Genome-wide association study identifies novel candidate loci or genes affecting stalk strength in maize

作     者：Shuhui Xu Xiao Tang Xiaomin Zhang Houmiao Wang Weidong Ji Chenwu Xu Zefeng Yang Pengcheng Li Shuhui Xu;Xiao Tang;Xiaomin Zhang;Houmiao Wang;Weidong Ji;Chenwu Xu;Zefeng Yang;Pengcheng Li

作者机构：Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular BreedingYangzhou UniversityYangzhou 225009JiangsuChina Jiangsu Co-Innovation Center for Modern Production Technology of Grain CropsYangzhou UniversityYangzhou 225009JiangsuChina National Maize Improvement Center of ChinaChina Agricultural UniversityBeijing 100193China State Key Laboratory of Crop Stress Adaptation and ImprovementKey Laboratory of Plant Stress BiologySchool of Life SciencesHenan UniversityKaifeng 475001HenanChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2023年第11卷第1期

页      面：220-227页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(31972487,31902101,32172009 and 32061143030) the Innovative Research Team of Universities in Jiangsu Province,the Science and Technology Development Plan Project of Henan Province(212102110152) the High-end Talent Project of Yangzhou University the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD) 

主　　题：Stalk strength Maize GWAS Candidate genes Lodging resistance 

摘      要：Stalk strength increases resistance to stalk lodging,which causes maize(Zea mays L.)production losses *** genetic mechanisms regulating stalk strength remain *** this study,three stalk strength-related traits(rind penetrometer resistance,stalk crushing strength,and stalk bending strength)and four plant architecture traits(plant height,ear height,stem diameter,stem length)were measured in three field *** phenotypic variation was detected for these traits.A genome-wide association study(GWAS)was conducted using general and mixed linear models and 372,331 single-nucleotide polymorphisms(SNPs).A total of 94 quantitative trait loci including 241 SNPs were *** combining the GWAS data with public gene expression data,56 candidate genes within 50 kb of the significant SNPs were identified,including genes encoding flavonol synthase(GRMZM2G069298,ZmFLS2),nitrate reductase(GRMZM5G878558,ZmNR2),glucose-1-phosphate adenylyltransferase(GRMZM2G027955),and laccase(GRMZM2G447271).Resequencing GRMZM2G069298 and GRMZM5G878558 in all tested lines revealed respectively 47 and 2 variants associated with *** of the RPR of the zmnr2EMS mutant and the wild-type plant under high-and low-nitrogen conditions verified the GRMZM5G878558 *** findings may be useful for clarifying the genetic basis of stalk *** identified candidate genes and variants may be useful for the genetic improvement of maize lodging resistance.