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Photochemical hydrogen production with molecular devices comprising a zinc porphyrin and a cobaloxime catalyst

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Science China Chemistry 2012年第7期55卷 1274-1282页

作者： ZHANG Pan WANG Mei LI XueQiang cui hongguang DONG JingFeng SUN LiCheng State Key Laboratory of Fine Chemicals DUT-KTIt Joint Education and Research Centre on Molecular Devices Dalian University of Technology(DUT)Dalian 116012China College of Environmental and Chemical Engineering Dalian UniversityDalian 116622China Department of Chemistry Royal Institute of Technology(KTH)Stockholm 10044Sweden

Two new noble-metal-free molecular devices, [{Co（dmgH）2Cl}{Zn（PyTPP）}] （1, dmgH = dimethyloxime, PyTPP = 5-（4- pyridyl）-10,15,20-triphenylporphyrin） and [{Co（dmgH）2Cl}{Zn（apPyTPP）}] （2, apPyTPP = 5-[4-（isonicotinamidyl）phenyl]- 10,15,20-triphenylporphyrin）, for light-driven hydrogen generation were prepared and spectroscopically characterized. The zinc porphyrin photosensitizer and the Co III-based catalyst unit are linked by axial coordination of a pyridyl group in the periphery of zinc-porphyrin to the cobalt centre of catalyst with different lengths of bridges. The apparent fluorescence quenching and lifetime decays of 1 and 2 were observed in comparison with their reference chromophores, Zn（PyTPP）（3） and Zn（apPyTPP）（4）, suggesting a possibility for an intramolecular electron transfer from the singlet excited state of zinc porphyrin unit to the cobalt centre in the molecular devices. Photochemical H2-evolving studies show that complexes 1 and 2 are efficient molecular photocatalysts for visible light-driven H2 generation from water with triethylamine as a sacrificial electron donor in THF/H20, with turnover numbers up to 46 and 35 for 1 and 2, respectively. In contrast to these molecular devices, the multicomponent catalyst of zinc porphyrin and [Co（dmgH）2PyCl] did not show any fluorescence quenching and as a consequence, no H2 gas was detected by GC analysis in the presence of triethylamine with irradiation of visible light. The plausible mechanism for the photochemical H2 generation with these molecular devices is discussed.

关键词： cobaloxime hydrogen production molecular device photocatalysis zinc porphyrin

SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants

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Molecular Plant 2023年第3期16卷 632-642页

作者： Linhao Ge Buwei Cao Rui Qiao hongguang cui Shaofang Li Hongying Shan Pan Gong Mingzhen Zhang Hao Li Aiming Wang Xueping Zhou Fangfang Li State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBejingChina Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests Ministry of Education and College of Plant ProtectionHainan UniversityHaikouHainanChina London Research and Development Centre Agriculture and Agri-Food CanadaLondonONCanada Department of Biology Western UniversityLondonONCanada state Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhouZhejiangChina

RNA quality control nonsense-mediated decay is involved in viral restriction in both plants and ***,it is not known whether two other RNA quality control pathways,nonstop decay and no-go decay,are capable of restricting viruses in ***,we show that the evolutionarily conserved Pelota–Hbs1 complex negatively regulates infection of plant viruses in the family Potyviridae(termed potyvirids),the largest group of plant RNA viruses that accounts for more than half of the viral crop damage *** enables the recognition of the functional G1-2A6-7 motif in the P3 cistron,which is conserved in almost all *** allows Pelota to target the virus and act as a viral restriction ***,Pelota interacts with the SUMO E2-conjugating enzyme SCE1 and is SUMOylated in *** Pelota SUMOylation disrupts the ability to recruit Hbs1 and inhibits viral RNA *** findings reveal the functional importance of Pelota SUMOylation during the infection of potyvirids in plants.

关键词： SUMOylation Pelota-Hbs1 G1-2A6-7 motif Potyviruses

An efficient papaya leaf distortion mosaic potyvirus vector for virus-induced gene silencing in papaya

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Horticulture Research 2021年第1期8卷 2095-2106页

作者： Decai Tuo Pu Yan Guangyuan Zhao hongguang cui Guopeng Zhu Yang Liu Xiukun Yang He Wang Xiaoying Li Wentao Shen Peng Zhou Key Laboratory of Biology and Genetic Resou rces of Tropical Crops Min ist ry of Agriculture and Rural Affairs&Institute of Tropical Bioscienee and BiotechnologyChinese Academy of Tropical Agricultural Sciences571101 HaikouChina Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources&Institute for Tropical Agricultural Resources Chinese Academy of Tropical Agricultural Sciences571101 HaikouChina College of Plant Protection Hainan University570228 HaikouChina College of Horticulture Hainan University570228 HaikouChina Hainan Key Laboratory of Tropical Microbe Resources 571101 HaikouChina

Papaya(Carica papaya L.)is regarded as an excellent model for genomic studies of tropical trees because of its short generation time and its small genome that has been ***,functional genomic studies in papaya depend on laborious genetic transformations because no rapid tools exist for this ***,we developed a highly efficient virus-induced gene silencing(VIGS)vector for use in papaya by modifying an artificially attenuated infectious clone of papaya leaf distortion mosaic virus(PLDMV;genus:Potyvirus),PLDMV-E,into a stable Nimble Cloning(NC)-based PLDMV vector,pPLDMV-NC,in Escherichia *** target fragments for gene silencing can easily be cloned into pPLDMV-NC without multiple digestion and ligation *** this PLDMV VIGS system,we silenced and characterized five endogenous genes in papaya,including two common VIGS marker genes,namely,phytoene desaturase,Mg-chelatase H subunit,putative GIBBERELLIN(GA)-INSENSITIVE DWARF1A and 1B encoding GA receptors;and the cytochrome P450 gene CYP83B1,which encodes a key enzyme involved in benzylglucosinolate *** results demonstrate that our newly developed PLDMV VIGS vector is a rapid and convenient tool for functional genomic studies in papaya.

关键词： species papaya VIGS

First report of Fusarium equiseti causing shoot blight on Moringa oleifera in China

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First report of Fusarium equiseti causing shoot blight on Mo...

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Jin Pengfei Kang Xun cui hongguang Liu Wenbo Miao Weiguo Institute of Tropical Agriculture and Foresty Hainan University

Moringa oleifera,a perennial arbor tree,is widely grown in tropical and sub-tropical areas owing to its ornamental value and economic *** November 2015,a new disease that caused the stems of *** to brown and die was found in Changjiang,Hainan Province,*** diseasing trees initially showed water-soaked brown spots on biennial stems that merged soon,then twigs and branches withered and leaves defoliated,and finally trees *** branches were sampled for pathogen ***,diseasing tissue was surface disinfected with 75%ethanol solution(V/V)for 30 sec,soaked in 0.1%HgCl for 1 min,followed by rinsing four times in sterile distilled *** isolate was darkness sub-cultured on potato dextrose agar(PDA) at(28±0.5) ℃.Typical growth characteristics observed were development of abundant white aerial mycelium that turned peach orange by incubating under ***,morphological characteristics of conidiophores and conidia were determined,and the results showed conidiophores with the color of pale or light brown were straight to slightly curved,unbranched and *** were single-celled,hyaline,non-septate and ovoid,with the size ranging from 8.5～11.3 × 1.6～5.2μ*** with the size ranging from 16.5～45 ×1.6～5.5μm were mostly two-to five-septate and slightly curved at *** were produced in hyphae,most often intercalary,solitary,in pairs,frequently forming chains or clusters,globose(6～10μm long).Single-spore cultures were obtained and identified as Fusarium equiseti on the basis of morphological and physiological *** DNA was extracted from the culture,the internal transcribed spacer(ITS) regions of the rDNA was amplified using primers ITS1 and ITS4(White et al.,1990),and the resulting 545 bp amplicon was *** against NCBI GenBank database revealed that the sequence of the isolate identified in this study shared 100%similarity with that of *** isolate MO 157

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Analysis of the complete genome sequence of a potyvirus from passion fruit suggests its taxonomic classification as a member of a new species

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Analysis of the complete genome sequence of a potyvirus from...

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Yang Ke Jin Pengfei Miao Weiguo Zheng Li cui hongguang College of Tropical Agriculture and Forestry Hainan University

The complete genomic sequence of a telosma mosaic virus(TeMV) isolate(named PasFru),identified in passion fruit in China,was *** entire RNA genome of PasFru comprises 10 049 nucleotides(nt) excluding the poly(A) tailand encodes a polyprotein of 3 173 amino acids(aa),flanked by 5’ and 3’ untranslated regions(UTR) of 276 nt and 251 nt,*** with the previous TeMV isolate Hanoi from Telosma cordata,the only documented isolate with theentire genome sequence annotated,PasFru had an extra 87 nt and 89 aa residues at the 3’-end of5’UTR and the N-terminusof the P1 protein,respectively,which contributed to the genome size difference between PasFru and Hanoi(10 049 nt versus 9 689 nt).Pairwise sequence comparisons showed that PasFru shares 73.6%nt and 80.9%aa sequence identity with the Hanoiisolate at the whole-genome and polyprotein level,respectively,and these values are below the corresponding thresholdvalues for species demarcation in the family *** data suggest that TeMV-PasFru should be classified as anew member of the genus Potyvirus.

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Complete genomic sequenceand organization of a novel mycovirus from Phoma matteuciicola strain LG915

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Complete genomic sequenceand organization of a novel mycovir...

中国植物病理学会2019年学术年会

作者： Zheng Fan Xu Gang Zhou Jia Xie Changping cui hongguang Miao Weiguo Kang Zhensheng Zheng Li College of Plant Protection Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and PestsMinistry of Education State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University

The complete genome of a double-stranded RNA(dsRNA) mycovirus,Phoma matteuciicola partitivirus 1(PmPV1) was *** consists of two dsRNA segments with 1 664 bp(dsRNA-1) and 1 383 bp(dsRNA-2) in length and each containing a single open reading frame(ORF)and potentially encoded 46.78 ku and 40.92 ku proteins,***-1 encodes a putative polypeptide with a conserved RNA—dependent RNA polymerase(RdRp) domain and showed similarity to that of *** protein encoded by dsRNA-2 has no significant similarity to the typical coat protein(CP) of the partitiviruses,while the structure analysis further suggested that might have the hypothetical function of coat *** viral particles of PmPV 1 were isometric and approximately 29 nm in *** analysis showed that PmPV1 is closely related to the Gammapartitivirus within the family Partitiviridae,but forming a separate branch with Colletotrichum acutatum RNA virus 1 and Ustilaginoidea virens partitivirus *** is the first report of the full-length nucleotide sequence of a novel virus classified in the genus Gammapartitivirus,infecting *** strain LG915,the causal agent of leaf blight of Curcuma wenyujin.

关键词： Phoma matteuciicola Mycovirus Gammapartitivirus Partitiviridae

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改性玉米淀粉黏结剂对种绳捻制质量的影响

农业工程学报 2010年第5期26卷 164-169页

作者：任文涛代丽丽崔红光向权力沈阳农业大学工程学院沈阳110866

为了提高水稻种绳的抗拉强度和穴距准确性、防止种子在种绳捻制和直播过程中脱落,提出了在包容材料上涂刷黏结剂的方法并配制了一种改性玉米淀粉黏结剂。试验结果表明,配制的改性玉米淀粉黏结剂及其涂刷量和黏度对水稻出苗率影响不显著... 详细信息

为了提高水稻种绳的抗拉强度和穴距准确性、防止种子在种绳捻制和直播过程中脱落,提出了在包容材料上涂刷黏结剂的方法并配制了一种改性玉米淀粉黏结剂。试验结果表明,配制的改性玉米淀粉黏结剂及其涂刷量和黏度对水稻出苗率影响不显著。通过二次回归正交旋转组合试验,研究了改性玉米淀粉黏结剂的涂刷量和黏度与水稻种绳的抗拉强度、穴距准确性和每穴粒数合格率的关系,建立了相应的数学模型。利用多目标非线性优化方法,对黏结剂的涂刷量和黏度进行了参数优化。确定的最优参数组合是黏结剂涂刷量为80%、黏度为7792mPa·s。在此条件下,种绳的抗拉强度达到18.84N,穴距变异系数为3.25%,穴粒数合格率为91.05%。研究结果证明,在种绳捻制过程中涂刷适量的改性玉米淀粉黏结剂,对提高种绳抗拉强度和穴距准确性具有重要作用,对水稻出苗和土壤环境没有影响。这为水稻种绳捻制机的改进设计提供了重要参考。

关键词：抗拉强度黏结剂黏度种绳玉米淀粉出苗率穴距准确性穴粒数合格率

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基于时域有限差分的微放电仿真算法

中国空间科学技术 2017年第2期37卷 89-95页

作者：张剑锋游检卫王洪广李韵崔万照崔铁军东南大学毫米波国家重点实验室南京210096 西安交通大学电子物理与器件研究所西安710049 中国空间技术研究院西安分院空间微波技术重点实验室西安710110

为克服传统微放电阈值预测方法建模粗糙、精度低的缺点,提高阀值预测精度和效率,研究了基于时域有限差分的精确微放电阀值预测算法。基于时域有限差分算法和粒子追踪算法,通过时空网格自洽互耦实现复杂结构微波器件微放电阈值准确计算... 详细信息

为克服传统微放电阈值预测方法建模粗糙、精度低的缺点,提高阀值预测精度和效率,研究了基于时域有限差分的精确微放电阀值预测算法。基于时域有限差分算法和粒子追踪算法,通过时空网格自洽互耦实现复杂结构微波器件微放电阈值准确计算。其中,共形和并行算法是提高微放电仿真精度和效率的关键。文章基于空间蛙跳策略实现了基于时域有限差分的微放电仿真算法,利用算法分析了典型微波器件的微放电阈值,仿真与实验结果吻合良好,误差小于0.3dB;同时,并行效率最高可达83%,验证了算法的准确性和高效性。

关键词：时域有限差分粒子追踪微放电共形并行

Bacillus atrophaeus strain HAB-5 promotes vegetative growth of tobacco and its secretion metabolites protect plants against tobacco mosaic virus infection

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Bacillus atrophaeus strain HAB-5 promotes vegetative growth ...

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Mamy Jayne Nelly Rajaofera Jin Pengfei Shen Haiyan Hafiz Hasnain Nawaz Wang Yi He Qiguang Xu Liangxiang cui hongguang Liu Wenbo Miao Weiguo Institute of Tropical Agriculture and Forestry Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresource

Finding alternatives of synthetic pesticide for health and the safe environment has become a crucial issue for scientific research.A number of studies have reported efficacy of Bacillus species on promoting plant development,as well as protecting plants against pathogens invasion,especially pathogenic fungi and ***,little was known about Bacillus species in controlling viral *** this study,Bacillus atrophaeus strain HAB-5,isolated from soil of cotton field,Xinjiang,China,was able to efficiently promote the growth of tobacco plant in that the expansins genes NtEXPl and NtEXP2 in tobacco leaves after treatment with *** Tobacco mosaic virus(TMV)/Nicotiana tobacco system was employed to evaluate virus resistance induced by *** leaves were treated with antimicrobial metabolites of HAB-5 strain(lmg/mL),and 12 hours later the treated leaves were challenged with TMV via *** results showed that diseasing symptoms were obviously compromised in tobacco leaves treated with HAB-5,and viral accumulation levels were also greatly ***,it was found that the signaling regulatory gene(NPR1),defense genes(PR-1a,PR-1b,Chia5),and hypertensive response related genes(Hsr203J,Hinl) were up-regulated in plants treated with the ***,these accumulated results strongly supported the strain HAB-5 to be a biocontrol strain against TMV.

关键词： Bacillus atrophaeus Tobacco mosaic virus Biocontrol