Upregulation of S100A4 after spinal cord transectionin adult rats

作     者：Kai-hua ZHANG, Shu HAN, Pei-hua LU, Xiao-ming XUDepartment of Neurobiology, Shanghai Second Medical University, Shanghai 200025, China Kentucky Spinal Cord Injury Research Center, Department of Neurological Surgery and Anatomical Sciencesand Neurobiology, University of Louisville School of Medicine, Louisville, KY 40292, USA 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第8期

页      面：34-39页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：ThisworkwassupportedbyShanghaiScienceandTechnologyDevelopmentFund(OOJC14021) 

主　　题：spinal cord injury S100A4 gene expression 

摘      要：AIM: To investigate whether spinal cord transection induces changes of gene expression of S100A4 protein. METHODS: In a spinal cord transection model, S100A4 expression and cellular localization were examined using cDNA microarray, Northern blot, immunohistochemistry, and immunofluorescence double-labeling methods. RESULTS: There was very limited S100A4 mRNA expression in the control spinal cord. However, S100A4 mRNA expression was increased significantly in both the rostral and caudal spinal cord segments adjacent to the injury site. Specifically, S100A4 gene expression was substantially increased at d 2, peaked at d 7 and d 14, and remained high up to 28 d post-injury. During its peak expression, S100A4 protein was localized in astrocytes of the spinal cord within 5 mm from the site of spinal transection. CONCLUSION: Spinal cord transection induces prolonged S100A4 expression at both mRNA and protein levels in areas close to the injury site. Increased expression of S100A4 in astrocytes after spinal cor