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Human umbilical cord mesenchymal stem cell-derived exosomes loaded into a composite conduit promote functional recovery after peripheral nerve injury in rats

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Neural Regeneration Research 2024年第4期19卷 900-907页

作者： Haoshuai Tang Junjin Li hongda wang Jie Ren Han Ding Jun Shang Min wang Zhijian Wei Shiqing Feng Department of Othopaedics Tianjin Medical University General Hospital International Science and Technology Cooperation Base of Spinal Cord Injury Tianjin Key Laboratory of Spine and Spinal Cord InjuryDepartment of OrthopedicsTianjin Medical University General Hospital Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment Tianjin Lung Cancer InstituteTianjin Medical University General Hospital Department of Othopaedics Qilu Hospital of Shandong UniversityShandong University Centre for OthopedicsAdvanced Medical Research InstituteShandong University Orthopedic Research Center of Shandong University&Advanced Medical Research Institute Cheeloo College of MedicineShandong University

Complete transverse injury of peripheral nerves is challenging to treat.Exosomes secreted by human umbilical cord mesenchymal stem cells are considered to play an important role in intercellular communication and regulate tissue regeneration.In previous studies,a collagen/hyaluronic acid sponge was shown to provide a suitable regeneration environment for Schwann cell proliferation and to promote axonal regeneration.This three-dimensional(3D)composite conduit contains a collagen/hyaluronic acid inner sponge enclosed in an electrospun hollow poly(lactic-co-glycolic acid)tube.However,whether there is a synergy between the 3D composite conduit and exosomes in the repair of peripheral nerve injury remains unknown.In this study,we tested a comprehensive strategy for repairing long-gap(10 mm)peripheral nerve injury that combined the 3D composite conduit with human umbilical cord mesenchymal stem cell-derived exosomes.Repair effectiveness was evaluated by sciatic functional index,sciatic nerve compound muscle action potential recording,recovery of muscle mass,measuring the cross-sectional area of the muscle fiber,Masson trichrome staining,and transmission electron microscopy of the regenerated nerve in rats.The results showed that transplantation of the 3D composite conduit loaded with human umbilical cord mesenchymal stem cell-derived exosomes promoted peripheral nerve regeneration and restoration of motor function,similar to autograft transplantation.More CD31-positive endothelial cells were observed in the regenerated nerve after transplantation of the loaded conduit than after transplantation of the conduit without exosomes,which may have contributed to the observed increase in axon regeneration and distal nerve reconnection.Therefore,the use of a 3D composite conduit loaded with human umbilical cord mesenchymal stem cell-derived exosomes represents a promising cell-free therapeutic option for the treatment of peripheral nerve injury.

关键词： axon growth collagen exosome human umbilical cord mesenchymal stem cells hyaluronic acid muscular atrophy nerve guidance conduits peripheral nerve regeneration

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An Algorithm for Target Detection of Engineering Vehicles Based on Improved CenterNet

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Computers, Materials & Continua 2022年第11期73卷 4261-4276页

作者： Pingping Yu hongda wang Xiaodong Zhao Guangchen Ruan School of Information Science and Engineering Hebei University of Science and TechnologyShijiazhuang050000China Indiana University at Bloomington Indiana47405USA

Aiming at the problems of low target image resolution,insufficient target feature extraction,low detection accuracy and poor real time in remote engineering vehicle detection,an improved CenterNet target detection model is proposed in this paper.Firstly,EfficientNet-B0 with Efficient Channel Attention(ECA)module is used as the basic network,which increases the quality and speed of feature extraction and reduces the number of model parameters.Then,the proposed Adaptive Fusion Bidirectional Feature Pyramid Network(AF-BiFPN)module is applied to fuse the features of different feature layers.Furthermore,the feature information of engineering vehicle targets is added by making full use of the high-level semantic and low-level fine-grained feature information of the target,which overcomes the problem that the original CenterNet network did not perform well in small target detection and improve the detection accuracy of the network.Finally,the tag coding strategy and bounding box regression method of CenterNet are optimized by introducing positioning quality loss.The accuracy of target prediction is increased by joint prediction of center position and target size.Experimental results show that the mean Average Precision(mAP)of the improved CenterNet model is 94.74%on the engineering vehicle dataset,and the detection rate is 29 FPS.Compared with the original CenterNet model based on ResNet-18,the detection accuracy of this model is improved by 16.29%,the detection speed is increased by 9 FPS,and the memory usage is reduced by 43 MB.Compared with YOLOv3 and YOLOv4,the mAP of this model is improved by 19.9%and 5.61%respectively.The proposed method can detect engineering vehicles more quickly and accurately in far distance.It has obvious advantages in target detection compared with traditional methods.

关键词： Engineering vehicle target detection CenterNet feature fusion

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Cell membrane sample preparation method of combined AFM and dSTORM analysis

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Biophysics Reports 2022年第4期8卷 183-192页

作者： Mingjun Cai Huili wang Guanfang Zhao Hongru Li Jing Gao hongda wang University of Science and Technology of China State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology (Qingdao)

A major role of cell membranes is to provide an ideal environment for the constituent proteins to perform their biological functions. A deep understanding of the membrane proteins assembly process under physiological conditions is quite important to elucidate both the structure and the function of the cell membranes. Along these lines, in this work, a complete workflow of the cell membrane sample preparation and the correlated AFM and dSTORM imaging analysis methods are presented. A specially designed, angle-controlled sample preparation device was used to prepare the cell membrane samples.The correlated distributions of the specific membrane proteins with the topography of the cytoplasmic side of the cell membranes can be obtained by performing correlative AFM and dSTORM measurements. These methods are ideal for systematically studying the structure of the cell membranes. The proposed method of the sample characterization was not only limited to the measurement of the cell membrane but also can be applied for both biological tissue section analysis and detection.

关键词： Atomic force microscopy (AFM) Super-resolution microscopy (SRM) Direct stochastic optical reconstruction microscopy (dSTORM) Cell membrane Combined technique

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Dimension-Enhanced Ultra-High Performance Liquid Chromatography/Ion Mobility-Quadrupole Time-of-Flight Mass Spectrometry Combined with Intelligent Peak Annotation for the Rapid Characterization of the Multiple Components from Seeds of Descurainia sophia

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Phyton-International Journal of Experimental Botany 2022年第3期91卷 541-567页

作者： Simiao wang Xue Li Boxue Chen Shitong Li Jiali wang Jing wang Mingshuo Yang Xiaoyan Xu hongda wang Wenzhi Yang State Key Laboratory of Component-Based Chinese Medicine Tianjin Key Laboratory of TCM Chemistry and AnalysisTianjin University of Traditional Chinese MedicineTianjin301617China Waters Technology Co. Ltd.Beijing101102China School of Computing Science University of GlasgowGlasgowG128RZUK

The complex composition of herbal metabolites necessitates the development of powerful analytical techniques aimed to identify the bioactive components.The seeds of Descurainia sophia(SDS)are utilized in China as a cough and asthma relieving agent.Herein,a dimension-enhanced integral approach,by combining ultra-high performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry(UHPLC/IMQTOF-MS)and intelligent peak annotation,was developed to rapidly characterize the multicomponents from SDS.Good chromatographic separation was achieved within 38 min on a UPLC CSH C18(2.1×100 mm,1.7μm)column which was eluted by 0.1%formic acid in water(water phase)and acetonitrile(organic phase).Collision-induced dissociation-MS^(2)data were acquired by the data-independent high-definition MS^(E)(HDMS^(E))in both the negative and positive electrospray ionization modes.A major components knockout strategy was applied to improve the characterization of those minor ingredients by enhancing the injection volume.Moreover,a self-built chemistry library was established,which could be matched by the UNIFI software enabling automatic peak annotation of the obtained HDMS^(E)data.As a result of applying the intelligent peak annotation workflows and further confirmation process,a total of 53 compounds were identified or tentatively characterized from the SDS,including 29 flavonoids,one uridine derivative,four glucosides,one lignin,one phenolic compound,and 17 others.Notably,four-dimensional information related to the structure(e.g.,retention time,collision cross section,MS^(1)and MS^(2)data)was obtained for each component by the developed integral approach,and the results would greatly benefit the quality control of SDS.

关键词： Descurainia sophia multicomponent characterization ultra-high performance liquid chromatography ion mobility/quadrupole time-of-flight mass spectrometry high-definition MS^(E) flavonoid

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Rapid Profiling and Characterization of the Multicomponents from the Root and Rhizome of Salvia miltiorrhiza by Ultra-High Performance Liquid Chromatography/Ion Mobility-Quadrupole Time-of-Flight Mass Spectrometry in Combination with Computational Peak Annotation Workflows

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Phyton-International Journal of Experimental Botany 2022年第5期91卷 1073-1088页

作者： Boxue Chen hongda wang Meiyu Liu Wandi Hu Yuexin Qian Jiali wang Jie Liu Xue Li Jing wang Wenzhi Yang State Key Laboratory of Component-Based Chinese Medicine&Tianjin Key Laboratory of TCM Chemistry and Analysis Tianjin University of Traditional Chinese MedicineTianjin301617China Waters Technology Co. Ltd.Beijing101102China

Herbal components characterization represents a challenging task because of the co-existing of multiple classes of naturally occurring compounds with wide spans of polarity,molecular mass,and the ubiquitous isomerism.The root and rhizome of Salvia miltiorrhiza have been utilized as a reputable traditional Chinese medicine Salviae Miltiorrhizae Radix et Rhizoma(Dan-Shen)in the treatment of cardiovascular disease.Herein,a dimensionenhanced ultra-high performance liquid chromatography/ion mobility/quadrupole time-of-flight mass spectrometry approach in combination with intelligent peak annotation workflows was established aimed to rapidly characterize the multicomponents from S.miltiorrhiza.Due to the sufficient optimization,satisfactory chromatography separation was enabled on an HSS T3 column within 33 min using 0.1%formic acid in water(A)and acetonitrile(B)as the mobile phase,while the data-independent HDMS^(E) in both the negative and positive electrospray ionization modes was utilized for the high-coverage MS^(2) data acquisition.Streamlined automatic peak annotation by searching an in-house library(recording 198 known compounds)followed by the subsequent confirming steps(e.g.,comparison with the reference compounds,fragmentation pathways analysis,and retention behavior comparison,etc.),allowed us to identify or tentatively characterize a total of 86 components(including 50 terpenoids,21 phenolic acids,and 15 others)from S.miltiorrhiza.Importantly,three-dimensional structure information,such as the retention time,MS^(1) and MS^(2) data,and collision cross section(CCS),was provided,which can facilitate the more reliable characterization of herbal components.

关键词： Ultra-high perform liquid chromatography/ion mobility/quadrupole time-of-flight mass spectrometry highdefinition MS^(E) automatic peak annotation Salvia miltiorrhiza phenolic acid tanshinone

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Headspace Solid-Phase Micro-Extraction Gas Chromatography/Mass Spectrometry(HS-SPME-GC/MS)-Based Untargeted Metabolomics Analysis for Comparing the Volatile Components from 12 Panax Herbal Medicines

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Phyton-International Journal of Experimental Botany 2022年第7期91卷 1353-1364页

作者： Simiao wang Xiaohang Li Meiting Jiang Xinlong Wu Yuying Zhao Meiyu Liu Xiaoyan Xu Huimin wang hongda wang Heshui Yu Wenzhi Yang State Key Laboratory of Component-Based Chinese Medicine&Tianjin Key Laboratory of TCM Chemistry and Analysis Tianjin University of Traditional Chinese MedicineTianjin301617China College of Pharmaceutical Engineering of Traditional Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin301617China

Quality control of ginseng currently is mainly based on ginsenoside analysis,but rarely focuses on the volatile organic components.In the current work,an untargeted metabolomics approach,by headspace solid-phase micro-extraction gas chromatography/mass spectrometry(HS-SPME-GC/MS),was elaborated and further employed to holistically compare the compositional difference of the volatile components simultaneously from 12 Panax herbal medicines,which included P.ginseng(PG),P.quinquefolius(PQ),P.notoginseng(PN),red ginseng(PGR),P.ginseng leaf(PGL),P.quinquefolius leaf(PQL),P.notoginseng leaf(PNL),P.ginseng flower(PGF),P.quinquefolius flower(PQF),P.notoginseng flower(PNF),P.japonicus(PJ),and P.japonicus var.major(PJvm).Chromatographic separation was performed on an HP-5MS elastic quartz capillary column using helium as the carrier gas,enabling good resolution within 1 h.We were able to characterize totally 259 volatile compounds,including 82 terpenes(T),46 alcohols(Alc),29 ketones(K),25 aldehydes(Ald),21 esters(E),and the others.By analyzing 90 batches of ginseng samples based on the untargeted metabolomics workflows,236 differential ions were unveiled,and accordingly 36 differential volatile components were discovered.It is the first report that simultaneously compares the compositional difference of volatile components among 12 Panax herbal medicines,and useful information is provided for the quality control of ginseng aside from the well-known ginsenosides.

关键词： Headspace solid-phase micro-extraction gas chromatography/mass spectrometry Panax volatile component untargeted metabolomics

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Super-resolution imaging in glycoscience: New developments and challenges

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Journal of Innovative Optical Health Sciences 2016年第3期9卷 19-34页

作者： Junling Chen Ti Tong hongda wang State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin 130022P.R.China University of Chinese Academy of Sciences Beijing 100049 P.R.China Department of Thoracic Surgery The Second Hospital of Jilin University Changchun Jilin 130041 P.R.China

Carbohydrates on cell surfaces play a crucial role in a wide variety of biological processes,including cell adhesion,recognition and signaling,viral and bacterial infection,in°ammation and metastasis.However,owing to the large diversity and complexity of carbohydrate structure and nongenetically synthesis,glycoscience is the least understood¯eld compared with genomics and proteomics.Although the structures and functions of carbohydrates have been investigated by various conventional analysis methods,the distribution and role of carbohydrates in cell membranes remain elusive.This review focuses on the developments and challenges of super-resolution imaging in glycoscience through introduction of imaging principle and the available°uorescent probes for super-resolution imaging,the labeling strategies of carbohydrates,and the recent applications of super-resolution imaging in glycoscience,which will promote the super-resolution imaging technology as a promising tool to provide new insights into the study of glycoscience.

关键词： Carbohydrate super-resolution imaging

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Interconnected graphene scaffolds for functional gas sensors with tunable sensitivity

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Journal of Materials Science & Technology 2020年第23期58卷 16-23页

作者： Xiao You Jinshan Yang Mengmeng wang hongda wang Le Gao Shaoming Dong State Key Laboratory of High Performance Ceramics&Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing100049China Structural Ceramics and Composites Engineering Research Center Shanghai Institute of CeramicsChinese Academy of SciencesShanghai200050China University of Chinese Academy of Sciences Beijing100039China

In this work,the interconnected graphene scaffolds are prepared by three-dimensional(3 D)printing for multifunctional gas detection with tunable sensitivity.The scaffolds with regularly aligned graphene conductive networks exhibit significant mechanical strength and high electrical stability to multi-direction deformation,which can be attributed to the typical core-shell structure of graphene and PVP.The resistance of the free-standing scaffolds can realize the real-time response to H_(2) O and NO_(2),and the relative resistance change to 100 ppm H_(2) O and 100 ppm NO_(2) can reach 2%and 2.5%,respectively.The charge doping of the oxidizing gases is considered to be the main reason for various response sensitivities of the scaffolds with different orthogonal layers,in which the interconnected conductive network can generate a large specific surface area and significantly improving the adsorption of the target gases and the transfer of charge.The controllable fabrication of regular structure has appropriately great potential for further optimizations and applications in gas detection.

关键词： Graphene scaffolds Interconnected network 3D printing Gas sensors Multifunctional gas detection

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Microstructure and mechanical properties of 3D Cf/SiBCN composites fabricated by polymer infiltration and pyrolysis

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Journal of Advanced Ceramics 2021年第1期10卷 28-38页

作者： Bowen CHEN Qi DING Dewei NI hongda wang Yusheng DING Xiangyu ZHANG Shaoming DONG State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 200050China Structural Ceramics and Composites Engineering Research Center Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 200050China University of Chinese Academy of Sciences Beijing 100049China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

In this work,three-dimensional(3D)Cf/SiBCN composites were fabricated by polymer infiltration and pyrolysis(PIP)with poly(methylvinyl)borosilazane as SiBCN precursor.The 3D microstructure evolution process of the composites was investigated by an advanced X-ray computed tomography(XCT).The effect of dicumyl peroxide(DCP)initiator addition on the crosslinking process,microstructure evolution,and mechanical properties of the composites were uncovered.With the addition of a DCP initiator,the liquid precursor can cross-linking to solid-state at 120℃.Moreover,DCP addition decreases the release of small molecule gas during pyrolysis,leading to an improved ceramic yield 4.67 times higher than that without DCP addition.After 7 PIP cycles,density and open porosity of the final Cf/SiBCN composite with DCP addition are 1.73 g.cm^(-3)and〜10%,respectively,which are 143.0%higher and 30.3%lower compared with the composites without DCP addition.As a result,the flexural strength and elastic modulus of Cf/SiBCN composites with DCP addition(371 MPa and 31 GPa)are 1.74 and 1.60 times higher than that without DCP addition(213 MPa and 19.4 GPa),respectively.

关键词： Cf/SiBCN ceramic matrix composites dicumyl peroxide(DCP) X-ray computed tomography(XCT)

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Offline two-dimensional liquid chromatography coupled with ion mobility-quadrupole time-of-flight mass spectrometry enabling fourdimensional separation and characterization of the multicomponents from white ginseng and red ginseng

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Journal of Pharmaceutical Analysis 2020年第6期10卷 597-609页

作者： Tiantian Zuo Chunxia Zhang Weiwei Li hongda wang Ying Hu Wenzhi Yang Li Jia Xiaoyan wang Xiumei Gao Dean Guo Tianjin State Key Laboratory of Modern Chinese Medicine Tianjin University of Traditional Chinese Medicine312 Anshanxi RoadTianjin300193China Tianjin Key Laboratory of TCM Chemistry and Analysis Tianjin University of Traditional Chinese Medicine312 Anshanxi RoadTianjin300193China Shanghai Research Center for Modernization of Traditional Chinese Medicine National Engineering Laboratory for TCM Standardization TechnologyShanghai Institute of Materia MedicaChinese Academy of Sciences501 Haike RoadShanghai201203China

Inherent complexity of plant metabolites necessitates the use of multi-dimensional information to accomplish comprehensive profiling and confirmative identification.A dimension-enhanced strategy,by offline two-dimensional liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry(2 D-LC/IM-QTOF-MS)enabling four-dimensional separations(2 D-LC,IM,and MS),is proposed.In combination with in-house database-driven automated peak annotation,this strategy was utilized to characterize ginsenosides simultaneously from white ginseng(WG)and red ginseng(RG).An offline 2 DLC system configuring an Xbridge Amide column and an HSS T3 column showed orthogonality 0.76 in the resolution of ginsenosides.Ginsenoside analysis was performed by data-independent high-definition MSE(HDMSE)in the negative ESI mode on a Vion?IMS-QTOF hybrid high-resolution mass spectrometer,which could better resolve ginsenosides than MSEand directly give the CCS information.An in-house ginsenoside database recording 504 known ginsenosides and 58 reference compounds,was established to assist the identification of ginsenosides.Streamlined workflows,by applying UNIFI?to automatedly annotate the HDMSEdata,were proposed.We could separate and characterize 323 ginsenosides(including 286 from WG and 306 from RG),and 125 thereof may have not been isolated from the Panax genus.The established 2 D-LC/IM-QTOF-HDMSEapproach could also act as a magnifier to probe differentiated components between WG and RG.Compared with conventional approaches,this dimensionenhanced strategy could better resolve coeluting herbal components and more efficiently,more reliably identify the multicomponents,which,we believe,offers more possibilities for the systematic exposure and confirmative identification of plant metabolites.

关键词： Dimension-enhanced strategy Multicomponent characterization Ginsenoside Offline two-dimensional liquid chromatography Ion mobility-quadrupole time-of-flight mass spectrometry In-house database

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