检索结果-南通市图书馆

Advances in oral mesenchymal stem cell-derived extracellular vesicles in health and disease

Genes & Diseases 2024年第1期11卷 346-357页

作者： Huanyu Luo Anahid Ahmadi Birjandi Feilong Ren Tianmeng sun Paul T.Sharpe hongchen sun Zhengwen An Department of Oral Biology Jilin Provincial Key Laboratory of Tooth Development and Bone RemodelingSchool and Hospital of StomatologyJilin UniversityChangchunJilin 130021China Faculty of Dentistry Oral&Craniofacial SciencesCentre for Craniofacial and Regenerative BiologyKing's College LondonLondonSE19RTUK Department of Oral Pathology Jilin Provincial Key Laboratory of Tooth Development and Bone RemodelingSchool and Hospital of StomatologyJilin UniversityChangchunJilin 130021China

Extracellular vesicles(EVs)are nano-size vesicles secreted naturally by all cells into the extracellular space and have been recognized as important cell-cell mediators in multicel-lular organisms.EVs contain nucleic acids,proteins,lipids,and other cellular components,regulating many basic biological processes and playing an important role in regenerative med-icine and diseases.EVs can be traced to their cells of origin and exhibit a similar function.Moreover,EVs demonstrate low immunogenicity,good biocompatibility,and fewer side ef-fects,compared to their parent cells.Mesenchymal stem cells(MsCs)are one of the most important resource cells for EVs,with a great capacity for self-renewal and multipotent differ-entiation,and play an essential role in stem cell therapy.The mechanism of MsC therapy was thought to be attributed to the differentiation of MsCs after targeted migration,as previously noted.However,emerging evidence shows the previously unknown role of MsC-derived para-crine factors in stem cell therapy.Especially EVs derived from oral tissue MSCs(OMSC-EVs),show more advantages than those of all other MsCs in tissue repair and regeneration,due to their lower invasiveness and easier accessibility for sample collection.Here,we systematically review the biogenesis and biological characteristics of OMSC-EVs,as well as the role of OMsC-EVs in intercellular communication.Furthermore,we discuss the potential therapeutic roles of OMSC-EVs in oral and systemic diseases.We highlight the current challenges and future directions of OMSC-EVs to focus more attention on clinical translation.We aim to provide valuable insights for the explorative clinical application of OMSC-EVs.

关键词： Disease Extracellular vesicles Oral tissue MSC Therapeutic application Tissue regeneration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Immunomodulatory effects and mechanisms of distraction osteogenesis

引用

International Journal of Oral Science 2022年第1期14卷 20-30页

作者： Shude Yang Ning Wang Yutong Ma Shuaichen Guo Shu Guo hongchen sun Department of Plastic Surgery The First Hospital of China Medical UniversityShenyangChina Liaoning Provincial Key Laboratory of Oral Diseases School of Stomatology and Department of Oral PathologySchool of StomatologyChina Medical UniversityShenyangChina Department of Breast Surgery The First Hospital of China Medical UniversityShenyangChina China Medical University ShenyangChina

Distraction osteogenesis(DO) is widely used for bone tissue engineering technology. Immune regulations play important roles in the process of DO like other bone regeneration mechanisms. Compared with others, the immune regulation processes of DO have their distinct features. In this review, we summarized the immune-related events including changes in and effects of immune cells, immune-related cytokines, and signaling pathways at different periods in the process of DO. We aim to elucidated our understanding and unknowns about the immunomodulatory role of DO. The goal of this is to use the known knowledge to further modify existing methods of DO, and to develop novel DO strategies in our unknown areas through more detailed studies of the work we have done.

关键词： osteogenesis Immune summarized

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Rational design of hydrogels for immunomodulation

引用

Regenerative Biomaterials 2022年第1期9卷 979-1000页

作者： Wenhuan Bu Yuanhao Wu Amir M.Ghaemmaghami hongchen sun Alvaro Mata Liaoning Provincial Key Laboratory of Oral Diseases School of StomatologyChina Medical UniversityNo.117Nanjing North StreetHeping DistrictShenyang 110001China Department of Dental Materials School of StomatologyChina Medical UniversityNo.117Nanjing North StreetHeping DistrictShenyang 110001China Department of Center Laboratory School of StomatologyChina Medical UniversityNo.117Nanjing North StreetHeping DistrictShenyang 110001China School of Pharmacy University of NottinghamUniversity ParkNottingham NG72RDUK Biodiscovery Institute University of NottinghamUniversity ParkNottingham NG72RDUK Division of Immunology School of Life SciencesUniversity of NottinghamUniversity ParkNottingham NG72RDUK Terasaki Institute for Biomedical Innovation 1018 Westwood BlvdLos AngelesCA 90024USA Department of Chemical and Environmental Engineering University of NottinghamUniversity ParkNottingham NG72RDUK

The immune system protects organisms against endogenous and exogenous harm and plays a key role in tissue development,repair and regeneration.Traditional immunomodulatory biologics exhibit limitations including degradation by enzymes,short half-life and lack of targeting ability.Encapsulating or binding these biologics within biomaterials is an effective way to address these problems.Hydrogels are promising immunomodulatory materials because of their prominent biocompatibility,tuneability and versatility.However,to take advantage of these opportunities and optimize material performance,it is important to more specifically elucidate,and leverage on,how hydrogels affect and control the immune response.Here,we summarize how key physical and chemical properties of hydrogels affect the immune response.We first provide an overview of underlying steps of the host immune response upon exposure to biomaterials.Then,we discuss recent advances in immunomodulatory strategies where hydrogels play a key role through(i)physical properties including dimensionality,stiffness,porosity and topography;(ii)chemical properties including wettability,electric property and molecular presentation;and(iii)the delivery of bioactive molecules via chemical or physical cues.Thus,this review aims to build a conceptual and practical toolkit for the design of immune-instructive hydrogels capable of modulating the host immune response.

关键词： hydrogels immunomodulation physical properties chemical properties

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Brasenia-inspired hydrogel with sustained and sequential release of BMP and WNT activators for improved bone regeneration

引用

Chinese Chemical Letters 2023年第7期34卷 162-168页

作者： Xinqing Hao Xuewei Zhang Yue Hu Chunxia Ren Cangwei Liu Lu Wang Yijun Zhou Shuangshuang Wang Huanyu Luo Guangxing Yan Xiao Wang Xiaomeng Wang Feilong Ren Ce Shi, Wenlong Song hongchen sun Hospital of Stomatology Jilin UniversityChangchun 130021China School of Materials Science and Engineering Hainan UniversityHaikou 570228China State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchun 130012.China School of Stomatology China Medical UniversityShenyang 110001China jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling Changchun 130021China

Although bone morphogenetic protein(BMP) and WNT signaling play pivotal roles in bone development,homeostasis, and regeneration, the applications of proteins to stimulate corresponding signaling pathways showed limited outcomes in the repair and regeneration of bone defects that might be attributed to the reciprocal interventions of these pathways. In order to satisfy the combinational and sequential activation of BMP and WNT pathways, inspired by the heterogeneous hydrogel-liked structures of Brasenia, heterogeneous alginate/chitosan hydrogels were fabricated and spatially loaded with FK506 and BIO to achieve sustained and sequential release of the activators. Alkaline phosphatase staining, alizarin red staining and q RT-PCR results suggested that FK506 and BIO enhanced osteoblastic differentiation in vitro when used separately. Besides, by mixing and matching the activators and the hydrogel layers, a superior releasing mode that a combination of early FK506 release and following BIO release was identified via both in vitro and in vivo explorations for most efficient bone regeneration. These results suggested that drug-loaded heterogeneous hydrogels possess great potentials in treating bone loss defects for future clinical practice.

关键词： Bone regeneration FK506 BIO Heterogeneous hydrogels Osteogenic differentiation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Polycation-functionalized gold nanodots with tunable near-infrared fluorescence for simultaneous gene delivery and cell imaging

引用

Nano Research 2018年第5期11卷 2392-2404页

作者： Yuanqing sun Dandan Wang Yueqi Zhao Tianxin Zhao hongchen sun Xiangwei Li Chuanxi Wang Bai Yang Quan Lin State Key Laboratory of Supramolecular Structure and Materials College of Chemistry Jilin University Changchun 130012 China Institute of New Energy Technology Ningbo Institute of Industrial Technology Chinese Academy of Sciences Ningbo 315201 China School ofStomatology Jilin University Changchun 130041 China State Key Laboratory of Heavy Oil Processing College of Science China University of Petroleum Beijing 102249 China

Near-infrared （NIR） fluorescent metal nanodots may have significant advantages in biological detection and bioimaging. Herein, we introduce tunable near-infrared fluorescent gold nanodots （AuNDs） protected by branched polyethylenimine （PEI） modified by surface segmental attachment of sulfhydryl groups （PEI-SH）, abbreviated as PEI-SH-AuNDs, for simultaneous gene delivery and cell imaging. The modified PEI endows the resultant PEI-SH-AuNDs with the following excellent advantages. Sulfhydryl groups of PEI-SH anchor to the surface of AuNDs, and such polycations with amine groups give PEI-SH-AuNDs remarkable stability. The cationic polymer PEI-SH with positive charges enables PEI-SH-AuNDs to perform gene delivery, and the gene transfection efficiency can reach 22.8%. Moreover, the fluorescence of PEI-SH-AuNDs is tunable from visible red light （wavelength 609 nm） to NIR light （wavelength 811 run） via an increase in the size of AuNDs. PEI-SH-AuNDs yielded gene transfection efficiency similar to that of commercial PEI, but showed much lower cytotoxicity and much greater red-shift fluorescence. With excellent photoluminescent properties, such multifunctional fluorescent PEI-SH-AuNDs hold promise in applications to bioimaging and as ideal fluorescent probes for tracking gene transfection behavior.

关键词： near-infrared fluorescent material,Au nanodot,tunable fluorescence,gene deliver,bioimaging

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Regulation of FN1 degradation by the p62/SQSTM1-dependent autophagy-lysosome pathway in HNSCC

引用

International Journal of Oral Science 2020年第4期12卷 327-337页

作者： Xinchen Liu Lin Meng Xing Li Daowei Li Qilin Liu Yumeng Chen Xiangwei Li Wenhuan Bu hongchen sun Department of Oral Biology Jilin Provincial Key Laboratory of Tooth Development and Bone RemodelingSchool and Hospital of StomatologyJilin UniversityChangchunChina Department of Endodontics School and Hospital of StomatologyJilin UniversityChangchunChina Department of Pathology School and Hospital of StomatologyJilin UniversityChangchunChina School and Hospital of Stomatology China Medical UniversityShenyangChina

Epithelial–mesenchymal transition(EMT)is involved in both physiological and pathological processes.EMT plays an essential role in the invasion,migration and metastasis of tumours.Autophagy has been shown to regulate EMT in a variety of cancers but not in head and neck squamous cell carcinoma(HNSCC).Herein,we investigated whether autophagy also regulates EMT in HNSCC.Analyses of clinical data from three public databases revealed that higher expression of fibronectin-1(FN1)correlated with poorer prognosis and higher tumour pathological grade in HNSCC.Data from SCC-25 cells demonstrated that rapamycin and Earle’s balanced salt solution(EBSS)promoted autophagy,leading to increased FN1 degradation,while 3-methyladenine(3-MA),bafilomycin A1(Baf A1)and chloroquine(CQ)inhibited autophagy,leading to decreased FN1 degradation.On the other hand,autophagic flux was blocked in BECN1 mutant HNSCC Cal-27 cells,and rapamycin did not promote autophagy in Cal-27 cells;also in addition,FN1 degradation was inhibited.Further,we identified FN1 degradation through the lysosome-dependent degradation pathway using the proteasome inhibitor MG132.Data from immunoprecipitation assays also showed that p62/SQSTM1 participated as an autophagy adapter in the autophagy–lysosome pathway of FN1 degradation.Finally,data from immunoprecipitation assays demonstrated that the interaction between p62 and FN1 was abolished in p62 mutant MCF-7 and A2780 cell lines.These results indicate that autophagy significantly promotes the degradation of FN1.Collectively,our findings clearly suggest that FN1,as a marker of EMT,has adverse effects on HNSCC and elucidate the autophagy–lysosome degradation mechanism of FN1.

关键词： inhibited invasion FN1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于深度学习的无人机检测和识别研究综述

引用

信号处理 2024年第4期40卷 609-624页

作者：那振宇程留洋孙鸿晨林彬大连海事大学信息科学技术学院辽宁大连116026 广东省空天通信与网络技术重点实验室广东深圳518055

近年来,由于在各行各业发挥了不可替代作用,无人机产业和应用得到了迅速发展。然而,无人机的“黑飞”、携带危险物品等事件频繁发生,对社会安全构成了严重威胁。因此,无人机的检测和识别变得尤为迫切和必要。随着无人机类型不断地变化,... 详细信息

近年来,由于在各行各业发挥了不可替代作用,无人机产业和应用得到了迅速发展。然而,无人机的“黑飞”、携带危险物品等事件频繁发生,对社会安全构成了严重威胁。因此,无人机的检测和识别变得尤为迫切和必要。随着无人机类型不断地变化,传统的检测与识别方法已不再适应当前需求。深度学习技术的快速发展为无人机检测与识别提供了一种高效且准确的解决方案。深度学习模型具备自主学习特征的能力,能够从大规模数据中提取高级特征,并且在无人机检测与识别任务中表现出色。该模型不仅能够显著提高准确性,还能够适应各种复杂环境和无人机类型。对此,本文综述了基于深度学习的无人机检测与识别技术的最新进展,主要包括基于深度学习的无人机视觉检测和识别、基于深度学习的无人机音频检测和识别、基于深度学习的无人机雷达检测和识别以及基于深度学习的无人机射频检测和识别。最后,对目前无人机检测和识别现存问题进行分析,并展望了未来研究方向。

关键词：无人机检测和识别深度学习射频

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Construction of hollow polydopamine nanoparticle based drug sustainable release system and its application in bone regeneration

引用

International Journal of Oral Science 2021年第3期13卷 282-291页

作者： Lu Wang Shuwei Liu Chunxia Ren Siyuan Xiang Daowei Li Xinqing Hao Shilei Ni Yixin Chen Kai Zhang hongchen sun Department of Oral Pathology Hospital of StomatologyJilin UniversityChangchunChina Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling School and Hospital of StomatologyJilin UniversityChangchunChina State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchunChina

Nanomaterial-based drug sustainable release systems have been tentatively applied to bone regeneration.They,however,still face disadvantages of high toxicity,low biocompatibility,and low drug-load capacity.In view of the low toxicity and high biocompatibility of polymer nanomaterials and the excellent load capacity of hollow nanomaterials with high specific surface area,we evaluated the hollow polydopamine nanoparticles(HPDA NPs),in order to find an optimal system to effectively deliver the osteogenic drugs to improve treatment of bone defect.Data demonstrated that the HPDA NPs synthesized herein could efficiently load four types of osteogenic drugs and the drugs can effectively release from the HPDA NPs for a relatively longer time in vitro and in vivo with low toxicity and high biocompatibility.Results of qRT-PCR,ALP,and alizarin red S staining showed that drugs released from the HPDA NPs could promote osteogenic differentiation and proliferation of rat bone marrow mesenchymal stem cells(rBMSCs)in vitro.Image data from micro-CT and H&E staining showed that all four osteogenic drugs released from the HPDA NPs effectively promoted bone regeneration in the defect of tooth extraction fossa in vivo,especially tacrolimus.These results suggest that the HPDA NPs,the biodegradable hollow polymer nanoparticles with high drug load rate and sustainable release ability,have good prospect to treat the bone defect in future clinical practice.

关键词： drugs dopamine release

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Resistive Switching Properties and Failure Behaviors of(Pt, Cu)/Amorphous ZrO_(2)/Pt Sandwich Structures

引用

Journal of Materials Science & Technology 2016年第7期32卷 676-680页

作者： Haifa Zhai Jizhou Kong Jien Yang Jing Xu Qingran Xu hongchen sun Aidong Li Di Wu Henan Province Key Laboratory of Photovoltaic Materials College of Physics and Electronic Engineering Henan Normal University National Laboratory of Solid State Microstructures Department of Materials Science and Engineering College of Engineering and Applied SciencesNanjing University State Key Laboratory of Mechanics and Control of Mechanical Structures Nanjing University of Aeronautics and Astronautics

The effect of Pt and Cu electrodes on the resistive switching properties and failure behaviors of amor- phous ZrO2 films were investigated. Compared with Cu/ZrO2/Pt structures, the Pt/ZrO2/Pt structures exhibit better resistive switching properties such as the higher resistance ratio of OFF/ON states, the longer switch- ing cycles and narrow distribution of OFF state resistance （Roff）. The switching mechanism in the Pt/ZrO2/ Pt structure can be attributed to the formation and rupture of oxygen vacancy filaments; while in the Cu/ZrO2/Pt structure, there exist both oxygen vacancy filaments and Cu filaments. The formation of Cu filaments is related to the redox reaction of Cu electrode under the applied voltage. The inhomoge- neous dispersive injection of Cu ions results in the dispersive Ro~ and significant decrease of operate voltage, Schematic diagrams of the formation of conductive filaments and the failure mechanism in the Cu/ZrO2/ Pt structures are also proposed.

关键词： ZrO2 Thin films Sol-gel method Failure

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ultra-small nanodots coated with oligopeptides providing highly negative charges to enhance osteogenic differentiation of hBMSCs better than osteogenic induction medium

引用

Chinese Chemical Letters 2021年第1期32卷 266-270页

作者： Yueqi Zhao Lin Meng Kai Zhang Yuanqing sun Yue Zhao Zhe Yang Yangliu Lin Xinrui Liu hongchen sun Bai Yang Quan Lin State Key Laboratory of Supramolecular Structure and Material College of ChemistryJilin UniversityChangchun 130012China School and Hospital of Stomatology China Medical UniversityShenyang 110002China State Key Laboratory of Heavy Oil Processing College of ScienceChina University of PetroleumBeijing 102249China School and Hospital of Stomatology Jilin UniversityChangchun 130021China Department of Neurosurgical Oncology The First Hospital of Jilin UniversityChangchun 130021China

For bone regenerative engineering,it is a promising method to form skeletal tissues differentiating from human bone morrow mesenchyme stem cells(hBMSCs).However,it is still a critical challenge to efficiently control ostogenesis and clearly reveal the influence factor.To this end,the fluorescent gold nanodots(Au NDs) with highly negative charges as osteogenic induction reagent are successfully synthesized,which display better than commercial osteogenic induction medium through the investigations of ALP activity(2.5 folds) and cytoskeleton staining(1.5 folds).Two kinds of oligopeptides with different bio-structures(cysteine,Cys and glutathione,GSH) are selected for providing surficial charges on Au NDs.It is revealed that Au-Cys with more negative charges(-51 mV) play better role than Au-GSH(-19 mV) in osteogenic differentiation,when both of them have same size(~2 nm),sphere shape and show similar cell uptake amount.To explore deeply,osteogenesis related signaling pathways are monitored,revealing that the enhancement of osteogenic differentiation was through autophagy signaling pathway triggered by Au-Cys.And the promotion of highly negative charges in osteogenic diffe rentiation was further proved via sliver nanodots(Ag NDs,Ag-Cys and Ag-GSH) and carbon nanodots(CDs,Cys-CDs and GSH-CDs).This work indicates part of insights during hBMSCs differentiation and provides a novel strategy in osteogenic differentiation process.

关键词： Osteogenic differentiation Negative charges from oligopeptide Sub-3 nm fluorescent Au nanodots Better than osteogenic induction medium Autophagy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：