Incorporation of Escherichia coli heat-labile enterotoxin B subunit into rabies virus particles enhances its immunogenicity in mice and dogs

作     者：Zhiyuan Gong Hailun Li Meichen Qian Yujie Bai Hongli Jin Jingxuan Sun Mengyao Zhang Cuicui Jiao Pei Huang Yuanyuan Li Haili Zhang Hualei Wang Zhiyuan Gon;Hailun Li;Meichen Qian;Yujie Bai;Hongli Jin;Jingxuan Sun;Mengyao Zhang;Cuicui Jiao;Pei Huang;Yuanyuan Li;Haili Zhang;Hualei Wang

作者机构：State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious DiseasesKey Laboratory for Zoonosis Research of the Ministry of EducationInstitute of Zoonosisand College of Veterinary MedicineJilin UniversityChangchun 130062China Changchun Sino Biotechnology Co.Ltd.Changchun 130012China 

出 版 物：《Biosafety and Health》 (生物安全与健康（英文）)

年 卷 期：2023年第5卷第5期

页      面：308-319页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 0836[工学-生物工程] 10[医学] 

基　　金：the National Key Research and Development Program of China(No.2022YFD1800100) 

主　　题：RABV Inactivated vaccine Incorporation LTB Dendritic cells Activation 

摘      要：Although inactivated vaccines against rabies have the advantage of high safety,effective protection against rabies virus(RABV)infection often requires multiple,high-dose *** a molecular adjuvant into the viral particles has been found to be a useful strategy to promote the immune effectiveness of inactivated *** this study,we constructed a recombinant virus,rCVS11-LTB,which chimerically expresses a molecular adjuvant heat-labile enterotoxin B subunit(LTB)protein on the surface of the RABV *** in vivo was found to be promoted by rCVS11-LTB through the activation of dendritic cells(DCs).Our results demonstrated that inactivated rCVS11-LTB was able to induce higher levels of virusneutralizing antibodies(VNAs)in both mice and dogs than the parent virus rCVS11,to enhance the cellular immune response and T cell immune memory in mice,and was also able to provide 100%protection in mice from lethal doses of rabies virus,indicating its potential as a safe and effective inactivated rabies vaccine candidate.