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人脐带间充质干细胞共培养联合人参皂苷Rg1对心力衰竭细胞模型的影响

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中国组织工程研究 2025年第31期29卷 6625-6633页

作者：任疏桐郝苗刘越侯平全娟花辽宁中医药大学辽宁省沈阳市110000 沈阳市第四人民医院中医科辽宁省沈阳市110031 沈阳医学院中医教研室辽宁省沈阳市110031 辽宁中医药大学附属医院辽宁省沈阳市110000 广东医科大学附属医院广东省湛江市524000

背景:目前,如何促进细胞扩增、减少细胞损失、提高细胞归巢率、减少细胞凋亡是人脐带间充质干细胞临床前研究的主要问题。人参皂苷Rg1可在体外或体内促进间充质干细胞在不同微环境中的增殖、分化,其可能是提高人脐带间充质干细胞移植效... 详细信息

背景:目前,如何促进细胞扩增、减少细胞损失、提高细胞归巢率、减少细胞凋亡是人脐带间充质干细胞临床前研究的主要问题。人参皂苷Rg1可在体外或体内促进间充质干细胞在不同微环境中的增殖、分化,其可能是提高人脐带间充质干细胞移植效率的候选药物。目的:探讨人脐带间充质干细胞共培养联合人参皂苷Rg1对戊巴比妥钠诱导的心衰细胞模型的影响。方法:将H9C2细胞分为5组:对照组、戊巴比妥钠组、人脐带间充质干细胞组、人参皂苷Rg1组、人脐带间充质干细胞+人参皂苷Rg1组。对照组H9C2细胞用正常DMEM培养基培养24 h,其他组H9C2细胞用含0.8%戊巴比妥钠的DMEM培养基培养7 min构建心衰细胞模型。建模后,分别用人脐带间充质干细胞、人参皂苷Rg1单独或共同处理。采用CCK-8法和EdU染色检测细胞增殖情况,TUNEL法检测细胞凋亡情况,按试剂盒说明检测Na^(+)-K^(+)-ATP酶和Ca^(2+)-Mg^(2+)-ATP酶活性,ELISA测定上清液中肿瘤坏死因子α、白细胞介素1β和白细胞介素6水平,RT-qPCR测定细胞中肿瘤坏死因子α、白细胞介素1β、白细胞介素6、Bax和Bcl-2 mRNA表达,Western blot测定细胞中Bax、Bcl-2、Toll样受体4、p65和p-p65蛋白表达。结果与结论:①与戊巴比妥钠组比较,人脐带间充质干细胞组、人参皂苷Rg1组和人脐带间充质干细胞+人参皂苷Rg1组H9C2细胞活力和EdU阳性率升高,TUNEL阳性率以及Bax mRNA和蛋白表达降低,Bcl-2 mRNA和蛋白表达升高,Na^(+)-K^(+)-ATP酶活性降低,Ca^(2+)-Mg^(2+)-ATP酶活性升高,H9C2细胞上清液中肿瘤坏死因子α、白细胞介素1β和白细胞介素6水平降低,H9C2细胞中肿瘤坏死因子α、白细胞介素1β和白细胞介素6 mRNA表达降低,Toll样受体4和p-p65蛋白表达降低,差异均有显著性意义(P<0.05);②与人脐带间充质干细胞组和人参皂苷Rg1组比较,人脐带间充质干细胞+人参皂苷Rg1组上述指标进一步改善(P<0.05)。结果表明:人脐带间充质干细胞联合人参皂苷Rg1提高了戊巴比妥钠诱导的心衰细胞活力,并抑制了Toll样受体4/核因子κB通路介导的炎症。

关键词：人脐带间充质干细胞 H9C2细胞心力衰竭人参皂苷Rg1 戊巴比妥钠 Toll样受体4 核因子κB

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Multi-functional Hollow Structures for Intelligent Drug Delivery

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Chemical Research in Chinese Universities 2024年第3期40卷 394-412页

作者： hou ping YANG Nailiang WANG Dan State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190P.R.China School of Chemical Engineering University of Chinese Academy of SciencesBeijing 100049P.R.China Key Laboratory of Biopharmaceutical Preparation and Delivery Chinese Academy of SciencesBeijing 100049P.R.China

Multi-fountional hollow structures have emerged as promising platforms for intelligent drug delivery due to their unique properties,such as high loading capacities and programmed drug *** particular,hollow multishell structures(HoMSs)with multilevel shell and space can regulate the molecular-level interaction between drugs and materials,so as to achieve the temporal-spatial order and sequential release of *** anisotropic hollow structures can control the drug diffusion process by inducing the macroscopic interface flow through autonomous movement,realizing the targeted drug transport and *** this paper,a key focus will be HoMSs with their temporal-ordered architectures and anisotropic hollow carriers with directional *** synthesis mechanisms,structure-property relationships,smartly programmed drug delivery and biomedical applications will be discussed,providing insights into designing next-generation intelligent drug carriers.

关键词： Hollow multishell structure Anisotropic hollow structure Intelligent drug carrier Temporal-spatial order Directional movement

The association between matrix metalloprotease-9 gene polymorphisms and primary angle-closure glaucoma in a Chinese Han population

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International Journal of Ophthalmology(English edition) 2014年第3期7卷 397-402页

作者： Xiao-Jin Gao Sheng-ping hou ping-Hua Li Department of Ophthalmology Chengdu First People's Hospital Chongqing Key Laboratory of Ophthalmology Department of Ophthalmology the First Affiliated Hospital of Chongqing Medical University

AIM:To examine the association between the single nucleotide polymorphisms (SNPs)of matrix metalloprotease-9 (MMP-9) gene and primary angleclosure glaucoma(PACG)in a Chinese Han ***:DNA samples were extracted from peripheral-blood mononuclear cells of 214 PACG patients and 224 healthy *** of rs3918249,rs3918254,rs17577 and rs3787268 in MMP-9was performed using polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP)analysis and the direct sequencing *** association between these genetic polymorphisms and risk of PACG was estimated by χ2 ***:The distributions of rs3918249,rs3918254,rs17577 and rs3787268 genotypes among cases and healthy controls were compatible with that from HardyWeinberg equilibrium(HWE,P>0.05).The increased frequency of CC and CT genotypes of rs3918254 were observed in PACG patients compared to healthy controls[P=0.006,P corrected(Pcorr)=0.048].The haplotype analysis showed that the CCGG haplotype was nominal associated with PACG(P=0.015),however,the significant was lost when the Bonferroni correction was used(Pcorr=0.105).CONCLUSION:Our results revealed that rs3918254 in MMP-9 may be a susceptible locus to PACG in China,people with the CC and CT genotypes of rs3918254 are more susceptible to *** susceptibility to PACG inChinese Han patients may be not influenced by SNPs rs3918249,rs3787268 and rs17577 in MMP-9.

关键词： matrix metalloprotease-9 gene primary angle-closure glaucoma single nucleotide polymorphisms

Numerical analysis and optimization of the inlet stage of a multi-stage high pressure compressor

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航空动力学报 2007年第8期22卷 1356-1364页

作者： XU Quan-yong hou An-ping XIONG Jin-song Zhou Sheng National Key Laboratory on Aero-engines School of Jet Propulsion Beijing University of Aeronautics and Astronautics Beijing 100083

An opti mization process is used to redesign blades of a high-pressure *** artificial neural network (ANN) method is coupled to Navier-Stokes solvers and is applied to three different redesigns.A newrotor blade of a transonic compressor is designed by modifying thick,stacking line andinlet angle using a 3Dapproach,with a significant efficiencyi mprovement at the design *** off-design behavior of this new compressor is also checked afterwards,which shows that the whole performance of the inlet stage is improved over a wide range of mass *** losses are reduced,proving the good performance of the opti *** whole results indicate that the opti mization method can find i mproved design and can be integrated in a design procedure.

关键词：数学分析最优化航天推进系统高压压缩机人工神经网络叶栅

Graphs Whose Critical Groups Have Larger Rank

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Acta Mathematica Sinica,English Series 2011年第9期27卷 1663-1670页

作者： Yao ping hou Wai Chee SHIU Wai Hong CHAN Departement of Mathematics Hu'nan Normal University Changsha 410081 P. R. China Department of Mathematics Hong Kong Baptist University Kowloon Tong Hong Kong P. R. China

The critical group C（G） of a graph G is a refinement of the number of spanning trees of the graph and is closely connected with the Laplacian matrix. Let r（G） be the minimum number of generators （i.e., the rank） of the group C（G） and β（G） be the number of independent cycles of G. In this paper, some forbidden induced subgraphs axe given for r（G） = n - 3 and all graphs with r（G） = j3（G） = n - 3 are characterized

关键词： Critical group of a graph Laplacian matrix Smith normal form

W, Mo, Co Co-diffusion on Steel with Different Carbon Content

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材料热处理学报 2004年第05B期25卷 688-691页

作者： ZHANGYan-mei LIZhong-hou LIUYan-ping XUZhong ResearchInstituteofSurfaceEngineering TaiyuanUniversityofTechnologyTaiyuanChina

Tungsten, molybdenum and cobalt were co-diffused into the surface of ingot, 20 steel, 45 steel and T8 steel by a double glow plasma surface alloying technique. Surface alloyed layers formed on ingot and 20 steel are composed of -Fe +(Fe,Co)7(W,Mo)6, which possess the same strong age-hardening ability as the metallurgical age-hardened high speed steel, the maximum hardness can reach 1100 HV0.o25 after ageing treatment. Surface alloyed layers formed on 45 steel and T8 steel are composed of M6C, hardness of which are over 1100-1400 HV0.o25- Alloyed layers formed on ingot and 20 steel possess good toughness. Alloyed layer of carbide type exhibits slightly brittleness.

关键词：碳含量表面铸合碳钢硬度脆性钼钴共扩散

Paraventricular Thalamus as A Major Thalamic Structure for Wake Control

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Neuroscience Bulletin 2019年第5期35卷 946-948页

作者： Yu-Feng Shao Jian-Sheng Lin Yi-ping hou Departments of Neuroscience Anatomy Histology and Embryology Key Laboratory of Preclinical Study for New Drugs of Gansu Province School of Basic Medical Sciences Lanzhou University Lanzhou 730000 China Integrative Physiology of the Brain Arousal Systems Lyon Neuroscience Research Center Lyon 69373 France

The thalamus is the gate of the cerebral cortex, the ultimate target for the neural networks controlling behavioral states and cognitive functions. According to the reticular theory initially proposed by Moruzzi and Magoun, excitatory inputs from large reticular zones of the brainstem via widespread intra- and extra-thalamocortical systems finally activate the cerebral cortex to cause generalized cortical activation and wakefulness [1]. This theory proposes a central relay role to the thalamus for cortical activation as supported by early studies using neurodegeneration techniques and by the elegant work of Steriade’s group and other investigators illustrating the electrophysiological mechanisms of the thalamocortical system at the cellular level during wakefulness, rapid eye-movement sleep (REMs) and non-REM sleep (NREMs)[2].

关键词： Paraventricular Thalamus Thalamic Structure Wake Control

Effects and Mechanism of Action of Artemisinin on Mitochondria of Plasmodium berghei

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Chinese Journal of Integrative Medicine 2020年第4期26卷 277-282页

作者： hou Hong-ping ZHANG Guang-ping MA Li-na SU ping ZHANG Zhong-xiu DAI Bao-qiang YE Zu-guang Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing(100700)China

Objective:To study the antimalarial effects and mechanisms of artemisinin(Qinghaosu in Chinese,QHS)on mitochondria in mice infected with Plasmodium ***:A total of 108 C57 mice infected with Plasmodium berghei were randomly divided into 3 groups by weight:the control group,200 and 400 mg/kg QHS *** two QHS treatment groups were further divided into 4 sub-groups with 12 animals each time according to the treatment time,0.5,1,2,and 4 *** saline was intragastrically(i.g.)administered to the control *** other two groups received different doses of QHS by *** were treated once with QHS for different detection time as follows:0.5,1,2,and 4 *** mitochondrial energy metabolism,oxidative damage,membrane potential,and membrane permeability and other indexes were ***:After administration of 200 and 400 mg/kg QHS,adenosine triphosphate(ATP)levels in Plasmodium and its mitochondria were reduced(P<0.05),the levels of reactive oxygen species(ROS)and malondialdehyde(MDA)were in creased(P<0.05),and the activity of superoxide dismutase(SOD)was also increased(P<0.05).At the same time,the membrane potential of the mitochondria was reduced and the degree to which the membrane permeability transition pore was ope ned was irreversibly in creased(P<0.05).Conclusions:Mitoch on dria in Plasmodium were the targets of QHS,which can adversely affect mitochondrial energy metabolism,oxidative damage,membrane potential,and membrane opening,and ultimately exert an antimalarial effect.

关键词： Qinghaosu Clinese medicine Plasmodium mitochondria antimalarial effect

Enhanced Impurity-Free Intermixing Bandgap Engineering for InP-Based Photonic Integrated Circuits

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Chinese Physics Letters 2014年第4期31卷 64-67页

作者： CUI Xiao ZHANG Can LIANG Song ZHU Hong-Liang hou Lian-ping Key Laboratory of Semiconductor Materials Science Institute of Semiconductors Chinese Academy of Sciences Beijing 100083 School of Engineering University of Glasgow Oakfield Avenue Glasgow G12 8LT UK

hnpurity-free intermixing of InGaAsP multiple quantum wells （MQW） using sputtering Cu/Si02 layers followed by rapid thermal processing （RTP） is demonstrated. The bandgap energy could be modulated by varying the sputtering power and time of Cu, RTP temperature and time to satisfy the demands for lasers, modulators, pho- todetector, and passive waveguides for the photonic integrated circuits with a simple procedure. The blueshift of the bandgap wavelength of MQW is experimentally investigated on different sputtering and annealing conditions. It is obvious that the introduction of the Cu layer could increase the blueshift more greatly than the common impurity free vacancy disordering technique. A maximum bandgap blueshift of 172nm is realized with an anneal- ing condition of 750~C and 200s. The improved technique is promising for the fabrication of the active/passive optoeleetronic components on a single wafer with simple process and low cost.

关键词： Circuits Enhanced sputtering