Integrated mRNA,sRNA,and degradome sequencing reveal complex interactions between oilseed rape and the fungal pathogen Sclerotinia sclerotiorum(Lib.)
作者单位：Chongqing Engineering Research Center for RapeseedCollege of Agronomy and BiotechnologySouthwest University
会议名称：《2017年中国作物学会学术年会》
会议日期：2017年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
关 键 词：Brassica napus Sclerotinia sclerotiorum transcnptome miRNAs degradome sequencing pathogens
摘 要：Sclerotinia stem rot(SSR),caused by the fungal pathogen Sclerotinia sclerotiorum,is a devastating disease resulting in yield losses and decreases in seed quality in oilseed rape(Brassica napus) ***,the molecular mechanisms underlying the response of oilseed rape to *** infection at the transcriptional and post-transcriptional levels are poorly *** this study,we used an integrated omics approach(transcriptome,sRNAome,and degradome sequencing) on the Illumina platform to compare the RNA expression and post-transcriptional profiles of oilseed rape plants inoculated or not with *** identified 7,065 differentially expressed genes(DEGs) compared with the mock-inoculated control at 48 hours post *** DEGs were associated with protein kinases,signal transduction,transcription factors,hormones,pathogenesis-related proteins,secondary metabolism,and *** the sRNA-Seq analysis,77 known and 176 novel miRNAs were identified;however,only 10 known and 41 novel miRNAs were differentially expressed between the samples inoculated or not with *** sequencing predicted 80 cleavage sites with 64 ***,55 DEGs and 15 miRNAs were verified by quantitative reverse transcription *** study provides a global view of miRNA and mRNA expression profiles in oilseed rape following *** infection.