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High expression of the circadian clock gene NPAS2 is associated with progression and poor prognosis of gastric cancer:A singlecenter study

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World Journal of Gastroenterology 2023年第23期29卷 3645-3657页

作者： xiao-Meng Cao Wen-Di Kang tian-hong xia Shao-Bin Yuan Chang-An Guo Wen-Jie Wang hong-Bin Liu Department of General Surgery Gansu Provincial Hospital of TCMLanzhou 730050Gansu ProvinceChina The First Clinical Medical College Gansu University of Chinese MedicineLanzhou 730030Gansu ProvinceChina Department of Interventional Therapy National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Clinical Medicine College Ningxia Medical UniversityClinical Medicine collegeNingxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Department of Emergency Lanzhou University Second HospitalLanzhou 730030Gansu ProvinceChina Department of General Surgery Lanzhou University Second HospitalLanzhou 730030Gansu ProvinceChina Department of General Surgery The 940^(th)Hospital of Joint Logistics Support Force of Chinese People's Liberation ArmyLanzhou 730050Gansu ProvinceChina

BACKGROUND The prognostic assessment of patients after surgical resection of gastric cancer(GC)patients is ***,the role of the circadian clock gene NPAS2 expression in GC remains *** To explore the relationship between NPAS2 and the survival prognosis of GC patients and clarify its role in evaluating GC *** The tumor tissues and clinical data of 101 patients with GC were collected *** staining(IHC)was used to detect the expression of NPAS2 protein in GC and adjacent *** and multivariate Cox regression analysis was used to determine the independent prognostic factors of GC,and a nomogram prediction model was *** receiver operating characteristic(ROC)curve,the ROC area under the curve,the calibration curve,and C-index were used to evaluate the predictive effectiveness of the *** Meier analysis was used to compare the risk stratification of subgroups according to the median score in the nomogram model of each *** Microarray IHC analysis showed that the positive rate of NPAS2 protein expression in GC tissues was 65.35%,which was significantly higher than 30.69%in adjacent *** high expression of NPAS2 was correlated with tumor-node-metastasis(TNM)stage(P<0.05),pN stage(P<0.05),metastasis(P<0.05),venous invasion(P<0.05),lymphatic invasion(P<0.05),and lymph node positive(P<0.05)of *** Meier survival analysis showed that the 3-year overall survival(OS)of patients with high NPAS2 expression was significantly shortened(P<0.0001).Univariate and multivariate COX regression analysis showed that TNM stage(P=0.009),metastasis(P=0.009),and NPAS2 expression(P=0.020)were independent prognostic factors of OS in GC patients for 3 *** nomogram prediction model based on independent prognostic factors has a C-Index of 0.740(95%CI:0.713-0.767).Furthermore,subgroup analysis showed that the 3-year OS time of the high-risk group was significantly lower than th

关键词： NPAS2 Gastric cancer Tissue microarray Survival analysis Prediction model Nomogram

Pregnancy and fetal outcomes of chronic hepatitis C mothers with viremia in China

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World Journal of Gastroenterology 2022年第34期28卷 5023-5035页

作者： Calvin Q Pan Bao-Shen Zhu Jian-Ping Xu Jian-xia Li Li-Juan Sun hong-xia tian Xi-hong Zhang Su-Wen Li Er-Hei Dai Center for Liver Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Division of Gastroenterology and Hepatology Department of MedicineNYU Langone HealthNYU School of MedicineFlushingNY 11355United States Department of Obstetrics and Gynecology The Fifth Hospital of ShijiazhuangHebei Medical UniversityShijiazhuang 050021Hebei ProvinceChina School of Public Health North China University of Science and TechnologyTangshan 063210Hebei ProvinceChina Division of Liver Disease Department of MedicineThe Fifth Hospital of ShijiazhuangHebei Medical UniversityShijiazhuang 050021Hebei ProvinceChina

BACKGROUND Data that assess maternal and infant outcomes in hepatitis C virus(HCV)-infected mothers are *** To investigate the frequency of complications and the associated risk *** We performed a cohort study to compare pregnancy and fetal outcomes of HCVviremic mothers with those of healthy *** factors were analyzed with logistic *** Among 112 consecutive HCV antibody-positive mothers screened,we enrolled 79 viremic *** randomly selected 115 healthy mothers from the birth registry as the *** to healthy mothers,HCV mothers had a significantly higher frequency of anemia[2.6%(3/115)vs 19.0%(15/79),P<0.001]during pregnancy,medical conditions that required caesarian section[27.8%(32/115)vs 48.1%(38/79),P=0.004],and nuchal cords[9.6%(11/115)vs 34.2%(27/79),P<0.001].In addition,the mean neonatal weight in the HCV group was significantly lower(3278.3±462.0 vs 3105.1±459.4 gms;P=0.006),and the mean head circumference was smaller(33.3±0.6 vs 33.1±0.7 cm;P=0.03).In a multivariate model,HCV-infected mothers were more likely to suffer anemia[adjusted odds ratio(OR):18.1,95%confidence interval(CI):4.3-76.6],require caesarian sections(adjusted OR:2.6,95%CI:1.4-4.9),and have nuchal cords(adjusted OR:5.6,95%CI:2.4-13.0).Their neonates were also more likely to have smaller head circumferences(adjusted OR:2.1,95%CI:1.1-4.3)and lower birth weights than the average(≤3250 gms)with an adjusted OR of 2.2(95%CI:1.2-4.0).The vertical transmission rate was 1%in HCV-infected *** Maternal HCV infections may associate with pregnancy and obstetric *** demonstrated a previously unreported association between maternal HCV viremia and a smaller neonatal head circumference,suggesting fetal growth restriction.

关键词： Hepatitis C virus viremia Mother-to-child transmission Pregnancy complications Maternal health Infant hepatitis C virus infection

Analysis of risk factors for postoperative pancreatic fistula following pancreaticoduodenectomy

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World Journal of Gastroenterology 2014年第46期20卷 17491-17497页

作者： Qi-Yu Liu Wen-Zhi Zhang hong-tian xia Jian-Jun Leng Tao Wan Bin Liang Tao Yang Jia-hong Dong Department of Hepatobiliary Surgery the First People’s Hospital of Kunming Department of Hepatobiliary Surgery Chinese People’s Liberation Army General Hospital

AIM: To explore the morbidity and risk factors of postoperative pancreatic fistula (POPF) following pancreaticoduodenectomy.

关键词： Pancreaticoduodenectomy Pancreatic fistula Complication Pancreatic duct

Establishment and application of a multiplex genetic mutation-detection method of lung cancer based on MassARRAY platform

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Cancer Biology & Medicine 2016年第1期13卷 68-76页

作者： hong-xia tian Xu-Chao Zhang Zhen Wang Jian-Guang Chen Shi-Liang Chen Wei-Bang Guo Yi-Long Wu Medical Research Center Guangdong Lung Cancer Institute Guangdong General Hospital Guangdong Academy of Medical Sciences

Objective: This study aims to establish a method for highly parallel multiplexed detection of genetic mutations in Chinese lung cancer samples through Agena i PLEX chemistry and matrix-assisted laser desorption ionization time-of-flight analysis on Mass ARRAY mass spectrometry ***: We reviewed the related literature and data on lung cancer treatments. We also identified 99 mutation hot spots in 13 target genes closely related to the pathogenesis, drug resistance, and metastasis of lung cancer. A total of 297 primers, composed of99 paired forward and reverse amplification primers and 99 matched extension primers, were designed using Assay Design software. The detection method was established by analyzing eight cell lines and six lung cancer specimens. The proposed method was then validated through comparisons by using a Lung Carta^(TM) kit. The sensitivity and specificity of the proposed method were evaluated by directly sequencing EGFR and KRAS genes in 100 lung cancer ***: The proposed method was able to detect multiplex genetic mutations in lung cancer cell lines. This finding was consistent with the observations on previously reported mutations. The proposed method can also detect such mutations in clinical lung cancer specimens. This result was consistent with the observations with Lung Carta^(TM) kit. However, an FGFR2 mutation was detected only through the proposed method. The measured sensitivity and specificity were 100% and 96.3%, ***: The proposed Mass ARRAY technology-based multiplex method can detect genetic mutations in Chinese lung cancer patients. Therefore, the proposed method can be applied to detect mutations in other cancer tissues.

关键词： Lung neoplasms driver genes mutation multigene testing MassARRAY

Establishment of a Novel Method for Screening Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Resistance Mutations in Lung Cancer

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Chinese Medical Journal 2017年第12期130卷 1446-1453页

作者： hong-xia tian Xu-Chao Zhang Zhen Wang Jin-Ji Yang Wei-Bang Guo Zhi-hong Chen Yi-Long Wu Medical Research Center Guangdong Lung Cancer Institute Guangdong General Hospital Guangdong Academy of Medical Sciences Guangzhou Guangdong 510080 China Division of Pulmonary Oncology Guangdong Lung Cancer Institute Guangdong General Hospital Guangdong Academy of Medical Sciences Guangzhou Guangdong 510080 China

Background： Drug resistance to targeted therapies occurs in lung cancer, and resistance mechanisms related to epidermal growth factor receptor （EGFR） tyrosine kinase inhibitors （TKIs） are continuously being discovered. We aimed to establish a novel method for highly parallel multiplexed detection of genetic mutations related to EGFR TKl-resistant lung cancer using Agena iPLEX chemistry and niatrix-assisted laser desorption ionization time-of-flight analysis on the MassARRAY mass spectrometry platform. Methods： A review of the literature revealed 60 mutation hotspots in seven target genes （EGFR, KRAS, PIK3CA, BRAF, ERBB2, NRAS, and BIM） that are closely related to EGFR TKI resistance to lung cancer. A total of 183 primers comprised 61 paired forward and reverse amplification primers, and 61 matched extension primers were designed using Assay Design Software. The detection method was established by analyzing nine cell lines, and by comparison with LungCartaTM kit in ten lung cancer specimens. EGFR, KRAS. and BIM genes in all cell lines and clinical samples were subjected to Sanger sequencing for confirming reproducibility. Results： Our data showed that designed panel was a high-throughput and robust tool, allowing genotyping for sixty hotspots in the same run. Moreover, it made efficient use of patient diagnostic samples for a more accurate EGFR TKIs resistance analysis. The proposed method could accurately detect mutations in lung cancer cell lines and clinical specimens, consistent with those obtained by the LungCartaTM kit and Sanger sequencing. We also established a method for detection of large-fragment deletions based on single-base extension technology of MassARRAY platform. Conclusions： We established an effective method for high-throughput detection of genetic mutations related to EGFR TKI resistance based on the MassARRAY platform, which could provide more accurate information for overcoming cancers with de novo or acquired resistance to EGFR-targeted therap

关键词： Drug Resistance Epidermal Growth Factor Receptor Lung Cancer MassARRAY Targeted Molecular Therapy

基于两次改进灰色加权马尔可夫模型的CPI预测

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中北大学学报（自然科学版） 2015年第2期36卷 113-117页

作者：田红霞中北大学理学院山西太原030051

利用灰色加权马尔可夫预测模型对居民消费价格指数（CPI）进行研究，并在此基础上进行两次改进.首先利用无偏灰色加权马尔可夫模型进行改进，消除了原有模型固有的偏差；又利用递进转移概率矩阵进行二次改进，在预测时增加最新的预测数... 详细信息

利用灰色加权马尔可夫预测模型对居民消费价格指数（CPI）进行研究，并在此基础上进行两次改进.首先利用无偏灰色加权马尔可夫模型进行改进，消除了原有模型固有的偏差；又利用递进转移概率矩阵进行二次改进，在预测时增加最新的预测数据，去掉距离最远的数据，最后结合2013.06~2014.03的 CPI数据对2014年4月及5月份的数据进行预测.结果表明：两次改进的灰色加权马尔可夫模型与一般灰色加权马尔可夫模型相比，平均相对误差约减少30%，效果较好.

关键词：灰色加权马尔可夫无偏灰色加权马尔可夫递进转移概率矩阵 CPI 预测