First report of Fusarium equiseti causing shoot blight on Moringa oleifera in China
作者单位：Institute of Tropical Agriculture and ForestyHainan University
会议名称：《中国植物病理学会第十一届全国会员代表大会暨2018年学术年会》
会议日期：2018年
基 金：supported by he Scientific Research Foundation for Advanced Talents,Hainan University[No.KYQD(ZR)1842] the Production-teaching-research integration project of Hainan(No.CXY20140038) International Science&Technology Cooperation Program of Hainnan(No.ZDYF2016208) the Nature Science Foundation of China(No.31360029)
摘 要：Moringa oleifera,a perennial arbor tree,is widely grown in tropical and sub-tropical areas owing to its ornamental value and economic *** November 2015,a new disease that caused the stems of *** to brown and die was found in Changjiang,Hainan Province,*** diseasing trees initially showed water-soaked brown spots on biennial stems that merged soon,then twigs and branches withered and leaves defoliated,and finally trees *** branches were sampled for pathogen ***,diseasing tissue was surface disinfected with 75%ethanol solution(V/V)for 30 sec,soaked in 0.1%HgCl for 1 min,followed by rinsing four times in sterile distilled *** isolate was darkness sub-cultured on potato dextrose agar(PDA) at(28±0.5) ℃.Typical growth characteristics observed were development of abundant white aerial mycelium that turned peach orange by incubating under ***,morphological characteristics of conidiophores and conidia were determined,and the results showed conidiophores with the color of pale or light brown were straight to slightly curved,unbranched and *** were single-celled,hyaline,non-septate and ovoid,with the size ranging from 8.5～11.3 × 1.6～5.2μ*** with the size ranging from 16.5～45 ×1.6～5.5μm were mostly two-to five-septate and slightly curved at *** were produced in hyphae,most often intercalary,solitary,in pairs,frequently forming chains or clusters,globose(6～10μm long).Single-spore cultures were obtained and identified as Fusarium equiseti on the basis of morphological and physiological *** DNA was extracted from the culture,the internal transcribed spacer(ITS) regions of the rDNA was amplified using primers ITS1 and ITS4(White et al.,1990),and the resulting 545 bp amplicon was *** against NCBI GenBank database revealed that the sequence of the isolate identified in this study shared 100%similarity with that of *** isolate MO 157