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Frontiers of Environmental Science & Engineering 2013年第3期7卷 435-441页

作者： ming hua Lili xiao Bingcai PAN Quanxing ZHANG State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Xianlin Campus Nanjing University Nanjing 210023 China

The efficient removal of phosphorous from water is an important but challenging task. In this study, we validated the applicability of a new commercially available nanocomposite adsorbent, i.e., a polymer-based hydrated ferric oxide nanocomposite （HFO-201）, for the further removal of phosphorous from the bioefftuent discharged from a municipal wastewater treatment plant, and the operating parameters such as the flow rate, temperature and composition of the regenerants were optimized. Labora- tory-scale results indicate that phosphorous in real bioeffluent can be effectively removed from 0.92 mg· L^-1 to 〈 0.5 mg· L^-1 （or even 〈 0.1 mg·L^-1 as desired） by the new adsorbent at a flow rate of 50 bed volume （BV） per hour and treatable volume of 3500-4000BV per run. Phosphorous removal is independent of the ambient temperature in the range of 15℃-40℃. Moreover, the exhausted HFO-201 can be regenerated by a 2% NaOH ＋ 5% NaC1 binary solution for repeated use without significant capacity loss. A scaled-up study further indicated that even though the initial total phosphorus （TP） was as high as 2 mg·L^-1, it could be reduced to 〈 0.5 mg·L^-1, with a working capacity of 4.4-4.8 g·L^-1 HFO- 201. In general, HFO-201 adsorption is a choice method for the efficient removal of phosphate from biotreated waste effluent.

关键词： bioeffluent phosphorus removal nanocom-posite adsorbent hydrated ferric oxide

MIMO-OFDM channel estimation method utilizing correlation in time domain for B3G-TDD uplink

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The Journal of China Universities of Posts and Telecommunications 2007年第2期14卷 23-27页

作者： ZHOU ming-yu LI Li-hua JIANG Jun ZHONG ming-hua TAO xiao-feng Wireless Technologies Innovation Institute Beijing University of Posts and Telecommunications Beijing 100876 China

This article proposes a simple pilot-aided channel estimation method based on correlation in time domain for multiple-input and multiple-output olthogonal frequency division multiplexing （MIMO-OFDM） systems. Pilot symbols in all transmit antennas are generated from different circular shifting of a certain sequence. Through once correlation, the receiver can obtain time-domain pulse responses for channel fading from all transmit antennas to a certain receive antenna, from which channel estimation in frequency domain can be obtained. Beyond 3G time-division duplex （B3G-TDD） uplink is introduced, and the channel estimation method is used in it. Theoretical analysis and simulation are both carried out. Mean square error （MSE） performance shows that the method can exhibit precise estimation. Complexity analysis proves it requires very low complexity. System simulation result shows that it guarantees the performance of B3G-TDD uplink very well.

关键词： multiple-input and multiple-output (MIMO) orthogonal frequency division multiplexing (OFDM) channel estimation time-domain pulse response

Mechanisms of Enhanced Rice Growth and Nitrogen Uptake by Nitrate

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Pedosphere 2007年第6期17卷 697-705页

作者： DUAN Ying-hua YIN xiao-ming ZHANG Ya-Li SHEN Qi-Rong College of Resources and Environmental Sciences Nanjing Agricultural University Nanjing 210095 （China）

Rice is being increasingly cultivated in intermittently irrigated regions and also in aerobic soil in which Nitrate （NO3^- ） plays important role in nutrition of plant. However, there is no information regarding the influence of nitrate on the overall growth and uptake of nitrogen （N） in rice plant. Solution culture experiments were carried out to study the effects of NO^3- on the plant growth, uptake of N, and uptake kinetics of NH4^＋ in four typical rice （Oryza sativa L.） cultivars （conventional indica, conventional japonica, hybrid indica, and hybrid japonica）, and on plasma membrane potential in roots of two conventional rice cultivars （indica and japonica） at the seedling stage. The results obtained indicated that a ratio of 50/50 NH4^＋-N/NO3^--N increased the average biomass of rice shoots and roots by 20% when compared with that of 100/0 NH4^＋-N/NO3^--N. In case of the 50/50 ratio, as compared with the 100/0 ratio, total N accumulated in shoots and roots of rice increased on an average by 42% and 57%, respectively. Conventional indica responds to NO3^- more than any other cultivars that were tested. The NO^3- supply increased the maximum uptake rate （Vmax） of NH4^＋ by rice but did not show any effect on the apparent Michaelis-Menten constant （Km） value, with the average value of Vmax for NH4^＋ among the four cultivars being increased by 31.5% in comparison with those in the absence of NO3^-. This suggested that NO3^- significantly increased the numbers of the ammonium transporters. However, the lack of effect on the Km value also suggested that the presence of NO3^- had no effect on the affinity of the transporters for NH4^＋. The plasma membrane potential in the roots of conventional indica and japonica were greatly increased by the addition of NO3^- , suggesting that NO3^- could improve the uptake of N by roots of the rice plant. In conclusion, the mechanisms by which NO3^- enhances the growth and N uptake of rice plant was found by the increased value of Vm

关键词： ammonium uptake nitrate nutrition nitrogen uptake plasma membrane potential rice

Analysis of Arc Physical Property of Pulsed Tungsten Inert Gas Welding Based on Fowler-Milne Method

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Journal of Shanghai Jiaotong university(Science) 2013年第3期18卷 343-347页

作者： WANG Lin hua Xue-ming xiao xiao LI Fang WU Yi-xiong Welding Engineering Institute Shanghai Jiaotong University Shanghai Key Laboratory of Materials Laser Manufacturing and Modification Shanghai 200240 China

Pulsed tungsten inert gas （TIG） welding is widely used in industry due to its superior properties, so the measurement of arc telnperature is important to analyse welding process. Arc image of spectral line in 794.8 nm is captured by high speed camera; both the Abel inversion and the Fowler-Milne method are used to calculate the temperature distribution of the pulsed TIG welding. Characteristic of transient variation in arc intensity and temperature is analyzed. When the change of current happens, intensity and temperature of arc jump as well, it costs several millisoconds. The flirther the axial position from the tungsten is, the greater the intensity jumps, and the smaller the temperature changes.

关键词： pulsed tungsten inert gas (TIG) welding Fowler-Milne method arc temperature transient variation

Simultaneous determination of indole metabolites of tryptophan in rat feces by chemical labeling assisted liquid chromatography-tandem mass spectrometry

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Chinese Chemical Letters 2022年第11期33卷 4746-4749页

作者： Qin-Feng Zhang hua-ming xiao Jin-Tao Zhan Bi-Feng Yuan Yu-Qi Feng Department of Chemistry Wuhan UniversityWuhan 430072China School of Public Health Wuhan UniversityWuhan 430071China

As the connecting part of diet and host physiology,intestinal microbes can convert the ingested diet into a huge number of physiologically active small *** metabolites of tryptophan are precursors or signal molecules for many biologically active substances,which are involved in serotonin and microbial catabolism *** understand the influence of tryptophan metabolism in the intestinal environment on the neurological and immune systems at the molecular level,it is important to establish a high-coverage analytical method to comprehensively analyze the metabolites involved in tryptophan ***,due to a small molecular weight and poor response during mass spectrometry analysis,as well as weak retention on the reversed-phase chromatography,determination of indole metabolites of tryptophan is ***,we proposed a method for the simultaneous determination of 20 indole metabolites of tryptophan in a single run on reversed-phase chromatography by chemical labeling coupled to liquid chromatography-tandem mass spectrometry analysis.4-(Dimethylamino)benzaldehyde(DMAB)was used for the labeling of indole metabolites of tryptophan,which could significantly improve the detection sensitivities and retention of these metabolites on reversed-phase *** the developed method,we realized the sensitive detection and comprehensive analysis of 15 endogenous indole metabolites of tryptophan in rat feces samples with functional dyspepsia intervention by *** developed method offers a useful tool for studying tryptophan metabolism-related diseases.

关键词： Chemical labeling Liquid chromatography-mass spectrometry Indole metabolites Tryptophan Rat feces

Fluoro-functionalized plant biomass adsorbent:Preparation and application in extraction of trace perfluorinated compounds from environmental water samples

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Journal of Environmental Sciences 2024年第3期137卷 703-715页

作者： Xi Tan Yan Shi Chun-Feng Ma Quan Chi Yu-Hang Yang Wen-Xiang Zhang hua-ming xiao Xian Wang Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission School of Chemistry and Materials ScienceSouth-Central Minzu UniversityWuhan 430074China School of Pharmaceutical Sciences South-Central Minzu UniversityWuhan 430074China Key Laboratory of Oilseeds Processing of Ministry of Agriculture Hubei Key Laboratory of Lipid Chemistry and NutritionOil Crops Research Institute of the Chinese Academy of Agricultural SciencesWuhan 430062China

Perfuorinated compounds(PFCs)are toxic and widely present in the environment,and therefore effective adsorbents are required to remove PFCs from environmental *** the present study,a new type of fuorinated biomass materials was synthesized via an ingenious fuorosilanization *** adsorbents were applied for the adsorption of 13typical PFCs,including perfuorocarboxylic acids(PFCAs)and perfuorosulfonic acids(PFSAs).By comparing their adsorption performance,Fluorinated cedar slag(FCS)was discovered to have the best absorption efficiency and enabled highly efficient enrichment of *** adsorption recovery of FCS with the investigated PFCs is greater than 90%under the optimal adsorption *** to the high affinity of F-F sorbent-sorbate interaction,FCS had good adsorption capacities of PFCs from aqueous solution,with the maximum adsorption capacity of 15.80 mg/g for PFOS and 10.71 mg/g for PFOA,***,the adsorption time could be achieved in a short time(8 min).Using the FCS absorbent,an innovative FCS-solid phase extraction assisted with high performance liquid chromatography-electrospray-tandem mass spectrometry(FCS-SPE-HPLC-ESI-MS/MS)method was first developed to sensitively detect PFCs in the environmental water *** intra-day and inter-day recovery rates of the 13 compounds ranged from 90.7%-104.3%,with the RSD of 2.1%-4.7%(intra-day)and 2.5%-8.5%(inter-day),*** research demonstrates the potential of the newly fuoro-functionalized plant biomass to adsorb PFCs from environmental water,with the advantages of high adsorption efficiencies,high antiinterference,easy operation and low economic cost.

关键词： Fluoro-functionalized biomass Perfuorinated compounds(PFCs) Adsorption efficiency Mass spectrometry(MS) Fluorinated cedar slag(FCS)

Immunoregulatory Effects of Ethyl-acetate Fraction of Extracts from Tetrastigma Hemsleyanum Diels et. Gilg on Immune Functions of ICR Mice

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Biomedical and Environmental Sciences 2008年第4期21卷 325-331页

作者： CAI-JU XU GANG-QIANG DING JIAN-YUN FU JIA MENG RONG-hua ZHANG xiao-ming LOU Department of Toxicology Zhejiang Provincial Center for Disease Control and Prevention Hangzhou 310009 Zhejiang China

Objective To evaluate the effects of ethyl-acetate fraction （EAF） of extracts from Tetrastigma hemsleyanum Diels et. Gilg （TDG） on immune functions of ICR mice. Methods ICR mice were exposed to different doses of EAF for 15 or 30 days and then their immune functions were analyzed, including ConA-induced splenic lymphocyte transformation, SRBC- induced delayed type hypersensitivity response, serum hemolysin analysis, antibody-producing cells, peritoneal macrophage phagocytized chicken red blood cells, natural killer cell activity, and serum level of cytoldnes. Results EAF of extracts from TDG at different doses had various effects on immune functions of ICR mice. As compared with the controls, it increased the mouse spleen lymphocyte transformation induced by ConA, the left-hind voix pedis thickness and the number of plague forming cells （PFCs） at the dose of 1.82 mg/mL, 5.48 mg/mL, and 9.12 mg/mL, respectively; increased the ink clearance ability at the dose of 0.91 mg/mL, 1.82 mg/mL, 5.48 mg/mL, and 9.12 mg/mL, respectively; increased the phagocytosis index of mononuclear-macrophages and production of serum interferon-gamma （IFN-？） at the dose of 5.48 mg/mL; and could promote the production of serum tumor necrosis factor-alpha （TNF-α） at the dose of 9.12 mg/mL. Conclusion EAF of extracts from TDG can regulate mouse immune functions in vivo.

关键词： EAF of extracts from TDG Immuno-regulation Cellular immunity Humoral immunity Macrophage phagocytosis NK cytoactivity Serum cytokines

Comparison of Effects of Recombinant FSH versus Highly Purified Urinary FSH for COH in the Outcome of IVF-ET

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Journal of Reproduction and Contraception 2011年第2期22卷 89-94页

作者： Xian-dong PENG hua CHEN ming YU xiao-xi SUN Shanghai JIAI Genetics ＆ IVF Institute Shanghai 200011 China

Objective To investigate the difference in the outcome of IVF-ET between the patients using recombinant FSH versus highly purified urinary FSH for controlled ovarian stimulation （COH）. Methods A comparative retrospective analysis was performed between the patients undergoing IVF using recombinant FSH （rFSH） 562 cycles （rFSH group） versus using highly purified urinary FSH（uFSH） 555 cycles （uFSH group） for COH from August 2009 to July 2010. Main outcome measures included： duration of stimulation, total amount of FSH, E2 level on hCG injection day, number of oocytes collected, average number of frozen embryos, and the rate of cancellation, implantation, clinical pregnancy and miscarriage rate. Results uFSH group required a significantly higher total amount of FSH and a longer duration of stimulation than thouse rFSH group （19.3 ± 7.6 vs 18.3 ± 7.0 ampoules, P=0.02, and 9.1 ± 1.6 d vs 8.9 ± 1.5 d, P=0.04, respectively）. There was no statistically significant difference in the implantation rate, clinical pregnancy rate and miscarriage rate between uFSH and rFSH groups （22.57% vs 20.16%, 38.15% vs 35.18%, and 10.6% vs 13.45%, respectively, P〉0.05）. The other measures including E2 level on hCG injection day, cancellation, number of oocytes collected, fertilization, average number of frozen embryos showed no difference between the two groups. Conclusion There is no significant difference in the outcome of IVF-ET between two groups in effects of recombinant FSH versus highly purified urinary FSH for COH.

关键词： highly purified urinary FSH (HP-uFSH) recombinant FSH IVF outcome

Upregulation of S100A4 after spinal cord transectionin adult rats

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Acta Pharmacologica Sinica 2004年第8期 34-39页

作者： Kai-hua ZHANG, Shu HAN, Pei-hua LU, xiao-ming XUDepartment of Neurobiology, Shanghai Second Medical University, Shanghai 200025, China Kentucky Spinal Cord Injury Research Center, Department of Neurological Surgery and Anatomical Sciencesand Neurobiology, University of Louisville School of Medicine, Louisville, KY 40292, USA

AIM: To investigate whether spinal cord transection induces changes of gene expression of S100A4 protein. METHODS: In a spinal cord transection model, S100A4 expression and cellular localization were examined using cDNA microarray, Northern blot, immunohistochemistry, and immunofluorescence double-labeling methods. RESULTS: There was very limited S100A4 mRNA expression in the control spinal cord. However, S100A4 mRNA expression was increased significantly in both the rostral and caudal spinal cord segments adjacent to the injury site. Specifically, S100A4 gene expression was substantially increased at d 2, peaked at d 7 and d 14, and remained high up to 28 d post-injury. During its peak expression, S100A4 protein was localized in astrocytes of the spinal cord within 5 mm from the site of spinal transection. CONCLUSION: Spinal cord transection induces prolonged S100A4 expression at both mRNA and protein levels in areas close to the injury site. Increased expression of S100A4 in astrocytes after spinal cor

关键词： spinal cord injury S100A4 gene expression

Abnormal expression of hepatomas and circulating telomerase and its clinical values

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Hepatobiliary & Pancreatic Diseases International 2005年第4期4卷 544-549页

作者： Wei Wu, Deng-Fu Yao, Li-Wei Qiu, Xin-hua Wu, ming Yao, xiao-Qin Su and Li Zou Nantong, China Research Center of Clinical Molecular Biology, Affiliated Hospital, Nantong University, Nantong 226001, China Research Center of Clinical Molecular Biology Affiliated Hospital Nantong University Nantong 226001 China.

BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant tumours worldwide. Te-lomerase is reactivated in various types of malignant tumors and may contribute to the development of HCC. To evaluate the role of telomerase in formation and development of HCC, we analyzed its expression status in different parts of HCC tissues and peripheral blood mono-nuclear cells (PBMCs) , and explored its clinical implications for diagnosis of HCC. METHODS: Total RNAs and telomerase were extracted from HCC and their non-cancerous tissues, and both relationships were analyzed between them. The expression of telomerase reverse transcriptase (hTERT) mRNA and telomerase activities in liver tissues and PBMCs were detected by RT-PCR and telomeric repeat amplification protocol (TRAP)-ELISA, respectively. The diagnostic values of telomerase in PBMCs were investigated in the diagnosis and differentiation of HCC. RESULTS: The specific activities of telomerase were 18.25 ±15.02 A/μg RNA in HCC tissues, and significantly higher than those in their non-cancerous tissues (8.16 ± 6.22 A/μg RNA, P <0. 05). But total RNA levels in HCC were 12.40 ±7.34 μg/mg wet liver and lower than 53.77 ± 52.02 μg/mg wet liver in their non-cancerous parts (P < 0.01). The different telomerase levels could be detected in PBMCs from patients with chronic liver diseases and control group. The enzyme activities were significantly higher in HCC than in any other groups (P <0.01). However, circulating telomerase levels were more obviously decreased in HCC patients after transcatheter arterial embolization treatment than before the treatment (P <0.01). The analysis of combined serum alpha-fetoprotein level and PBMCs telomerase was more sensitive and specific for the diagnosis of HCC. CONCLUSION: The abnormal expression of telomerase in HCC tissues and circulating PBMCs could be a useful marker to the diagnosis and prognosis of HCC.

关键词： telomerase peripheral blood TRAP-ELISA hepatocellular carcinoma