Single-cell RNA-seq analysis of testicular somatic cell development in pigs

作     者：Lingkai Zhang Ming Guo Zidong Liu Ruifang Liu Yi Zheng Taiyong Yu Yinghua Lv Hongzhao Lu Wenxian Zeng Tao Zhang Chuanying Pan Lingkai Zhang;Ming Guo;Zidong Liu;Ruifang Liu;Yi Zheng;Taiyong Yu;Yinghua Lv;Hongzhao Lu;Wenxian Zeng;Tao Zhang;Chuanying Pan

作者机构：College of Animal Science and TechnologyNorthwest A&F UniversityYanglingShaanxi 712100China College of Chemistry and PharmacyNorthwest A&F UniversityYanglingShaanxi 712100China School of Biological Science and EngineeringShaanxi University of TechnologyHanzhongShaanxi 723001China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2022年第49卷第11期

页      面：1016-1028页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：supported in part by the National Natural Science Foundation of China(31772605) Research Project of Shaanxi Science and Technology Department(2020NY-003)to Tao Zhang 

主　　题：Pigs scRNA-seq Testis somatic cells Puberty 

摘      要：Spermatogenesis is the process by which diploid male germ cells propagate and differentiate into haploid flagellated *** highly complicated process is dependent on testicular somatic cells *** the role of these somatic cells in spermatogenesis is relatively well established,knowledge about their development and maturation,particularly at the molecular level,is *** this study,we profiled the testicular single-cell transcriptomes of Guanzhong black pigs at the age of 7,30,60,90,and 150 *** types of Sertoli cells,five types of Leydig cells,and four types of peritubular myoid cells were *** analysis revealed the changes in proliferation levels and marker gene expressions,and the prion-like protein gene(PRND)was identified as a novel marker for Sertoli ***,integrated analyses of porcine and human datasets revealed similarities between human and pig testicular somatic ***,the data obtained in this study contribute to the understanding of testicular development in pigs as a model species.