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VISUALIZATION OF HEAD AND NECK CANCER MODELS WITH A TRIPLE FUSION REPORTER GENE

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Journal of Innovative Optical Health Sciences 2012年第4期5卷 48-56页

作者： YING ZHENG QIAOYA LIN honglin jin JUAN CHEN ZHIHONG ZHANG Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology Wuhan 430074P.R.China MoE Key Laboratory for Biomedical Photonics Department of Biomedical Engineering Huazhong University of Science and Technology Wuhan 430074 P.R.China Campbell Family Cancer Research Institute and Ontario Cancer Institute University Health Network TorontoP.R.Canada

The development of experimental animal models for head and neck tumors generally rely on the biol uminescence imaging to achieve the dynamic monitoring of the tumor growth and metastasis due to the complicated anatomical *** the bioluminescence imaging is largely affected by the intracellular luciferase expression level and external D-luciferin concentrations,its imaging accuracy requires further ***,a new triple fusion reportelr gene,which consists of a herpes simplex virus type 1 thymidine kinase(TK)gene for radioactive imaging,a far-red fuorescent protein(mLumin)gene for fuorescent imaging,and a firefly luciferase gene for bioluminescence imaging,was introduced for in vrivo observation of the head and neck tumors through multi-modality *** show that fuorescence and bioluminescence signals from mLumin and luciferase,respectively,were clearly observed in tumor cells,and TK could activate suicide pathway of the cells in the presence of nucleotide analog-ganciclovir(GCV),demonstrating the effecti veness of individual functions of each ***,subcutaneous and metastasis animal models for head and neck tumors using the fusion reporter gene-expressing cell lines were established,allowing multi-modality imaging in ***,the established tumor models of head and neck cancer based on the newly developed triple fusion reporter gene are ideal for monitoring tumor growth,assessing the drug therapeutic efficacy and verifying the effec-tiveness of new treatments.

关键词： Head and neck cancer tumor metastasis model three fusion reporter gene far-red fluorescent protein frefly luciferase multi-modality imaging

KillerRed protein based in vivo photodynamic therapy and corresponding tumor metabolic imaging

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Journal of Innovative Optical Health Sciences 2016年第1期9卷 21-30页

作者： Qiaoya Lin Shuang Sha Fei Yang honglin jin Zhihong Zhang Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 P.R.China MoE Key Laboratory for Biomedical Photonics Department of Biomedical Engineering Huazhong University of Science and Technology Wuhan 430074 P.R.China

Photodynamic therapy(PDT)gains wide attention as a useful therapeutic method for *** is mediated by the oxygen and photosensitizer under the specific light irradiation to produce the reactive oxygen species(ROS),which induce cellular toxicity and regulate the redox potential in tumor ***,genetic photosensitizers of low toxicity and easy production are required to be ***,a unique red fluorescent protein exhibiting excellent phototoxic properties,has the potential to act as a photosensitizer in the application of tumor ***,the course of tumor redox metabolism during this treatment was rarely investigated so *** here,we investigated the effects of KillerRed-based PDT on tumor growth in vivo and examined the subsequent tumor metabolic states including the changes of nicotinamide adenine dinucleotide hydrogen(NADH)and flavoprotein(Fp),two important metabolic coenzymes of tumor *** showed the tumor growth had been significantly inhibited by KillerRedbased PDT treatment compared to control groups.A home-made cryo-imaging redox scanner was used to measure intrinsic fluorescence and exogenous KillerRed fluorescence signals in *** Fp signal was elevated by nearly 4.5-fold,while the NADH signal decreased by 66%after light irradiation,indicating that Fp and NADH were oxidized in the course of KillerRedbased ***,we also observed correlation between the fluorescence distribution of KillerRed and *** suggests that the KillerRed protein based PDT might provide a new approach for tumor therapy accompanied by altering tumor metabolism.

关键词： Reactive oxygen species redox metabolism flavoprotein nicotinamide adenine dinucleotide hydrogen

Upper crustal deformation characteristics in the northeastern Tibetan Plateau and its adjacent areas revealed by GNSS and anisotropy data

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Earthquake Science 2023年第4期36卷 297-308页

作者： Shuyu Li Yuan Gao honglin jin Key Laboratory of Earthquake Prediction Institute of Earthquake ForecastingChina Earthquake AdministrationBeijing 100036China

The northeastern part of the Tibetan Plateau is a region where different tectonic blocks collide and intersect,and large earthquakes are *** Navigation Satellite System(GNSS)observations show that tectonic deformation in this region is strong and manifests as non-uniform deformation associated with tectonic features.S-wave splitting studies of near-field seismic data show that seismic anisotropy parameters can also reveal the upper crustal medium deformation beneath the reporting *** this paper,we summarize the surface deformation from GNSS observations and crustal deformation from seismic anisotropy data in the northeastern Tibetan *** comparing the principal compressive strain direction with the fast S-wave polarization direction of near-field S-wave splitting,we analyzed deformation and its differences in surface and upper crustal media in the northeastern Tibetan Plateau and adjacent *** principal compressive strain direction derived from GNSS is generally consistent with the polarization direction of fast S-waves,but there are also local tectonic regions with large differences between them,which reflect the different deformation mechanisms of regional upper crustal *** combination of GNSS and seismic anisotropy data can reveal the depth variation characteristics of crustal deformation and deepen understanding of three-dimensional crustal deformation and the deep dynamical mechanisms underlying ***.

关键词： upper crustal deformation northeastern Tibetan Plateau GNSS seismic anisotropy deformation differences

Comparison of caspase-3 activation in tumor cells upon treatment of chemotherapeutic drugs using capillary electrophoresis

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Protein & Cell 2012年第5期3卷 392-399页

作者： Shuang Sha honglin jin Xiao Li Jie Yang Ruiting Ai jinling Lu Britton Chance Center for Biomedical Photonics-Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074China Key Laboratory of Biomedical Photonics of Ministry of Education Huazhong University of Science and TechnologyWuhan 430074China China National Center for Biotechnology Development Beijing 100036China

Caspases play important roles in cell ***-urement of the dynamics of caspase activation in tumor cells not only facilitates understanding of the molecular mechanisms of apoptosis but also contributes to the development,screening,and evaluation of anticancer drugs that target apoptotic *** fluorescence resonance energy transfer(FRET)technique provides a valuable approach for defining the dynamics of apop-tosis with high spatio-temporal ***,FRET generally functions in the single-cell level and becomes ineffective when applied in the high throughput detection of caspase *** the cur-rent study,a FRET sensor was combined with capillary electrophoresis(CE)to achieve a high throughput method for cellular caspase *** FRET-based CE system is composed of a homemade CE system and a laser source for detecting the dynamics of caspase-3 in various cells expressing sensors of caspase-3 that have been treated with anticancer drugs,such as cell cycle-independent drug cisplatin and specific cell cycle drugs camptothecin and etoposide,as well as their combination with tumor necrosis factor(TNF).A posi-tive correlation between the caspase-3 activation ve-locity and drug concentration was observed when the cells were treated with cisplatin,but cells induced by camptothecin and etoposide did not show any apparent correlation with their ***,differ-ent types of cells presented distinct sensitivities under the same drug treatment,and the combination treat-ment of TNF and anticancer drugs significantly accel-erated the caspase-3 activation *** high throughput capability and detection sensitivity make the FRET-based CE system a useful tool for investi-gating the mechanisms of anticancer drugs and anti-cancer drug screening.

关键词： apoptosis caspase-3 fluorescence reso-nance energy transfer(FRET) capillary electrophoresis(CE)

Fluorescent and quantitative mitochondrial redox imaging of tumor targeted by Octa-RGD probe

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Journal of Innovative Optical Health Sciences 2016年第4期9卷 55-65页

作者： Shuang Sha Fei Yang Anle Wang honglin jin Zhihong Zhang Qiaoya Lin Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics–Huazhong University of Science and Technology Wuhan 430074 P.R.China MoE Key Laboratory for Biomedical Photonics Department of Biomedical Engineering Huazhong University of Science and Technology Wuhan 430074 P.R.China

Integrins,over-expressed in a broad range of cancer diseauses,are widely utilized as a tumor *** investigation also plays important roles in tumor ***-oping simple integrin-targetting probe and monitoring tumor metabolism will give opportunities to find ways for cancer treatment,however,the investigation of tumor metabolism with integrin receptor based probes has been rarely reported so ***,we developed an octavalent fuo-rescent probe *** by convenient genetic method,based on one tetrameric far-red fluo-rescent protein(fRFP)linked with RGD pept *** validated its inter gin targeting by confocal imaging in *** we screened a variety of tumor cells,and differentiated their binding affinity based on the fuorescence of the probe via flow *** these cells,CNE-2 cells had the highest uptake of the probe,while B16 cells had the lowest,corresponding with their intergin expression ***,the fuorescent and metabolic imaging was performed in HT1080(intergin postive)tumor,where nicotinamide adenine dinudeotide hydrogen(NADH),flavo-protein(Fp)and fRFP fuorescent signals were *** tumor from mice intravenously injected with Octa RGD probe displayed obviously higher NADH redox ratio NADH/(Fp+NADH)and fRFP signal,than those with fRFP *** suggested that integrin targeting may have influence on the target cell metabolism,and further demonstrated *** probe facilitated its uptake in the targeted tumor in *** paper developed a useful probe,which can bind integrins speci-cally and e±ciently in tumor cells,and together with tumor metabolic information,it may provide new insight for RGD targeting-based cancer therapeutics.

关键词： Integrin redox metabolism fRFP NADH Fp.

Contemporary crustal tectonic movement in the southern Sichuan-Yunnan block based on dense GPS observation data

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Earth and Planetary Physics 2019年第1期3卷 53-61页

作者： honglin jin Yuan Gao XiaoNing Su GuangYu Fu Key Laboratory of Earthquake Prediction China Earthquake Administration Institute of Earthquake Forecasting China Earthquake Administration

We analyzed 360 permanent and campaign GPS data from 1999 to 2017 in the southern Sichuan-Yunan block, and obtained crustal horizontal deformation in this ***, we derived the strain rate using a multi-scale spherical wavelet *** reveal a complex pattern of tectonic movement in the southern Sichuan-Yunnan *** to the stable Eurasian plate, the maximum rate of the horizontal deformation in the southern Sichuan-Yunnan block is approximately 22 mm/*** Xiaojiang fault shows a significantly lower deformation—a left-lateral strike-slip movement of 9.5 mm/*** Honghe fault clearly shows a complex segmental deformation from the north to *** northern Honghe fault shows 4.3 mm/a right strike-slip with 6.7 mm/a extension; the southern Honghe fault shows 1.9 mm/a right strike-slip with 1.9 mm/a extension; the junction zone in the Honghe and Lijiang–Xiaojinhe faults shows an obvious clockwise-rotation *** strain calculation results reveal that the maximum shear-strain rate in this region reaches 70 nstrain/a, concentrated around the Xiaojiang fault and at the junction of the Honghe and Lijiang–Xiaojinhe *** note that most of the earthquakes with magnitudes of 4 and above that occurred in this region were within the high shear strain-rate zones and the strain rate gradient boundary zone, which indicates that the magnitude of strain accumulation is closely related to the seismic *** of the fast shear-wave polarization direction of the upper-crust with the upper-mantle anisotropy and the direction of the surface principal compressive strain rate obtained from the inversion of the GPS data reveals that the direction of the surface principal compressive strain is basically consistent with the fast shear-wave polarization direction of the upper crust anisotropy, but different from the polarization direction of the upper *** results support the hypothesis that the principal elements of the deformation mecha

关键词： GPS data crustal horizontal deformation extension strike slip strain rate fast shear-wave polarization

Role of nanoparticle-mediated immunogenic cell death in cancer immunotherapy

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Asian Journal of Pharmaceutical Sciences 2021年第2期16卷 129-132页

作者： Yajie Sun Xiangru Feng Chao Wan Jonathan F.Lovell honglin jin Jianxun Ding Union Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China China Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China Department of Biomedical Engineering University at BuffaloState University of New York.BuffaloNew York 14260USA

An essential concept of cancer immunotherapy is that immunogenic cell death(ICD),characterized by the release of tumor-associated antigens(TAAs)and tumor-specific antigens(TSAs)like neoantigens,danger-associated molecular patterns(DAMPs),and pro-inflammatory cytokines,facilitates the presentation of TAAs and TSAs to adaptive immune cells,eliciting an emerging or reinstating a pre-existing anti-cancer immune response.

关键词： immunotherapy cancer death

Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy

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Bioactive Materials 2022年第3期7卷 541-553页

作者： Yuhan Zhou Ting Ye Chengzhi Ye Chao Wan Siyue Yuan Yushuai Liu Tianyu Li Fagang Jiang Jonathan F.Lovell honglin jin jing Chen Cancer Center Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022PR China Department of Pediatrics Renmin Hospital of Wuhan UniversityWuhan430060PR China Department of Ophthalmology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022PR China Department of Biomedical Engineering University at BuffaloState University of New York.BuffaloNew York14260USA College of Biomedicine and Health and College of Life Science and Technology Huazhong Agricultural UniversityWuhan430070China

Autologous tumor cells and cell-derived secretions(CDS)can induce antitumor immune *** conditions in which cells are cultured and treated impact CDS,and cellular insults alter their composition and *** this study,we generated CDS from tumor cells exposed to normal culture conditions,hypoxia,cisplatin,radiotherapy,photodynamic therapy,or hypochlorous acid(HOCl).In vitro HOCl-CDS showed the strongest stimulatory effects on dendritic cells and macrophages compared to CDS generated by hypoxia,cisplatin,radiotherapy or photodynamic *** improve HOCl-CDS activity at the tumor site,we loaded HOCl-CDS into a melittin-encapsulated hydrogel *** injected intratumorally,the HOCl-CDS hydrogel promoted tumor cell death,cytotoxic T lymphocyte infiltration,and tumor-associated macrophage reprogramming towards an M1 *** hydrogel inhibited tumor growth and prolonged the survival of mice bearing B16-F10 ***,hydrogel-delivered HOCl-CDS augmented the antitumor effects of immune checkpoint *** results underscore the importance of the CDS generation method and delivery approach for improving cancer immunotherapy.

关键词： Cancer immunotherapy Hydrogel Melittin Anti-PD-1 Cell-derived secretions

Slow fault activity and micro-bock motion in North China: a perspective from GPS measurements

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Slow fault activity and micro-bock motion in North China: a ...