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Virologica Sinica 2014年第5期29卷 291-298页

作者： Aiguo Xin Mingwang Zhu Qi Hu Haisheng Miao Zhenqi Peng Yuwen He lin Gao huachun li Yunnan Tropical and Subtropical Animal Virus Disease Laboratory Yunnan Animal Science and Veterinary Institute Yunnan Provincial Research Center for Veterinary Biological Products

An amino acid mutation(R127→I) in the 3A non-structural protein of an FMDV serotype Asia1 rabbit-attenuated ZB strain was previously found after attenuation of the virus. To explore the effects of this mutation on viral replication and infection, the amino acid residue isoleucine(I) was changed to arginine(R) in the infectious cDNA clone of the rabbit-attenuated ZB strain by sitedirected mutagenesis, and the R127-mutated virus was rescued. BHK monolayer cells and suckling mice were inoculated with the R127-mutated virus to test its growth property and pathogenicity, respectively. The effects of the R127 mutation on viral replication and virulence were analyzed. The data showed that there was a slight difference in plaque morphology between the R127-mutated and wild-type viruses. The growth rate of the mutated virus was lower in BHK-21 cells and its virulence in suckling mice was also attenuated. This study indicates that the R127 mutation in 3A may play an important role in FMDV replication in vitro and in pathogenicity in suckling mice.

关键词： foot-and-mouth disease virus(FMDV) 3A protein mutation replication ability virulence

WRF Numerical Simulation Analysis of Meteorological Elements in the Main Urban Area of Chongqing from 2013 to 2015

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Meteorological and Environmental Research 2022年第4期13卷 59-61,66页

作者： Rui YUAN Zhenliang li Dan ZHANG Wei HU huachun li Chongqing Academy of Ecological Environmental Sciences Chongqing 401147China Southwest Branch of Chinese Research Academy of Environmental Sciences Chongqing 401147China Wanzhou District Ecological Environment Monitoring Station Chongqing 404100China

Using the WRF mesoscale numerical model,based on the FNL global reanalysis data of NCEP,numerical simulations with a resolution of 27,9 and 3 km were carried out on the meteorological elements in four national air quality control stations(Jinyun Mountain,Shapingba,Jiefangbei and Nanping)in the main urban area of Chongqing City in July from 2013 to *** results show that at the concerned points,there was an obvious southeast dominant wind at 850 *** meteorological conditions in July in the main urban area of Chongqing were favorable for the diffusion of *** is,the height of the mixed layer was higher,and the precipitation was higher;the temperature and wind speed at 850 hPa were *** with 2013 and 2014,the meteorological conditions in July 2015 were not more favorable for the diffusion of pollutants.

关键词： WRF Numerical simulation Meteorological conditions Chongqing City

Numerical Simulation of Distribution of VOCs Concentration in the Main Urban Area of Chongqing Based on CALPUFF

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Meteorological and Environmental Research 2022年第4期13卷 14-18,24页

作者： Pingjiang LV Zhenliang li Min CHEN Dan ZHANG huachun li Chongqing Academy of Ecological Environmental Sciences Chongqing 401147China Southwest Branch of Chinese Research Academy of Environmental Sciences Chongqing 401147China Wanzhou District Ecological Environment Monitoring Station Chongqing 404100China

The distribution of VOCs concentration in the main urban area of Chongqing was simulated based on the CALPUFF *** results show that the long-term contribution of key industrial pollution sources of VOCs in the study area was not significant(0-0.13 mg/m^(3)),and the hourly average instantaneous contribution was significant(the maximum was up to 2.75 mg/m^(3));the hourly average concentration of VOCs in the area where the sensitive points were located rose abruptly at different times(with a maximum of 1.35 mg/m^(3)).The distribution of VOCs concentration in this area was influenced by the dominant wind,and the area where the sensitive points were located was not the area covered by the maximum landing concentration of *** the dominant wind changed,the pollution situation of the sensitive points may be worsen.

关键词： CALPUFF model VOCs Spatial distribution Numerical simulation

Effect of Viral Antigen Levels on the Serological Response and Efficiency of the Binary Ethylenimine-Inactivated Bluetongue Virus Serotype-16 Vaccine

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World Journal of Vaccines 2016年第4期6卷 47-63页

作者： Le li Haisheng Miao Defang liao Meiling Kou lin Gao huachun li Yunnan Veterinary and Animal Science Institute Kunming China

Bluetongue (BT) is a serious hemorrhagic disease of ruminants caused by bluetongue virus (BTV). Inactive BTV vaccines have been successful in field trials in some areas, and inactivated vaccines are considered safer. However, information about the effect of the viral antigen level on the serological response and efficiency of the inactive BTV-16 vaccine is lacking. In the present study, the serological response and efficiency of the viral antigen concentration in the binary ethylenimine-inactivated Chinese BTV serotype-16 vaccine were investigated. The viral antigens in the viral suspension (VS) were quantified using a modified BTV AC-EliSA method. Four batches of vaccine containing 1, 5, 10, and 50 μg/ml of viral antigen were generated from the VS. Four groups of naive Chinese sheep were vaccinated with the different vaccine batches, and the serological response and vaccine efficiency were investigated before and after challenge infection. The vaccines containing 10 and 50 μg/ml of viral antigen induced significant EliSA and neutralizing antibody titers 14 days after vaccination, whereas the vaccines containing 1 and 5 μg/ml of viral antigen did not have these effects. A booster immunization at 21 days enhanced all groups’ antibody titers;however, the increased titer was related to the viral antigen level. In contrast to the serological response, the viral antigen level of the vaccines did not have a significant effect on the vaccine efficiency. With the exception of one sheep from the 5 μg/ml viral antigen group, all vaccinated sheep from the four antigen level groups showed strong resistance to infection based on their clinical symptoms, rectal temperatures and viremia. Collectively, these data suggested that viral antigen levels from 1 to 50 μg/ml had a significant effect on the serological response of the animals but a limited effect on the vaccine efficiency. The BTV-16 vaccine containing 1 μg/ml of viral antigen was sufficient to achieve high efficiency, but

关键词： Blue-Tongue Virus Serotype-16 Binary Ethylenimine-Inactivated Vaccine Viral Antigen Level Antibody Efficiency

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液力式惯容器管道阻尼设计研究

振动与冲击 2024年第18期43卷 106-112页

作者：李鑫刘骁夫李华春中国农业大学工学院北京100083

针对液力式惯容器相互耦合的惯容管道阻尼、惯容量和有效刚度等特性极大限制了其振动控制范围的问题,提出了一种全新蜿蜒式布局的惯容管道。基于液力式惯容器的工作机理,利用机械与液压网络类比法建立了蜿蜒式管道对应阻尼的数学模型,... 详细信息

针对液力式惯容器相互耦合的惯容管道阻尼、惯容量和有效刚度等特性极大限制了其振动控制范围的问题,提出了一种全新蜿蜒式布局的惯容管道。基于液力式惯容器的工作机理,利用机械与液压网络类比法建立了蜿蜒式管道对应阻尼的数学模型,仿真验证了在惯容量和有效刚度恒定时,通过调节蜿蜒式管道的关键设计参数可实现一定程度的管道阻尼独立调节。此外,研制了蜿蜒式管道的液力式惯容器原型样机,试验验证了蜿蜒式管道阻尼的模型正确性以及原型样机的性能准确性。结果表明,蜿蜒式管道设计实现了管道阻尼与惯容量、有效刚度的物理解耦,拓宽了液力式惯容器的振动控制范围,为同类液力式减振设备的研发提供了一种切实可行的阻尼设计新思路。

关键词：液力式惯容器管道阻尼液压结构设计振动控制动力学测试

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应用有限元方法优化应力锥设计

高电压技术 2005年第11期31卷 55-57页

作者：李华春章鹿华周作春北京电力公司北京100031 华北电力大学北京102206

采用有限元法设计全预制式高压电缆户外的终端电场,通过调整应力锥曲线优化了终端电场分布,即分别选取不同的应力锥长度和曲率来计算和筛选电场应力分布最优的设计尺寸。计算表明,应力锥长130 mm、端部曲率半径20 mm时各项性能指标完全... 详细信息

采用有限元法设计全预制式高压电缆户外的终端电场,通过调整应力锥曲线优化了终端电场分布,即分别选取不同的应力锥长度和曲率来计算和筛选电场应力分布最优的设计尺寸。计算表明,应力锥长130 mm、端部曲率半径20 mm时各项性能指标完全满足要求。对比常规计算方法,该方法能准确计算出终端电场分布,优化应力锥设计。

关键词：有限元优化设计应力锥

硅钨酸与盐酸巴马汀相互作用光谱研究及其分析应用

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化学学报 2012年第6期70卷 747-752页

作者：李华春覃明丽谭克俊发光与实时分析教育部重点实验室西南大学化学化工学院重庆400715 南充农产品质量监测检验中心南充637000

在pH为0.91的HCl-NaOAc缓冲介质中,盐酸巴马汀与硅钨酸通过静电相互作用导致共振光散射信号显著增强,最大散射波长为297 nm,增强的散射信号强度与盐酸巴马汀浓度在0.09～3.2μg/mL范围内呈线性关系,据此建立了盐酸巴马汀的共振光散射分... 详细信息

在pH为0.91的HCl-NaOAc缓冲介质中,盐酸巴马汀与硅钨酸通过静电相互作用导致共振光散射信号显著增强,最大散射波长为297 nm,增强的散射信号强度与盐酸巴马汀浓度在0.09～3.2μg/mL范围内呈线性关系,据此建立了盐酸巴马汀的共振光散射分析方法,检测限为9.1 ng/mL.实验考察了pH、离子强度对体系的影响,研究了体系紫外吸收光谱及荧光光谱,讨论了共振光散射增强机理.该方法用于黄藤素片、黄藤素胶囊及血清样品中盐酸巴马汀含量测定,RSD≤4.2%.

关键词：共振光散射盐酸巴马汀硅钨酸

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同相两根并联大截面交联电缆敷设方式的探讨

高电压技术 2005年第11期31卷 66-67页

作者：韩晓鹏李华春周作春北京电力公司北京100041

介绍了北京市部分地下、半地下变电所10 kV交联聚乙烯绝缘1 600 mm2单芯所内同相两根并联电缆的敷设、运行情况。通过计算分析出其载流量不平衡的主要原因是同相两根并联电缆的外感值不等。建议在同相两根并联10 kV大截面电缆线路中采... 详细信息

介绍了北京市部分地下、半地下变电所10 kV交联聚乙烯绝缘1 600 mm2单芯所内同相两根并联电缆的敷设、运行情况。通过计算分析出其载流量不平衡的主要原因是同相两根并联电缆的外感值不等。建议在同相两根并联10 kV大截面电缆线路中采用上下两层水平布置和三角形排列布置的敷设方式。

关键词：单芯电缆阻抗同相两根并联电缆敷设方式

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电力电缆状态在线监测系统的设计及应用

高电压技术 2019年第11期45卷 3459-3466页

作者：郭卫周松霖王立裴欢张成李华春国网北京市电力公司电力科学研究院北京100075 国网北京市电力公司亦庄供电公司北京100041 中国能源建设集团广东省电力设计研究院有限公司广州510700 国网北京市电力公司检修分公司北京100075 国网北京市电力公司北京100041

高压电缆节约用地、可靠性高,在我国大城市中采用电缆线路取代传统的架空线路是必然趋势。为此基于城市电缆建设的发展需要,着重研究了电力电缆状态在线监测系统。各子系统在设计时,充分结合具体工况环境和经济性指标,选取配套的传感器... 详细信息

高压电缆节约用地、可靠性高,在我国大城市中采用电缆线路取代传统的架空线路是必然趋势。为此基于城市电缆建设的发展需要,着重研究了电力电缆状态在线监测系统。各子系统在设计时,充分结合具体工况环境和经济性指标,选取配套的传感器设备和数据采集系统等监测单元,其工作供电利用低压远程供电和载波通讯信号同芯线对共缆传输技术,可实现不间断地将采集到的各状态参量通过TCP/IP有线网络+数字编址式载波通信网络+现场总线网络一体化网络通讯系统上传到监测主机,最终实现电缆护层接地电流在线监测;电缆接头红外矩阵测温在线监测;以及电缆局部放电在线监测和故障定位。结合在北京500 kV电缆工程实际应用情况,分析了在线监测系统可实现的核心功能,为相关单位开展类似项目的建设提供工程借鉴。

关键词：高压电缆护层接地电流电缆接头测温局部放电监测电缆状态在线监测系统

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电力电缆线路运行温度在线检测技术应用研究

高电压技术 2007年第1期33卷 169-172页

作者：罗俊华周作春李华春罗旻国网武汉高压研究院武汉430074 北京电力公司北京100031 华中科技大学电气与电子工程学院武汉430074

电力电缆线路运行温度在线检测技术为当前及时发现电力电缆线路的局部过热点位置、检测运行线路的绝缘状态、计算导体载流量的首选技术措施,为此基于光频域反射OFDR测温法和TCP/IP通讯协议,提出分布式光纤连续在线检测长距离电力电缆线... 详细信息

电力电缆线路运行温度在线检测技术为当前及时发现电力电缆线路的局部过热点位置、检测运行线路的绝缘状态、计算导体载流量的首选技术措施,为此基于光频域反射OFDR测温法和TCP/IP通讯协议,提出分布式光纤连续在线检测长距离电力电缆线路表面温度方法和技术,有效地克服了基于光时域反射OTDR测温法的光纤测温技术中存在的不足,提高了检测温度的速度。研究结果表明,基于OFDR测温技术的分布式光纤电力电缆运行温度在线检测装置基本满足≥110kV电压的电力电缆线路运行温度在线连续检测的技术要求。

关键词：电力电缆运行温度光纤测温在线检测应用研究