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2015中国遗传学会大会

作者： hongju jian Jia Wang Tengyue Wang Lijuan Wei jiana Li Wei Qian Liezhao Liu College of Agronomy and Biotechnology Southwest University

Drought and salinity stresses are two of the more severe and wide-ranging abiotic stresses that significantly affect crop germination,development and *** animals,plants are stationary organisms that have evolved mechanisms to cope with a wide range of environmental and climate changes by regulating genes expression at several *** we know,*** is the third most economically important *** all crops,exposure of soil salinity and drought stresses at specific developmental stages of Brassicas leads to compromised growth,development and *** the seed germination is the first critical step in growth and development of plant whole *** investigate small RNA targets comprehensively and provide basic information for further understanding the miRNA-mediated post-transcriptional regulation network during oilseed seed germination process under drought and salt stress,three small RNA libraries were constructed using RNA from tissues during germination under salt(S),drought(D)and distilled water(CK)from *** genotype ZS11,and sequenced using an Illumina Genome Analyzer.A total of 11,528,557,12,080,081and 12,315,608 raw reads were obtained from CK,D and S libraries,*** analysis confirmed 85,81 and 81 known miRNAs families belonging to 31,30 and 30 miRNA families and the discovery of 418,430 and415 novel miRNAs in CK,D and S libraries,*** targets for 336,376 and 340 novel miRNAs were successfully predicted in CK,D and S libraries,*** sequencing results were partially validated by quantitative RT-PCR for both conserved and novel miRNAs and the predicted target *** data suggested that diverse and complex miRNAs are involved in the seed imbibition *** miRNA are involved in plant hormone regulation may play important roles during the seed germination under salt-or drought stress conditions.

关键词： Drought stress Salt stress MicroRNA Brassica napus Seed germination\

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Integrated mRNA,sRNA,and degradome sequencing reveal complex interactions between oilseed rape and the fungal pathogen Sclerotinia sclerotiorum(Lib.)

Integrated mRNA,sRNA,and degradome sequencing reveal complex...

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2017年中国作物学会学术年会

作者： hongju jian Jinqi Ma Lijuan Wei Pu Liu Aoxiang Zhang Bo Yang jiana Li Xinfu Xu Liezhao Liu Chongqing Engineering Research Center for Rapeseed College of Agronomy and BiotechnologySouthwest University

Sclerotinia stem rot(SSR),caused by the fungal pathogen Sclerotinia sclerotiorum,is a devastating disease resulting in yield losses and decreases in seed quality in oilseed rape(Brassica napus) ***,the molecular mechanisms underlying the response of oilseed rape to *** infection at the transcriptional and post-transcriptional levels are poorly *** this study,we used an integrated omics approach(transcriptome,sRNAome,and degradome sequencing) on the Illumina platform to compare the RNA expression and post-transcriptional profiles of oilseed rape plants inoculated or not with *** identified 7,065 differentially expressed genes(DEGs) compared with the mock-inoculated control at 48 hours post *** DEGs were associated with protein kinases,signal transduction,transcription factors,hormones,pathogenesis-related proteins,secondary metabolism,and *** the sRNA-Seq analysis,77 known and 176 novel miRNAs were identified;however,only 10 known and 41 novel miRNAs were differentially expressed between the samples inoculated or not with *** sequencing predicted 80 cleavage sites with 64 ***,55 DEGs and 15 miRNAs were verified by quantitative reverse transcription *** study provides a global view of miRNA and mRNA expression profiles in oilseed rape following *** infection.

关键词： Brassica napus Sclerotinia sclerotiorum transcnptome miRNAs degradome sequencing pathogens

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不同环境条件甘蓝型油菜含油量钝感系数的QTL分析

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基因组学与应用生物学 2014年第5期33卷 1075-1086页

作者：李超曲存民谌利荐红举李加纳贵州省油料研究所贵阳550006 西南大学农学与生物科技学院重庆400716 重庆市油菜工程技术研究中心重庆400716

本研究以甘蓝型油菜GH06和中油821为亲本杂交,后代通过＂一粒传法＂连续自交8代构建重组自交系群体SWU-2为材料,利用本实验室已构建的遗传连锁图谱和复合区间作图法（CIM）对三个不同环境下含油量及含油量环境钝感系数进行QTL分析。结... 详细信息

本研究以甘蓝型油菜GH06和中油821为亲本杂交,后代通过＂一粒传法＂连续自交8代构建重组自交系群体SWU-2为材料,利用本实验室已构建的遗传连锁图谱和复合区间作图法（CIM）对三个不同环境下含油量及含油量环境钝感系数进行QTL分析。结果表明：（1）不同环境中共检测到49个含油量相关QTL分别位于10个不同连锁群上,单个QTL可解释3.31%~12.47%的表型变异,42个含油量环境钝感系数QTL分别位于12个不同连锁群上,单个QTL可解释表型变异的4.05%~14.68%;（2）7个含油量相关的QTL与含油量钝感系数QTL相互重叠,分别位于第1、5、8、10、15和20连锁群上。研究结果表明明甘蓝型油菜含油量相关性状表现为多基因控制的数量性状,基因表达受环境影响较大,本研究为甘蓝型油菜含油量钝感基因的筛选提供了依据,有助于促进甘蓝型油菜高油稳油分子标记辅助育种的发展。

关键词：甘蓝型油菜含油量钝感系数数量性状座位

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The Hamming Distances of a Class of p-Ary Negacyclic Codes

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Chinese Journal of Electronics 2018年第1期27卷 46-51页

作者： DING jian LI hongju Department of General Education Anhui Xinhua University

Based on the construction of a new distance-preserving Gray map from(（F;+ uF;）;, Gray distance) to(the corresponding Gray images in F;, Hamming distance) and the calculation of Gray distances of（u-1）-constacyclic codes over F;+ uF;, a bound for the Hamming distances of a class of negacyclic codes with length p N over F;is obtained, which is more tighter than Singleton bound. Further more, the exact Hamming distances of some p-ary negacyclic codes are determined from this bound, some of which cannot be got from Dinh’s work published on Finite Fields and Their Applications in 2008.

关键词： Negacyclic code Hamming distance Gray distance Gray map

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Quantum Codes from Constacyclic Codes over Polynomial Residue Rings

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Chinese Journal of Electronics 2019年第6期28卷 1131-1138页

作者： DING jian LI hongju LIANG Jing TANG Yongsheng College of Mathematics and Informatics Fujian Normal University Fujian Provincial Key Lab of Network Security and Cryptology Fujian Normal University Department of General Education Anhui Xinhua University School of Mathematics and Statistics Hefei Normal University

Sufficient and necessary conditions for Hermitian（1 + λu）-constacyclic self-orthogonal codes over F;m +uF;m are obtained, where λ is a unit of F;m +uF;*** on this, a new method for the construction of p;ary quantum codes from the obtained Hermitian（1 + λu）-constacyclic self-orthogonal codes over F;m +uF;m is *** an example, [[p;-1, p;-2d + 1, d]];m quantum MDS codes are constructed for 1 ≤ d ≤（p;+ 1）/2 and p ≠ 2, as well as some other quantum codes.

关键词： Quantum code Quantum MDS code Constacyclic code Self-orthogonal code

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马铃薯CHI家族基因鉴定及其对外源水杨酸和茉莉酸的响应分析

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园艺学报 2021年第1期48卷 83-95页

作者：荐红举万梦圆付毅丁艺琚熙三尚丽娜李辉王季春胡柏耿吕典秋西南大学农学与生物科技学院南方山地农业教育部工程研究中心重庆400715 国家马铃薯工程技术研究中心山东德州253600

利用拟南芥几丁质酶(Chitinase,CHI)基因家族蛋白序列在马铃薯基因组内进行Blast P分析,获得马铃薯CHI家族成员,并对其进行基因结构分析、motif预测、进化树构建、顺式作用元件和组织表达分析。共有26个马铃薯CHI基因成员得到鉴定,其所... 详细信息

利用拟南芥几丁质酶(Chitinase,CHI)基因家族蛋白序列在马铃薯基因组内进行Blast P分析,获得马铃薯CHI家族成员,并对其进行基因结构分析、motif预测、进化树构建、顺式作用元件和组织表达分析。共有26个马铃薯CHI基因成员得到鉴定,其所有成员均含有较少的内含子,其中motif 1~motif 6均含有具有催化功能的GH19结构域。一共检测到166个胁迫或激素响应元件。进化树分析显示,CHI分为6个亚家族。马铃薯几丁质酶基因在不同组织中差异表达并且在水杨酸或茉莉酸处理后诱导表达,暗示水杨酸和茉莉酸对几丁质酶家族有不同的调控作用。

关键词：马铃薯几丁质酶基因水杨酸茉莉酸表达分析

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甘蓝型油菜含油率环境钝敏感基因型材料的筛选

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贵州农业科学 2014年第10期42卷 1-4页

作者：李超王璐璐杨斌饶勇肖华贵荐红举刘列钊李加纳贵州省油料研究所贵州贵阳550006 西南大学农学与生物科技学院重庆400716 重庆市油菜工程技术研究中心重庆400716

为了筛选含油率高、稳定性好、受环境影响小的甘蓝型油菜资源用于杂交油菜新品种选育,以含有219个家系的重组自交系SWU-2群体为材料,采取田间多年多点试验研究了不同环境条件对油菜含油率的影响。结果表明:SWU-2群体父本中油821的含油... 详细信息

为了筛选含油率高、稳定性好、受环境影响小的甘蓝型油菜资源用于杂交油菜新品种选育,以含有219个家系的重组自交系SWU-2群体为材料,采取田间多年多点试验研究了不同环境条件对油菜含油率的影响。结果表明:SWU-2群体父本中油821的含油率对环境较钝感,母本GH06含油率对环境较敏感;在SWU-2群体的219个家系中,有8个基因型材料的含油率对环境钝感,其含油率变化小于2百分点;有5个基因型材料对环境敏感,其含油率变化大于5百分点。SWU-2群体219个家系中的8个基因型材料可作为含油率稳定型资源用于甘蓝型杂交油菜新品种选育。

关键词：甘蓝型油菜含油率环境钝感敏感

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Ranks of Repeated-Root Constacyclic Code

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Wuhan University Journal of Natural Sciences 2016年第5期21卷 453-460页

作者： LI hongju DING jian TANG Yongsheng Department of Common Course Anhui Xinhua University School of Mathematics and Statistics Hefei Normal University

By the discussion of division in F2m[u]/〈u4〉,the minimal spanning set and the rank of a（1＋u＋u2）-constacyclic code with an arbitrary length N=2en over F2m[u]/〈u4〉 are determined based on the factorization of（x... 详细信息

关键词： constacyclic codes rank the minimal spanning set

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Fluid inclusion and H-O-S-Pb isotope systematics of the Chayong Cu-polymetallic deposit,Sanjiang Metallogenic Belt,Qinghai Province,China

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Acta Geochimica 2024年第6期43卷 1153-1168页

作者： jian Wang Hao Wang Fengyue Sun Fei Wang Xiangwen Li hongju Yue School of Mining Engineering Heilongjiang University of Science and TechnologyNo.2468 Puyuan StreetHarbin 150022People’s Republic of China Ang’angxi Physical Geological Data Field Observation and Research Station of Heilongjiang Province Qiqihar 161031People’s Republic of China College of Earth Science Jilin UniversityChangchun 130061People’s Republic of China The Six Geological Prospecting Institute of Heilongjiang Province Jiamusi 154002People’s Republic of China

The Chayong Cu-polymetallic deposit is a recently discovered Cu-polymetallic deposit hosted in the Sanjiang Metallogenic Belt within the Tibetan Plateau of China to the northeast of the North Qiangtang *** ore body occurs in siltstone and is controlled by a northwest-trending fault *** to the associations,assemblages,and cutting relationships between ore veins,the hydrothermal mineralization period can be divided into three mineralization stages:(1)a molybdenite mineralization stage,(2)a Cu-polymetallic sulfide stage,and(3)a quartzcarbonate *** types of fluid inclusions(FIs),namely,liquid and vapor-rich inclusions,are present in quartz as so ciated with sulfide ***-stage FIs are both iquid and vapor-rich,homogenized at temperatures ranging from 364.1 to 384.2℃,and have salinities ranging from0.70%to 9.60%NaCl equivalent(eqv).The middle-stage FIs are also both liquid-and vapor-rich,homogenized at temperatures ranging from 272.4 to 355.6℃,and have salinities ranging from 0.53%-17.10%NaCl *** late-stage FIs are liquid,homogenized at temperatures ranging from 209.4to 255.3℃,and have salinities ranging from 0.35%-6.87%NaCl *** samples from the deposit haveδ^(34)S values of-21.8‰to-19.2‰and-5.5‰to-6.0‰,suggesting that sulfur was derived from the host sediments and magmatic fluids,*** metallic minerals within the deposit have^(206)Pb/^(204)Pb,^(207)Pb/^(204)Pb,and^(208)Pb/^(204)Pb values of 18.439-18.458,15.656-15.679,and 38.772-38.863,respectively,suggesting that the metals were derived from the upper crust and orogenic *** samples from the deposit haveδ^(18)O_(W)values of 2.99‰-7.99‰andδD_(W) values ranging from-84.4‰to-73.9‰,indicating that the pre-forming fluids were magmatic and mixed with minor amounts of meteoric *** ore-forming fluid of the Chayong copper polymetallic deposit was a high-temperature,medium-to low-salinity H_(2)O-NaCl-CH_(4)-N_(2)±CO_(2)fluid *** early high-temperature m

关键词： Fluid inclusions S isotope Pb isotope H-O isotope Chayong Cu-polymetallic deposit Sanjiang Metallogenic Belt Yushu Qinghai

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马铃薯干旱相关microRNA的鉴定及其表达分析

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中国马铃薯 2023年第4期37卷 289-305页

作者：刘溶荣陈炜曦尹济容李姿燕王季春荐红举吕典秋西部(重庆)科学城种质创制大科学中心西南大学重庆400715 西南大学农学与生物科技学院重庆400715 西南大学南方山地农业教育部工程研究中心重庆400715 西南大学薯类生物学与遗传育种重庆市重点实验室重庆400715

马铃薯是世界第三大粮食作物,随着气候变暖以及淡水资源短缺,干旱成为制约其生长发育的重要因素。为了明确马铃薯响应干旱胁迫相关的microRNA(miRNA)及其靶基因的调控模式,利用small RNA测序技术、生物信息学方法和实时荧光定量PCR(qRT-... 详细信息

马铃薯是世界第三大粮食作物,随着气候变暖以及淡水资源短缺,干旱成为制约其生长发育的重要因素。为了明确马铃薯响应干旱胁迫相关的microRNA(miRNA)及其靶基因的调控模式,利用small RNA测序技术、生物信息学方法和实时荧光定量PCR(qRT-PCR)技术,对20%PEG6000模拟干旱处理0,1,3,6,12,24和48 h共7个时间点马铃薯组培幼苗的sRNA进行高通量测序、筛选和鉴定。从7个处理2个生物学重复共计14个样本中鉴定到621个miRNAs,包括308个已知miRNAs和313个新预测的miRNAs。其中250个已知miRNAs属于69个家族,166个新的miRNAs属于59个家族。miRNA长度分布在20~24 nt,其中主要集中在21和24 nt。差异表达分析共鉴定到40个差异表达的miRNAs,包括16个已知miRNAs和24个新的miRNAs。联合靶基因预测结果和干旱转录组数据,分别对16个已知和17个新miRNAs的81个和70个靶基因进行了功能注释,其主要参与生长代谢、胁迫响应、氧化还原反应及生物合成等过程;qRT-PCR分析结果与测序结果基本一致。研究为进一步挖掘马铃薯响应干旱胁迫的miRNA,以及阐明马铃薯中miRNA参与干旱响应的分子机制提供理论依据。

关键词：马铃薯干旱胁迫 small RNA测序 microRNA 靶基因 qRT-PCR

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