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Application of Hypergeometric Series in the Inverse Moments of Special Discrete Distribution

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Journal of Applied Mathematics and Physics 2017年第2期5卷 267-275页

作者： hongyu bao Wuyun gaowa Department of Mathematics College of Sciences and Technology Inner Mongolia University Huhhot China

In this paper, we use the generalized hypergeometric series method the high-order inverse moments and high-order inverse factorial moments of the generalized geometric distribution, the Katz distribution, the Lagrangi... 详细信息

关键词： Hypergeometric Series Inverse Moments Factorial Inverse Moments

Structural and functional studies of the RhoGAP domain of ARAP3

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Structural and functional studies of the RhoGAP domain of AR...

中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者： hongyu bao School of Life Science University of Science and Technology of China

ARAP3 is a dual GAP protein containing five PH domains that is regulated by phosphatidylinositol(3,4,5)-trisphosphate[PtdIns(3,4,5)P3],a product of the phosphoinositide3-kinase(PI3K)signaling ***3 also contains a Ras association(RA)domain thatinteracts with Rap1-GTP and a sterile alpha motif(SAM)*** remaining ARAP familymembers(ARAP1/2)share ARAP3’s multidomain *** GTPases act as molecularswitches that oscillate between an GDPbound(inactive)and an GTP-bound(active)*** GDP(inactive)–GTP(active)cycle is regulated by three families of proteins:the GEFs(guanine nucleotide-exchange factors),which promot the exchange of GDP forGTP to activate Rho GTPases;the GAPs(GTPase-activating proteins),which improve the intrinsicGTPase activity to the inactive the GTPase;and the GDIs(guanine nucleotide-dissociation inhibitors),which keep Rho GTPases in the GDP-bound state and regulatetheir intracellular *** Rho family of small GTPases is involved in many cellularprocesses,including regulation of the actin cytoskeleton,cell polarity,microtubuledynamics,membrane transport pathways and transcription factor *** theRhoGAP domain ARAP3 could regulate cellular RhoA-GTP level and then control many cellularprocesses such as:Suppression of peritoneal dissemination of scirrhous gastric carcinomacells;regulation of cell adhesion and migration;regulation of chemotaxis and adhesion-dependent processes in neutrophils;control of neurite outgrowth and developmentof ***,Rap1-GTP could active the RhoGAP activity of ARAP3by directly binding to the RA domain,however the mechanism remains *** havealready resolved the 2.5 angstrom crystal structure of the RhoGAP domain of *** of the RhoGAP domain and the activation mechanism by the Rap1-GTP are understudying.

关键词： ARAP3 RhoA RhoGAP

NMR fragment screening hit induces plasticity of BRD7/9 bromodomains

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NMR fragment screening hit induces plasticity of BRD7/9 brom...

第五届中国结构生物学学术讨论会

作者： Na Wang Fudong Li hongyu bao Jie Li Jihui Wu Ke Ruan Hefei National Laboratory for Physical Science at the Microscale School of Life SciencesUniversity of Science and Technology of China

The profound biology associated with BET bromodomain inhibition using chemical probes has attracted increasing attention for identification of new non-BET bromodomain inhibitors1. Several potent inhibitors to BRD9 bromodomain have been discovered and exhibited anti-cancer and antiinflammation activities very recently2,3, however, it remains unexploited regarding its paralog BRD7 bromodomain. Here, we identify new chemotypes targeting BRD7 bromodomain using NMR fragment based screening. BRD7 and BRD9 bromodomains exhibit similar patterns of chemical shift perturbations upon the titration of hit 1. The crystal structure demonstrates that hit 1 repels Y222 group of BRD9 bromodomain in a way similar to butyryllysine（Kbu） and crotonyllysine（Kcr）, but not the acetyl lysine（Kac） and known inhibitors. Rearrangement of residue F161 is observed for hit 1, KAc, Kbu and Kcr successively, which provides structural insight into the new generation of chemical probes to BRD7 bromodomain.

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A novel RNA-binding mode of the YTH domain reveals the mechanism for recognition of determinant of selective removal by Mmi1

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A novel RNA-binding mode of the YTH domain reveals the mecha...

第九届全国核糖核酸学术讨论会

作者：王崇元 Yuwei Zhu hongyu bao Yiyang Jiang Chao Xu Jihui Wu Yunyu Shi 中国科学技术大学

＜正＞The YTH domain-containing protein Mmi1,together with other factors,constitutes the machinery used to selectively remove meiosis-specific m RNA during the vegetative growth of fission ***1 directs meiotic m RNAs to the nuclear exosome for degradation by recognizing their DSR（determinant of selective removal）***,

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A novel RNA-binding mode of the YTH domain reveals the mechanism for recognition of determinant of selective removal by Mmi1

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A novel RNA-binding mode of the YTH domain reveals the mecha...

第五届中国结构生物学学术讨论会

作者： Chongyuan Wang Yuwei Zhu hongyu bao Yiyang Jiang Chao Xu Jihui Wu Yunyu Shi Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China

The YTH domain-containing protein Mmi1, together with other factors, constitutes the machinery used to selectively remove meiosis-specific m RNA during the vegetative growth of fission yeast. Mmi1 directs meiotic m RNAs to the nuclear exosome for degradation by recognizing their DSR（determinant of selective removal） motif. Here, we present the crystal structure of the Mmi1 YTH domain in the apo state and in complex with a DSR motif, demonstrating that the Mmi1 YTH domain selectively recognizes the DSR motif. Intriguingly, Mmi1 also contains a potential m6A（N6-methyladenine）-binding pocket, but its binding of the DSR motif is dependent on a long groove opposite the m6A pocket. The DSR-binding mode is distinct from the m6A RNA-binding mode utilized by other YTH domains. Furthermore, the m6A pocket cannot bind m6A RNA. Our structural and biochemical experiments uncover the mechanism of the YTH domain in binding the DSR motif and help to elucidate the function of Mmi1.

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The complex structure of ARAP3 RhoGAP-RhoA reveals the molecular mechanism for specifically recognition of RhoA by ARAP3

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The complex structure of ARAP3 RhoGAP-RhoA reveals the molec...

第五届中国结构生物学学术讨论会

作者： hongyu bao Fudong Li Chongyuan Wang Na Wang Yiyang Jiang Yajun Tang Jihui Wu Yunyu Shi Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China

ARAP3 is unique for its dual specific GAPs（GTPase activating protein） activity for Arf6 and RhoA, regulated by Ptd Ins（3,4,5）P3 and Rap1-GTP and involving in regulation of cell shape and adhesion. However, the clear interface between the Rho GAP domain and RhoA is unknown. Meanwhile, the substrate selectivity of the Rho GAP domain is also unclear. In this study, we solved the crystal structure of RhoA in complex with the Rho GAP domain of ARAP3. The complex structure presented the clear interface between the Rho GAP domain and RhoA. By analyzing the complex structure in combination with in vitro RhoA activity assay and ITC experiments, we found the crucial residues affecting the Rho GAP activity and the substrates selectivity among RhoA, Rac1 and Cdc42. The title should be in bold Arial（14 points）, with the initial letter capitalized. Authors will start on a new line and should be in regular Times New Roman（10 points）. Family name should be placed after the first name. Underline the name of the presenting author. Affiliation of authors should start on a new line in italics with address and e-mail address. Main text should be within 400 words in regular Times New Roman（12 points） and single-spaced. Insert a single blank line space between paragraphs.

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Structural Basis for the Specific Recognition of Rho A by the Dual GTPase-activating Protein ARAP3

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Structural Basis for the Specific Recognition of Rho A by th...

中国生物化学与分子生物学会2016年全国学术会议

作者： hongyu bao Fudong Li Chongyuan Wang Na Wang Yiyang Jiang Yajun Tang Jihui Wu Yunyu Shi Hefei National Laboratory for Physical Science at Microscale Collaborative Innovation Center of Chemistry for Life Sciences and School of Life Sciences University of Science and Technology of China

ARAP3(Arf-GAP with Rho-GAP domain,ANK repeat and PH domain-containing protein 3)is unique for its dual specificity GAPs(GTPase activating protein)activity for Arf6(ADP-ribosylation factor 6)and RhoA(Ras homolog gene family member A)regulated by Ptd Ins(3,4,5)P3(phosphatidylinositol(3,4,5)-trisphosphate)and a small GTPase Rap1-GTP and is involved

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“双减”背景下的中小学教师减负路径探索

成才之路 2024年第8期 53-56页

作者：包宏宇内蒙古师范大学内蒙古呼和浩特010028

“双减”政策以学校为主阵地实施多项举措,给中小学教师工作带来诸多影响,有时也使中小学教师负担问题进一步凸显。“双减”背景下,中小学教师减负既有重要性又有紧迫性。其重要性在于为中小学教师减负是落实“双减”政策的现实之需,是... 详细信息

“双减”政策以学校为主阵地实施多项举措,给中小学教师工作带来诸多影响,有时也使中小学教师负担问题进一步凸显。“双减”背景下,中小学教师减负既有重要性又有紧迫性。其重要性在于为中小学教师减负是落实“双减”政策的现实之需,是推动教师专业化发展的外在保障,是实现教育高质量发展的内在要求。其紧迫性在于“双减”政策实施过程中,中小学教师工作呈现出的工作时间过度延长、工作量明显增多及工作难度显著上升的问题亟待解决。“双减”背景下减轻中小学教师负担应做好顶层制度设计,理清教师工作边界;优化学校管理方式,加强教师工作保障;强化家庭教育作用,推进家校协同育人;注重教师自我调整,提升教师专业素养。

关键词： “双减”政策中小学教师教师负担减负重要性路径

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工字钢在某超高层剪力墙结构中的应用

施工技术 2015年第14期44卷 36-38,62页

作者：包洪余来武清杨越中机中联工程有限公司重庆400039

重庆某超高层剪力墙结构,底部大范围两层通高,且底部两侧墙肢受拉。设计时在穿层墙和受拉剪力墙中增设了工字钢。就工字钢对剪力墙的弹塑性性能的改善进行了分析,对型钢的选型和在边缘构件及墙身中的布置进行了描述,对型钢外侧箍筋的施... 详细信息

重庆某超高层剪力墙结构,底部大范围两层通高,且底部两侧墙肢受拉。设计时在穿层墙和受拉剪力墙中增设了工字钢。就工字钢对剪力墙的弹塑性性能的改善进行了分析,对型钢的选型和在边缘构件及墙身中的布置进行了描述,对型钢外侧箍筋的施工、型钢的拼接、型钢剪力墙基础的设计进行了阐述,对梁纵筋在型钢剪力墙中的锚固连接做法进行了深化设计。验证了型钢设置的有效性和可行性。

关键词：高层建筑剪力墙工字钢弹塑性性能锚固