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Dynamics and genetic regulation of macronutrient concentrations during grain development in maize

Journal of Integrative Agriculture 2024年第3期23卷 781-794页

作者： Pengcheng Li Shuangyi Yin Yunyun wang Tianze Zhu Xinjie Zhu Minggang Ji Wenye Rui houmiao wang Chenwu Xu Zefeng Yang Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Agricultural CollegeYangzhou UniversityYangzhou 225009China Jiangsu Co-lnnovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009China

Nitrogen(N), phosphorus(P), and potassium(K) are essential macronutrients that are crucial not only for maize growth and development, but also for crop yield and quality. The genetic basis of macronutrient dynamics and accumulation during grain filling in maize remains largely unknown. In this study, we evaluated grain N, P, and K concentrations in 206 recombinant inbred lines generated from a cross of DH1M and T877 at six time points after pollination. We then calculated conditional phenotypic values at different time intervals to explore the dynamic characteristics of the N, P, and K concentrations. Abundant phenotypic variations were observed in the concentrations and net changes of these nutrients. Unconditional quantitative trait locus(QTL) mapping revealed 41 non-redundant QTLs, including 17, 16, and 14 for the N, P, and K concentrations, respectively. Conditional QTL mapping uncovered 39 non-redundant QTLs related to net changes in the N, P, and K concentrations. By combining QTL, gene expression, co-expression analysis, and comparative genomic data, we identified 44, 36, and 44 candidate genes for the N, P, and K concentrations, respectively, including GRMZM2G371058 encoding a Doftype zinc finger DNA-binding family protein, which was associated with the N concentration, and GRMZM2G113967encoding a CBL-interacting protein kinase, which was related to the K concentration. The results deepen our understanding of the genetic factors controlling N, P, and K accumulation during maize grain development and provide valuable genes for the genetic improvement of nutrient concentrations in maize.

关键词： maize nutrient concentration unconditional QTL mapping conditional QTL mapping dynamic trait

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Genome-wide association study identifies novel candidate loci or genes affecting stalk strength in maize

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The Crop Journal 2023年第1期11卷 220-227页

作者： Shuhui Xu Xiao Tang Xiaomin Zhang houmiao wang Weidong Ji Chenwu Xu Zefeng Yang Pengcheng Li Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding Yangzhou UniversityYangzhou 225009JiangsuChina Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009JiangsuChina National Maize Improvement Center of China China Agricultural UniversityBeijing 100193China State Key Laboratory of Crop Stress Adaptation and Improvement Key Laboratory of Plant Stress BiologySchool of Life SciencesHenan UniversityKaifeng 475001HenanChina

Stalk strength increases resistance to stalk lodging,which causes maize(Zea mays L.)production losses worldwide.The genetic mechanisms regulating stalk strength remain unclear.In this study,three stalk strength-related traits(rind penetrometer resistance,stalk crushing strength,and stalk bending strength)and four plant architecture traits(plant height,ear height,stem diameter,stem length)were measured in three field trials.Substantial phenotypic variation was detected for these traits.A genome-wide association study(GWAS)was conducted using general and mixed linear models and 372,331 single-nucleotide polymorphisms(SNPs).A total of 94 quantitative trait loci including 241 SNPs were detected.By combining the GWAS data with public gene expression data,56 candidate genes within 50 kb of the significant SNPs were identified,including genes encoding flavonol synthase(GRMZM2G069298,ZmFLS2),nitrate reductase(GRMZM5G878558,ZmNR2),glucose-1-phosphate adenylyltransferase(GRMZM2G027955),and laccase(GRMZM2G447271).Resequencing GRMZM2G069298 and GRMZM5G878558 in all tested lines revealed respectively 47 and 2 variants associated with RPR.Comparison of the RPR of the zmnr2EMS mutant and the wild-type plant under high-and low-nitrogen conditions verified the GRMZM5G878558 function.These findings may be useful for clarifying the genetic basis of stalk strength.The identified candidate genes and variants may be useful for the genetic improvement of maize lodging resistance.

关键词： Stalk strength Maize GWAS Candidate genes Lodging resistance

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Multi-environment QTL mapping of crown root traits in a maize RIL population

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The Crop Journal 2020年第4期8卷 645-654页

作者： Pengcheng Li Yingying Fana Shuangyi Yina Yunyun wanga houmiao wang Yang Xu Zefeng Yang Chenwu Xu Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding Agricultural College of Yangzhou UniversityYangzhou 225009JiangsuChina Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009JiangsuChina Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China Yangzhou UniversityYangzhou 225009JiangsuChina

Crown root traits,including crown root angle(CRA),diameter(CRD),and number(CRN),are major determining factors of root system architecture,which influences crop production.In maize,the genetic mechanisms determining crown root traits in the field are largely unknown.CRA,CRD,and CRN were evaluated in a recombinant inbred line population in three field trials.High phenotypic variation was observed for crown root traits,and all measured traits showed significant genotype–environment interactions.Singleenvironment(SEA)and multi-environment(MEA)quantitative trait locus(QTL)analyses were conducted for CRA,CRD,and CRN.Of 46 QTL detected by SEA,most explained less than 10%of the phenotypic variation,indicating that a large number of minor-effect QTL contributed to the genetic component of these traits.MEA detected 25 QTL associated with CRA,CRD,and CRN,and 2 and 1 QTL were identified with significant QTL-by-environment interaction effects for CRA and CRD,respectively.A total of 26.1%(12/46)of the QTL identified by SEA were also detected by MEA,with many being detected in more than one environment.These findings contribute to our understanding of the phenotypic and genotypic patterns of crown root traits in different environments.The identified environment-specific QTL and stable QTL may be used to improve root traits in maize breeding.

关键词： crown traits maize

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Development and validation of simple sequence repeat markers from Arachis hypogaea transcript sequences

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The Crop Journal 2018年第2期6卷 172-180页

作者： houmiao wang Yong Lei Liying Yan Liyun Wan Yan Cai Zefeng Yang Jianwei Lv Xiaojie Zhang Chenwu Xu Boshou Liao Key Laboratory of Oil Crop Biology of the Ministry of Agriculture CAAS-ICRISAT Joint Laboratory for Groundnut Aflatoxin ManagementOil Crops Research Institute of Chinese Academy of Agricultural SciencesWuhan 430062HubeiChina Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of EducationYangzhou UniversityYangzhou 225009ZhejiangChina

Simple sequence repeats(SSRs) are important molecular markers for assessing genetic diversity in Arachis hypogaea L. and many other crops and constructing genetic linkage maps for important agricultural traits. In this study, 29,357 potential SSRs were identified in 22,806 unigenes assembled from A. hypogaea transcript sequences. Of these unigenes, 1883 and 4103 were annotated and assigned in Kyoto Encyclopedia of Genes and Genomes Orthology and Eukaryotic Orthologous Groups databases, respectively. Among the SSR motifs, mono-(19,065; 64.94%) and trinucleotide(5033; 17.14%) repeats were the most common, and the three most dominant motifs were A/T(18,358; 62.54%), AG/CT(2804;9.55%), and AAG/CTT(1396; 4.76%). Polymerase chain reaction(PCR) primer pairs were designed for 4340 novel SSR markers and 210 new SSRs were validated using 24 A. hypogaea varieties. Of the 210, 191(91%) yielded PCR products, with 37(18%) identifying polymorphisms. The 37 polymorphic SSR markers detected 146 alleles(2–10 alleles per locus), and the average polymorphic information content was 0.403(with a range of 0.077 to0.819). The new SSRs enrich the current marker resources for A. hypogaea and may also be useful for genetic diversity analysis, functional genomics research, and molecular breeding.

关键词： Arachis hypogaea L Molecular marker Polymorphism SSR

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Comparative transcript profiling of resistant and susceptible peanut post-harvest seeds in response to aflatoxin production by Aspergillus flavus

Comparative transcript profiling of resistant and susceptibl...

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江苏省遗传学会2016年学术年会

作者： houmiao wang Yong Lei Liyun Wan Boshou Liao Chenwu Xu Key Laboratory of Oil Crop Biology of the Ministry of Agriculture CAAS-ICRISAT Joint Laboratory for Groundnut Aflatoxin ManagementOil Crops Research Institute of Chinese Academy of Agricultural Sciences Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of EducationYangzhou University

Aflatoxin contamination caused by Aspergillus flavus in peanut（Arachis hypogaea） including in pre-and post-harvest stages seriously affects industry development and human health.Even though resistance to aflatoxin production in post-harvest peanut has been identified,its molecular mechanism has been poorly understood.To understand the mechanism of peanut response to aflatoxin production by A.flavus,RNA-seq was used for global transcriptome profiling of post-harvest seed of resistant（Zhonghua 6） and susceptible（Zhonghua 12） peanut genotypes under the fungus infection and aflatoxin production stress.A total of 128.72 Gb of high-quality bases were generated and assembled into 128,725 unigenes（average length 765 bp）.About 62,352 unigenes（48.43%） were annotated in the NCBI non-redundant protein sequences,NCBI non-redundant nucleotide sequences,Swiss-Prot,KEGG Ortholog,Protein family,Gene Ontology,or eukaryotic Ortholog Groups database and more than 93% of the unigenes were expressed in the samples.Among obtained 30,143 differentially expressed unigenes（DEGs）,842 potential defense-related genes,including nucleotide binding site-leucine-rich repeat proteins,polygalacturonase inhibitor proteins,leucine-rich repeat receptor-like kinases,mitogen-activated protein kinase,transcription factors,ADP-ribosylation factors,pathogenesis-related proteinsand crucial factors of other defense-related pathways,might contribute to peanut response to aflatoxin production.Notably,DEGs involved in phenylpropanoid-derived compounds biosynthetic pathway were induced to higher levels in the resistant genotype than in the susceptible one.Flavonoid,stilbenoid and phenylpropanoid biosynthesis pathways were enriched only in the resistant genotype.This study provided the first comprehensive analysis of transcriptome of post-harvest peanut seeds in response to aflatoxin production,and would contribute to better understanding of molecular interaction between peanut and A.flavus.The data generated in this study would be a valuable resource for geneti

关键词： Arachis hypogaea post-harvest resistance aflatoxin production transcriptome

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高校实验室安全现状分析及管理探析

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科教文汇 2019年第30期 26-27页

作者：王后苗扬州大学农学院

高校实验室是实验教学和科研的重要场所,其安全与师生的人身安全密切相关,且关系到学校乃至整个社会的稳定,因此,实验室安全及其管理具有重要的意义。针对高校实验室存在的普遍问题,结合高校实验室的特色及实际建设情况,通过完善实验室... 详细信息

高校实验室是实验教学和科研的重要场所,其安全与师生的人身安全密切相关,且关系到学校乃至整个社会的稳定,因此,实验室安全及其管理具有重要的意义。针对高校实验室存在的普遍问题,结合高校实验室的特色及实际建设情况,通过完善实验室安全管理制度和安全设施等措施加强安全教育,有利于为师生营造一个安全、和谐的实验环境。

关键词：实验室安全现状分析安全教育管理制度安全设施

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氯酸盐处理对不同基因型玉米苗期性状的影响

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南京农业大学学报 2020年第3期43卷 423-430页

作者：魏杰朱新杰王后苗尹双义杨小艺孙辉李鹏程徐辰武扬州大学江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室/江苏省作物基因组学和分子育种重点实验室/江苏省粮食作物现代产业技术协同创新中心江苏扬州225009

[目的]氯酸盐耐性是鉴定植物氮吸收效率的有效手段,本文旨在探究不同浓度氯酸盐处理对苗期玉米表型、酶活性以及矿质营养元素吸收等方面的影响,并筛选氯酸盐耐性存在差异的自交系。[方法]以6个国内常用玉米自交系Mo17、Dan340、Wu312、... 详细信息

[目的]氯酸盐耐性是鉴定植物氮吸收效率的有效手段,本文旨在探究不同浓度氯酸盐处理对苗期玉米表型、酶活性以及矿质营养元素吸收等方面的影响,并筛选氯酸盐耐性存在差异的自交系。[方法]以6个国内常用玉米自交系Mo17、Dan340、Wu312、Y53、Ye478和B73为研究材料,在温室进行水培试验,分别设置4 mmol·L^-1 KNO3处理(CK)、2 mmol·L^-1 KClO3处理(T1)和4 mmol·L^-1 KClO3处理(T2),持续处理1周后,测定玉米苗期的表型性状、叶片酶活性以及地上部和根系的矿质营养元素含量。[结果]方差分析结果表明:不同浓度氯酸盐处理对玉米自交系表型各个性状均有显著的抑制作用,株高、叶长、SPAD值和地上部干质量4个性状变化最为明显,平均降幅分别为38.96%、37.93%、55.85%和31.58%;而对玉米自交系酶活性影响表现为可溶性蛋白含量(Cpr)显著增加,谷氨酸合成酶活性(GOGAT)显著下降;氯酸盐处理抑制玉米对氮和锌的吸收。[结论]株高、叶长、SPAD值和地上部干质量对氯酸盐处理的响应可以作为筛选氯酸盐耐性自交系的指标。以地上部干质量对氯酸盐处理的响应为筛选指标,B73、Y53和Wu312为氯酸盐敏感性材料,Mo17、Ye478和Dan340为氯酸盐耐性材料。本研究为玉米氮吸收的表型鉴定提供新思路。

关键词：玉米氯酸盐表型酶活性矿质营养元素

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花生收获前黄曲霉毒素污染抗性及其与花生安全贮藏关系的分析

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扬州大学学报（农业与生命科学版） 2018年第3期39卷 58-62,90页

作者：王后苗潘婷魏杰雷永吕建伟成良强徐辰武廖伯寿扬州大学农学院/植物功能基因组学教育部重点实验室/江苏省作物遗传生理重点实验室/江苏省作物基因组学与分子育种重点实验室/江苏省粮食作物现代产业技术协同创新中心江苏扬州225009 中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室武汉430062

为探究花生收获前黄曲霉毒素污染抗性及其与花生安全贮藏关系,测定具有收获后产毒抗性花生品系的收获前黄曲霉菌侵染率和毒素含量,分析收获前、后黄曲霉毒素污染抗性与花生安全贮藏的关系。结果表明:不同花生品种(系)在生长期间抵抗黄... 详细信息

为探究花生收获前黄曲霉毒素污染抗性及其与花生安全贮藏关系,测定具有收获后产毒抗性花生品系的收获前黄曲霉菌侵染率和毒素含量,分析收获前、后黄曲霉毒素污染抗性与花生安全贮藏的关系。结果表明:不同花生品种(系)在生长期间抵抗黄曲霉菌侵染或产毒能力不同,中花6号和J091收获前黄曲霉菌侵染率较高,但产毒量与其他材料相当,表明其具有一定的收获前产毒抗性。收获前抗性与收获后抗性无相关性,但收获前黄曲霉菌侵染率与贮藏花生的黄曲霉毒素含量显著正相关。因此,控制花生收获前的黄曲霉菌侵染,尤其是通过遗传改良选育兼备收获前、后抗性的花生新品种,是降低黄曲霉毒素污染,保证花生食用安全性的有效措施。

关键词：花生黄曲霉毒素抗性安全贮藏

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