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响应槲皮素诱导的美国白蛾P450基因及CYP6ZB2功能分析

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中国农业科学 2023年第7期56卷 1322-1332页

作者：邵红扬孟香张涛陈敏北京林业大学林学院/林木有害生物防治北京市重点实验室

【目的】明确美国白蛾（Hyphantria cunea）响应槲皮素诱导的差异表达P450基因及系统发育关系，探究美国白蛾P450基因对槲皮素诱导的剂量效应，以及P450基因在美国白蛾对槲皮素的解毒适应中的作用。【方法】基于槲皮素诱导的美国白蛾中... 详细信息

【目的】明确美国白蛾（Hyphantria cunea）响应槲皮素诱导的差异表达P450基因及系统发育关系，探究美国白蛾P450基因对槲皮素诱导的剂量效应，以及P450基因在美国白蛾对槲皮素的解毒适应中的作用。【方法】基于槲皮素诱导的美国白蛾中肠转录组数据，从中筛选显著差异表达的P450基因，应用MEGA X软件对5个P450基因及其他近缘物种的P450基因进行系统进化关系分析；通过RT-qPCR技术检测5个P450基因在不同槲皮素浓度（0.5%、1.0%、2.0%和4.0%）处理下的表达模式；克隆CYP6ZB2全长cDNA序列，利用RNA干扰（RNAi）技术探明CYP6ZB2基因沉默对美国白蛾体重及存活率的影响。【结果】从转录组的差异表达基因中筛选到5个显著响应槲皮素诱导的P450基因，经P450命名委员会分别被命名为CYP4G296、CYP4G297、CYP333A30、CYP304F33和CYP6ZB2(GenBank登录号：ON032363—ON032367)。系统发育分析表明，这5个基因被聚类到CYP2、CYP3、CYP4和线粒体CYP进化枝。RT-qPCR结果表明，P450基因响应槲皮素胁迫具有显著的剂量效应，CYP4G297、CYP304F33、CYP333A30和CYP4G296的表达均被不同浓度槲皮素抑制，CYP6ZB2的表达在0.5%—2.0%的槲皮素诱导下显著上调，而在4.0%的槲皮素浓度下被显著抑制。克隆CYP6ZB2的全长cDNA序列，CYP6ZB2具有完整的开放阅读框（ORF），编码500个氨基酸。RNAi结果表明，注射两种剂量（500和1 000 ng）ds CYP6ZB2均可显著抑制CYP6ZB2的表达，注射1 000 ng ds CYP6ZB2 48 h后干扰效率达到最高（96.9%）。CYP6ZB2被干扰的美国白蛾幼虫经0.5%槲皮素处理后体重增长量低于对照组，存活率（53.33%）分别为未处理组的61.5%和注射双蒸水组的72.7%(P<0.05)。【结论】筛选鉴定到美国白蛾响应槲皮素诱导的5个P450基因，槲皮素对5个基因的诱导表达具有明显的剂量效应，说明不同的P450基因对槲皮素的响应模式存在差异。RNAi能高效沉默CYP6ZB2,0.5%槲皮素对CYP6ZB2被干扰的美国白蛾幼虫的生长发育具有一定抑制作用，表明CYP6ZB2在美国白蛾对槲皮素的解毒适应中发挥着重要作用。

关键词：美国白蛾槲皮素 P450基因表达分析 RNA干扰基因功能

地热能全流发电用两相单螺杆膨胀机理论膨胀过程热力特性研究

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西安交通大学学报 2024年第5期58卷 190-199页

作者：聂涛周宏洋邵华王增丽中国石油大学(华东)新能源学院山东青岛266580

针对地热能发电技术存在的系统效率与热源利用率低的问题,对全流循环发电系统中替代传统汽轮机使用的单螺杆膨胀机进行了热力特性分析。基于质量守恒方程、能量守恒方程与湿蒸汽维里状态方程,建立了考虑液体闪蒸与气液平衡的单螺杆膨胀... 详细信息

针对地热能发电技术存在的系统效率与热源利用率低的问题,对全流循环发电系统中替代传统汽轮机使用的单螺杆膨胀机进行了热力特性分析。基于质量守恒方程、能量守恒方程与湿蒸汽维里状态方程,建立了考虑液体闪蒸与气液平衡的单螺杆膨胀机理论全流膨胀过程的热力学模型,探索了进气温度与干度对气液两相工质状态及膨胀机热力特性的影响机理。结果表明:当进气温度由140℃升高至170℃时,湿蒸汽工质的质量、压力和温度均得到提升,单螺杆膨胀机的输出功率也由210 kW提升至约260 kW,等熵效率在进气温度为160℃时取得77%的最佳值;进气干度的提高会减少进入工作腔湿蒸汽工质质量,进而导致膨胀结束后介质压力与温度的降低,但显著促进了膨胀机的性能参数包括输出功率和等熵效率的提高,当进气干度为0.3时,单螺杆膨胀机的输出功率超过了500 kW,等熵效率也可达76.5%。上述研究结果对应用于地热能全流发电的两相单螺杆膨胀机的热力特性分析与改善以及全流发电技术的优化有一定的参考意义。

关键词：地热能两相湿蒸气单螺杆膨胀机热力特性

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Self-attention Based Multimodule Fusion Graph Convolution Network for Traffic Flow Prediction

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国际计算机前沿大会会议论文集 2022年第1期 3-16页

作者： Lijie Li hongyang shao Junhao Chen Ye Wang College of Computer Science and Technology Harbin Engineering UniversityHarbinChina

With rapid economic development,the per capita ownership of automobiles in our country has begun to rise year by *** researchers have paid attention to using scientific methods to solve traffic flow *** flow prediction is not simply affected by the number of vehicles,but also contains various complex factors,such as time,road conditions,and people ***,the existing methods ignore the complexity of road conditions and the correlation between individual nodes,which leads to the poor *** this study,a deep learning model SAMGCN is proposed to effectively capture the correlation between individual nodes to improve the performance of traffic flow ***,the theory of spatiotemporal decoupling is used to divide each time of each node into finer ***,multimodule fusion is used to mine the potential periodic relationships in the ***,GRU is used to obtain the potential time relationship of the three *** experiments were conducted on two traffic flow datasets,PeMS04 and PeMS08 in the Caltrans Performance Measurement System to prove the validity of the proposed model.

关键词： Flow prediction Temporal-spatial correlation Graph convolution network Self-attention mechanism

高胆固醇血症患者中筛查家族性高胆固醇血症的临床意义研究

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中国全科医学 2024年第36期27卷 4515-4521页

作者：李渊马弘阳李标岳岸娜邵娅庆孙康云南京医科大学附属苏州医院心血管病中心江苏省苏州市215008 南京医科大学姑苏学院江苏省苏州市215000

背景家族性高胆固醇血症(FH)是一种常染色体(共)显性遗传病,是严重的高胆固醇血症,血清低密度脂蛋白胆固醇(LDL-C)水平明显升高,这部分患者早发动脉粥样硬化性心血管疾病风险明显增加,早发现和尽早接受治疗可改善患者的存活率。目的在... 详细信息

背景家族性高胆固醇血症(FH)是一种常染色体(共)显性遗传病,是严重的高胆固醇血症,血清低密度脂蛋白胆固醇(LDL-C)水平明显升高,这部分患者早发动脉粥样硬化性心血管疾病风险明显增加,早发现和尽早接受治疗可改善患者的存活率。目的在高胆固醇血症患者中筛查FH,探讨社区人群筛查FH的临床价值、意义。方法2023年7—12月纳入南京医科大学附属苏州医院心血管病中心及下属医联体5个社区卫生中心LDL-C≥4.90 mmol/L并完成基因测序的高胆固醇血症患者164例,根据LDL-C水平的四分位间距,分为Q1组(4.90 mmol/L≤LDL-C0.05)。结论社区人群高胆固醇血症LDL-C≥4.9 mmol/L的患者中,FH基因变异率较高,同时其他原发性(遗传性)脂代谢基因变异率较高,在社区人群高胆固醇血症患者中筛查FH有重要临床意义和价值。

关键词：高胆固醇血症家族性高胆固醇血症筛查临床意义

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Chinese Society of Cardiology guidelines on the diagnosis and treatment of adult fulminant myocarditis

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Science China(Life Sciences) 2024年第5期67卷 913-939页

作者： Chinese Society of Cardiology,Chinese Medical Association,Writing Group Jiangang Jiang hongyang Shu Dao Wen Wang Rutai Hui Chenze Li Xiao Ran Hong Wang Jing Zhang shaoping Nie Guanglin Cui Dingcheng Xiang Qun shao Shengyong Xu Discussion experts:Ning Zhou Yuming Li Wei Gao Yuguo Chen Yuan Bian Guoping Wang Liming Xia Yan Wang Chunxia Zhao Zhiren Zhang Yuhua Zhao Jianan Wang shaoliang Chen Hong Jiang Jing Chen Xianjin Du Mao Chen Yinxian Sun Sheng Li Hu Ding Xueping Ma Hesong Zeng Li Lin Shenghua Zhou Likun Ma Ling Tao Juan Chen Yiwu Zhou Xiaomei Guo 不详 Tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Fuwai Hospital National Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China Zhongnan Hospital of Wuhan University Wuhan 430062China Fuwai Huazhong Cardiovascular Hospital Zhengzhou 450003China Beijing Anzhen Hospital Capital Medical UniversityBeijing 100029China Guangzhou General Hospital of Guangzhou Military Command Guangzhou 510010China Harbin Medical University Cancer Hospital Harbin 150081China Union Hospital Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Taida Hospital Tianjin 300457China Peking University Third Hospital Beijing 100191China Qilu Hospital of Shandong University Jinan 250012China Kanghua Hospital DongguanGuangzhou 523080China Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003China Nanjing First Hospital Nanjing Medical UniversityNanjing 210006China Renmin Hospital of Wuhan University Wuhan 430060Wuhan West China Hospital Sichuan UniversityChengdu 610044China First Hospital of China Medical University Shenyang 110002China General Hospital of Ningxia Medical University Yinchuan 750003China The Second Xiangya Hospital Central South UniversityChangsha 410012China The First Affiliated Hospital of USTC University of Science and Technology of ChinaHefei 230002China The First Affiliated Hospital of Air Force Medical University Xi’an 710032China Central Hospital of Wuhan City Wuhan 430014China Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030China

Fulminant myocarditis is an acute diffuse inflammatory disease of *** is characterized by acute onset,rapid progress and high risk of *** pathogenesis involves excessive immune activation of the innate immune system and formation of inflammatory *** to China’s practical experience,the adoption of the“life support-based comprehensive treatment regimen”(with mechanical circulation support and immunomodulation therapy as the core)can significantly improve the survival rate and long-term *** emphasis is placed on very early identification,very early diagnosis,very early prediction and very early treatment.

关键词： fulminant myocarditis guidelines life support-based comprehensive treatment regimen diagnosis treatment cytokine storm overactivation of immunity

硅钼蓝光度法测定油田采出水中可溶性SiO_2含量的改进

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实验室研究与探索 2018年第12期37卷 4-8页

作者：李美蓉邵洪扬周海刚操应长中国石油大学(华东)理学院山东青岛266580 中国石化胜利油田公司技术检测中心山东东营257000 中国石油大学(华东)地球科学与技术学院山东青岛266580

为建立一种更为准确的油田采出水硅含量的测定方法,对标准GB/T12149—2007《工业循环冷却水和锅炉用水中硅的测定》中SiO_2的测定方法进行了改进。通过对硅钼蓝分光光度法的多个实验条件的优化,得到优选的测定条件为:室温下取0. 5mL待... 详细信息

为建立一种更为准确的油田采出水硅含量的测定方法,对标准GB/T12149—2007《工业循环冷却水和锅炉用水中硅的测定》中SiO_2的测定方法进行了改进。通过对硅钼蓝分光光度法的多个实验条件的优化,得到优选的测定条件为:室温下取0. 5mL待测样品,加入1+1盐酸1mL,100 g/L钼酸铵溶液1. 5mL,静置10 min后加入1+1硫酸溶液1. 0mL和20 g/L抗坏血酸溶液1. 5mL,定容于50mL比色管,摇匀静置25 min,在波长810 nm下测定。SiO_2浓度在0. 01～1. 2mg/L呈现良好的线性关系,线性方程:A=0. 761 59c,R=0. 999 94。相对标准偏差RSD <1. 0%,加标回收率为99. 4%～102. 5%。结果表明,该方法重现性和准确度较好,可准确分析油田采出水中的可溶性SiO_2。

关键词：油田采出水可溶性 SiO2含量硅钼蓝分光光度法

施用有机肥和调理剂对田间土壤重金属有效态变化影响及水稻降镉效应研究

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中国稻米 2024年第2期30卷 37-42页

作者：黄奇娜党洪阳张燕李春福林光号邵国胜季卫东中国水稻研究所杭州310006 常山县农业农村局浙江常山324200

以春优84和中浙优8号为试验材料,在浙江衢州典型镉污染农田开展大田小区试验,研究有机肥与土壤调理剂施用对土壤酸碱度(pH)、土壤重金属元素(Cd、Fe、Mn、Zn等)有效态、水稻植株(稻草、籽粒)重金属元素积累量的影响,并探究了施用有机肥... 详细信息

以春优84和中浙优8号为试验材料,在浙江衢州典型镉污染农田开展大田小区试验,研究有机肥与土壤调理剂施用对土壤酸碱度(pH)、土壤重金属元素(Cd、Fe、Mn、Zn等)有效态、水稻植株(稻草、籽粒)重金属元素积累量的影响,并探究了施用有机肥与土壤调理剂降低籽粒Cd积累的机理。结果表明,单施有机肥(1.5 t/hm^(2))对土壤金属元素有效态、稻草和籽粒重金属元素含量无显著影响;在有机肥(1.5 t/hm^(2))+土壤调理剂(2.7 t/hm^(2))联合施用处理后,土壤pH增加9.48%,土壤重金属有效态含量和植株中重金属元素含量均呈下降趋势。其中,土壤有效态Cd含量降低16.41%(DTPA提取)和48.96%(CaCl_(2)提取),稻草和籽粒Cd含量分别降低40.04%和48.22%。逐步回归和通径分析表明,稻草Cd含量、土壤Cd有效态与籽粒Cd含量显著相关。由此可见,施用土壤调理剂通过提升土壤pH,从而降低土壤重金属元素有效态含量,其中有效态Cd含量下降尤为显著,以此调控水稻稻草和籽粒中的Cd积累。说明供试土壤调理剂可以在酸性土地区推广应用,具有提升酸性土壤pH、降低稻米Cd富集的效果。

关键词：水稻有机肥土壤调理剂重金属镉有效态

Chinese Society of Cardiology guidelines on the diagnosis and treatment of adult fulminant myocarditis

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Science China Life Sciences 2024年

作者： Jiangang Jiang hongyang Shu Dao Wen Wang Rutai Hui Chenze Li Xiao Ran Hong Wang Jing Zhang shaoping Nie Guanglin Cui Dingcheng Xiang Qun shao Shengyong Xu Ning Zhou Yuming Li Wei Gao Yuguo Chen Yuan Bian Guoping Wang Liming Xia Yan Wang Chunxia Zhao Zhiren Zhang Yuhua Zhao Jianan Wang shaoliang Chen Hong Jiang Jing Chen Xianjin Du Mao Chen Yinxian Sun Sheng Li Hu Ding Xueping Ma Hesong Zeng Li Lin Shenghua Zhou Likun Ma Ling Tao Juan Chen Yiwu Zhou Xiaomei Guo Peking University Third Hospital Qilu Hospital of Shandong University The First Affiliated Hospital of Air Force Medical University Zhongnan Hospital of Wuhan University First Hospital of China Medical University General Hospital of Ningxia Medical University The First Affiliated Hospital of USTC University of Science and Technology of China Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing Anzhen Hospital Capital Medical University Chinese Medical Association Harbin Medical University Cancer Hospital Union Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Renmin Hospital of Wuhan University Central Hospital of Wuhan City Taida Hospital Guangzhou General Hospital of Guangzhou Military Command West China Hospital Sichuan University Nanjing First Hospital Nanjing Medical University Chinese Society of Cardiology Kanghua Hospital Dongguan Fuwai Huazhong Cardiovascular Hospital Tongji Hospital Tongji Medical College Huazhong University of Science and Technology The Second Xiangya Hospital Central South University Second Affiliated Hospital Zhejiang University School of Medicine

Fulminant myocarditis is an acute diffuse inflammatory disease of myocardium. It is characterized by acute onset, rapid progress and high risk of death. Its pathogenesis involves excessive immune activation of the innate immune system and formation of inflammatory *** to China’s practical experience, the adoption of the “life support-based comprehensive treatment regimen”(with mechanical circulation support and immunomodulation therapy as the core) can significantly improve the survival rate and long-term prognosis. Special emphasis is placed on very early identification,very early diagnosis,very early prediction and very early treatment.

关键词：

Association between soft drink consumption types and risk of lung cancer and all-cancer: A prospective study of PLCO data

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The Journal of Biomedical Research 2022年第6期36卷 390-400页

作者： Dongfang You hongyang Xu Xin Chen Jiawei Zhou Yaqian Wu Yingdan Tang Zhongtian Wang Yang Zhao Fang shao Department of Biostatistics School of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Department of Critical Care Medicine Wuxi People's Hospital Affiliated to Nanjing Medical UniversityWuxiJiangsu 214023China China International Cooperation Center for Environment and Human Health Center for Global HealthNanjing Medical UniversityNanjingJiangsu 211166China The Center of Biomedical Big Data and the Laboratory of Biomedical Big Data Nanjing Medical UniversityNanjingJiangsu 211166China Jiangsu Key Lab of Cancer Biomarkers Prevention and TreatmentCollaborative Innovation Center for Cancer Personalized MedicineNanjing Medical UniversityNanjingJiangsu 211166China

Diet/sugar-free soft drinks are considered to be healthier than regular soft ***,few studies have examined the relationship between the types of soft drinks(regular and diet/sugar-free)and lung cancer(LC)/all-cancer(AC)*** this study,we comprehensively assessed the influence of the type of soft drink consumption on LC/AC risk based on the Prostate,Lung,Colorectal,and Ovarian(PLCO)Cancer Screening *** Cox proportional hazards and competing risks Fine-Gray regression models adjusted for relevant confounders were used to estimate hazard ratios(HRs)and subdistribution HRs for different types of soft drink *** the PLCO population,female subgroup,and the ever/current smoker subgroup,consumption of both regular and diet soft drinks was associated with a significantly reduced risk of LC compared with no soft drinks at *** the non-lung cancer(NLC)risk,consumption of only diet soft drinks had a significant positive association for the total population and female *** on our findings,it was suggested that partial replacement of regular soft drinks with diet soft drinks might be beneficial to LC prevention,especially for females and ever/current ***,completely replacing regular soft drinks with diet soft drinks might be detrimental to NLC prevention,especially for females.

关键词： soft drink diet/sugar-free drink lung cancer all-cancer PLCO trial