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Horticultural Plant Journal 2023年第4期9卷 659-669页

作者： Tong li Xiao Zhang Yun Wei Yaxiu Xu Weiting liu hongjian li Guangxin Yang Aide Wang Xiaoxue Wang Rice Research Institute Shenyang Agricultural UniversityShenyangLiaoning 110866China College of Horticulture Shenyang Agricultural UniversityShenyangLiaoning 110866China liaoning Institute of Pomology XiongyueLiaoning115009China

Apple(Malus domestica)fruit generally undergoes a climacteric.During its ripening process,there is a peak in ethylene release and its firmness simultaneously decreases.Although more in-depth research into the mechanism of climacteric-type fruit ripening is being carried out,some aspects remain unclear.In this study,we compared the transcriptomes of 0-Pre and 15-Post(pre-and post-climacteric fruit),and 15-Post and 15-MCP[fruit treated with 1-MCP(1-methylcyclopropene)].Various transcription factors,such as MADS-box,ERF,NAC,Dof and SHF were identified among the DEGs(differential gene expressions).Furthermore,these transcription factors were selected for further validation analysis by qRT-PCR.Moreover,yeast one hybrid(Y1H),β-glucuronidase(GUS)transactivation assay and dual-luciferase reporter assay showed that MdAGL30,MdAGL104,MdERF008,MdNAC71,MdDof1.2,MdHSFB2a and MdHSFB3 bound to MdACS1 promoter and directly regulated its transcription,thereby regulating ethylene biosynthesis in apple fruit.Our results provide useful information and new insights for research on apple fruit ripening.

关键词： Apple RNA-Seq Fruit ripening Ethylene Transcription factor

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GL_(2)(Z)上的Catalan方程

数学进展 2024年第2期53卷 292-302页

作者：黎洪键袁平之华南师范大学数学科学学院广东广州510631

本文研究Catalan矩阵方程和另一个类似的矩阵方程在GL_(2)(Z)上的可解性,并且得到了它们在GL_(2)(Z)上的所有解.

关键词： Catalan方程 Catalan矩阵方程二次域的单位

MAPK通过调控TLR4/Myd88/NF-κB通路对妊娠期肠梗阻胃肠功能的影响

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实用医学杂志 2024年第8期40卷 1095-1100页

作者：高飞李景李洪健河北医科大学第二医院普外科石家庄050000 河北省第八人民医院护理部石家庄050000 河北省第八人民医院普外肛肠科石家庄050000

目的分析MAPK通过调控TLR4/MyD88/NF-κB通路对妊娠期肠梗阻胃肠功能的影响。方法选取105只SPF级Wistar大鼠,雌性、雄性分别为70、35只,将所有大鼠同笼放置后,共有33只雌性大鼠妊娠成功,将妊娠成功的8只作为空白组,剩余25只雌性大鼠... 详细信息

目的分析MAPK通过调控TLR4/MyD88/NF-κB通路对妊娠期肠梗阻胃肠功能的影响。方法选取105只SPF级Wistar大鼠,雌性、雄性分别为70、35只,将所有大鼠同笼放置后,共有33只雌性大鼠妊娠成功,将妊娠成功的8只作为空白组,剩余25只雌性大鼠建立肠梗阻模型,最终建模成功24只,将其分为模型组、上调组、下调组各8只。结果与模型组相比,上调组MAPK表达量、VIP、GAS、TNF-α、IL-6水平、TLR4、Myd88、NF-κB mRNA表达量及相对表达量上升,MOT、IL-4、IL-10水平下降,下调组MAPK表达量、VIP、GAS、TNF-α、IL-6水平、TLR4、Myd88、NF-κB mRNA表达量及相对表达量下降,MOT、IL-4、IL-10水平上升(P <0.05)。结论妊娠期肠梗阻大鼠经下调MAPK干预后胃肠功能改善,炎性反应减轻,其机制可能与TLR4/Myd88/NF-κB通路得到抑制有关。

关键词：妊娠期肠梗阻胃肠功能丝裂原活化蛋白激酶 Toll样受体4/髓样分化因子88/核转录因子-κB

Ultrawide dynamic modulation of perfect absorption with a Friedrich–Wintgen BIC

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Photonics Research 2023年第3期11卷 456-462页

作者： ENDUO GAO RONG JIN ZHENCHU FU GUANGTAO CAO YAN DENG JIAN CHEN GUANHAI li XIAOSHUANG CHEN hongjian li School of Physics and Electronics Central South UniversityChangsha 410083China State Key Laboratory of Infrared Physics Shanghai Institute of Technical PhysicsChinese Academy of SciencesShanghai 200083China School of Physics and Electronic Sciences Changsha University of Science and TechnologyChangsha 410004China School of Physics and Chemistry Hunan First Normal UniversityChangsha 410205China Hangzhou Institute for Advanced Study University of Chinese Academy of SciencesHangzhou 310024China

Dynamical control of perfect absorption plays an indispensable role in optical switch and modulators.However,it always suffers from the limited modulation range,small depth,and susceptible absorption efficiencies.Here,we propose a new strategy based on Friedrich–Wintgen bound states in the continuum(F–W BICs)to realize a tunable perfect absorber with large dynamic modulation range.For proof of concept,we demonstrate a pentaband ultrahigh absorption system consisting of graphene gratings and graphene sheets through elaborately tuning F–W BIC.The nature of the F–W BIC arises from the destructive interference between Fabry–Perot resonance and guided mode resonance modes in the coherent phase-matching condition.The radiation channels are avoided from crossing.The BIC can be dynamically modulated by engineering the Fermi level of graphene gratings,which breaks the traditional modulation methods with an incidence angle.Remarkably,the perfect absorber with this F–W BIC approach achieves the largest modulation range of up to 3.5 THz.We believe that this work provides a new way to dynamically engineer perfect absorption and stimulates the development of multiband ultracompact devices.

关键词： tuning absorption perfect

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网络直播带货新业态法律问题及规制

人民司法 2024年第4期 70-73页

作者：李红建江苏省连云港市中级人民法院

网络直播带货主要是指通过互联网站、应用程序、小程序等,以视频直播、音频直播、图文直播或多种直播相结合等形式开展营销的商业活动。当前,以平台经济、“互联网+”要素为典型的网络直播带货已经成为促进消费和经济增长的新兴业态。... 详细信息

网络直播带货主要是指通过互联网站、应用程序、小程序等,以视频直播、音频直播、图文直播或多种直播相结合等形式开展营销的商业活动。当前,以平台经济、“互联网+”要素为典型的网络直播带货已经成为促进消费和经济增长的新兴业态。江苏省连云港市作为国家“一带一路”强支点城市和中国(江苏)自由贸易试验区片区城市,高度重视电商经济发展,目前已经实现国家级、省级农村电商示范县全覆盖。

关键词：网络直播视频直播互联网站应用程序江苏省连云港市农村电商自由贸易试验区新业态

Genome-wide association analysis identified molecular markers associated with important tea flavor-related metabolites

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Horticulture Research 2021年第1期8卷 714-730页

作者： Kaixing Fang Zhiqiang Xia hongjian li Xiaohui Jiang Dandan Qin Qiushuang Wang Qing Wang Chendong Pan Bo li Hualing Wu Tea Research Institute Guangdong Academy of Agricultural SciencesGuangdong Key Laboratory of Tea Plant Resources Innovation&UtilizationGuangzhou 510640China Institute of Biotechnology Chinese Academy of Tropical Agricultural SciencesHaikou 570100China Hainan University Haikou 570228China

The characteristic secondary metabolites in tea(theanine,caffeine,and catechins)are important factors contributing to unique tea flavors.However,there has been relatively little research on molecular markers related to these metabolites.Thus,we conducted a genome-wide association analysis of the levels of these tea flavor-related metabolites in three seasons.The theanine,caffeine,and catechin levels in Population 1 comprising 191 tea plant germplasms were examined,which revealed that their heritability exceeded 0.5 in the analyzed seasons,with the following rank order(highest to lowest heritabilities):(+)-catechin>(−)-gallocatechin gallate>caffeine=(−)-epicatechin>(−)-epigallocatechin-3-gallate>theanine>(−)-epigallocatechin>(−)-epicatechin-3-gallate>catechin gallate>(+)-gallocatechin.The SNPs detected by amplified-fragment SNP and methylation sequencing divided Population 1 into three groups and seven subgroups.An association analysis yielded 307 SNP markers related to theanine,caffeine,and catechins that were common to all three seasons.Some of the markers were pleiotropic.The functional annotation of 180 key genes at the SNP loci revealed that FLS,UGT,MYB,and WD40 domain-containing proteins,as well as ATP-binding cassette transporters,may be important for catechin synthesis.KEGG and GO analyses indicated that these genes are associated with metabolic pathways and secondary metabolite biosynthesis.Moreover,in Population 2(98 tea plant germplasm resources),30 candidate SNPs were verified,including 17 SNPs that were significantly or extremely significantly associated with specific metabolite levels.These results will provide a foundation for future research on important flavor-related metabolites and may help accelerate the breeding of new tea varieties.

关键词： analysis breeding Population

Triple plasmon-induced transparency and outstanding slow-light in quasi-continuous monolayer graphene structure

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Science China(Physics,Mechanics & Astronomy) 2021年第2期64卷 61-71页

作者： CuiXiu Xiong Hui Xu MingZhuo Zhao BaiHui Zhang Chao liu Biao Zeng Kuan Wu BanXian Ruan Min li hongjian li School of Physics and Electronics Central South UniversityChangsha410083China All-solid-state Energy Storage Materials and Devices Key Laboratory of Hunan Province College of Information and Electronic EngineeringHunan City UniversityYiyang413000China School of Mathematics and Statistics Hunan University of Technology and BusinessChangsha410205China

We propose a simple quasi-continuous monolayer graphene structure and achieve a dynamically tunable triple plasmon-induced transparency(PIT)effect in the proposed structure.Additional analyses indicate that the proposed structure contains a selfconstructed bright-dark-dark mode system.A uniform theoretical model is introduced to investigate the spectral response characteristics and slow light-effects in the proposed system,and the theoretical and the simulated results exhibit high consistency.In addition,the influences of the Fermi level and the carrier mobility of graphene on transmission spectra are discussed.It is found that each PIT window exhibits an independent dynamical adjustability owing to the quasi-continuity of the proposed structure.Finally,the slow-light effects are investigated based on the calculation of the group refractive index and phase shift.It is found that the structure displays excellent slow-light effects near the PIT windows with high-group indices,and the maximum group index of each PIT window exceeds 1000 when the carrier mobility of graphene increases to 3.5 m^2 V^-1s^-1.The proposed structure has potential to be used in multichannel filters,optical switches,modulators,and slow-light devices.Additionally,the established theoretical model lays a theoretical basis for research on multimode coupling effects.

关键词： monolayer graphene structure plasmon-induced transparency slow light

Genetic improvement of heat tolerance in wheat:Recent progress in understanding the underlying molecular mechanisms

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The Crop Journal 2018年第1期6卷 32-41页

作者： Zhongfu Ni hongjian li Yue Zhao Huiru Peng Zhaorong Hu Mingming Xin Qixin Sun State Key Laboratory for Agrobiotechnology Key Laboratory of Crop Heterosis and Utilization Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University

As a cool season crop, wheat(Triticum aestivum L.) has an optimal daytime growing temperature of 15 ℃ during the reproductive stage. With global climate change, heat stress is becoming an increasingly severe constraint on wheat production. In this review, we summarize recent progress in understanding the molecular mechanisms of heat tolerance in wheat. We firstly describe the impact of heat tolerance on morphology and physiology and its potential effect on agronomic traits. We then review recent discoveries in determining the genetic and molecular factors affecting heat tolerance, including the effects of phytohormone signaling and epigenetic regulation. Finally, we discuss integrative strategies to improve heat tolerance by utilization of existing germplasm including modern cultivars, landraces and related species.

关键词： Heat stress Phytohormone signaling Epigenetic regulation Triticum aestivum

科技创新领军人才阶段性成长规律与启示——基于2017年科技创新领军人才履历分析

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中国科技人才 2024年第2期 9-18页

作者：吴邦正李洪坚河北金融学院河北保定071051 中国劳动关系学院北京100048

科技创新领军人才是推动原始创新、技术突破和颠覆性创新的核心力量。本文从科技创新领军人才成长的阶段性视角,梳理科技创新领军人才各阶段发展的典型特征,从基础教育阶段、高等教育阶段、评前工作阶段以及评后工作阶段总结成长规律,... 详细信息

科技创新领军人才是推动原始创新、技术突破和颠覆性创新的核心力量。本文从科技创新领军人才成长的阶段性视角,梳理科技创新领军人才各阶段发展的典型特征,从基础教育阶段、高等教育阶段、评前工作阶段以及评后工作阶段总结成长规律,从基础教育水平、教育背景多元化、丰富的工作经历与科研经验以及用好用活科技创新领军人才方面提出具体建议。

关键词：科技创新领军人才人才培养人才成长

Flexible dopamine-sensing fiber based on potentiometric method for long-term detection in vivo

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Science China Chemistry 2021年第10期64卷 1763-1769页

作者： liyuan Wang Jiawei Chen Jiajia Wang hongjian li Chuanrui Chen Jianyou Feng Yue Guo Hongbo Yu Xuemei Sun Huisheng Peng State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular Scienceand Laboratory of Advanced MaterialsFudan UniversityShanghai 200438China Vision Research Laboratory School of Life SciencesState Key Laboratory of Medical NeurobiologyCollaborative Innovation Centre for Brain ScienceFudan UniversityShanghai 200438China

Achieving real-time,continuous and long-term monitoring of dopamine(DA)in vivo is essential for revealing brain functions and preventing and treating neurogenic diseases.However,it remains challenging to achieve a low limit of detection(LOD)and high neuron-compatibility at the same time for the current microsensors,resulting in the failure of long-term and accurate detection of DA in vivo.A DA-sensing fiber was achieved by the potentiometric method to possess a low LOD of 5 nM,1-3 orders of magnitude lower than amperometry and differential-pulse voltammetry.The sensing fiber showed a wide linear range from 5 to 185 nM that well matched the DA concentration(26-40 nM)in vivo.After implantation,the sensing fiber showed no influence on the firing rates of neurons with the potentiometric test,indicating high neuron-compatibility.It was then integrated with electrophysiology to simultaneously monitor DA variation and electrical signal in the brain,with stable monitoring of DA change in vivo for 8 weeks.The sensing fiber was flexible and stably worked after hundreds of bending,and it showed high sensitivity even after protein adsorption,thus offering a reliable tool for neuroscience.

关键词： carbon nanotube fiber potentiometric implantable long-term dopamine detection