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Establishment and application of a detection kit for human carcinoembryonic antigen mRNA by real-time RT-PCR

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Establishment and application of a detection kit for human c...

2020长三角老年医学高峰论坛、浙江省老年医学学术大会、华东老年医学中心联盟会议、浙江省老年医学联盟会议、浙江省老年病专科联盟会议

作者： Yang Wenwen Cheng Min hu shilian

Background Progress in gastric cancer is hampered by the lack of diagnostic markers useful for clinical *** study aims to establish a quantitative method for human carcino-embryonic antigen(CEA) mRNA by real-time *** application was further evaluated by detecting circulating tumor cells in the peripheral blood of gastric cancer *** and Results The concentration of each component in the detection kit was optimized;the stability,sensitivity,and specificity were *** expression level of CEA mRNA was detected in the samples of peripheral *** primers and probes were obtained,and setting standards were successfully *** establishing the positive and negative control materials,the specificity,sensitivity,accuracy and quality control were determined for the detection *** specificity and accuracy were both more than 95%,and the sensitivity was of 100 copies/ml(whole peripheral blood).The detection results of CEA mRNA in peripheral blood samples showed that all the samples were positive for 15 gastric cancer patients clinically diagnosed with metastasis;and 12 samples were positive and other 13 samples were negative among 25 gastric cancer patients without clinically diagnosed with metastasis or not;all the samples were negative for 10 gastric ulcer patients;and all the samples were negative for 10 healthy *** The quantitative RT-PCR assay for CEA mRNA could be used for the detection of circulating tumor cells in peripheral blood of gastric cancer patients,which would be valuable for the diagnosis and clinicaldecision making for gastric cancer patients.

关键词： Gastric cancer Genetic marker Carcino-embryonic antigen mRNA RT-PCR

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CHFR基因与胃癌关系研究新进展

中国肿瘤临床 2009年第17期36卷 1016-1019页

作者：程昭栋胡世莲安徽医科大学附属省立医院合肥市230001

CHFR(Checkpoint with FHA and ring finger),是Scolnick和Halazonetis于2000年新发现的一种能够检测分裂期正常细胞的分裂情况及控制分裂进行的基因,是一个新的有丝分裂前期检查点基因,同时也是一种新发现的抑癌基因。CHFR编码一种具有... 详细信息

CHFR(Checkpoint with FHA and ring finger),是Scolnick和Halazonetis于2000年新发现的一种能够检测分裂期正常细胞的分裂情况及控制分裂进行的基因,是一个新的有丝分裂前期检查点基因,同时也是一种新发现的抑癌基因。CHFR编码一种具有FHA和RING区的蛋白,当微管抑制剂诱发有丝分裂应激时,这种蛋白具有延迟染色体的浓集、阻止细胞进入有丝分裂中前期的作用,在正常人组织中普遍表达,而在多种肿瘤组织中发现其表达缺失,而CpG岛的甲基化是导致CHFR表达缺失或沉默的重要机制。胃癌是世界上发病率最高的肿瘤之一,在肿瘤引起的死亡原因中占第二位。胃癌的发生过程涉及到许多基因和复杂的步骤,相关基因包括癌基因,抑癌基因,DNA修复基因,抑癌基因的功能由于基因突变、启动子的甲基化而丢失。甲基化发生于基因的启动子区时会导致基因的沉默,由此而引发肿瘤的发生。启动子区异常甲基化已经作为细胞恶变的一个独立的途径而被许多文献所报道,有研究显示胃癌的发生发展过程中也涉及许多抑癌基因的甲基化现象,其中包括CHFR基因。本文对CHFR基因的结构、编码蛋白的功能及其与胃癌关系的研究进展作一综述。

关键词：有丝分裂前期 CHFR基因胃癌甲基化抑癌基因

The ErbB2-overexpression tumor metastasis suppression by humanized antibody chA21 with new recognition epitope

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The ErbB2-overexpression tumor metastasis suppression by hum...

细胞—生命的基础——中国细胞生物学学会2013年全国学术大会·武汉

作者： Guodong Shen Siyi hu Zhao Zha Geng Bian hu shilian Jing Liu School of Life Science University of Science and Technology of China Gerontology Institute Anhui Provincial Hospital

It is widely known that ErbB2(HER2/p185) overexpression in human malignant cancers correlates with poor prognosis and the resistance against radiotherapy,chemotherapy and endocrine *** antibody drug trastuzumab(herceptin) is used in the treatment of clinical tumors with considerable success,but the insensitivity or resistant phenomenon in tumor therapy is increasingly *** a tumor-inhibitory anti-ErbB2 monoclonal antibody A21 and its

关键词： antibody drug ErbB2/Her2 trastuzumab chA21 breast cancer

Feedback regulatory loop of LAMTOR5-AS1/NRF2 in oxidative stress induced by chemotherapy

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Feedback regulatory loop of LAMTOR5-AS1/NRF2 in oxidative st...

2020长三角老年医学高峰论坛、浙江省老年医学学术大会、华东老年医学中心联盟会议、浙江省老年医学联盟会议、浙江省老年病专科联盟会议

作者： Dabing huang GuoDong Shen Ming Cheng Geng Bian shilian hu

As a kind of non coding RNA,long noncoding RNA(lncRNA) plays an important role in regulating cell biological *** high-througout sequencing analysis,we found that LAMTOR5-AS1 was highly expressed in osteosarcoma chemosensitive cell G-292,compared with chemoresistant cell *** also find that LAMTOR5-AS1 can significantly inhibit the proliferation and multidrug resistance of osteosarcoma *** database analysis showed that high expression of LAMTOR5-AS1 had better prognosis for patients aged10-20 years with ***,LAMTOR5-AS1 could promote the interaction between nuclear factor erythroid 2-related factor 2(NFE2 L2,NRF2) and Kelch-like ECH-associated protein 1(KEAP1) and regulate the activity of NRF2,KEAP1-NRF2 system plays an important role in oxidative ***5-AS1 regulates the expression and activity of NRF2 protein,which significantly affects the drug resistance of osteosarcoma *** the action of chemotherapeutic drugs,NRF2 has higher transcriptional activity in drugresistant cell lines and promotes the expression of its downstream gene Heme oxygenase 1(HO-1).What's more interesting is that LAMTOR5-AS1 acts as a caretaker rather than an executioner through reporter gene experiment and MG132 effect ***5-AS1 inhibited the ubiquitination degradation and the transcriptional activity of NRF2,which resulted in the increase of NRF2 protein expression and the loss of NRF2 transcriptional *** with the regulation of NRF2 from transcription to translation,LAMTOR5-AS1 plays a more rapid and important biological role in the regulation of NRF2 protein expression and function,alleviates oxidative stress caused by chemotherapy.

关键词： Feedback regulatory loop of LAMTOR5-AS1/NRF2 in oxidative stress induced by chemotherapy NRF

LncRNA FAM133B-2 represses radio-resistance of nasopharyngeal cancer cells by targeting miR-34a-5p/CDK6 axis

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LncRNA FAM133B-2 represses radio-resistance of nasopharyngea...

2020长三角老年医学高峰论坛、浙江省老年医学学术大会、华东老年医学中心联盟会议、浙江省老年医学联盟会议、浙江省老年病专科联盟会议

作者： Dabing huang XiaoDong Jiang Shi Yin Xiang Fang shilian hu

Long non-coding RNAs(lncRNAs) were found to play important roles in various cancers,including nasopharyngeal carcinoma(NPC).In our study,we focused on the biological function of the lncRNA FAM133 B-2 in NPC *** RNA-seq and qRT-PCR analysis showed that FAM133 B-2 is highly expressed in the radio-resistant NPC *** following biochemical assays showed that FAM133 B-2 represses the NPC radioresistance and also affects the apoptosis and proliferation of NPC *** investigations suggested that miR-34 a-5 p targets FAM133 B-2 and also regulates the cyclin-dependent kinase 6(CDK6).All these results suggested that the lncRNA FAM133 B-2 might function as a competitive endogenous RNA(ceRNA) for miR-34 a-5 p in NPC *** conclusion,our study demonstrated that FAM133 B-2 is up-regulated in radio-resistant NPC ***,we found that FAM133 B-2 is targeted by miR-34 a-5 p,which also negatively regulates the CDK6 *** these results provide new insights into NPC radio-resistance,and thus FAM133 B-2 might be a potential prognostic and diagnostic marker for NPC treatment.

关键词： LncRNA FAM133B-2 represses radio-resistance of nasopharyngeal cancer cells by targeting miR-34a-5p/CDK6 axis NPC FAM CDK miR

Analysis of the polymorphism of CYP2C19 gene inperipheral blood of the patients with coronary heart disease or stroke

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Analysis of the polymorphism of CYP2C19 gene inperipheral bl...

2020长三角老年医学高峰论坛、浙江省老年医学学术大会、华东老年医学中心联盟会议、浙江省老年医学联盟会议、浙江省老年病专科联盟会议

作者： Yang Wenwen Chen Wen Zha Yanfang Xu Tingjuan Shen Guodong Cheng Min hu shilian

Background and Objective:Clopidogrel,a cornerstone of antiplatelet therapy,in combination with aspirin is the standard of care recommended by many guidelines for the prevention and treatment of acute coronary syndrome(ACS),recurrent ischemic events in patients after percutaneous coronary intervention(PCI),and acute myocardial ***,the antiplatelet effects of clopidogrel vary greatly among different populations and *** inhibition of platelet aggregation is clinically reduced,and repeated cardiovascular events after standard antiplatelet therapy are known as clopidogrel *** causing clopidogrel resistance include polymorphism of metabolic genes,excessively low dose of clopidogrel,drug interactions,poor compliance,platelet factors,*** many factors,polymorphism of CYP2 C19 functionmissing gene is the main cause of clopidogrel resistance in ***,the CYP2 C19 genotype of patients taking clopidogrel was tested clinically,and drugs were selected reasonably according to the typing results,so as to reduce the occurrence of clinical adverse cardiovascular *** study analyzed the distribution characteristics of CYP2 C19 gene polymorphisms in patients with coronary heart disease or stroke,and discussed whether there were statistical differences in genotypes and metabolic types among different genders,ages and diseases,so as to provide evidence for the individualized application of platelet aggregation inhibitors in clinical ***:Nucleic acid extraction and purification kit,human CYP2 C19 genotyping assay kit and realtime fluorescence quantitative RT-PCR were used to analyze the genotypes and metabolites of 4270 patients with coronary heart disease and 141 patients with *** extracted,enriched and purified the peripheral blood samples,configured the response system and set the PCR routine,and used SPSS 22 to analyze the ***:Calculate the distribution of each genotype and metabolic

关键词： CYP2C19 genotype metabolic type coronary heart disease stroke

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健康检查在中老年慢性疾病早期诊治中的作用

临床中老年保健 2001年第4期4卷 221-222页

作者：胡世莲安徽省立医院合肥230001

目的　探讨健康检查在中老年常见慢性疾病防治中的价值。方法　总结近 4年 3次中老年健康检查资料 ,分析该人群常见慢性疾病患病情况及其并发症。结果　高血压 (HP)、糖尿病 (DM )、高脂血症、脑梗死等疾病患病率逐年增高 ,并随着年龄... 详细信息

目的　探讨健康检查在中老年常见慢性疾病防治中的价值。方法　总结近 4年 3次中老年健康检查资料 ,分析该人群常见慢性疾病患病情况及其并发症。结果　高血压 (HP)、糖尿病 (DM )、高脂血症、脑梗死等疾病患病率逐年增高 ,并随着年龄增长显著增高 ;高血压合并糖尿病患者的脑梗死、心肌梗死患病率明显增高。

关键词：中老年人慢性疾病健康检查并发症患病率心脑血管疾病

非小细胞肺癌化疗联合部分肠外营养对细胞免疫的影响

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老年医学与保健 2005年第4期11卷 226-229页

作者：胡世莲安徽省立医院老年医学科安徽合肥230001

目的通过对非小细胞肺癌(NSCLC)患者化疗期间应用部分肠外营养(PPN)支持治疗的前瞻性临床试验,阐明不同供氮量的营养支持方案对化疗期间机体细胞免疫功能的影响。方法将NSCLC患者随机分为三组:对照组、低剂量PPN组和高剂量PPN组。分别... 详细信息

目的通过对非小细胞肺癌(NSCLC)患者化疗期间应用部分肠外营养(PPN)支持治疗的前瞻性临床试验,阐明不同供氮量的营养支持方案对化疗期间机体细胞免疫功能的影响。方法将NSCLC患者随机分为三组:对照组、低剂量PPN组和高剂量PPN组。分别于化疗前后检测CD3+、CD4+、CD8+、NK细胞的百分率和CD4+/CD8+值。结果各组化疗后NK细胞、CD3+、CD4+的百分率与化疗前相比显著降低(P0.05)。结论化疗可降低NSCLC患者的细胞免疫功能,而给予含9-复合氨基酸的肠外营养支持可能阻止化疗期间细胞免疫功能的降低。

关键词：癌,非小细胞肺胃肠外营养抗肿瘤联合化疗方案 T淋巴细胞杀伤细胞,天然

A Positive Feedback Modulation of Wnt7B Expression by TCF7L2 Promotes the Metastasis of Gastric Cancer Cell

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A Positive Feedback Modulation of Wnt7B Expression by TCF7L2...

2020长三角老年医学高峰论坛、浙江省老年医学学术大会、华东老年医学中心联盟会议、浙江省老年医学联盟会议、浙江省老年病专科联盟会议

作者： Guodong Shen Qian Gao Liuyang Yang Geng Bian Min Cheng Tingjuan Xu Xinchun Wu shilian hu

Background:Wnt/β-catenin signalling plays an important role in cell growth and metastasis of gastric cancer（GC）,in which TCF7 L2 is well known as a key transcriptional *** we identified increased Wnt/β-catenin signalling activity in a highly metastatic gastric cancer cell line compared with its parental *** this study,we address the regulation mechanism of TCF7 L2 and the possible clinical ***:TCF7 L2 expression in tumors from 124 GC and 30 chronic gastritis patients was evaluated by ***-Meier and Cox regression analyses were applied to assess the association between overall survival and TCF7 L2 expression.6 gastric cancer cell lines SGC7901,HSC44-PE,44 As3,NC1-N87,MGC803 and MKN45 and an immortalized and non-tumorigenic gastric epithelial cell line GES-lwere included to analyze gene function and signal activity changes using western blotting,qualified RT-PCR and antibody array *** profiler PCR assay was used comparing mRNA expression changes of 84 Wnt signaling-related *** assay was used to determine the Wnt7 B releasing *** statistical tests were ***:TCF7 L2 expression was significantly higher in gastric cancer tissues than adjacent normal tissues（P<0.05）,which was significantly associated with depth of invasion,lymph node metastasis,TNM stage,and tumor size,but not grade of differentiation,gender,age,and tumor *** median progression-free survival（PFS） was 47 months and 65 months for patients with positive and negative TCF7 L2 expression,*** analysis revealed that positive TCF7 L2 expression was associated with worse prognosis（P=0.011,log-rank test）.Immunoblotting data showed that though TCF7 L2 was detected from almost all tested gastric cell lines,its expression was obviously higher in cancer cells than in normal *** knockdown or overexpression results demonstrated that high TCF7 L2 expression was correlated with the proliferation,migrati

关键词： Gastric cancer Wnt TCF7L2 Wnt7B Tumor metastasis