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Effect of bone marrow-derived monocytes transfected with RNA of mouse colon carcinoma on specific antitumor immunity

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World Journal of Gastroenterology 2005年第5期11卷 760-763页

作者： Xiao-yuanChu long-BangChen JingZang Jing-HuaWang QunZhang huai-ChengGeng DepartmentofOncology NanjingGeneralHospitalofPLANanjing210002JiangsuProvinceChina

AIM: To investigate the effect of bone marrow-derived monocytes transfected with RNA of CT-26 (a cell line of mouse colon carcinoma) on antitumor immunity. METHODS: Mouse bone marrow-derived monocytes were incubated with mouse granulocyte macrophage colony stimulating factor (mGM-CSF) in vitro, and the purity of monocytes was detected by flow cytometry. Total RNA of CT-26 was obtained by TRIzol's process, and monocytes were transfected by TransMessenger in vitro. The activity of cytotoxic T lymphocytes (CTL) in vivo was estimated by the modified lactate dehydrogenase (LDH) release assay. Changes of tumor size in mice and animal's survival time were observed in different groups. RESULTS: Monocytes from mouse bone marrow were successfully incubated, and the positive rate of CDllb was over 95%. Vaccination of the monocytes transfected with total RNA induced a high level of specific CTL activity in vivo, and made mice resistant to the subsequent challenge of parental tumor cells. In vivo effects induced by monocytes transfected with total RNA were stronger than those induced by monocytes pulsed with tumor cell lysates. CONCLUSION: Antigen presenting cells transfected with total RNA of CT-26 can present endogenous? tumor antigens, activate CTL, and effectively induce specific antitumor immunity.

关键词： Colon carcinoma RNA Bone marrow-derived monocytes Cytotoxic T lymphocytes

Evolution of hypersurfaces by the mean curvature minus an external force field

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Science China Mathematics 2007年第2期50卷 231-239页

作者： Yan-nan LIU huai-yu JIAN Department of Mathematical Sciences Tsinghua UniversityBeijing 100084China

In this paper, we study the evolution of hypersurface moving by the mean curvature minus an external force field. It is shown that the flow will blow up in a finite time if the mean curvature of the initial surface is larger than some constant depending on the boundness of derivatives of the external force field. For a linear force, we prove that the convexity of the hypersurface is preserved during the evolution and the flow has a unique smooth solution in any finite time and expands to infinity as the time tends to infinity if the initial curvature is smaller than the slope of the force.

关键词： parabolic equation mean curvature flow maximum principle (for tensor) 35K45 53A05

Roll gap prediction in acceleration and deceleration process of cold rolling based on a data-driven method

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Journal of Iron and Steel Research International 2023年第5期30卷 1013-1021页

作者： yun-jian Hu Jie Sun huai-tao Shi Qing-long Wang Jian-zhao Cao School of Mechanical Engineering Shenyang Jianzhu UniversityShenyang 110168LiaoningChina The State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819LiaoningChina Department of Mechanical Engineering North China Electric Power UniversityBaoding 071003HebeiChina

Severe fluctuation of the effective roll gap in the acceleration and deceleration section of the cold rolling process is a significant factor causing thickness ***,the conventional roll gap compensation method and control strategy do not meet the stringent strip quality *** roll gap model in the acceleration and deceleration process is studied to increase the thickness control *** order to improve model accuracy,a roll gap prediction method based on data-driven is *** the complexities of the cold rolling process,the extreme gradient boosting(XGBoost)method is used to predict the roll gap model as the rolling speed ***,support vector regression and neural network-based methods are taken to evaluate and compare the prediction *** on the field data,the simulation experiments are carried *** demonstrated that the prediction performance of the proposed method outperformed the other two *** values of root mean square error,determination coefficient value,mean absolute percentage error and mean absolute error obtained from the XGBoost model were equal to 0.000346,0.952,7.02,and 0.00028,*** addition,the proposed method analyzed the contribution rates of the rolling affecting parameters on the roll *** data showed that in the controllable rolling parameters,the rolling speed is the most impacting factor that disturbs the roll gap model in the acceleration and deceleration process,which can provide a useful direction for actual roll gap adjustment.

关键词： Cold rolling Acceleration Deceleration Roll gap model Extreme gradient boosting Contribution rate Analysis

基于O'NET半结构化访谈建构大学生职业适应力故事

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高校辅导员学刊 2025年第1期17卷 34-42,97页

作者：林振余权肖龙福建商学院传媒与会展学院福建福州350012

职业适应力是大学生转型为职场人士的关键能力,其中职业兴趣是核心要素。然而,当前对大学生职业兴趣的分析主要局限于定量评估,导致学生对自身职业兴趣的理解较为表面,甚至开始怀疑其价值。研究采用O'NET半结构化访谈,结合主题演绎驱动... 详细信息

职业适应力是大学生转型为职场人士的关键能力,其中职业兴趣是核心要素。然而,当前对大学生职业兴趣的分析主要局限于定量评估,导致学生对自身职业兴趣的理解较为表面,甚至开始怀疑其价值。研究采用O'NET半结构化访谈,结合主题演绎驱动理论,深入分析了大学生在职业适应力故事中的关注、控制、好奇、自信、合作五个维度,旨在探讨半结构化访谈如何定性地将职业兴趣代码与这五个维度的故事关联起来,并揭示其在提升大学生职业适应力方面的作用。研究结果显示,通过半结构化访谈建构的职业适应力故事,成功整合了定量与定性职业兴趣评估,这种方法不仅能帮助大学生更深入地理解自身的霍兰德职业兴趣代码,还能促使他们系统地叙述职业适应力故事,从而有效提升其职业适应力。

关键词：故事建构半结构化访谈职业适应力定性 O'NET

Spatio-temporal variations in organic carbon density and carbon sequestration potential in the topsoil of Hebei Province, China

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Journal of Integrative Agriculture 2016年第11期15卷 2627-2638页

作者： CAO Xiang-hui long huai-yu LEI Qiu-liang LIU Jian ZHANG Ji-zong ZHANG Wen-ju WU Shu-xia Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Pasture Systems and Watershed Management Research Unit USDA-Agricultural Research ServiceUniversity Park

Reliable prediction of soil organic carbon（SOC） density and carbon sequestration potential（CSP） plays an important role in the atmospheric carbon dioxide budget. This study evaluated temporal and spatial variation of topsoil SOC density and CSP of 21 soil groups across Hebei Province, China, using data collected during the second national soil survey in the 1980 s and during the recent soil inventory in 2010. The CSP can be estimated by the method that the saturated SOC content subtracts the actual SOC associated with clay and silt. Overall, the SOC density and CSP of most soil groups increased from the 1980 s to 2010 and varied between different soil groups. Among all soil groups, Haplic phaeozems had the highest SOC density and Endogleyic solonchaks had the largest CSP. Areas of soil groups with the highest SOC density（90 to 120 t C ha^（–1）） and carbon sequestration（120 to 160 t C ha^（–1）） also increased over time. With regard to spatial distribution, the north of the province had higher SOC density but lower CSP than the south. With respect to land-use type, cultivated soils had lower SOC density but higher CSP than uncultivated soils. In addition, SOC density and CSP were influenced by soil physicochemical properties, climate and terrain and were most strongly correlated with soil humic acid concentration. The results suggest that soil groups（uncultivated soils） of higher SOC density have greater risk of carbon dioxide emission and that management should be aimed at maximizing carbon sequestration in soil groups（cultivated soils） with greater CSP. Furthermore, soils should be managed according to their spatial distributions of SOC density and carbon sequestration potential under different soil groups.

关键词： carbon sequestration SOC density spatial variation topsoil

NUMERICAL INVESTIGATION OF SEABED INTERACTION IN TIME DOMAIN ANALYSIS OF MOORING CABLES

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Journal of Hydrodynamics 2006年第4期18卷 424-430页

作者： yu long TAN Jia-hua School of Naval Architecture and Civil Engineering Shanghai Jiaotong University Shanghai 200030 China

In this paper, an efficient two-dimensional finite element model for numerical analysis of mooring cables and seabed interaction has been built. Geometric shape and dynamics of mooring cables are computed in time domain, accounting for the motions of the moored sturcture. In the model, a hybrid beam element is employed to simulate the mooring cable while the seabed is simulated by application of different soil constitutive models. After the elastic and elastic-plastic soil constitutive models have been used for computation, tensions and offsets of mooring cables at fairlead point are also compared accounting for friction effect between cables and seabed. Both transversal and longitudinal behaviors are studied at different water depths.

关键词： dynamics of mooring cable seabed interaction soil constitutive models

Effects of PEG Molecular Weight on Its Interaction with Albumin

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Chinese Journal of Polymer Science 2015年第10期33卷 1373-1379页

作者： Jing-jing Lai huai-yu Yan Yan Liu 黄延宾 Key Laboratory of Advanced Materials(MOE) Department of Chemical Engineering Tsinghua University

The fluorescence property of bovine serum albumin was investigated in the presence of PEGs of different molecular weights. Based on our results, we propose that PEGs of different molecular weights bind to albumin in the pH 7.4 aqueous solution, but only when the polymer size is larger than the protein size, one polymer chain may bind to multiple proteins and cause the proteins to aggregate.

关键词： Polyethylene glycol Bovine serum albumin Protein-polymer interactions Fluorescence analysis

Simultaneous anaerobic-aerobic biodegradation of halogenated phenolic compound under oxygen-limited conditions

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Journal of Environmental Sciences 2005年第5期17卷 873-875页

作者： CHEN yuan-cai LAN Hui-xia ZHAN huai-yu FU Shi-yu State Key Laboratory of Pulp and Paper Engineering South China University of Technology Guangzhou 510641 China Laboratory of Cellulose and Liguocellulosics Chemistry Chinese Academy of Sciences Guangzhou 510650 China

The successful application of co-immobilized aerobic-anaerobic biomass under limited aeration in wastewater treatment systems would eliminate the problems associated with the intermediates mono-chlorophenol（MCP） and di-chlorophenol（DCP） accumulations. With low initial pentachlorophenol（PCP） concentration, all PCP could be completely removed under oxygen-limited strict anaerobic conditions, and the removal efficiencies with different initial headspace oxygen percentage（IHOP） were not obviously different from each other. While at high initial PCP concentration, under strictly anaerobic conditions PCP and their intermediates were clearly higher than that under other conditions, and produced obvious accumulation, the highest PCP reduction was achieved by the system receiving 30 IHOP, oxygen-limited system also exhibited lower residual TOC concentration and lower concentration of metabolic intermediates MCP and DCP. These results suggested that under strictly anaerobic condition the reductive dechlorination of low chlorinated compounds became rate limiting in the reductive dechlorination pathway, less chlorinated compounds be more amenable to aerobic degradation, and the aerobes of outer layers could function under limited oxygen. The co-immobilized aerobic-anaerobic biomass for methanogenesis under limited-aeration for chlorophenol degradation might be an attractive and efficient alternative for the sequential anaerobic/aerobic system to achieve mineralization of a broad range of recalcitrance highly chlorinated organics and low final TOC concentrations.

关键词： PCP oxygen-limited immobilized cellr

Numerical assessment of the erosion risk for cavitating twisted hydrofoil by three methods

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Journal of Hydrodynamics 2021年第4期33卷 698-711页

作者： Ting-ting Lei huai-yu Cheng Bin Ji Xiao-xing Peng State Key Laboratory of Water Resources and Hydropower Engineering Science Wuhan UniversityWuhan430072China National Key Laboratory on Ship Vibration and Noise China Ship Scientific Research CenterWuxi214082China

In this paper, the large eddy simulation (LES) method in conjunction with the Zwart cavitation model is adopted for the assessment of the erosion risk on a hydrofoil surface. The numerical results are in good agreement with the experiments. On this basis, three methods, namely the intensity function method (IFM), the time-averaged aggressiveness indicators (TAIs) and the gray level method (GLM), are applied for the assessment of the erosion risk. It is shown that the erosion intensity index of the IFM is extremely sensitive to the artificially selected thresholds, which greatly limits the application of the method. The erosion risk predicted by four indicators in the TAIs does not agree well with the experimental results. Further analysis demonstrates that the GLM using the instantaneous pressure field is relatively satisfactory, which can provide a reasonable assessment of the erosion and is not very sensitive to the artificially selected thresholds. To further improve the accuracy of the GLM for the erosion risk prediction, the time-average pressure field is adopted in the GLM for the erosion evaluation. It is suggested that the erosion assessment by using the time-averaged pressure field is in better agreement with the experimental results when compared with that by using the instantaneous pressure field.

关键词： Cavitating flow erosion numerical simulation threshold selection