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Origin and evolution of a new tetraploid mangrove species in an intertidal zone

Plant Diversity 2024年第4期46卷 476-490页

作者： Hui Feng Achyut Kumar Banerjee Wuxia Guo Yang Yuan Fuyuan duan Wei Lun ng Xuming Zhao Yuting Liu Chunmei Li Ying Liu Linfeng Li Yelin Huang State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources School of Life SciencesSun Yat-sen UniversityGuangzhou 510275GuangdongChina Department of Bioengineering Zhuhai Campus of Zunyi Medical UniversityZhuhai 519041GuangdongChina China-ASEAN College of Marine Sciences Xiamen University MalaysiaSepang 43900SelangorMalaysia School of Agriculture Sun Yat-sen UniversityShenzhen 518107GuangdongChina School of Ecology Sun Yat-sen UniversityShenzhen 518107GuangdongChina

Polyploidy is a major factor in the evolution of plants,yet we know little about the origin and evolution of polyploidy in intertidal *** study aimed to identify the evolutionary transitions in three truemangrove species of the genus Acanthus distributed in the Indo-West Pacific *** this purpose,we took an integrative approach that combined data on morphology,cytology,climatic niche,phylogeny,and biogeography of 493 samples from 42 geographic *** results show that the Acanthus ilicifolius lineage distributed east of the Thai-Malay Peninsula possesses a tetraploid karyotype,which is morphologically distinct from that of the lineage on the west *** haplotype networks and phylogenetic trees for the chloroplast genome and eight nuclear genes reveal that the tetraploid species has two sub-genomes,one each from *** and ***,the paternal and maternal parents,*** structure analysis also supports the hybrid speciation history of the new tetraploid *** two sub-genomes of the tetraploid species diverged from their diploid progenitors during the *** niche models revealed that the tetraploid species not only occupied the near-entire niche space of the diploids,but also expanded into novel *** findings suggest that *** species distributed on the east side of the Thai-Malay Peninsula should be regarded as a new species,***,which originated from hybridization between *** and ***,followed by chromosome *** is the first report of a true-mangrove allopolyploid species that can reproduce sexually and clonally reproduction,which explains the long-term adaptive potential of the species.

关键词： Acanthus Allopolyploid Biogeography Evolution Hybridization Polyploidy

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Crystalline-amorphous M@MN_x(M=Co,Fe,Ni)encapsulated in nitrogen-doped carbon for enhanced efficient and durable hydrogen evolution reaction

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Rare Metals 2024年第4期43卷 1547-1556页

作者： Yang Yang Xiao-Chuan duan Shao-Hui Guo Xian-Ming Zhang College of Materials Science and Engineering College of ChemistryKey Laboratory of Interface Science and Engineering in Advanced MaterialsTaiyuan University of TechnologyYuci030600China

Developing earth-abundant electrocatalysts for hydrogen evolution reaction(HER)is important for the sustainable energy ***,efficient and stable heterocatalysts consisting of crystalline-amorphous M@MN_(x)(M=Co,Fe,Ni)encapsulated in N-doped carbon layers supported with N-doped graphene sheets(denoted as M@MN_(x)@NC-ng)are synthesized by facile hydrothermal reaction and nitridation *** the nitriding process,metal ions in M(tzbc)_2(H_(2)O)_4(tzbc=4-(1H-1,2,4-triazol-1-yl)benzoic acid)complexes are reduced to crystalline M cores,accompanied by the formation of amorphous MN_(x)shells;the tzbc ligands are insitu carbonized to form outermost N-doped carbon(NC)layers that connect with inner MN_(x)via M-N-C motifs inherited from the complex precursors and inhibit the transition of MN_(x)from amorphous to crystalline *** Co@CoN_(x)@NC-ng catalyst exhibits excellent HER activity with small overpotentials of 45 and 64 mV at a cathode current density of 10 mA·cm^(-2)and low Tafel slopes of 40 and 85 mV·dec^(-1)in 0.5 mol·L^(-1)H_(2)SO_4 and1.0 mol·L^(-1)KOH electrolytes,*** Co@CoN_(x)@NC-ng retains 97%of the initial overpotential after 100,000 s in both acidic and alkaline *** outstanding HER performance originates from the crystalline-amorphous Co@CoN_(x)that redistributes electrons around the heterointerfaces,facilitating the conversion process of H^(+)/H_(2)O to hydrogen and thereby promoting HER *** outermost NC layers serve as the armor of Co@CoN_(x),and graphene nanosheets act as carriers of egg-like Co@CoN_(x)@NC and conduction paths for electron shuttles,ensuring stable and continuous electrocatalytic hydrogen production.

关键词： Hydrogen evolution reaction Electrocatalysts Iron group metals Heterojunction Crystalline-amorphous structure

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Highly sensitive nanozyme strip:An effective tool for forensic material evidence identification

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Nano Research 2024年第3期17卷 1785-1791页

作者： Juanji Hong Zhanjun Guo Dihan duan Yi Zhang Xin Chen Yongjiu Li Zheng Tu Lei Feng Lei Chen Xiyun Yan Lizeng Gao Minmin Liang Demin duan Experimental Center of Advanced Materials School of Materials Science&EngineeringBeijing Institute of TechnologyBeijing 100081China College of Bioscience and Biotechnology Shenyang Agricultural UniversityShenyang 110866China Chongqing kangjuquanhong biology science and technology Co. ltdChongqing 400026China Key Laboratory of Forensic Genetics Institute of Forensic ScienceMinistry of Public SecurityBeijing 100038China CAS Engineering Laboratory for Nanozyme Key Laboratory of BiomacromoleculesInstitute of BiophysicsChinese Academic of ScienceBeijing 100101China

During criminal case investigations,blood evidence tracing is critical for criminal ***,the blood stains are often cleaned or covered up after the crime,resulting in trace residue and difficult ***,a highly sensitive and specific method for the rapid detection of human blood stains remains *** solve this problem,we established a nanozyme-based strip for rapid detection of blood evidence with high sensitivity and *** construct reliable nanozyme strips,we synthesized CoFe_(2)O_(4) nanozymes with high peroxidase-like activity by scaling up to gram level,which can be supplied for six million tests,and conjugated antibody as a detection probe in nanozyme *** developed CoFe_(2)O_(4) nanozyme strip can detect human hemoglobin(HGB)at a concentration as low as 1 ng/mL,which is 100 times lower than the commercially available colloidal gold strips(100 ng/mL).Moreover,this CoFe_(2)O_(4) nanozyme strip showed high generality on 12 substrates and high specificity to human HGB among 13 animal blood ***,we applied the developed CoFe_(2)O_(4) nanozyme strip to successfully detect blood stains in three real cases,where the current commercial colloidal gold strip failed to *** results suggest that the CoFe_(2)O_(4) nanozyme strip can be used as an effective on-scene detection method for human blood stains,and can further be used as a long-term preserved material evidence for traceability inquiry.

关键词： CoFe_(2)O_(4)nanozyme nanozyme strip peroxidase-like activity human hemoglobin blood evidence

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Selenium Regulates Antioxidant Capacities and Diterpenoid Biosynthesis in the Medicinal Plant Isodon rubescens

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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1705-1716页

作者： Fenglan Zhao Shuwen Wu Xue Meng Jianping Xue Yongbo duan Anhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants College of Life SciencesHuaibei Normal UniversityHuaibei235000China Huaibei Key Laboratory of Efficient Cultivation and Utilization of Resource Plants College of Life SciencesHuaibei Normal UniversityHuaibei235000China

Dōng líng căo,the dried aboveground parts of Isodon rubescens(Hemls.)Hara.,is commonly consumed as a med-icinal decoction or tea *** beverages can be an important source of human dietary selenium(Se).However,how *** plants respond to exogenous Se remains *** this study,a pot cultivation experiment was employed to investigate the phenotypic and physiological responses of *** plants exposed to *** days after applying different concentrations of sodium selenate to the soil,the Se enrichment capa-city,growth indices,antioxidant capacities,and the content ofﬂavonoids and diterpenoids were measured in the ***,the oridonin content was quantiﬁed using the high-performance liquid chromatography method,and the expression levels of key diterpenoid synthesis genes were analyzed by quantitative real-time PCR(qRT-PCR).*** plants efﬁciently accumulated Se,with the Se content increasing proportionally to the applied dose,reaching levels of nearly 200 mg·kg^(-1) dry leaves as Se concentration *** of the three Se treat-ments signiﬁcantly altered the phenotypic indices,except a longer root length occurred in the 3μM·kg^(-1) Se *** three Se doses,6μM·kg^(-1) Se gave the highest accumulation ofﬂavonoids,diterpenoids,and oridonin,with the increase of 2.0-,1.8-,and 1.9-fold in aboveground parts,*** application boosted the activities of antioxidant enzymes and antioxidant capacities according to 2,2-Diphenyl-1-picrylhydrazyl(DPPH),ferric reducing/antioxidant power,and tea brewing color *** key synthase genes were upregulated signiﬁcantly by 6μM·kg^(-1) Se treatment,notably 1-deoxy-D-xylulose 5-phosphate reductoisomerase(IrDXR),with a 5-fold increase,and kaurene synthase-like 4(IrKSL4),with a 6-fold ***,Se application in *** cultivation may be a potential biofortiﬁcation method to supplement Se while increasingﬂavonoid and diterpenoid contents.

关键词： Flavonoids oridonin physiological indicators quantitative real-time PCR selenium

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云南省多民族初一年级学生饮食模式潜类别与抑郁症状的关联

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中国学校卫生 2024年第2期45卷 168-172,177页

作者：徐洪吕王继学杨继宗杨婉娟张明付允段亚娟常利涛昆明学院医学院护理系云南650214 昆明学院社区护理研究团队双江自治县第一完全中学南涧县第二中学南涧县疾病预防控制中心免疫规划科镇沅县民族中学云南省疾病预防控制中心学校卫生所

目的探讨云南省多民族初一年级学生饮食模式潜类别及其与抑郁症状的关联,为边疆中学生心理健康促进提供参考依据。方法2022年10—12月,采用整群随机抽样方法抽取云南省11个县8500名初一年级学生进行问卷调查。使用儿童抑郁量表(CDI)评... 详细信息

目的探讨云南省多民族初一年级学生饮食模式潜类别及其与抑郁症状的关联,为边疆中学生心理健康促进提供参考依据。方法2022年10—12月,采用整群随机抽样方法抽取云南省11个县8500名初一年级学生进行问卷调查。使用儿童抑郁量表(CDI)评估学生抑郁症状,应用饮食频率调查表收集学生饮食行为数据。使用潜在剖面分析模型拟合学生饮食模式潜类别,应用Logistic回归分析饮食模式潜类别与抑郁症状的关联。结果初一年级学生抑郁症状检出率为28.3%,女生抑郁症状检出率(30.9%)高于男生(25.5%),差异有统计学意义(χ^(2)=29.83,P<0.01)。初一年级学生饮食模式有4种潜类别:类别1(所有饮食低消费),类别2(水果、奶及奶制品高消费),类别3(蔬菜、畜肉高消费,加工食品低消费),类别4(奶及奶制品和蛋类消费低,加工食品消费高)。以饮食模式类别1为参照,在控制混杂变量后,饮食模式类别3与初一年级学生抑郁症状呈负相关(OR=0.62,95%CI=0.52~0.74);类别4与初一年级学生抑郁症状呈正相关(OR=1.28,95%CI=1.05~1.57)(P值均<0.05)。结论云南省多民族初一年级学生饮食模式多样,不健康饮食模式可能增加抑郁症状发生的风险。应调整云南省多民族中学生饮食模式,促进其健康饮食模式的建立,降低中学生抑郁症状发生的风险。

关键词：饮食习惯抑郁精神卫生回归分析学生少数民族

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Highly sensitive detection of mercury（Ⅱ） ions with few-layer molybdenum disulfide

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Nano Research 2015年第1期8卷 257-262页

作者： Shan Jiang Rui Cheng Rita ng Yu Huang Xiangfeng duan Department of Chemistry and Biochemistry University of California Los Angeles California 90095 USA Department of Materials Science and Engineering University of California Los Angeles California 90095 USA California Nanosystems Institute University of California Los Angeles California 90095 USA

Two-dimensional （2D） layered transition metal dichalcogenide （TMD） materials （e.g., MoS2） have attracted considerable interest due to their atomically thin geometry and semiconducting electronic properties. With ultrahigh surface to volume ratio, the electronic properties of these atomically thin semiconductors can be readily modulated by their environment. Here we report an investigation of the effects of mercury（II）（Hg^2＋） ions on the electrical transport properties of few-layer molybdenum disulfide （MoS2）. The interaction between Hg^2＋ ions and few-layer MoS2 was studied by field-effect transistor measurements and photoluminescence. Due to a high binding affinity between Hg2. ions and the sulfur sites on the surface of MoS2 layers, Hg^2＋ ions can strongly bind to MoS2. We show that the binding of Hg^2＋ can produce a p-type doping effect to reduce the electron concentration in n-type few-layer MoS2. It can thus effectively modulate the electron transport and photoluminescence properties in few-layer MoS2. By monitoring the conductance change of few-layer MoS2 in varying concentration Hg2~ solutions, we further show that few-layer MoS2 transistors can function as highly sensitive sensors for rapid electrical detection of Hg^2＋ ion with a detection limit of 30 pM.

关键词： molybdenum disulfide 2D layered materials mercury doping effect sensors

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格列齐特缓释片在中国健康受试者中的生物等效性研究

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中国临床药理学杂志 2024年第15期40卷 2241-2245页

作者：段舟萍赵小伟温金华黄世博李蒲曹端文南昌大学第一附属医院临床试验研究中心江西南昌330006 浙江京新药业股份有限公司浙江绍兴312500

目的评价格列齐特缓释片中国健康受试者体内的生物等效性。方法采用单中心、开放、随机、单剂量、两周期、两序列给药的试验方法设计。受试者在空腹或餐后状态下口服受试制剂/参比制剂30 mg,自身交叉给药,清洗期为10 d。用液相色谱-串... 详细信息

目的评价格列齐特缓释片中国健康受试者体内的生物等效性。方法采用单中心、开放、随机、单剂量、两周期、两序列给药的试验方法设计。受试者在空腹或餐后状态下口服受试制剂/参比制剂30 mg,自身交叉给药,清洗期为10 d。用液相色谱-串联质谱(LC-MS/MS)法测定人血浆样品中格列齐特的血药浓度;用WinNonlin软件的非房室模型对格列齐特缓释片主要药代动力学参数C_(max)、AUC_(0-t)、AUC_(0-∞)进行分析。结果空腹组入组24例受试者,22例完成试验。空腹组中格列齐特缓释片受试制剂和参比制剂的主要药代动力学参数如下:C_(max)分别为(862.48±294.48)和(902.96±259.09)ng·mL^(-1),AUC_(0-t)分别为(2.60×10^(4)±8930.46)和(2.50×10^(4)±7573.42)h·ng·mL^(-1),AUC_(0-∞)分别为(3.00×10^(4)±1.43×10^(4))和(2.68×10^(4)±7085.99)h·ng·mL^(-1)。餐后组入组24例受试者,23例完成试验。餐后组中格列齐特缓释片受试制剂和参比制剂的主要药代动力学参数如下:C_(max)分别为(1531.74±273.49)和(1510.87±241.08)ng·mL^(-1),AUC_(0-t)分别为(2.78×10^(4)±9565.89)和(2.76×10^(4)±9821.43)h·ng·mL^(-1),AUC_(0-∞)分别为(3.02×10^(4)±1.24×10^(4))和(3.0×10^(4)±1.30×10^(4))h·ng·mL^(-1)。受试制剂与参比制剂格列齐特缓释片的C_(max)、AUC_(0-t)、AUC_(0-∞)的几何均值比值的90%的置信区间均落在80%~125%。结论格列齐特缓释片受试制剂和参比制剂具有生物等效性。

关键词：格列齐特缓释片健康受试者生物等效性药代动力学液相色谱-串联质谱法

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阿帕替尼血药浓度测定方法的建立及临床应用

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中国药房 2024年第12期35卷 1500-1504页

作者：段贤春薛苏君祝永福安徽中医药大学第一附属医院药学部合肥230031 安徽中医药大学新安医学教育部重点实验室合肥230012 安徽中医药大学第一附属医院肿瘤一科合肥230031

目的建立测定阿帕替尼血药浓度的方法并进行临床应用。方法采用超高效液相色谱(UPLC)法进行血药浓度测定,色谱柱为ACQUITY UPLC BEH C18,流动相为乙腈-0.1%甲酸水溶液(梯度洗脱),流速为0.2 mL/min,柱温为40℃,进样量为5μL。收集26例... 详细信息

目的建立测定阿帕替尼血药浓度的方法并进行临床应用。方法采用超高效液相色谱(UPLC)法进行血药浓度测定,色谱柱为ACQUITY UPLC BEH C18,流动相为乙腈-0.1%甲酸水溶液(梯度洗脱),流速为0.2 mL/min,柱温为40℃,进样量为5μL。收集26例服用阿帕替尼癌症患者的病例资料,检测其血药浓度,分析阿帕替尼血药浓度与患者年龄、给药剂量、不良反应及联合用药的相关性,并检测患者治疗前后血清肾损伤相关因子[胱抑素C(CysC)、肾损伤分子1(KIM-1)、白细胞介素18(IL-18)、肿瘤坏死因子α(TNF-α)]水平。结果阿帕替尼检测质量浓度在500~2 000 ng/mL范围内线性关系良好,精密度试验RSD为3.7%,稳定性试验RSD为4.9%,平均加样回收率为96.0%(RSD为2.1%)。26例患者的阿帕替尼血药浓度最低为103 ng/mL,最高为1 932ng/mL。患者的血药浓度随年龄呈波动降低趋势。在0.125或0.25 g给药剂量下,服用阿帕替尼的患者体内血药浓度集中在1 000~2 000 ng/mL范围内。26例癌症患者中有13例发生不良反应,其中血药浓度500~ng/mL者未见不良反应发生。20例患者同时联用了其他药物,其血药浓度高低不同。治疗后,患者的血清CysC、KIM-1、IL-18、TNF-α水平均显著高于治疗前(P<0.05)。结论所建立的UPLC方法能快速检测阿帕替尼血药浓度。临床使用阿帕替尼时应综合考虑患者年龄和药物联用等情况,并注意防范阿帕替尼可能导致的急性肾损伤。

关键词：阿帕替尼血药浓度监测超高效液相色谱肾损伤不良反应

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Vaspin通过调控脂肪组织巨噬细胞极化改善2型糖尿病大鼠的胰岛素敏感性

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中华内分泌代谢杂志 2024年第2期40卷 145-157页

作者：庞少博刘师伟段瑞雪赵宇翔山西医科大学第三医院(山西白求恩医院山西医学科学院同济山西医院)内分泌科太原030032 山西白求恩医院(山西医学科学院同济山西医院)、山西医科大学第三医院内分泌科太原030032

目的探讨脂肪组织丝氨酸蛋白酶抑制剂(visceral adipose tissue-derived serpin,Vaspin)对脂肪组织巨噬细胞极化的影响,为Vaspin改善糖代谢提供新的理论依据。方法选取8周龄SD雄性大鼠50只,随机分为正常组(NC组)、2型糖尿病组(T2DM组)... 详细信息

目的探讨脂肪组织丝氨酸蛋白酶抑制剂(visceral adipose tissue-derived serpin,Vaspin)对脂肪组织巨噬细胞极化的影响,为Vaspin改善糖代谢提供新的理论依据。方法选取8周龄SD雄性大鼠50只,随机分为正常组(NC组)、2型糖尿病组(T2DM组)、不同浓度Vaspin干预组(V1:480 ng/kg、V2:960 ng/kg、V3:1440 ng/kg)。Vaspin腹腔注射干预大鼠8周,干预结束后采集外周血分析糖脂代谢相关指标,通过腹腔注射葡萄糖耐量试验(intraperitoneal glucose tolerance test,IPGTT)、腹腔注射胰岛素耐量试验(intraperitoneal insulin tolerance test,IPITT)、高胰岛素-正葡萄糖钳夹技术评估各组大鼠糖耐量和胰岛素抵抗。通过免疫荧光技术(immunofluorescence,IF)测定附睾白色脂肪组织(epididymal white adipose tissue,eWAT)中CD11c、CD206、PPARγ荧光表达;实时荧光定量PCR(real-time polymerase chain reaction,RT-PCR)测定eWAT CD11c和CD206及肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β及IL-10的mRNA表达水平;酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血清中TNF-α、IL-1β及IL-10的浓度;免疫印迹法(Western blotting,WB)检测eWAT中iNOS、Arg-1、p-Akt、Akt和PPARγ的蛋白表达。结果干预8周后,各组的体重及血脂水平差异无统计学意义,IPGTT、IPITT及高胰岛素-正葡萄糖钳夹实验显示Vapsin干预组血糖及胰岛素敏感性改善,且呈剂量依赖性(P<0.05);IF及RT-PCR显示Vaspin可下调eWAT中CD11c、IL-1β、TNF-α的表达,上调CD206、IL-10、PPARγ的表达,且与Vaspin干预浓度相关(P<0.05);ELISA显示Vaspin干预可下调血清中IL-1β、TNF-α的浓度,上调IL-10的浓度(P<0.05);WB显示Vaspin可下调iNOS蛋白的表达,上调Arg1、p-Akt及PPARγ的表达,且呈剂量依赖性(P<0.05)。结论Vaspin通过上调巨噬细胞PPARγ通路,使脂肪组织巨噬细胞向M2型极化,改善脂肪组织炎症因子谱,从受体后水平改善胰岛素抵抗,从而改善T2DM大鼠的糖耐量。

关键词：糖尿病 2型脂肪组织丝氨酸蛋白酶抑制剂胰岛素抵抗脂肪组织炎症巨噬细胞极化

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Characteristics and source identification of fine particulate n-alkanes in Beijing, China

引用

Journal of Environmental Sciences 2010年第7期22卷 998-1005页

作者： Fengkui duan Kebin He Xiande Liu State Key Joint Laboratory of Environment Simulation and Pollution Control Department of Environmental Science & Engineering Tsinghua University Beijing 100084 China Chinese Research Academy of Environmental Sciences Beijing 100012 China

Ambient particulate n-alkanes were determined for fine particle （PM2.5） samples collected from Sep 2003 to July 2004 in Beijing, China. The average concentration of total n-alkanes （∑n-alkanes） from Cll to C34 was 425.72 ng/m^3, ranged from 7.02 to 2893.28 ng/m^3. The concentration distributions of n-alkanes homologues in this study exhibited peaks at C21 and C29 in heating season, and C29 in non-heating season. The average carbon preference index （CPI） value was 1.88 in the range of 1.18-3.88. The maximum CPI in summer indicated the contribution of biogenic origins such as plant wax; while the minimum CPI value in winter was probably a result of fossil fuel combustion. Preliminary estimation from these results showed that 59% of the n-alkanes in PM2.5 in Beijing summer originated from plant wax, while 74%-88% was from fossil fuel combustion in other three seasons. Source estimation was further performed using principal component analysis method. Two major components were yielded accounting for 57.3% and 30.9% of the total variance, which presented the fossil fuel and biogenic contribution, respectively.

关键词： n-alkanes PM2.5 carbon preference index principal component analysis

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