonic activity in the crust. In the upper layers, positive anomalies along most of faults reveal the upwelling of high-density materials during the past tectonic movements. The multilayer residual anomalies also yield important information about the faults, such as the vertical extension and the dip direction. The maximum depth of the faults is about 20 km. In general, NE-striking faults extend deeper than NW-striking faults and have a larger dip angle.

关键词： urban faults Bouguer gravity anomaly wavelet multi-scale analysis gravity modeling Shenzhen

Studies on Ecological Monitoring of Plant Community and Plant Diversity in Shenzhen, Southern China

在线全文

学校读者我要写书评

暂无评论

Open Journal of Forestry 2021年第4期11卷 352-380页

作者： Yuyuan Huang Xinfan Yu Hong Liang Zhiming Yang Haiyang Wen Zhen Ye Yunhe Lu Ruoyu Wei Lijun Yang Song Ma Kangsai Zhao Fan Wang Zhijie Chen Huina Yang Chengxi Jiang Bin Xu Weimin Wang Wang Xu Zhu Ming Shizhen Lin Hao Liu hualiang luo Suni Deng Lijuan Huang Yanjun Lei Xiaoli Wan Yahe Li Wanmin Zhou Shun Zhao Zhenliang Duan Dongyao Liao Xiaobo Qiu Haoqun Huang Linlin Jiang College of Agriculture and Biology Zhongkai University of Agriculture and Engineering Guangzhou China Shenzhen Environmental Monitoring Center Shenzhen China South China Institute of Environmental Sciences Ministry of Ecology and Environment Guangzhou China Environmental Monitoring Station of Nanshan District Shenzhen China Ecological and Environmental Protection Monitoring Station of Dapeng District Shenzhen China Construction Public Service Bureau of Dapeng New District Shenzhen China

From Jan. 2013 to Nov. 2018, we carried out the investigation on vegetation studies in the ecological monitoring programs, Shenzhen and totally researched 170 plant communities of mountain areas etc., few areas of street regions and parks etc., for communities’ structural characteristics and plant diversity. We comparatively analyzed natural forests, semi-natural forest and artificial forest composition and structural characters and family, genus and species diversity level and the main affected factors. We also researched and analyzed these communities’ structural characteristics and pant diversity, comparatively analyzed natural forests, semi-natural forest and artificial forest composition and structural characters and family, genus and species diversity level, the main affected factors;researched and analyzed the relationship between plant community structure characteristics with the absorbing ability to PM2.5 pollutants. The result showed that the structure and plant diversity of natural forest were obviously better than artificial disturbed forest or artificial forest, the semi-natural forest was the middle level of the forests, In the indices of height, DBH, coverage and crow width etc. of tree layer, natural forests and semi-natural forests were obvious

关键词： Shenzhen Vegetation Community Structure Diversity PM2.5 Ecological Monitoring Natural Forest Artificial Forest

Steered molecular dynamics simulations of protein-ligand interactions

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2004年第5期47卷 355-366页

作者： XU Yechun SHEN Jianhua luo Xiaomin SHEN Xu CHEN Kaixian JIANG hualiang Center for Drug Discovery and Design State Key Laboratory of New Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China Center for Drug Discovery and Design State Key Laboratory of New Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China

Studies of protein-ligand interactions are helpful to elucidating the mechanisms of ligands, providing clues for rational drug design. The currently developed steered molecular dy- namics (SMD) is a complementary approach to experimental techniques in investigating the biochemical processes occurring at microsecond or second time scale, thus SMD may provide dynamical and kinetic processes of ligand-receptor binding and unbinding, which cannot be ac- cessed by the experimental methods. In this article, the methodology of SMD is described, and the applications of SMD simulations for obtaining dynamic insights into protein-ligand interactions are illustrated through two of our own examples. One is associated with the simulations of bind- ing and unbinding processes between huperzine A and acetylcholinesterase, and the other is concerned with the unbinding process of α-APAfrom HIV-1 reverse transcriptase.

关键词： molecular dynamics simulation, steered molecular dynamics simulation, atomic force microscope, avidin, biotin,huperzine A, acetylcholinesterase, HIV-1 reverse transcriptas, non-nucleoside RT inhibitor.

Research progress in cation-π interactions

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2008年第8期51卷 709-717页

作者： CHENG JiaGao luo XiaoMin YAN XiuHua LI Zhong TANG Yun JIANG hualiang ZHU WeiLiang School of Pharmacy East China University of Science and TechnologyShanghai 200237China Drug Discovery and Design Center Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China School of Sciences East China University of Science and TechnologyShanghai 200237China

Cation-π interaction is a potent intermolecular interaction between a cation and an aromatic system,which has been viewed as a new kind of binding force,as being compared with the classical interactions(e.g. hydrogen bonding,electrostatic and hydrophobic interactions). Cation-π interactions have been observed in a wide range of biological contexts. In this paper,we present an overview of the typical cation-π interactions in biological systems,the experimental and theoretical investigations on cation-π interactions,as well as the research results on cation-π interactions in our group.

关键词： cation-π interaction intermolecular interaction noncovalent interaction

Determining PPARg-ligand binding affinity using fluorescent assay with cis-parinaric acid as a probe

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2005年第2期48卷 122-131页

作者： GAO Zhenting luo Haibin CHEN Lili SHEN Jianhua CHEN Kaixian JIANG hualiang SHEN Xu Drug Discovery and Design Center Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Upon the study of small-molecules binding to proteins, the traditional methods for calculating dissociation constants (Kd and Ki) have shortcomings in dealing with the single bind- ing site models. In this paper, two equations have been derived to solve this problem. These two equations are independent of the total concentration or initial degree of saturation of receptor and the activity of the competitive molecule. Through nonlinear fitting against these two equations, Kd value of a probe can be obtained by binding assay, and Ki value of a ligand can be obtained by competitive assay. Moreover, only the total concentrations of receptor([R]t), ligand([L]t) and probe([P]t) are required for the data fitting. In this work, Ki values of some typical ligands of PPARγ were successfully determined by use of our equations, among which the Ki value of PPARγ-LY171883 was reported for the first time.

关键词： displacement assay IC_(50) equilibrium dissociation constant PPAR cis-parinaric acid fluorescent probe

High-resolution mapping of brain vasculature and its impairment in the hippocampus of Alzheimer's disease mice

在线全文

学校读者我要写书评

暂无评论

National Science Review 2019年第6期6卷 1223-1238页

作者： Xiaochuan Zhang Xianzhen Yin Jingjing Zhang Anan Li Hui Gong Qingming luo Haiyan Zhang Zhaobing Gao hualiang Jiang School of Pharmacy East China University of Science and Technology CAS Key Laboratory of Receptor Research Shanghai Institute of Materia MedicaChinese Academy of Sciences Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and Technology MoE Key Laboratory for Biomedical Photonics School of Engineering Sciences Huazhong University of Science and Technology Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and TechnologyShanghai Tech University

Accumulating evidence indicates the critical importance of cerebrovascular dysfunction in the pathogenesis of Alzheimer’s disease(AD). However, systematic comparative studies on the precise brain vasculature of wild-type and AD model mice are still rare. Using an image-optimization method for analysing Micro-Optical Sectioning Tomography(MOST) data, we generated cross-scale whole-brain 3 D atlases that cover the entire vascular system from large vessels down to smallest capillaries at submicron resolution,for both wild-type mice and a transgenic(APP/PS1) mouse model of AD. In addition to distinct vascular patterns in different brain regions, we found that the main vessels of the molecular layer of the hippocampal dentate gyrus(DG-ml) undergo abrupt changes in both diameter and branch angle, spreading a unique comb-like pattern of capillaries. By using a quantitative analysis workflow, we identified in the hippocampus of AD mice an overall reduction of the mean vascular diameter, volume fraction and branch angle, with most significant impairment in the DG-ml. In addition, virtual endoscopy revealed irregular morphological features in the vessel lumen of the AD mice, potentially contributing to the impairment of blood flow. Our results demonstrate the capability of high-resolution cross-scale evaluation of brain vasculature and underscore the importance of studying hippocampal microcirculation for understanding AD pathogenesis.

关键词： Micro-Optical Sectioning Tomography image optimization Alzheimer’s disease impaired hippocampal vasculature virtual vascular endoscopy