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An inactivated recombinant rabies virus chimerically expressed RBD induces humoral and cellular immunity against SARS-CoV-2 and RABV

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Virologica Sinica 2023年第2期38卷 244-256页

作者： Haili Zhang Hongli Jin Feihu Yan Yumeng Song Jiaxin Dai Cuicui Jiao Yujie Bai Jingxuan sun Di Liu Shen Wang Mengyao Zhang Jilong Lu Jingbo Huang Pei Huang Yuanyuan Li Xianzhu Xia hualei Wang Key Laboratory of Zoonosis Research Ministry of EducationCollege of Veterinary MedicineJilin UniversityChangchun130062China Changchun Veterinary Research Institute Chinese Academy of Agricultural SciencesChangchun130122China Changchun Sino Biotechnology Co. Ltd.Changchun130012China

Many studies suggest that severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)can infect various animals and transmit among animals,and even to humans,posing a threat to humans and *** is an urgent need to develop inexpensive and efficient animal vaccines to prevent and control coronavirus disease 2019(COVID-19)in *** virus(RABV)is another important zoonotic pathogen that infects almost all warmblooded animals and poses a great public health *** present study constructed two recombinant chimeric viruses expressing the S1 and RBD proteins of the SARS-CoV-2 Wuhan01 strain based on a reverse genetic system of the RABV SRV9 strain and evaluated their immunogenicity in mice,cats and *** results showed that both inactivated recombinant viruses induced durable neutralizing antibodies against SARS-CoV-2 and RABV and a strong cellular immune response in ***,inactivated SRV-nCoV-RBD induced earlier antibody production than SRV-nCoV-S1,which was maintained at high levels for longer *** SRV-nCoV-RBD induced neutralizing antibodies against both SARS-CoV-2 and RABV in cats and dogs,with a relatively broadspectrum cross-neutralization capability against the SARS-CoV-2 pseudoviruses including Alpha,Beta,Gamma,Delta,and Omicron,showing potential to be used as a safe bivalent vaccine candidate against COVID-19 and rabies in animals.

关键词： SARS-CoV-2 Vaccine RBD Chimeric expression RABV

The Application of a Safe Neutralization Assay for Ebola Virus Using Lentivirus-Based Pseudotyped Virus

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Virologica Sinica 2021年第6期36卷 1648-1651页

作者： Zengguo Cao Hongli Jin Gary Wong Ying Zhang Cuicui Jiao Na Feng Fangfang Wu Shengnan Xu Hang Chi Yongkun Zhao Tiecheng Wang Weiyang sun Yuwei Gao Songtao Yang Xianzhu Xia hualei Wang Key Laboratory of Zoonosis Research Ministry of EducationCollege of Veterinary MedicineJilin UniversityChangchun 130062China Key Laboratory of Jilin Province for Zoonosis Prevention and Control Institute of Military VeterinaryAcademy of Military Medical SciencesChangchun 130122China Institut Pasteur of Shanghai Chinese Academy of SciencesShanghai 200031China Département de Microbiologie-Infectiologie Et D'immunologie UniversitéLavalQuébecQC G1V 0A6Canada Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose Yangzhou UniversityYangzhou 225009China

Dear Editor,Zaire Ebola virus(EBOV)is a negative-sense singlestranded RNA virus that is capable of causing acute hemorrhagic fever with a frightening fatality rate that can reach up to 90%.Due to its high lethality and frequent recurrence,EBOV is a substantial threat to public *** about live EBOV is restricted to BSL-4 laboratories;therefore,conventional serological detection methods do not provide a safe way to evaluate neutralizing activity,resulting in a bottleneck in antibody-based detection of viruses such as ***,we aim to develop and apply a neutralization assay for EBOV using a generated lentivirus-based pseudotyped EBOV bearing GP on its surface.

关键词： Ebola acute hemorrhagic

Design of Vibrio 16S rRNA Gene Specific Primers and Their Application in the Analysis of Seawater Vibrio Community

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Journal of Ocean University of China 2006年第2期5卷 157-164页

作者： LIU Yong YANG Guanpin WANG hualei CHEN Jixiang SHI Xianming ZOU Guiwei WEI Qiwei sun Xiuqin College of Marine Life Sciences Ocean University of China Qingdao 266003 P.R. China Department of Food Science and Technology Shanghai Jiaotong University Shanghai 201101 P.R. China Yangtse River Fishery Institute Chinese Academy of Fishery Jingzhou 434000 P.R. China The First Institute of Oceanography National Oceanic Administration of China Qingdao 266061 P.R. China

The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation.

关键词： Vibrio vibriosis bacterial community primer

吉林延边天宝山矿集区的叠加成矿——来自同位素年代学证据

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岩石学报 2014年第7期30卷 2081-2091页

作者：孙振明任云生鞠楠赵华雷陈聪孙宇超吉林大学地球科学学院长春130061 沈阳地质矿产研究所沈阳110034 吉林省龙井市瀚丰矿业有限公司龙井133400

天宝山大型多金属矿集区位于兴蒙造山带东缘,西拉木伦-长春断裂带与敦(化)-密(山)断裂带交汇部位的北东侧,已发现矽卡岩型Pb-Zn-Ag矿化(如立山和选厂后山)、隐爆角砾岩中的Pb-Zn-Ag矿化(如新兴)、沉积变质-热液改造型CuPb-Zn矿化(如东... 详细信息

天宝山大型多金属矿集区位于兴蒙造山带东缘,西拉木伦-长春断裂带与敦(化)-密(山)断裂带交汇部位的北东侧,已发现矽卡岩型Pb-Zn-Ag矿化(如立山和选厂后山)、隐爆角砾岩中的Pb-Zn-Ag矿化(如新兴)、沉积变质-热液改造型CuPb-Zn矿化(如东风南山)和热液脉型Mo矿化(如东风北山)等多种矿化类型。为建立该区的成岩成矿年代学格架,采用LAICP-MS单颗粒锆石U-Pb法,对立山成矿闪长岩与东风北山含矿黑云母石英闪长岩开展了同位素测年研究;并对东风北山钼矿床开展了辉钼矿Re-Os法测年。结果表明,立山闪长岩体与东风北山黑云母石英闪长岩体中锆石的加权平均年龄分别为266.2±3.9Ma(MSWD=14)和278.4±1.8Ma(MSWD=2.9),两岩体均属海西晚期岩浆事件的结果;东风北山钼矿床中6件辉钼矿样品的Re-Os模式年龄介于190.3±3.1Ma^194.6±2.8Ma,等时线年龄为192±3.1Ma,表明钼的成矿作用发生于燕山早期。立山铅锌矿化属接触交代型,成矿岩体的年龄基本代表成矿时代,所以立山铅锌矿床形成于海西晚期;东风北山钼矿床为热液脉型,虽然含矿岩体的侵位时代为海西期,但钼矿化发生于燕山早期,成矿与矿区深部或外围的燕山早期侵入体有关。因此,天宝山矿集区至少发生过海西期和燕山期两期大规模岩浆成矿事件。不同期次构造-岩浆-成矿作用的叠加应是该矿床矿化类型复杂、矿种多和规模大的主因。

关键词：锆石U-Pb法测年辉钼矿Re-Os测年矽卡岩型多金属矿化热液脉型钼矿化叠加成矿天宝山矿集区延边

补硒对载脂蛋白E基因敲除小鼠动脉粥样硬化的影响

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中华心血管病杂志 2016年第3期44卷 244-249页

作者：董华蕾袁娜孙涛顿爱社侯海峰山东省泰山医院心内科泰安271000 泰山医学院基础医学院泰山医学院公共卫生学院山东省医药卫生重点实验室

目的探讨补充硒元素对小鼠动脉粥样硬化的影响和对血管内皮功能的作用.方法将低硒高脂饲料饲养的载脂蛋白E基因敲除小鼠按照随机数字表法,分为对照组（未补硒,10只）、低剂量补硒组（饮用水的亚硒酸钠浓度为0.1 mg/L,10只）和高剂量... 详细信息

目的探讨补充硒元素对小鼠动脉粥样硬化的影响和对血管内皮功能的作用.方法将低硒高脂饲料饲养的载脂蛋白E基因敲除小鼠按照随机数字表法,分为对照组（未补硒,10只）、低剂量补硒组（饮用水的亚硒酸钠浓度为0.1 mg/L,10只）和高剂量补硒组（饮用水的亚硒酸钠浓度为0.2 mg/L,10只）.饲养15周后,测定各组小鼠心脏和肝脏中的硒含量、血脂水平、抗氧化功能指标[包括血清超氧化物歧化酶（SOD）活性、谷胱甘肽过氧化物酶（GSH-Px）活性和丙二醛水平]和血管内皮功能指标[包括血清一氧化氮（NO）和内皮素1、血管内皮生长因子（VEGF）水平和主动脉根部内皮素1和VEGF的表达水平],并采用油红O染色分析主动脉根部的动脉粥样硬化斑块面积.结果（1）高剂量补硒组和低剂量补硒组小鼠心脏和肝脏的硒含量均高于对照组（P均＜0.05）.（2）3组之间的血清甘油三酯、总胆固醇、低密度脂蛋白胆固醇、极低密度脂蛋白胆固醇和高密度脂蛋白胆固醇水平差异均无统计学意义（P均＞0.05）.（3）高剂量补硒组和低剂量补硒组的血清SOD活性分别为（152.3 ±11.3） U/ml和（113.8±12.5） U/ml,均高于对照组的（90.7±10.7）U/ml,且高剂量补硒组的血清SOD活性高于低剂量补硒组（P均＜0.05）;高剂量补硒组和低剂量补硒组的血清GSH-Px活性分别为（69.6±8.7）U/ml和（53.9±7.2）U/ml,均高于对照组的（36.4±5.6） U/ml,且高剂量补硒组的血清GSH-Px活性高于低剂量补硒组（P均＜0.05）;高剂量补硒组和低剂量补硒组的血清丙二醛水平分别为（4.13±1.21） nmol/ml和（4.73±1.05） nmol/ml,均低于对照组的（5.97±1.08） nmol/ml,且高剂量补硒组的血清MDA水平低于低剂量补硒组（P均＜0.05）.（4）高剂量补硒组和低剂量补硒组的血清NO水平分别为（79.0±14.6）μmol/L和（61.5±12.8）μmol/L,均高于对照组的（42.7 ±9.1）μmol/L,且高剂量补硒组的血清NO水平高于低剂量补硒组（P均＜0.05）;高剂量补硒组和低剂量补硒组的血清内皮素1水平分别为（46.3±4.7）ng/L和（52.8±6.3）ng/L,均低于对照组的（72.2±6.3）ng/L（P均＜0.05）,且高剂量补硒组与低剂量补硒组之间差异无统计学意义（P＞0.05）;高剂量补硒组和低剂量补硒组的血清VEGF水平分别为（83.5±22.0） ng/L和（97.4±16.5）ng/L,均低于对照组的（125.8±18.6） ng/L（P均＜0.05）,且高剂量补硒组与低剂量补硒组之间差异无统计学意义（P＞0.05）;高剂量补硒组和低剂量补硒组的主动脉根部内皮素1和VEGF的蛋白表达水平均低于对照组（P均＜0.05）.（5）高剂量补硒组和低剂量补硒组主动脉根部动脉粥样硬化斑块面积分别为（0.75 ±0.15）＆#215;105 μm2和（0.95 ±0.19）＆#215;105 μm2,均小于对照组的（1.13±0.23）＆#215;105 μm2,且高剂量补硒组斑块面积小于低剂量补硒组（P均＜0.05）.结论补硒可降低载脂蛋白E基因敲除小鼠的脂质过氧化程度,保护血管内皮功能,降低动脉粥样硬化的病变程度.

关键词：动脉粥样硬化硒脂质过氧化作用

Incorporation of Escherichia coli heat-labile enterotoxin B subunit into rabies virus particles enhances its immunogenicity in mice and dogs

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Biosafety and Health 2023年第5期5卷 308-319页

作者： Zhiyuan Gong Hailun Li Meichen Qian Yujie Bai Hongli Jin Jingxuan sun Mengyao Zhang Cuicui Jiao Pei Huang Yuanyuan Li Haili Zhang hualei Wang State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases Key Laboratory for Zoonosis Research of the Ministry of EducationInstitute of Zoonosisand College of Veterinary MedicineJilin UniversityChangchun 130062China Changchun Sino Biotechnology Co. Ltd.Changchun 130012China

Although inactivated vaccines against rabies have the advantage of high safety,effective protection against rabies virus(RABV)infection often requires multiple,high-dose *** a molecular adjuvant into the viral particles has been found to be a useful strategy to promote the immune effectiveness of inactivated *** this study,we constructed a recombinant virus,rCVS11-LTB,which chimerically expresses a molecular adjuvant heat-labile enterotoxin B subunit(LTB)protein on the surface of the RABV *** in vivo was found to be promoted by rCVS11-LTB through the activation of dendritic cells(DCs).Our results demonstrated that inactivated rCVS11-LTB was able to induce higher levels of virusneutralizing antibodies(VNAs)in both mice and dogs than the parent virus rCVS11,to enhance the cellular immune response and T cell immune memory in mice,and was also able to provide 100%protection in mice from lethal doses of rabies virus,indicating its potential as a safe and effective inactivated rabies vaccine candidate.

关键词： RABV Inactivated vaccine Incorporation LTB Dendritic cells Activation

Generation of 84-fs pulses from a mode-locked Tm：CNNGG disordered garnet crystal laser

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Photonics Research 2018年第8期6卷 800-804页

作者： ZHONGBEN PAN YICHENG WANG YONGGUANG ZHAO hualei YUAN XIAOJUN DAI HUAQIANG CAI JI EUN BAE sun YOUNG CHOI FABIAN ROTERMUND XAVIER MATEOS JOSEP MARIA SERRES PAVEL LOIKO UWE GRIEBNER VALENTIN PETROV Max Born Institute for Nonlinear Optics and Short Pulse Spectroscopy Max-Bom-Str. 2a D-12489 Berlin Germany Institute of Chemical Materials China Academy of Engineering Physics Mianyang 621900 China Jiangsu Key Laboratory of Advanced Laser Materials and Devices Jiangsu Normal University Xuzhou 221116 China Department of Physics Korea Advanced Institute of Science and Technology (KAIST) 34141 Daejeon South Korea Fisica i Cristal·lografia de Materials i Nanomaterials (FiCMA-FiCNA)-EMaS Departament de Quimica Fisica i Inorganica Universitat Rovira i Virgili (URV) Campus Sescelades E-43007 Tarragona Spain ITMO University 49 Kronverkskiy Pr. 197101 St. Petersburg Russia

A mode-locked laser based on a Tm:CNNGG disordered crystal as an active medium and a single-walled carbon nanotube saturable absorber is demonstrated, operating at a central wavelength of 2018 nm. Transformlimited 84 fs pulses are generated with an average output power of 22 mW at a repetition rate of ~90 MHz.A maximum output power of 98 mW is obtained at a slightly longer pulse duration of 114 fs.

关键词：无序晶体纳米管通讯技术发展现状

感染伪狂犬病病毒对小鼠脑细胞周期及相关因子的影响

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黑龙江畜牧兽医 2023年第3期 67-72页

作者：孙伟刘杉杉李华磊铜仁职业技术学院农学院贵州铜仁554300 民族中兽药分离纯化技术国家地方联合工程研究中心贵州铜仁554300 铜仁市畜牧技术推广站贵州铜仁554300

为了研究感染猪伪狂犬病病毒(Pseudorabies virus,PRV)对小鼠脑细胞周期及相关因子的影响,试验将40只6周龄Balb/c小鼠随机分成对照组和36小时组、48小时组、60小时组,试验组经皮下接种含量为1×10^(3)TCID_(50)/100μL的PRV溶液200μL,... 详细信息

为了研究感染猪伪狂犬病病毒(Pseudorabies virus,PRV)对小鼠脑细胞周期及相关因子的影响,试验将40只6周龄Balb/c小鼠随机分成对照组和36小时组、48小时组、60小时组,试验组经皮下接种含量为1×10^(3)TCID_(50)/100μL的PRV溶液200μL,对照组注射等量生理盐水。试验采用流式细胞术检测脑细胞周期变化,并采用实时荧光定量PCR和Western-blot方法分别检测细胞周期相关因子基因和蛋白质的表达情况。结果表明:与对照组相比,感染PRV后36小时,处于G0/G1期的细胞比例极显著升高,而处于S期的细胞比例则极显著降低(P0.05);感染PRV后36,48小时,cyclin D基因相对表达量极显著降低(P<0.01);而在感染PRV后36小时时cyclin E基因相对表达量显著上升(P<0.05),60小时时极显著上升(P<0.01)。此外,感染PRV后36小时,CDK4和CDK6蛋白相对表达量出现了下调;感染PRV后36,48小时,cyclin D蛋白处于下调表达,在感染后60小时略有上调;而在整个观察期内,cyclin E蛋白一直处于上调表达。说明感染PRV的早期,宿主可通过调节多种细胞因子间相互作用将细胞周期阻滞在G0/G1期。

关键词：伪狂犬病毒小鼠脑细胞周期阻滞

伪狂犬病病毒对小鼠脾脏细胞焦亡相关因子表达的影响

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黑龙江畜牧兽医 2022年第13期 89-94,134页

作者：孙伟刘杉杉高俊波李华磊铜仁职业技术学院农学院贵州铜仁554300 民族中兽药分离纯化技术国家地方联合工程研究中心贵州铜仁554300 铜仁市畜牧技术推广站贵州铜仁554300

为了研究感染猪伪狂犬病病毒(Pseudorabies virus,PRV)对小鼠脾脏细胞焦亡相关因子的影响,试验将40只Balb/c小鼠随机分为对照组和试验组(包括48小时组、72小时组、96小时组),试验组小鼠皮下接种1×10TCID/100μL PRV 200μL,对照组小鼠... 详细信息

为了研究感染猪伪狂犬病病毒(Pseudorabies virus,PRV)对小鼠脾脏细胞焦亡相关因子的影响,试验将40只Balb/c小鼠随机分为对照组和试验组(包括48小时组、72小时组、96小时组),试验组小鼠皮下接种1×10TCID/100μL PRV 200μL,对照组小鼠注射等量生理盐水。采集小鼠脾脏,采用酶联免疫吸附试验检测脾脏组织中白细胞介素-1β(interleukin-1β,IL-1β)和白细胞介素-18(interleukin-18,IL-18)水平;分别采用实时荧光定量PCR和Western-blot方法,检测细胞焦亡相关因子核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、天冬氨酸蛋白水解酶1(Caspase-1)、Gasdermins家族蛋白D(GSDMD)、IL-1β和IL-18基因和蛋白表达水平;采用免疫组织化学方法检测脾脏组织中Caspase-1和IL-β蛋白表达量。结果表明:与对照组相比,48,72,96小时组脾脏组织中IL-1β极显著升高(P<0.01);48小时组IL-18显著升高(P<0.05),72,96小时组极显著升高(P<0.01)。感染PRV后48小时组NLRP3基因相对表达量极显著升高(P<0.01),72,96小时组显著升高(P<0.05);48,72小时组Caspase-1基因相对表达量显著升高(P<0.05),96小时组极显著升高(P<0.01);96小时组GSDMD基因相对表达量极显著升高(P<0.01);72,96小时组IL-1β基因相对表达量极显著升高(P<0.01);48,72小时组IL-18基因相对表达量显著升高(P<0.05),96小时组极显著升高(P<0.01)。与对照组比较,48,72,96小时组小鼠脾脏细胞中NLRP3、Caspase-1和IL-18蛋白相对表达量均呈现上升趋势,而IL-1β蛋白在72,96小时,GSDMD蛋白在96小时时呈现明显上升趋势。免疫组织化学法进一步证实,Caspase-1和IL-β蛋白在小鼠脾脏中呈上调表达。说明PRV感染小鼠后,可通过上调表达细胞焦亡相关因子促进炎性反应的发生。

关键词：伪狂犬病病毒小鼠脾脏细胞焦亡炎性反应