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Journal of Pharmaceutical Analysis 2022年第5期12卷 801-807页

作者： Xin-Yuan Hu Jia-Jing Li Zi-Wei Yang Jun Zhang huai-song wang Department of Pharmaceutical Analysis China Pharmaceutical UniversityNanjing210009China College of Food Science and Bioengineering Tianjin Agricultural UniversityTianjin300392China

The catalytic generation of H_(2) in living cells provides a method for antioxidant therapy.In this study,an[FeFe]-hydrogenase mimic[Ru+Fe_(2)S_(2)@F127(80)]was synthesized by self-assembling polymeric pluronic F-127,catalytic[Fe_(2)S_(2)]sites,and photosensitizer Ru(bpy)_(3)^(2+).Under blue light irradiation,hydrated protons were photochemically reduced to H2,which increased the local pH in living cells(HeLa cells).The generated H2 was subsequently used as an antioxidant to decrease reactive oxygen species(ROS)levels in living cells(HEK 293T,HepG2,MCF-7,and HeLa cells).Our findings revealed that the proliferation of HEK 293T cells increased by a factor of about six times,relative to that of other cells(HepG2,MCF-7,and HeLa cells).Intracellular ROS and pH levels were then monitored using fluorescent cell imaging.Our study showed that cell imaging can be used to evaluate the ability of Ru t Fe2S2@F127 to eliminate oxidative stress and prevent ROS-related diseases.

关键词： Cell imaging [FeFe]-hydrogenase Reactive oxygen species Photocatalytic system

Hapln1 promotes dedifferentiation and proliferation of iPSC-derived cardiomyocytes by promoting versican-based GDF11 trapping

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Journal of Pharmaceutical Analysis 2024年第3期14卷 335-347页

作者： Ding-Jun Hao Yue Qin Shi-Jie Zhou Bu-huai Dong Jun-song Yang Peng Zou Li-Ping wang Yuan-Ting Zhao Department of Spine Surgery Honghui HospitalXi'an Jiaotong UniversityXi'an710054China Department of Anesthesiology Honghui HospitalXi'an Jiaotong UniversityXi'an710054China Department of Cardiovascular Medicine Honghui HospitalXi'an Jiaotong UniversityXi'an710054China State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy West China HospitalSichuan UniversityChengdu610041China

Hyaluronan and proteoglycan link protein 1(Hapln1)supports active cardiomyogenesis in zebrafish hearts,but its regulation in mammal cardiomyocytes is unclear.This study aimed to explore the potential regulation of Hapln1 in the dedifferentiation and proliferation of cardiomyocytes and its therapeutic value in myocardial infarction with human induced pluripotent stem cell(hiPSC)-derived cardiomyocytes(CMs)and an adult mouse model of myocardial infarction.HiPSC-CMs and adult mice with myocardial infarction were used as in vitro and in vivo models,respectively.Previous single-cell RNA sequencing data were retrieved for bioinformatic exploration.The results showed that recombinant human Hapln1(rhHapln1)promotes the proliferation of hiPSC-CMs in a dose-dependent manner.As a physical binding protein of Hapln1,versican interacted with Nodal growth differentiation factor(NODAL)and growth differentiation factor 11(GDF11).GDF11,but not NODAL,was expressed by hiPSC-CMs.GDF11 expression was unaffected by rhHapln1 treatment.However,this molecule was required for rhHapln1-mediated activation of the transforming growth factor(TGF)-β/Drosophila mothers against decapentaplegic protein(SMAD)2/3 signaling in hiPSC-CMs,which stimulates cell dedifferentiation and proliferation.Recombinant mouse Hapln1(rmHapln1)could induce cardiac regeneration in the adult mouse model of myocardial infarction.In addition,rmHapln1 induced hiPSC-CM proliferation.In conclusion,Hapln1 can stimulate the dedifferentiation and proliferation of iPSC-derived cardiomyocytes by promoting versican-based GDF11 trapping and subsequent activation of the TGF-β/SMAD2/3 signaling pathway.Hapln1 might be an effective hiPSC-CM dedifferentiation and proliferation agent and a potential reagent for repairing damaged hearts.

关键词： Hapln1 Versican GDF11 iPSC-CMs Cardiomyocyte proliferation

A three-dimensional co-continuous network structure polymer electrolyte with efficient ion transport channels enabling ultralong-life all solid-state lithium metal batteries

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Journal of Energy Chemistry 2024年第7期94卷 635-645页

作者： Meng wang Hu Zhang Yewen Li Ruiping Liu huai Yang School of Chemical&Environmental Engineering China University of Mining and Technology(Beijing)Beijing 100083China Beijing Advanced Innovation Center for Materials Genome Engineering&School of Materials Science and Engineering Peking UniversityBeijing 100871China

Solid polymer electrolytes(SPEs)have emerged as one of the most promising candidates for the construction of solid-state lithium batteries due to their excellent flexibility,scalability,and interface compatibility with electrodes.Herein,a novel all-solid polymer electrolyte(PPLCE)was fabricated by the copolymer network of liquid crystalline monomers and poly(ethylene glycol)dimethacrylate(PEGDMA)acts as a structural frame,combined with poly(ethylene glycol)diglycidyl ether short chain interspersed serving as mobile ion transport entities.The preparaed PPLCEs exhibit excellent mechanical property and out-standing electrochemical performances,which is attributed to their unique three-dimensional cocontinuous structure,characterized by a cross-linked semi-interpenetrating network and an ionic liquid phase,resulting in a distinctive nanostructure with short-range order and long-range disorder.Remarkably,the addition of PEGDMA is proved to be critical to the comprehensive performance of the PPLCEs,which effectively modulates the microscopic morphology of polymer networks and improves the mechanical properties as well as cycling stability of the solid electrolyte.When used in a lithiumion symmetrical battery configuration,the 6 wt%-PPLCE exhibites super stability,sustaining operation for over 2000 h at 30 C,with minimal and consistent overpotential of 50 mV.The resulting Li|PPLCE|LFP solid-state battery demonstrates high discharge specific capacities of 160.9 and 120.1 mA h g^(-1)at current densities of 0.2 and 1 C,respectively.Even after more than 300 cycles at a current density of 0.2 C,it retaines an impressive 73.5%capacity.Moreover,it displayes stable cycling for over 180 cycles at a high current density of 0.5C.The super cycle stability may promote the application for ultralong-life all solid-state lithium metal batteries.

关键词： Solid-state electrolyte Lithium-metal batteries Liquid crystalline polymer Copolymer 3D co-continuous structure Long cycle stability

Mechanical properties and thermal deformation behavior of low-cost titanium matrix composites prepared by a structure-optimized Y_(2)O_(3) crucible

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Journal of Iron and Steel Research(International) 2024年第3期31卷 738-751页

作者： Qian Dang Gang Huang Ye wang Chi Zhang Guo-huai Liu Zhao-dong wang The State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819LiaoningChina School of Materials Science and Chemical Engineering Harbin University of Science and TechnologyHarbin 150004HeilongjiangChina School of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870LiaoningChina

A porous yttrium oxide crucible with both thermal shock resistance and erosion resistance was developed by structural optimization.The structure-optimized yttrium oxide crucible was proved to be suitable for melting highly reactive titanium alloys.Low-cost(TiB+Y2O_(3))-reinforced titanium matrix composites were prepared by vacuum induction melting using the prepared crucible.The thermal deformation behavior and microstructure evolution of(TiB+Y2O_(3))-reinforced tita-nium matrix composites were investigated at deformation temperatures of 900-1100℃with strain rates of 0.001-1 s-1.The results showed that the prepared yttrium oxide crucible had both thermal shock and erosion resistance,the low-cost titanium matrix composites could be prepared by the developed yttrium oxide crucibles which were homogeneous in composition and highly sensitive to strain rate and deformation temperature,and the peak and theological stresses decreased with increasing deformation temperature or decreasing strain rate.In addition,the average thermal deformation activation energy of the composites was calculated to be 574.6 kJ/mol by establishing the Arrhenius constitutive equation in consideration of the strain variables,and the fitting goodness between the predicted stress value and the measured value was 97.624%.The calculated analysis of the hot processing map showed that the best stable thermal deformation zone was located in the deformation temperature range of 1000-1100℃and strain rate range of 0.001-0.01 s^(-1),where the peak dissipation coefficient wasη=71%.In this zone,the deformation of the reinforcement and matrix was harmonious,the reinforcement was less likely to fracture,dynamic recrystallization occurred more fully and the alloy exhibited near steady rheological characteristics.

关键词： Yttrium oxide crucible Titanium matrix composite Thermal deformation behavior Constitutive equation Hot processing map Microstructure evolution

5-Hydroxytryptamine-induced proliferation of pulmonary artery smooth muscle cells are extracellular signal-regulated kinase pathway dependent

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Acta Pharmacologica Sinica 2005年第5期26卷 563-567页

作者： Dan song,huai-liang wang~2,Shuang wang,Xin-hua ZHANGDepartment of Clinical Pharmacology,China Medical University,Shenyang 110001,China Department of Clinical Pharmacology China Medical University Shenyang China

Aim:To investigate the effect of 5-hydroxytryptamine transporter(5-HTT)inhibi-tor fluoxetine and antisense oligodeoxynucleotide(ODN)to extracelluar signal-regulated kinases(ERKs)on pulmonary arterial smooth muscle cells(PASMCs)proliferation induced by 5-HT.Methods:Liposomal transfection was used tointroduce ODNs to ERK1/2 into cultured rat PASMCs and the transfection effi-ciency was measured by observing the uptake of the fluorecein isothiocynate(FITC)-labeled antisense ODN in PASMCs.The effects of 5-HTT selective inhibi-tor fluoxetine and ODNs on the proliferation of PASMCs were evaluated by cellnumber counting and cell cycle analysis,and measured by microculture tetrazo-lium(MTT)assay and flow cytometry(FCM),respectively.Results:Liposomesmediated the transfection of ODNs into PASMCs with high efficiency.MTTassay showed fluoxetine(10μmol/L,1 μmol/L,and 100 nmol/L)concentrationdependently inhibited the proliferation of PASMCs induced by 5-HT(1 μmol/L)invitro.The proliferation rate of PASMCs by 5-HT was significantly inhibited bypretreatment with ERK1/2 antisense ODN(0.2μmol/L)from 251%±18% to 86%±5%(P<0.01).Flow cytometric analysis of cell cycle distribution showed that theincrease of 5-HT induced S phase fraction(SPF)and proliferation index(PI)weresignificantly inhibited by fluoxetine(1μmol/L)or antisense ODN with SPF from36%±4% to 26%±3% and 24%±4%,and PI from 34%±2% to 29%±2% and 24%±2%,respectively.Conclusion:5-HTT mediates the mitogenic effect of 5-HT onPASMCs and the proliferation of PASMCs induced by 5-HT is dependent onERKs signal pathway.

关键词： antisense oligodeoxyribonucleotides serotonin membrane transport proteins pulmonary hypertension vascular smooth muscle fluxetine mitogen-activated protein kinases

Arbuscular mycorrhizal fungi reduce ammonia emissions under different land-use types in agro-pastoral areas

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Pedosphere 2024年第2期34卷 497-507页

作者： huaisong wang Rui GUO Yibo TIAN Nan CUI Xinxin wang Lei wang Zhongbao YANG Shuying LI Jixun GUO Lianxuan SHI Tao ZHANG Institute of Grassland Science Northeast Normal UniversityKey Laboratory of Vegetation EcologyMinistry of EducationJilin Songnen Grassland Ecosystem National Observation and Research StationChangchun 130024China Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural SciencesKey Laboratory of Dryland AgricultureMinistry of AgricultureBeijing 100081China Mountainous Area Research Institute of Hebei Province Hebei Agricultural UniversityBaoding 071001China College of Agronomy Liaocheng UniversityLiaocheng 252059China The Key Laboratory of Plant Development and Environmental Adaptation Biology Ministry of EducationSchool of Life ScienceShandong UniversityQingdao 266237China Forestry and Grassland Bureau of Aohan Banner Chifeng 024005China

Ammonia(NH3)emissions,the most important nitrogen(N)loss form,always induce a series of environmental problems such as increased frequency of regional haze pollution,accelerated N deposition,and N eutrophication.Arbuscular mycorrhizal(AM)fungi play key roles in N cycling.However,it is still unclear whether AM fungi can alleviate N losses by reducing NH3emissions.The potential mechanisms by which AM fungi reduce NH_(3)emissions in five land-use types(grazed grassland,mowed grassland,fenced grassland,artificial alfalfa grassland,and cropland)were explored in this study.Results showed that AM fungal inoculation significantly reduced NH3emissions,and the mycorrhizal responses of NH3emissions were determined by land-use type.Structural equation modeling(SEM)showed that AM fungi and land-use type directly affected NH_(3)emissions.In addition,the reduction in NH_(3)emissions was largely driven by the decline in soil NH_(4)^(+)-N and pH and the increases in abundances of ammonia-oxidizing archaea(AOA)amoA and bacteria(AOB)amoB genes,urease activity,and plant N uptake induced by AM fungal inoculation and land-use type.The present results highlight that reducing the negative influence of agricultural intensification caused by land-use type changes on AM fungi should be considered to reduce N losses in agriculture and grassland ecosystems.

关键词： agricultural intensification grassland management functional genes plant N uptake N leaching N loss

Three-dimensional choroidal vascularity index and choroidal thickness in fellow eyes of acute and chronic primary angle-closure using swept-source optical coherence tomography

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International Journal of Ophthalmology(English edition) 2024年第1期17卷 42-52页

作者： Hai-Li Huang Guan-Hong wang Liang-Liang Niu Xing-huai Sun Department of Ophthalmology&Visual Science Eye&ENT HospitalShanghai Medical CollegeFudan UniversityShanghai 200031China State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain ScienceFudan UniversityShanghai 200032China NHC Key Laboratory of Myopia Chinese Academy of Medical Sciencesand Shanghai Key Laboratory of Visual Impairment and Restoration(Fudan University)Shanghai 200031China

AIM:To compare the three-dimensional choroidal vascularity index(CVI)and choroidal thickness between fellow eyes of acute primary angle-closure(F-APAC)and chronic primary angle-closure glaucoma(F-CPACG)and the eyes of normal controls.METHODS:This study included 37 patients with unilateral APAC,37 with asymmetric CPACG without prior treatment,and 36 healthy participants.Using swept-source optical coherence tomography(SS-OCT),the macular and peripapillary choroidal thickness and three-dimensional CVI were measured and compared globally and sectorally.Pearson’s correlation analysis and multivariate regression models were used to evaluate choroidal thickness or CVI with related factors.RESULTS:The mean subfoveal CVIs were 0.35±0.10,0.33±0.09,and 0.29±0.04,and the mean subfoveal choroidal thickness were 315.62±52.92,306.22±59.29,and 262.69±45.55μm in the F-APAC,F-CPACG,and normal groups,respectively.All macular sectors showed significantly higher CVIs and choroidal thickness in the F-APAC and F-CPACG eyes than in the normal eyes(P<0.05),while there were no significant differences between the F-APAC and F-CPACG eyes.In the peripapillary region,the mean overall CVIs were 0.21±0.08,0.20±0.08,and 0.19±0.05,and the mean overall choroidal thickness were 180.45±54.18,174.82±50.67,and 176.18±37.94μm in the F-APAC,F-CPACG,and normal groups,respectively.There were no significant differences between any of the two groups in all peripapillary sectors.Younger age,shorter axial length,and the F-APAC or F-CPACG diagnosis were significantly associated with higher subfoveal CVI and thicker subfoveal choroidal thickness(P<0.05).CONCLUSION:The fellow eyes of unilateral APAC or asymmetric CPACG have higher macular CVI and choroidal thickness than those of the normal controls.Neither CVI nor choroidal thickness can distinguish between eyes predisposed to APAC or CPACG.A thicker choroid with a higher vascular volume may play a role in the pathogenesis of primary angle-closure glaucoma.

关键词： choroidal thickness choroidal vascularity index swept-source optical coherence tomography acute primary angle-closure chronic primary angle-closure glaucoma

Functional Optical Fiber Sensors Detecting Imperceptible Physical/Chemical Changes for Smart Batteries

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Nano-Micro Letters 2024年第8期16卷 268-308页

作者： Yiding Li Li wang Youzhi song Wenwei wang Cheng Lin Xiangming He Institute of Nuclear and New Energy Technology Tsinghua UniversityBeijing 100084People’s Republic of China National Engineering Research Center of Electric Vehicles Beijing Institute of Technology(BIT)Beijing 100081People’s Republic of China Shenzhen Automotive Research Institute of BIT(Shenzhen Research Institute of National Engineering Research Center of Electric Vehicles) Shenzhen 518118People’s Republic of China

The battery technology progress has been a contradictory process in which performance improvement and hidden risks coexist.Now the battery is still a“black box”,thus requiring a deep understanding of its internal state.The battery should“sense its internal physical/chemical conditions”,which puts strict requirements on embedded sensing parts.This paper summarizes the application of advanced optical fiber sensors in lithium-ion batteries and energy storage technologies that may be mass deployed,focuses on the insights of advanced optical fiber sensors into the processes of one-dimensional nano-micro-level battery material structural phase transition,electrolyte degradation,electrode-electrolyte interface dynamics to three-dimensional macro-safety evolution.The paper contributes to understanding how to use optical fiber sensors to achieve“real”and“embedded”monitoring.Through the inherent advantages of the advanced optical fiber sensor,it helps clarify the battery internal state and reaction mechanism,aiding in the establishment of more detailed models.These advancements can promote the development of smart batteries,with significant importance lying in essentially promoting the improvement of system consistency.Furthermore,with the help of smart batteries in the future,the importance of consistency can be weakened or even eliminated.The application of advanced optical fiber sensors helps comprehensively improve the battery quality,reliability,and life.

关键词： Smart battery Advanced embedded optical fiber sensor Battery internal physical/chemical state Quality-reliability-life characteristic

Practical Prescribed Time Tracking Control With Bounded Time-Varying Gain Under Non-Vanishing Uncertainties

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IEEE/CAA Journal of Automatica Sinica 2024年第1期11卷 219-230页

作者： Dahui Luo Yujuan wang Yongduan song the Key Laboratory of Dependable Service Computing in Cyber Physical Society Ministry of EducationChongqing UniversityChongqing 400044 the School of Automation Chongqing UniversityChongqing 400044 the Star Institute for Intelligent Systems Chongqing 400044 IEEE

This paper investigates the prescribed-time control(PTC) problem for a class of strict-feedback systems subject to non-vanishing uncertainties. The coexistence of mismatched uncertainties and non-vanishing disturbances makes PTC synthesis nontrivial. In this work, a control method that does not involve infinite time-varying gain is proposed, leading to a practical and global prescribed time tracking control solution for the strict-feedback systems, in spite of both the mismatched and nonvanishing uncertainties. Different from methods based on control switching to avoid the issue of infinite control gain that involves control discontinuity at the switching point, in our method a softening unit is exclusively included to ensure the continuity of the control action. Furthermore, in contrast to most existing prescribed-time control works where the control scheme is only valid on a finite time interval, in this work, the proposed control scheme is valid on the entire time interval. In addition, the prior information on the upper or lower bound of gi is not in need,enlarging the applicability of the proposed method. Both the theoretical analysis and numerical simulation confirm the effectiveness of the proposed control algorithm.

关键词： Adaptive control prescribed time control(PTC) strict-feedback systems tracking control

Low profile cavity-embedded ultra-wideband UHF array antenna with end-fire beams

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Journal of Electronic Science and Technology 2024年第2期22卷 24-35页

作者： Huan He Wen-song wang Southwest China Institute of Electronic Technology Chengdu610036China School of Electrical and Electronic Engineering Department Nanyang Technological UniversitySingapore639798Singapore

A low-profile,vertically polarized,ultra-wideband array antenna with end-fire beams operating in an ultra-high frequency(UHF)band is developed in this paper.The array antenna consists of 1×16 log-periodic top-hat loaded monopole antenna arrays and is feasible to embed into a shallow cavity to further reduce the array height.Capacitance is introduced in the proposed antenna element to reduce profile height and the rectangular top hats are carefully designed to minimize the transverse dimension.Simulated results show that when the antenna array operates in a frequency range of 300 MHz-900 MHz,the end-fire radiation pattern achieves±45°scanning range in the horizontal plane.Then prototypes of the proposed end-fire antenna element and a uniformly spaced linear array(1×2)are fabricated and validated.The end-fire antenna array should be suitable for airborne applications where low-profile and conformal scanning phased antenna arrays with end-fire radiations are required.This design is attractive for airborne platform applications that are used to search,discover,identify,and scout the aerial target with vertically polarized beams.

关键词： End-fire radiation Low-profile Vertical polarization Log-periodic array