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Effects of acupuncture and moxibustion on chromatin remodeling-related enzymes in the colon tissue of rats with Crohn disease

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Journal of Acupuncture and Tuina Science 2024年第5期22卷 353-362页

作者： LI Yanting GUO Yajing ZHAO Jimeng SUN Yi GUO Sen shi Yin Seventh People’s Hospital Affiliated to Shanghai University of Traditional Chinese Medicine Shanghai 200137China Shanghai University of Traditional Chinese Medicine Shanghai 201203China Shanghai Research Institute of Acupuncture and Meridian Shanghai 200030China

Objective:To investigate the regulatory effects of acupuncture and moxibustion on chromatin remodeling complex core catalytic subunit of Brahma-related gene 1(Brg1),histone deacetylase(HDAC)3,HDAC9,and males absent on the first(MOF)in the colon tissue of rats with Crohn disease(CD).Methods:Using the random number table method,60 male Sprague-Dawley rats were divided into 5 groups,including a normal group,a model group,an acupuncture group,a medicinal cake-insulated moxibustion group,and an acupuncture-moxibustion group,with 12 rats in each *** rat models were prepared using 2,4,6-trinitrobenzene sulfonic acid(TNBS)in all groups except the normal *** normal and model groups received no *** the acupuncture group,rats were intervened with acupuncture at bilateral Zusanli(ST36)and Shangjuxu(ST37),20 min/session,once a *** medicinal cake-insulated moxibustion group received medicinal cake-insulated moxibustion at Qihai(CV6)and bilateral Tianshu(ST25)with 2 cones per point per session,once a *** acupuncture-moxibustion group received both acupuncture and moxibustion interventions *** intervention was performed for 10 consecutive *** included general condition,disease activity,macroscopic damage,and pathological changes in the rat’s colon *** fluorescence quantitative polymerase chain reaction was used to measure the mRNA expression of chromatin remodeling-related enzymes Brg1,HDAC3,and HDAC9,while Western blotting detected the protein expression of Brg1,HDAC3,HDAC9,and MOF in rat’s colon ***:The model group showed significantly increased diarrhea score,occult blood score,macroscopic damage score of colon tissue,and colon macroscopic damage index(CMDI)score,as well as elevated mRNA expression levels of HDAC3 and HDAC9,protein expression levels of HDAC3,HDAC9,and MOF,and decreased mRNA and protein expression levels of Brg1 compared to the normal group(P<0.01).In contrast,compared to the mod

关键词： Acupuncture Therapy Moxibustion Therapy Medicinal Cake-insulated Moxibustion Crohn Disease Inflammatory Bowel Diseases Chromatin Remodeling Rats

关节镜下前交叉韧带重建术后早期疼痛管理的研究进展

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中国修复重建外科杂志 2024年第2期38卷 218-225页

作者：余鸿施政良熊波涵李雅娟秦艺华董开颜李彦林李黎昆明医科大学第一附属医院运动医学科昆明650032

目的总结前交叉韧带重建(anterior cruciate ligament reconstruction,ACLR)术后早期疼痛管理方式,为促进ACLR术后功能康复选择合理且有效的疼痛管理方案。方法广泛查阅近年国内外关于ACLR术后早期疼痛管理方案相关文献,并从改善术后疼... 详细信息

目的总结前交叉韧带重建(anterior cruciate ligament reconstruction,ACLR)术后早期疼痛管理方式,为促进ACLR术后功能康复选择合理且有效的疼痛管理方案。方法广泛查阅近年国内外关于ACLR术后早期疼痛管理方案相关文献,并从改善术后疼痛效果方面进行综述。结果目前物理治疗和口服药物有经济、简便等优势,但改善术后疼痛效果并不佳,常需要联合静脉注射或静脉泵等方式,也是目前缓解疼痛的常用方式;但为了满足患者镇痛需求,镇痛药物使用量往往较大,增加了各种不良反应发生风险。局部浸润镇痛(local infiltration analgesia,LIA)包括关节周围或关节内注射药物,可显著改善ACLR术后早期疼痛,达到与神经阻滞相似的术后疗效;LIA可作为代替神经阻滞的一种镇痛技术,并避免神经阻滞带来的相应支配肌肉无力,从而增加术后跌倒风险。其中关节周围注射镇痛效果更优,可避免关节内注射对软骨损伤的风险。但LIA的术后镇痛效果和时效性仍有待提升,可考虑将多模式混合药物LIA(联合关节内和关节周围)联合其他疼痛干预方式,发挥协同作用,以避免单一药物或单途径给药带来的副作用和风险,有望成为缓解ACLR术后早期疼痛常用方法之一。结论关节镜下ACLR术后仍会出现早期疼痛影响患者进一步功能活动,各种镇痛方式均可获得一定疗效,但目前尚未制定统一标准,各类镇痛方式优劣有待进一步研究。

关键词：前交叉韧带重建早期疼痛局部浸润镇痛神经阻滞功能康复

Modelling and mapping soil erosion potential in China

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Journal of Integrative Agriculture 2019年第2期18卷 251-264页

作者： TENG hong-fen HU Jie ZHOU Yue ZHOU Lian-qing shi Zhou College of Environmental and Resource Sciences Zhejiang UniversityHangzhou 310058P.R.China State Key Laboratory of Soil and Sustainable Agriculture Chinese Academy of SciencesNanjing 210008P.R.China

Soil erosion is an important environmental threat in ***,quantitative estimates of soil erosion in China have rarely been reported in the *** this study,soil loss potential in China was estimated by integrating satellite images,field samples,and ground observations based on the Revised Universal Soil Loss Equation(RUSLE).The rainfall erosivity factor was estimated from merged rainfall data using Collocated CoKriging(ColCOK)and downscaled by geographically weighted regression(GWR).The Random Forest(RF)regression approach was used as a tool for understanding and predicting the relationship between the soil erodibility factor and a set of environment *** results show that the average erosion rate in China is 1.44 t ha^(–1) yr^(–1).More than 60%of the territory in China is influenced by soil erosion limitedly,with an average potential erosion rate less than 0.1 t ha^(–1) yr^(–1).Other unused land and other forested woodlands showed the highest erosion *** estimates are comparable to those of runoff plot *** results provide a useful tool for soil loss assessments and ecological environment protections.

关键词： soil erosion potential RUSLE mapping modelling

极端天气下轨道交通水害致灾因子时空分布特征

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人民长江 2024年第7期55卷 10-19页

作者：江雨潮高燕史天根孙可天施旭中山大学地球科学与工程学院广东珠海519000 南方海洋科学与工程广东省实验室(珠海) 广东珠海519000

近年来,在与气候变化相关的多种极端天气因素的相互作用下,洪涝事件对城市及轨道交通系统造成了严重影响。以粤港澳大湾区典型沿海城市洪涝事件为例,研究了轨道交通水害的主要致灾因子(极端降雨和台风风暴潮)及相关特征参数,揭示了其时... 详细信息

近年来,在与气候变化相关的多种极端天气因素的相互作用下,洪涝事件对城市及轨道交通系统造成了严重影响。以粤港澳大湾区典型沿海城市洪涝事件为例,研究了轨道交通水害的主要致灾因子(极端降雨和台风风暴潮)及相关特征参数,揭示了其时空分布特征。研究表明:①近年来,广州市降雨量、降雨强度均明显增加,但降雨历时减少,降雨整体呈现增多且趋于集中的极端化趋势。广州市降雨时间分布在年内存在两个极值区间,第一极值区间与广州市“龙舟水”相关,第二极值区间主要受台风影响。广州市降雨空间分布不均,主要受季风及地理位置影响。②近10 a影响广东省的29场台风风暴潮灾害集中发生在7~10月,该时间与降雨第二极值区间重合。在对近年广东省典型轨道交通水害事件进行总结后发现,水害高发期与两个极值区间重合。虽然广东省台风强度存在缓慢降低趋势,但由于广东省沿海城市遭受台风风暴潮灾害的频次不断上升,且粤中地区密集分布有广东省全部的地下轨道交通系统,未来广东省轨道交通系统水害风险将随着极端天气时空分布特征的变化进一步加大。研究成果可为未来城市轨道交通水害致灾特征研究提供依据,为城市防灾减灾提供参考。

关键词：轨道交通洪涝灾害致灾因子极端降雨台风风暴潮特征参数模型广州市

Effects of thiamine on Trichothecium and Alternaria rots of muskmelon fruit and the possible mechanisms involved

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Journal of Integrative Agriculture 2017年第11期16卷 2623-2631页

作者： GE Yong-hong LI Can-ying Lü Jing-yi ZHU Dan-shi College of Food Science and Engineering Bohai University/Food Safety Key Laboratory of Liaoning Province~National & LocalJoint Engineering Research Center of Storage Processing and Safety Control Technology for Fresh Agricultural and AquaticProducts Jinzhou 121013 P.R. China

The effects of thiamine against pink and black spot rots caused by Trichothecium roseum and Alternaria alternata and modulation on the metabolism of reactive oxygen species （ROS） and phenylpropanoid pathway were investigated in this paper. In vitro test indicated that thiamine significantly inhibited mycelia growth and spore germination of T. roseum and A. alternata. Thiamine at 100 mmol L-1 effectively inhibited lesion development of muskmelon fruit inoculated with T. roseum or A. alternata, enhanced production rate of O2; and H2O2 content, activities of catalase （CAT）, ascorbate peroxidase （APX） and glutathione reductase （GR） in muskmelon fruit. Thiamine also affect phenylpropanoid pathway in muskmelon fruit by increasing the activities of phenylalanine ammonia lyase （PAL） and peroxidase （POD）, the content of total phenolic compounds, flavonoids and lignin. These results suggest that the effects of thiamine on pink and black spot rots in muskmelon fruits are associated with its direct fungitoxic against the pathogens and the modulation of O2- and H2O2 production, elimi- nating enzymes and phenylpropanoid pathway.

关键词： induced resistance thiamine muskmelon fruit antimicrobial activity phenylpropanoid pathway

Virtual machine placement optimizing to improve network performance in cloud data centers

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The Journal of China Universities of Posts and Telecommunications 2014年第3期21卷 62-70页

作者： DONG Jian-kang WANG hong-bo LI Yang-yang CHENG shi-duan State Key Laboratory of Networking and Switching Technology Beijing University of Posts and Telecommunications

With the wide application of virtualization technology in cloud data centers, how to effectively place virtual machine （VM） is becoming a major issue for cloud providers. The existing virtual machine placement （VMP） solutions are mainly to optimize server resources. However, they pay little consideration on network resources optimization, and they do not concern the impact of the network topology and the current network traffic. A multi-resource constraints VMP scheme is proposed. Firstly, the authors attempt to reduce the total communication traffic in the data center network, which is abstracted as a quadratic assignment problem; and then aim at optimizing network maximum link utilization （MLU）. On the condition of slight variation of the total traffic, minimizing MLU can balance network traffic distribution and reduce network congestion hotspots, a classic combinatorial optimization problem as well as NP-hard problem. Ant colony optimization and 2-opt local search are combined to solve the problem. Simulation shows that MLU is decreased by 20%, and the number of hot links is decreased by 37%.

关键词： cloud computing data center network virtual machine placement traffic engineering network performance

益蝽成虫肠道微生物群落结构及其碳源代谢功能

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昆虫学报 2024年第8期67卷 1063-1074页

作者：李金梦孙智荣田旭汪汉成周凤史彩华李文红长江大学农学院荆州434025 贵州省农业科学院植物保护研究所贵阳550006 贵州省烟草科学研究院贵阳550081 贵州省烟草公司黔西南州公司兴义562400 长江大学生命科学学院荆州434025 湖北文理学院现代农业研究院襄阳441053

【目的】肠道微生物对宿主昆虫的代谢、生长发育及免疫等功能发挥着重要作用。本研究旨在探究益蝽Picromerus lewisi成虫肠道微生物群落结构及其碳源代谢能力。【方法】利用体外培养分离纯化益蝽成虫肠道微生物并进行分子生物学鉴定,利... 详细信息

【目的】肠道微生物对宿主昆虫的代谢、生长发育及免疫等功能发挥着重要作用。本研究旨在探究益蝽Picromerus lewisi成虫肠道微生物群落结构及其碳源代谢能力。【方法】利用体外培养分离纯化益蝽成虫肠道微生物并进行分子生物学鉴定,利用Illumina高通量测序技术进行细菌16S rDNA和真菌ITS基因测序,解析益蝽成虫肠道细菌和真菌群落结构和多样性;利用PICRUSt和FUNGuild对细菌和真菌及其基因功能进行预测;利用Biolog ECO技术解析益蝽成虫肠道细菌和真菌的碳源代谢功能。【结果】共分离益蝽成虫肠道可培养优势细菌肠球菌属Enterococcus菌株10株,优势细菌为粪肠球菌***。高通量测序结果表明,在门水平上,益蝽成虫肠道优势细菌为变形菌门(Proteobacteria)(相对丰度58.51%)和厚壁菌门(Firmicutes)(相对丰度38.92%);主要优势真菌为子囊菌门(Ascomycota)(相对丰度56.53%),其次是担子菌门(Basidiomycota)(相对丰度11.34%)。在属水平上,肠球菌属Enterococcus(相对丰度25.05%)为主要优势细菌属,其次是乳球菌属Lactococcus(相对丰度12.23%)、沙雷氏菌属Serratia(相对丰度11.48%)和普罗威登斯菌属Providencia(相对丰度2.38%);优势真菌属主要为Biappendiculispora(相对丰度18.30%),其次是枝孢菌属Cladosporium(相对丰度11.83%)、Vishniacozyma(相对丰度5.17%)和鬼笔属Phallus(相对丰度3.62%)。益蝽成虫肠道细菌和真菌群落结构具有较高的物种丰富度和多样性,以及较强的碳源代谢能力,可高效代谢α-环式糊精、L-丝氨酸、腐胺和D-苹果酸等27种碳源。功能预测显示,益蝽成虫肠道细菌主要分布于新陈代谢类群、环境信息处理类群和遗传信息处理类群等,真菌主要分布于未定义类群、植物腐生菌、未定义腐生菌、内生菌-植物病原菌、真菌寄生菌-未定义腐生菌、叶子腐生菌和动物病原菌-内生菌-植物病原菌-木质腐生菌类。【结论】益蝽成虫肠道细菌和真菌种类多、多样性高,在属水平,优势细菌为肠球菌属、乳球菌属、沙雷氏菌属和普罗威登斯菌属,优势真菌为Biappendiculispora、枝孢菌属、Vishniacozyma和鬼笔属,具有较强的碳源代谢能力。

关键词：益蝽肠道微生物群落结构碳源代谢高通量测序

A novel beta-galactose-specific lectin of the tubeworm,Ridgeia piscesae,from the hydrothermal vent

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Acta Oceanologica Sinica 2017年第6期36卷 61-67页

作者： RUAN Lingwei XU Haipeng LIN Wenyang shi hong CUI Zhizhong XU Xun State Key Laboratory Breeding Base of Marine Genetic Resources Xiamen 361005China Key Laboratory of Marine Genetic Resources of State Oceanic Administration Third Institute of OceanographyState Oceanic AdministrationXiamen 361005China Fujian Key Laboratory of Marine Genetic Resources Xiamen 361005China Animal Science and Technology College Shandong Agricultural UniversityTai'an 271018China

Lectins are sugar-specific binding proteins or glycoproteins that play important physiological roles in cellular recognition and regulation. And they are also valuable in medicine and pharmacy. Tubeworm is the representative species around the hydrothermal vent in the deep sea. They have developed unique mechanisms to adapt to the harsh environment. In this study, a 1 092 bp cDNA, designed as rpgal, was first cloned and characterized from the tubeworm Ridgeia piscesae. Sequence analysis showed that RPGAL had low homology with the known galectin. And it had two homologous carbohydrate-recognition domains, which is the characteristic of the tandem-repeat type galectins. The RPGAL was successfully recombinant expressed in Escherichia coli and purified. Analysis of biological activity revealed that RPGAL was metal ion independent and it could agglutinate all the vertebrate erythrocytes tested. It was stable at 10-50℃ and pH 5-10. And the hemagglutinating activity of RPGAL was strongly inhibited by D-Lactose and lipopolysaccharide. Although RPGAL had no effect on the microorganisms tested, it showed anti-tumor activity towards HeLa cells and HT1080 cells, which was accomplished by apoptosis. The study demonstrated that RPGAL was a novel galectin and provided a potential candidate for therapy of anti-tumor.

关键词： galectin antitumor apoptosis tubeworm Ridgeia piscesae

TIMP-3 gene transfection suppresses invasive and metastatic capacity of human hepatocarcinoma cell line HCC-7721

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Hepatobiliary & Pancreatic Diseases International 2007年第5期6卷 487-491页

作者： Zhang, hong Wang, Yue-Shu Han, Gang shi, Ying Jilin Univ Second Hosp Dept Gen Surg Changchun 130041 Peoples R China Jilin Univ Dept Hand Surg China Japan Union Hosp Changchun 130051 Peoples R China

BACKGROUND: Tissue inhibitor of metalloproteinases (TIMPs) can restrain the tumor growth by their protein property and matrix metalloproteinases (MMPs) inhibition. There are currently four known TIMP family members-TIMP-1, 2, 3, 4. We determined whether increasing levels of TIMP-3 expression could suppress the malignant phenotype of human hepatocarcinoma cell line HCC-7721. METHODS: A recombinant expression vector, which contained full-length cDNA of human TIMP-3, was constructed and transfected into the human hepatocarcinoma cell line HCC-7721 by the lipofectamine technique. In vitro and in vivo tests such as Western blotting, immunohistochemistry as well as xenografting in nude mice were used to analyze expression levels of TIMP and MMP, and changes in malignant phenotype after the gene transfection. RESULTS: TIMP-3 expression in TIMP-3 gene-transfected HCC-7721 cells was upregulated as assessed by Western blotting. The ability of in vitro invasion through a Boyden chamber was significantly decreased compared to controls. Following subcutaneous injection into nude mice, the TIMP-3 transfected cells suppressed primary tumor growth, as characterized by reduced tumor weight, size and microvasculature as well as maintaining the extracellular matrix. CONCLUSION: The results suggest that upregulation of TIMP-3 expression in HCC-7721 cells inhibits invasion capacity in vitro as well as tumorigenic and metastatic potential in nude mice.

关键词： hepatocarcinoma metalloproteinases neoplasm invasion