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基于文献计量的乳酸菌细菌素研究进展分析

食品工业科技 2024年第9期45卷 378-388页

作者：甄子辰刘阳王珊珊路宏朝王令张涛陕西理工大学生物科学与工程学院陕西汉中723001 秦巴生物资源与生态环境省部共建国家重点实验室(培育) 陕西汉中723001 陕南秦巴山区生物资源综合开发协同创新中心陕西汉中723001 秦巴特色肉制品质量提升与安全控制陕西省高校工程研究中心陕西汉中723001 陕西省四主体一联合镇巴腊肉校企联合研究中心陕西汉中723001 陕西省资源生物重点实验室陕西汉中723001

乳酸菌细菌素是乳酸菌在代谢过程中产生的一类具有抑菌活性的天然蛋白质或多肽类物质,具有无抗药性、可生物降解、抑菌效果好等优点,在食品、医药、饲料领域应用前景广阔。本文基于文献计量学方法,检索2000~2023年CNKI数据库和Web of Sc... 详细信息

乳酸菌细菌素是乳酸菌在代谢过程中产生的一类具有抑菌活性的天然蛋白质或多肽类物质,具有无抗药性、可生物降解、抑菌效果好等优点,在食品、医药、饲料领域应用前景广阔。本文基于文献计量学方法,检索2000~2023年CNKI数据库和Web of Science(WOS)核心合集有关乳酸菌细菌素领域的文献,得到627篇中文文献和2543篇英文文献。使用CiteSpace软件从年度发文量,发文国家、作者、机构和期刊,高被引文献,关键词共现聚类等角度分析该领域的研究现状和热点。结果显示:2000~2022年发文量总体呈上升的趋势。西班牙、印度、中国的发文量在国家排行前三。CNKI数据库与WOS数据库发文量最高的作者分别是中国农业大学李平兰和巴西圣保罗大学Todorov SD。发文量最高的期刊分别是《食品工业科技》与《Journal of Appled Micbiogy》。CNKI数据库中关键词分析可以看出,文章多是从不同食品样本中分离、筛选、鉴定获得产细菌素的乳酸菌,进一步评价细菌素的抑菌活性,并探讨其在食品行业中的应用;突现词分析表明细菌素抑菌机制、细菌素与益生菌的联系是目前研究热点。WOS数据库中关键词“胃肠道”、“基因特征”、“单增核李斯特菌”、“抑菌活性”出现频次较高,主要热点是抑制抗生素耐药细菌的乳酸菌细菌素的挖掘及其基因特征的解析。通过文献综合分析,为我国科研人员从事相关研究和预测行业未来发展趋势提供参考和帮助。

关键词：细菌素乳酸菌文献计量 CiteSpace

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兔肉脂肪酸代谢相关基因的转录组数据分析

现代食品 2024年第17期30卷 184-191页

作者：高航董启启姬正伟程佳路宏朝卢军陕西省汉中市市场监督管理局陕西汉中723001 陕西理工大学生物科学与工程学院陕西汉中723001 秦巴特色肉制品质量提升与安全控制陕西省高校工程研究中心陕西汉中723001

脂肪酸组成是影响肉制品品质的关键因素。为了研究兔肉脂肪酸形成的功能基因的表达机制,在统计不同品种和部位兔肉脂肪酸含量的基础上,利用转录组学数据系统分析伊拉兔腓肠肌脂肪酸代谢的相关基因的表达模式,并进行了功能和通路富集。... 详细信息

脂肪酸组成是影响肉制品品质的关键因素。为了研究兔肉脂肪酸形成的功能基因的表达机制,在统计不同品种和部位兔肉脂肪酸含量的基础上,利用转录组学数据系统分析伊拉兔腓肠肌脂肪酸代谢的相关基因的表达模式,并进行了功能和通路富集。结果表明,不同兔肉品种间的脂肪酸含量差异较大,而同一品种不同部位之间的脂肪酸含量无明显差异。在伊拉兔腓肠肌中,脂肪酸代谢基因中的脂蛋白脂肪酶(Lipoprotein Lipase,LPL)、脂肪酸结合蛋白4(Fatty Acid Binding Protein 4,FABP4)和磷酸腺苷激活蛋白激酶(Adenosine Monophosphate Activated Protein Kinase,AMPK)调节亚基γ3(Gamma 3 Non-Catalytic Subunit,PRKAG3)等蛋白的基因主要通过AMPK信号通路参与调节其脂肪酸组成。经实时定量聚合酶链式反应方法验证的基因也基本符合转录组学的表达趋势。本次研究结果可为兔肉风味形成机制的明确和肉质改良提供理论依据。

关键词：兔肉脂肪酸转录组

Profiling of miRNAs in porcine Sertoli cells

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Journal of Animal Science and Biotechnology 2021年第1期12卷 236-249页

作者： Xiaoxu Chen Yi Zheng Xueliang Li Qiang Gao Tongying Feng Pengfei Zhang Mingzhi Liao Xiu’e Tian hongzhao lu Wenxian Zeng College of Biological Science and Engineering Shaanxi University of TechnologyHanzhong 723001China Key Laboratory of Animal Genetics Breeding and Reproduction of the Ministry of AgricultureCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100ShaanxiChina College of Life Science Northwest A&F UniversityYangling 712100ShaanxiChina.

Background: Sertoli cells(SCs) create a specialized environment to support and dictate ***(miRNAs), a kind of ~ 22 nt small noncoding RNAs, have been reported to be highly abundant in mouse SCs and play critical roles in spermatogenesis. However, the miRNAs of porcine SCs remain largely ***: We isolated porcine SCs and conducted small RNA sequencing. By comparing miRNAs in germ cells, we systematically analyzed the miRNA expression pattern of porcine SCs. We screened the highly enriched SC miRNAs and predicted their functions by Gene Ontology analysis. The dual luciferase assay was used to elucidate the regulation of tumor necrosis factor receptor(TNFR)-associated factor 3(TRAF3) by ***: The analysis showed that 18 miRNAs were highly expressed in SCs and 15 miRNAs were highly expressed in germ cells. These miRNAs were predicted to mediate SC and germ cell functions. In addition, ssc-miR-149 played critical roles in SCs by targeting ***: Our findings provide novel insights into the miRNA expression pattern and their regulatory roles of porcine SCs.

关键词： MicroRNAs Pig Sertoli cell Spermatogenesis TRAF3

Single-cell RNA-seq analysis of testicular somatic cell development in pigs

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Journal of Genetics and Genomics 2022年第11期49卷 1016-1028页

作者： Lingkai Zhang Ming Guo Zidong Liu Ruifang Liu Yi Zheng Taiyong Yu Yinghua Lv hongzhao lu Wenxian Zeng Tao Zhang Chuanying Pan College of Animal Science and Technology Northwest A&F UniversityYanglingShaanxi 712100China College of Chemistry and Pharmacy Northwest A&F UniversityYanglingShaanxi 712100China School of Biological Science and Engineering Shaanxi University of TechnologyHanzhongShaanxi 723001China

Spermatogenesis is the process by which diploid male germ cells propagate and differentiate into haploid flagellated *** highly complicated process is dependent on testicular somatic cells *** the role of these somatic cells in spermatogenesis is relatively well established,knowledge about their development and maturation,particularly at the molecular level,is *** this study,we profiled the testicular single-cell transcriptomes of Guanzhong black pigs at the age of 7,30,60,90,and 150 *** types of Sertoli cells,five types of Leydig cells,and four types of peritubular myoid cells were *** analysis revealed the changes in proliferation levels and marker gene expressions,and the prion-like protein gene(PRND)was identified as a novel marker for Sertoli ***,integrated analyses of porcine and human datasets revealed similarities between human and pig testicular somatic ***,the data obtained in this study contribute to the understanding of testicular development in pigs as a model species.

关键词： Pigs scRNA-seq Testis somatic cells Puberty

FGF13通过ROS介导抑制乳腺癌细胞MCF7凋亡

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生物技术 2024年第2期34卷 210-216,236页

作者：莫莎唐铖铖刘怡路宏朝马海东曾文先陕西理工大学生物科学与工程学院陕西汉中723000 汉中职业技术学院医学技术学院陕西汉中723000

[目的]探究成纤维细胞生长因子13(Fibroblast growth factor 13,FGF13)对乳腺癌细胞MCF7凋亡的调控作用。[方法]Western Blotting和免疫组织化学染色检测FGF13的表达;构建干扰和超表达FGF13的MCF7细胞株;TUNEL法和Western Blotting检测细胞凋亡;DCFH-DA荧光探针法检测细胞内活性氧(Reactive oxygen species, ROS)。[结果]与正常乳腺细胞相比,FGF13在乳腺癌MCF7细胞中表达升高(0.16±0.01 vs 3.89±0.23,P<0.001)。干扰FGF13后,MCF7细胞凋亡率升高(2.20%±0.10%vs 10.79%±0.69%,P<0.001),促凋亡蛋白Bax(0.99±0.02 vs 2.56±0.02)、Caspase 3(1.00±0.01 vs 1.70±0.01)和p53(0.98±0.01 vs 2.22±0.03)的表达上调(P<0.001),而抗凋亡蛋白Bcl-xl(0.99±0.01 vs 0.29±0.01)、Bcl-2(0.99±0.01 vs 0.27±0.01)的表达下调(P<0.001)。相反,超表达FGF13后,细胞凋亡率和促凋亡蛋白的表达均降低(P<0.001),而抗凋亡蛋白的表达均升高(P<0.001)。而且,干扰FGF13的表达引起细胞内ROS水平升高(1 552.00±51.37 vs 2 714.00±55.19,P<0.001),而超表达则导致ROS减少(2 102.83±65.29 vs 1 111.67±63.53,P<0.001)。[结论]FGF13可能通过ROS介导抑制乳腺癌MCF7细胞凋亡。

关键词：乳腺癌细胞凋亡成纤维细胞生长因子13 活性氧抗凋亡蛋白促凋亡蛋白基因表达半胱天冬酶

Transcriptome-wide Dynamics of m^(6)A mRNA Methylation During Porcine Spermatogenesis

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Genomics, Proteomics & Bioinformatics 2023年第4期21卷 729-741页

作者： Zidong Liu Xiaoxu Chen Pengfei Zhang Fuyuan Li Lingkai Zhang Xueliang Li Tao Huang Yi Zheng Taiyong Yu Tao Zhang Wenxian Zeng hongzhao lu Yinghua Lv Key Laboratory for Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China School of Biological Science and Engineering Shaanxi University of TechnologyHanzhong 723001China College of Chemistry and Pharmacy Northwest A&F UniversityYangling 712100China

Spermatogenesis is a continual process that occurs in the testes,in which diploid spermatogonial stem cells(SSCs)differentiate and generate haploid *** highly efficient and intricate process is orchestrated at multiple levels.N^(6)-methyladenosine(m^(6)A),an epigenetic modification prevalent in mRNAs,is implicated in the transcriptional regulation during ***,the dynamics of m^(6)A modification in non-rodent mammalian species remains ***,we systematically investigated the profile and role of m^(6)A during spermatogenesis in *** analyzing the transcriptomic distribution of m^(6)A in spermatogonia,spermatocytes,and round spermatids,we identified a globally conserved m^(6)A pattern between porcine and murine genes with spermatogenic *** found that m^(6)A was enriched in a group of genes that specifically encode the metabolic enzymes and *** addition,transcriptomes in porcine male germ cells could be subjected to the m^(6)A *** data show that m^(6)A plays the regulatory roles during spermatogenesis in pigs,which is similar to that in *** of this point are three genes(SETDB1,FOXO1,and FOXO3)that are crucial to the determination of the fate of *** the best of our knowledge,this study for the first time uncovers the expression profile and role of m^(6)A during spermatogenesis in large animals and provides insights into the intricate transcriptional regulation underlying the lifelong male fertility in non-rodent mammalian species.

关键词： N^(6)-methyladenosine Spermatogonial stem cell Spermatogenesis SETDB1 Pig

基于文献计量的肉制品微生物研究现状和热点分析

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食品工业科技 2023年第21期44卷 71-82页

作者：宁波王令路宏朝张涛陕西理工大学生物科学与工程学院陕西汉中723001 陕西理工大学秦巴生物资源与生态环境省部共建国家重点实验室(培育) 陕西汉中723001 秦巴特色肉制品质量提升与安全控制陕西省高校工程研究中心陕西汉中723001 陕西省“四主体一联合”镇巴腊肉校企联合研究中心陕西汉中723001 陕南秦巴山区生物资源综合开发协同创新中心陕西汉中723001

为了探究肉制品微生物的研究进展、热点和未来发展趋势,本文基于文献计量学方法,利用CiteSpace知识图谱分析工具,结合1980~2022年中国知网(CNKI)学术期刊数据库和1977~2022年Web of Science(WOS)核心合集数据库对肉制品微生物领域842篇... 详细信息

为了探究肉制品微生物的研究进展、热点和未来发展趋势,本文基于文献计量学方法,利用CiteSpace知识图谱分析工具,结合1980~2022年中国知网(CNKI)学术期刊数据库和1977~2022年Web of Science(WOS)核心合集数据库对肉制品微生物领域842篇中文文献和1530篇英文文献进行计量统计和可视化分析。从年度发文量,核心文献,核心发文国家、作者、机构以及期刊分布,关键词共现聚类等角度,阐述了该领域的研究现状和热点。结果显示,有关肉制品微生物的研究,国内外发文量均呈现波动上升的趋势且外文文献量增长更为显著,外文文献的被引频次远高于中文论文;发文国家主要以美国、西班牙、巴西和中国等为主力军;研究机构主要为高校和科研院所,如西班牙高等科研理事会(CSIC)、美国农业科学研究院(ARS)、江南大学等;全球研究热点主要集中于微生物对肉制品风味品质和理化性质的影响、微生物在肉制品生产过程中参与代谢的机理以及食品安全性等方面。本文通过综合分析,为我国科研人员从事相关研究和预测行业未来发展趋势提供参考和帮助。

关键词： CiteSpace 肉制品微生物研究热点文献计量分析

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Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis

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Journal of Animal Science and Biotechnology 2022年第2期13卷 458-472页

作者： Lingkai Zhang Fuyuan Li Peipei Lei Ming Guo Ruifang Liu Ling Wang Taiyong Yu Yinghua Lv Tao Zhang Wenxian Zeng hongzhao lu Yi Zheng Key Laboratory for Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100ShaanxiChina School of Biological Science and Engineering Shaanxi University of TechnologyHanzhong 723001ShaanxiChina College of Chemistry and Pharmacy Northwest A&F UniversityYangling 712100ShaanxiChina

Background:Spermatogenesis is the process by which male gametes are formed from spermatogonial stem cells and it is essential for the reliable transmission of genetic information between *** date,the dynamic transcriptional changes of defined populations of male germ cells in pigs have not been ***:To characterize the atlas of porcine spermatogenesis,we profiled the transcriptomes of~16,966 testicular cells from a 150-day-old pig testis through single-cell RNA-sequencing(scRNA-seq).The scRNA-seq analysis identified spermatogonia,spermatocytes,spermatids and three somatic cell types in porcine *** functional enrichment analysis demonstrated that these cell types played diverse roles in porcine *** accuracy of the defined porcine germ cell types was further validated by comparing the data from scRNA-seq with those from bulk *** we delineated four distinct spermatogonial subsets,we further identified CD99 and PODXL2 as novel cell surface markers for undifferentiated and differentiating spermatogonia,***:The present study has for the first time analyzed the transcriptome of male germ cells and somatic cells in porcine testes through *** subsets of spermatogonia were identified and two novel cell surface markers were discovered,which would be helpful for studies on spermatogonial differentiation in *** datasets offer valuable information on porcine spermatogenesis,and pave the way for identification of key molecular markers involved in development of male germ cells.

关键词： Marker Pig scRNA-seq Spermatogenesis

裸眼识别二氧化硫的长波长荧光探针的合成及应用

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分析试验室 2022年第7期41卷 794-800页

作者：曹小燕路宏朝田光辉任传清陕西省催化基础与应用重点实验室陕西理工大学化学与环境科学学院汉中723001 陕西理工大学生物科学与工程学院汉中723001

基于迈克尔(Michael)加成反应机理,设计合成了一种简单的亚硫酸盐荧光探针(E)-3-乙基-1,1-二甲基-2-(2-(5-(4-硝基苯基)呋喃-2-基)乙烯基)-^(1)H-苯并[e]吲哚碘化物(NFBI),核磁共振氢谱(^(1)H NMR)、核磁共振碳谱(^(13)C NMR)和高分辨... 详细信息

基于迈克尔(Michael)加成反应机理,设计合成了一种简单的亚硫酸盐荧光探针(E)-3-乙基-1,1-二甲基-2-(2-(5-(4-硝基苯基)呋喃-2-基)乙烯基)-^(1)H-苯并[e]吲哚碘化物(NFBI),核磁共振氢谱(^(1)H NMR)、核磁共振碳谱(^(13)C NMR)和高分辨质谱图(HRMS)验证其结构。NFBI在磷酸盐:N,N-二甲基甲酰胺(PBS:DMF,1∶1,V∶V,pH 7.4)溶液中通过荧光信号“on-off”的变化快速识别SO_(2),且颜色由橙红色变为无色,借助^(1)H NMR和HRMS,研究了探针NFBI识别SO_(2)的机理。结果表明,探针NFBI对SO_(2)具有较快的响应速度(30 s)、较高的灵敏度和良好的选择性。更重要的是,探针NFBI具有低毒性和良好的膜通透性,能够可视化检测活细胞和斑马鱼中的SO_(2)。

关键词：荧光探针二氧化硫裸眼识别生物成像