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ISSR-Based Molecular Characterization of an Elite Germplasm Collection of Sweet Potato(Ipomoea batatas L.) in China

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Journal of Integrative Agriculture 2014年第11期13卷 2346-2361页

作者： zhang Kai WU Zheng-dan li yan-hua zhang Han WANG liang-ping zhou Quan-lu TANG Dao-bin FU Yu-fan HE Feng-fa jiang Yu-chun yang Hang WANG Ji-chun College of Agronomy and Biotechnology Southwest University Sweet Potato Engineering and Technology Research Center Engineering Research Center of South Upland Agriculture Ministry of Education The Chongqing Three Gorges Academy of Agricultural Sciences Nanchong Institute of Agricultural Sciences College of life Science Southwest University

To determine the genetic diversity and population structure of sweet potato accessions cultivated in China, and to establish the genetic relationships among their germplasm types, a representative collection of 240 accessions was analyzed using inter-simple sequence repeat （ISSR） markers. The mean genetic similarity coefifcient, Nei’s gene diversity, and shared allele distance of tested sweet potato accessions were 0.7302, 0.3167 and 0.2698, respectively. The 240 accessions could be divided into six subgroups and ifve subpopulations based on neighbor-joining （NJ） clustering and STRUCTURE results, and obvious genetic relationships among the tested sweet potato accessions were identiifed. The marker-based NJ clustering and population structure showed no distinct assignment pattern corresponding to lfesh color or geographical ecotype of the tested sweet potato germplasm. Analysis of molecular variance （AMOVA） revealed small but signiifcant difference between white and orange-lfeshed sweet potato accessions. Small but signiifcant difference were also observed among sweet potato accessions from the Southern summer-autumn sweet potato region, the Yellow River Basin spring and summer sweet potato region and the yangtze River Basin summer sweet potato region. This study demonstrates that genetic diversity in the tested sweet potato germplasm collection in China is lower than that in some reported sweet potato germplasm collections from other regions. Pedigree investigations suggest that more diverse Chinese sweet potato varieties should be formed by broadening the selection scope of breeding parents and incorporating the introduced varieties into future breeding programs.

关键词： sweet potato genetic diversity population structure agro-ecological zone lfesh color ISSR

Ultrasensitive and accurate diagnosis of urothelial cancer by plasmonic AuNRs-enhanced fluorescence of near-infrared Ag_(2)S quantum dots

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Rare Metals 2022年第11期41卷 3828-3838页

作者： Dong zhang Cai-Ping Ding Xiao-Yue Zheng Jia-zhou Ye Zi-Hai Chen Jian-hua li Ze-Jun yan Jun-Hui jiang You-Ju huang Department of Urology&Nephrology Ningbo First HospitalThe Affiliated Hospital of Zhejiang UniversityNingbo315000China College of Material Chemistry and Chemical EngineeringKey Laboratory of Organosilicon Chemistry and Material TechnologyMinistry of EducationHangzhou Normal UniversityHangzhou311121China Anhui Shenghaitang Traditional Chinese Medicine Decoction Pieces Co. LtdBozhou236800China Anhui Topway Testing Services Co. Ltd.Xuancheng Economic and Technological Development ZoneXuancheng242000China

Urothelial carcinoma(UC)is a common malignant tumor in the urinary system with high recurrence rate and low survival rate 5 years after *** present,imaging examination and other diagnostic methods have some shortcomings such as invasiveness and ***,it is urgent to develop a simple,rapid,noninvasive,highly sensitive and highly specific strategy to diagnose ***,a high-performance fluorescence sensor was constructed by the plasmonic gold nanorods(AuNRs)-enhanced near-infrared(NIR)fluorescence of silver sulfide quantum dots(Ag_(2)S QDs).The designed sensor can be used for the fast and accurate detection of small molecule single-transmembrane protein(FXYD3),which is overexpressed in 90%of ureteral cancers and 84%of high-grade bladder *** to its high specificity,the NIR fluorescence sensor achieves the detection of FXYD3 in the range of 0.25-150 ng·ml^(-1)with a detection limit of 0.2 ng·ml^(-1).Importantly,it also can be used for accurate diagnosis of FXYD3 in the urine of patients with relevant cancers,and the results are consistent with clinical cystoscopy and pathological *** proposed fluorescence sensor provides a simple,ultrasensitive,reliable method for UC screening,tumor-grade classification and postoperative monitoring and will have great potential for clinical applications.

关键词： Urothelial carcinoma FXYD3 Plasmonic enhanced fluorescence Ultrasensitive Accurate diagnosis

One-year status of hepatic venous pressure gradient measurement from 85 hospitals in China

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Portal Hypertension & Cirrhosis 2024年第2期3卷 116-123页

作者： Jitao Wang Ting Cui linpeng zhang Zhiping yan Lei li Jinjun Chen Junhui Sun hua Xiang He Zhu Hao Wu Jiancuo Gengzang Feng zhang Wei Wu Guohong cao Wuhua Guo Haibin Shi Hui Xue Guangjun huang Qiang Yu Meng Niu Huiling Xiang Derun Kong Wei Gou Xuefeng Luo Fuliang He Min Yuan Wei liu Yong Wang Hongjie Hu Xiaoli Zhu Tao Yu Qinxue Sun Wei Qu Ting Lu Deqiang Ma li zhang Jun Ma Jun yang Ketao Mu Xiaoliang Zhu Kai Xiong Huiguo Ding Shengjuan Yao Mingsheng huang Fang Wang Zexin Wang Yong huang Jianan li Hongfeng Yi Birun huang Zhongwei Zhao Duiping Feng yanming Lei Changlong Hou Wenbo Guo Shirong liu Gaojun Teng Kangshun Zhu Fan Wu Xiaogang Hu Wenfeng zhang Shaoqi yang zhouchao Hu Pengfei Pang Shaowu Zhuang Weidong Wang Qingliang Zhu Kunpeng zhang Chengyu liu Dongliang li Chao Ma Hong jiang Xingong Zhao Weixin Ren Zhiwei Wang Lei Yu Fuhuang lin Chaoguang yan Feng Wang Lei Yu Hui huan Wenyong Shen Shaofei He Chengda zhang Jinlun Bao jiangtao liu Jun Wu Jianbo Zhao Jian zhang Xiaolong Qi Hebei Provincial Key Laboratory of Cirrhosis&Portal Hypertension Xingtai People's HospitalXingtaiHebeiChina Center of Portal Hypertension Department of RadiologyZhongda HospitalSchool of MedicineSoutheast UniversityNanjingJiangsuChina Department of Interventional Therapy Beijing Shijitan Hospital Affiliated to Capital Medical UniversityBeijingChina Department of Intervention Taiyuan Third People's HospitalTaiyuanShanxiChina Department of Intervention Zhongshan Hospital Affiliated to Fudan UniversityShanghaiChina Department of Interventional Medicine The First Hospital of Lanzhou UniversityLanzhouGansuChina Department of Infectious Diseases Nanfang Hospital of Southern Medical UniversityGuangzhouGuangdongChina Hepatobiliary and Pancreatic Intervention Center The First Affiliated Hospital of Zhejiang University School of MedicineHangzhouZhejiangChina Interventional Vascular Surgery Hunan Provincial People's HospitalChangshaHunanChina Department of Intervention Sixth People's Hospital of ShenyangShenyangLiaoningChina Department of Gastroenterology West China Hospital of Sichuan UniversityChengduSichuanChina Department of Intervention The Fourth People's Hospital of Qinghai ProvinceXiningQinghaiChina Department of Gastroenterology Nanjing Drum Tower HospitalNanjingJiangsuChina Department of Gastroenterology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiangChina Department of Radiology Shulan(Hangzhou)HospitalHangzhouZhejiangChina Department of Intervention Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhouFujianChina Department of Interventional Radiology The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsuChina Department of Gastroenterology The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'anShaanxiChina Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinanShandongChina Department of Interventional Radiology Fifth Medical Center

Aims:Surveys and research on the applications of the hepatic venous pressure gradient(HVPG)are important for understanding the current status and future development of this technology in *** article aimed to investigate the status of hepatic venous pressure gradient measurement in China in ***:We investigated the overall status of HVPG technology in China-including hospital distribution,hospital level,annual number of cases,catheters used,average cost,indications,and current challenges by using online *** counting the number and percentages of cases of these results,we hope to clarify the current status of HVPG measurements in ***:According to the survey,85 hospitals in China used HVPG technology in 2022 distributed across 29 provinces.A total of 4989 HVPG measurements were performed in all of the surveyed hospitals in 2022,of which 2813 cases(56.4%)were measured *** average cost of HVPG measurement was 5646.8±2327.9 *** the clinical teams who performed the measurements(sometimes multiple per hospital),94.3%(82/87)used the balloon method,and the majority of the teams(72.4%,63/87)used embolectomy ***:This survey clarified the clinical application status of HVPG in China and confirmed that some medical institutions in China have established a foundation for this *** is still necessary to continue promoting and popularizing this technology in the future.

关键词： application status hepatic vein pressure gradient questionnaire survey

Long-term outcome of patients with atrial myxoma after surgical intervention: analysis of 403 cases

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Journal of Geriatric Cardiology 2019年第4期16卷 338-343页

作者： Chen-Xi jiang Jian-Gang WANG rui-Dong QI Wei WANG li-Jian GAO Jing-hua ZHAO Chun-Xiao zhang Meng-Chen zhou Xin TU Mei-Sheng SHANG yan YAO Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityBeijingChina Department of Cardiovascular Surgery Beijing Anzhen HospitalCapital Medical UniversityBeijingChina Center for Coronary Heart Disease Department of CardiologyCardiovascular Institute & Fuwai HospitalCAMS & PUMCNational Center for Heart DiseaseBeijingChina College of life Science and Technology Huazhong University of Science and TechnologyWuhanChina

Objective To assess long-term survival and late cardiovascular events in patients with atrial myxoma after surgical intervention. Methods Retrospective analysis of 403 patients undergoing resection of atrial myxoma from January 2002 to December 2016 was conducted with a median follow-up period of 4.5 (range: 0.5?15) years. Results The cross-clamp time and cardiopulmonary bypass times were 41.1 ± 21.4 and 65.2 ± 27.3 min,respectively. A diagnosis of myxoma was histopathologically confirmed in all cases. The early in-hospital mortality rate was 0.7%(n = 3). During the follow-up period,tumor recurrence occurred in six patients and cerebral infarction in nine. There were 48 (11.9%) patients with late onset atrial fibrillation (AF). By multivariate analysis,age (HR = 1.05,95% CI: 1.02–1.09,P lity. Conclusions Atrial myxoma can be resected with good long-term survival. Late onset AF is common after surgery in patients with atrial myxoma. Advanced age,left atrial diameter,and mitral valve surgery were independent predictors of outcomes.

关键词： Atrial fibrillation Atrial myxoma Embolism Survival

Knockdown of Microtubule Associated Serine/threonine Kinase like Expression Inhibits Gastric Cancer Cell Growth and Induces Apoptosis by Activation of ERK1/2 and Inactivation of NF-κB Signaling

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Current Medical Science 2021年第1期41卷 108-117页

作者： Cai-xia AN Shou-pin XIE Hai-long li Yong-hua HU Rong NIU lin-jie zhang yan jiang Qiang li Yong-ning zhou Division of Gastroenterology and Hepatology The First Hospital of Lanzhou UniversityLanzhou 730000China Department of Neurology The First People’s Hospital of Lanzhou CityLanzhou 730050China Department of Internal Mddicine The First School of Clinical MedicineGansu University of Chinese MedicineLanzhou 730000China Department of External Chest Gansu Provincial Cancer HospitalLanzhou 730030China Division of Pediatric Emergency Gansu Provincial Maternal and Child Health HospitalLanzhou 730050China Division of Neurosurgery The Second Hospital of Lanzhou UniversityLanzhou 730000China

Microtubule-associated serine/threonine kinase(MASTL)functions to regulate chromosome condensation and mitotic ***,aberrant MASTL expression is commonly implicated in various human *** study analyzed MASTL expression in gastric cancer *** normal tissue for elucidating the association with clinicopathological data from *** work was then extended to investigate the effects of MASTL knockdown on tumor cells in *** level of MASTL expression in gastric cancer tissue was assessed from the UALCAN,GEPIA,and Oncomine online *** carrying MASTL or negative control shRNA was infected into gastric cancer ***-qPCR,Western blotting,cell viability,cell counting,flow cytometric apoptosis and cell cycle,and colony formation assays were *** was upregulated in gastric cancer tissue compared to the adjacent normal tissue,and the MASTL expression was associated with advanced tumor stage,Helicobacter pylori infection and histological *** the other hand,knockdown of MASTL expression significantly reduced tumor cell viability and proliferation,and arrested cell cycle at G2/M stage but promoted tumor cells to undergo *** protein level,knockdown of MASTL expression enhanced levels of cleaved PARP1,cleaved caspase-3,Bax and p-ERK1/2 expression,but downregulated expression levels of BCL-2 and p-NF-κB-p65 protein in AGS and MGC-803 *** overexpression in gastric cancer tissue may be associated with gastric cancer development and progression,whereas knockdown of MASTL expression reduces tumor cell proliferation and induces *** study will evaluate MASTL as a potential target of gastric cancer therapeutic strategy.

关键词： gastric cancer microtubule-associated serine/threonine kinase gene expression shRNA

基于白光中子源的^(197)Au中子辐射俘获截面测量及共振参数分析

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物理学报 2024年第7期73卷 104-113页

作者：罗淏天张奇玮栾广源王晓宇邹翀任杰阮锡超贺国珠鲍杰孙琪黄翰雄王朝辉吴鸿毅顾旻皓余滔解立坤陈永浩安琪白怀勇鲍煜曹平陈昊磊陈琪萍陈裕凯陈朕崔增琪樊瑞睿封常青高可庆韩长材韩子杰何泳成洪杨黄蔚玲黄锡汝季筱璐吉旭阳蒋伟江浩雨姜智杰敬罕涛康玲康明涛李波李超李嘉雯李论李强李晓李样刘荣刘树彬刘星言穆奇丽宁常军齐斌斌任智洲宋英鹏宋朝晖孙虹孙康孙晓阳孙志嘉谭志新唐洪庆唐靖宇唐新懿田斌斌王丽娇王鹏程王琦王涛峰文杰温中伟吴青彪吴晓光吴煊羊奕伟易晗于莉于永积张国辉张林浩张显鹏张玉亮张志永赵豫斌周路平周祖英朱丹阳朱科军朱鹏朱兴华中国原子能科学研究院核数据重点实验室北京102413 中国科学院高能物理研究所北京100049 散裂中子源科学中心东莞523803 核探测与核电子学国家重点实验室北京100049 中国科学技术大学近代物理系核探测与核电子学国家重点实验室合肥230026 北京大学物理学院核物理与核技术国家重点实验室北京100871 中国工程物理研究院核物理与化学研究所绵阳621900 西北核技术研究院西安710024 中国科学院大学北京100049 中国科学技术大学工程与应用物理系合肥230026 北京航空航天大学物理学院北京100083 华能山东石岛湾核电有限公司荣成264312

中子辐射俘获反应在反应堆运行、核装置设计及核天体物理研究中起重要的作用.4πBaF_(2)探测装置有着高时间分辨能力、低中子灵敏度、高探测效率等优点,适合开展中子辐射俘获反应截面数据的测量.中国原子能科学研究院核数据重点实验室... 详细信息

中子辐射俘获反应在反应堆运行、核装置设计及核天体物理研究中起重要的作用.4πBaF_(2)探测装置有着高时间分辨能力、低中子灵敏度、高探测效率等优点,适合开展中子辐射俘获反应截面数据的测量.中国原子能科学研究院核数据重点实验室建立了伽马全吸收装置(Gamma total absorption facility,GTAF),该装置用28块六棱BaF_(2)晶体和12块五棱BaF_(2)晶体构成了外径25 cm,内径10 cm的球壳,覆盖了95.2%的立体角.利用GTAF在中国散裂中子源Back-n束线上,测量了197Au(n,γ)的反应截面数据.测量数据通过能量筛选、PSD方法、晶体多重性筛选进行了初步本底扣除,随后结合对^(nat)C及空样品的测量数据对本底进行了分析及扣除,获得了197Au俘获反应的产额,利用SAMMY程序拟合得到了^(197)Au在1—100 e V的共振能量、中子共振宽度和伽马共振宽度参数.实验测量结果与ENDF/B-VIII.0数据库符合良好,其共振参数存在一定差异,分析原因可能与GTAF能量分辨率、Back-n的中子能谱测量精度、以及实验本底扣除方法相关,这也是下一步工作的重点.

关键词：中子辐射俘获截面共振参数白光中子源伽马全吸收装置

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Comorbidity Burden of Dementia:A Hospital-Based Retrospective Study from 2003 to 2012 in Seven Cities in China

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Neuroscience Bulletin 2017年第6期33卷 703-710页

作者： Qing-hua Wang Xin Wang Xian-Le Bu yan lian yang Xiang Hong-Bo Luo Hai-Qiang Zou Jie Pu Zhong-He zhou Xiao-Ping Cui Qing-Song Wang Xiang-Qun Shi Wei Han Qiang Wu Hui-Sheng Chen Hang lin Chang-Yue Gao li-li zhang Zhi-Qiang Xu Meng zhang hua-Dong zhou yan-jiang Wang Department of Neurology and Center for Clinical Neuroscience Daping HospitalThird Military Medical UniversityChongqingChina Department of Preventive Medicine Daping HospitalThird Military Medical UniversityChongqingChina Department of Neurology Chengdu General Hospital of the PLAChengduChina Department of Neurology Lanzhou General Hospital of the PLALanzhouChina Department of Neurology Guangzhou General Hospital of the PLAGuangzhouChina Department of Neurology Wuhan General Hospital of the PLAWuhanChina Department of Neurology Shenyang General Hospital of the PLAShenyangChina Department of Neurology Fuzhou General HospitalFujian Medical UniversityFuzhouChina Department of Prevention Medicine Guangzhou General Hospital of the PLAGuangzhouChina

Dementia is increasing dramatically and imposes a huge burden on society. To date, there is a lack of data on the health status of patients with dementia in China. In an attempt to investigate the comorbidity burden of dementia patients in China at the national level, we enrolled 2,938 patients with Alzheimer＇s disease（AD）, vascular dementia（Va D）, or other types of dementia, who were admitted to tertiary hospitals in seven regions of China from January2003 to December 2012. The Charlson Comorbidity Index（CCI） was used to evaluate the comorbidity burden of the patients with dementia. Among these patients, 53.4% had AD, 26.3% had Va D, and 20.3% had other types of dementia. The CCI was 3.0 ± 1.9 for all patients,3.4 ± 1.8 for those with Va D, and 3.0 ± 2.1 for those with AD. The CCI increased with age in all patients, andthe length of hospital stay and daily expenses rose with age and CCI. Males had a higher CCI and a longer stay than females. Moreover, patients admitted in the last 5 years of the study had a higher CCI than those admitted in the first 5 years. We found that the comorbidity burden of patients with dementia is heavy. These findings provide a better understanding of the overall health status of dementia patients, and help to increase the awareness of clinicians and policy-makers to improve medical care for patients.

关键词： Alzheimer’s disease Vascular dementia Prevalence Comorbidity Charlson comorbidity index

Molecular Epidemiological Characteristics of Streptococcus pyogenes Strains Involved in an Outbreak of Scarlet Fever in China,2011

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Biomedical and Environmental Sciences 2013年第11期26卷 877-885页

作者： YOU Yuan Hai SONG yan yan yan Xiao Mei WANG Hai Bin zhang Meng Han TAO Xiao Xia li Lei Lei zhang Yu Xin jiang Xi Hong zhang bing hua zhou Hao XIAO Di JIN lian Mei FENG Zi Jian LUO Feng Ji zhang Jian Zhong Collabroative Innovation Center for Diagnosis Treatment of Infectious Disease State Key Laboratory for Infectious Disease Prevention and ControlNational Institute for Communicable Disease Control and Prevention Chaoyang District Center for Disease Control and Prevention Beijing Suzhou City Center for Disease Control and Prevention Office of Disease Control and Emergency Response Chinese Center for Disease Control and Prevention Harbin Municipal Center for Disease Control and Prevention

Objective To investigate molecular characterization of streptococcus pyogenes isolates involved in an outbreak of scarlet fever in China in 2011. Methods Seventy-four 5treptococcol pyogenes involved in an outbreak of scarlet fever were isolated from pediatric patients in the areas with high incidence in China from May to August of 2011. Emm genotyping, pulsed-field gel electrophoresis （PFGE）, superantigen （SAg） genes and antimicrobial susceptibility profiling were analyzed for these isolates. Results A total of 4 different emm types were identified. Emm12 was the most prevalent type which contained four predominating PFGE patterns corresponding to four different virulence and superantigen profiles. Emm12（79.7%） and emml （14.9%） accounted for approximately 94% of all the isolates. The speA gene was all negative in emm12 isolates and positive in emml isolates. All strains were resistant to erythromycin, and 89.4% of them were resistant to erythromycin, tracycline, and clindamycin simultaneously. Conclusion Several highly diversified clones with a high macrolide resistance rate comprise a predominant proportion of circulating strains, though no new emm type was found in this outbreak. The data provide a baseline for further surveillance of scarlet fever, which may contribute to the explanation of the outbreak and development of a GAS vaccine in China.

关键词： Scarlet fever Streptococcus pyogenes Molecular epidemiology

Genetic Architecture and Functional Implications of the CSF‑Contacting Nucleus

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Neuroscience Bulletin 2023年第11期39卷 1638-1654页

作者： Siyuan Song Yumin Yuan lingling Xu Jun jiang Ying li Yao yan Qing li Fang zhou Junli cao licai zhang jiangsu Province Key Laboratory of Anesthesiology Xuzhou Medical UniversityXuzhou 221004China jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology Xuzhou Medical UniversityXuzhou 221004China NMPA Key Laboratory for Research and Evaluation of Narcotic and Psychotropic Drugs Xuzhou 221008China School of Nursing Xuzhou Medical UniversityXuzhou 221004China

We previously identified a unique nucleus,the cerebrospinal fluid(CSF)-contacting *** study aims to understand its gene architecture and preliminarily suggest its *** results showed that there were about 19,666 genes in this nucleus,of which 913 were distinct from the dorsal raphe nucleus(non-CSF contacting).The top 40 highly-expressed genes are mainly related to energy metabolism,protein synthesis,transport,secretion,and *** main neurotransmitter is *** receptors of 5-HT and GABA are *** channels for Cl–,Na+,K+,and Ca2+are routinely *** signaling molecules associated with the CaMK,JAK,and MAPK pathways were identified *** particular,the channels of transient receptor potential associated with nociceptors and the solute carrier superfamily members associated with cell membrane transport were significantly *** relationship between the main genes of the nucleus and life activities is preliminarily verified.

关键词： Gene architecture Cerebrospinal fluidcontacting nucleus(CSF-contacting nucleus) Biological function