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AtbHLH2. of Arabidopsis thaliana is a functional ortholog of tomato FER involved in controlling iron acquisition in strategy I plants

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Cell Research 2005年第8期15卷 613-621页

作者： You Xi YUAN1,2. juan ZHANG1,2. Dao Wen WANG1, hong Qing {2.NG1,* 1The State key laboratory of Plant Cell and Chromosome Engineering, institute of genetics and developmental biology, Chinese academy of Sciences, Datun road, chaoyang district, beijing 1.01.1, china 2.raduate School of the Chinese academy of Sciences, Yuquan road, Shijingshan district, beijing 100039, china The State key {1. of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China Graduate School of the Chinese academy of Sciences Beijing China

AtbHLH2. of Arabidopsis, encoding a bHLH protein, reveals a high similarity to the tomato FER which is proposed as a transcriptional regulator involved in controlling the iron deficiency responses and the iron uptake in tomato. For identification of its biological functions, AtbHLH2. was introduced into the genome of the tomato FER mutant T32.8fer mediated by Agrobacterium tumefaciencs. Transgenic plants were regenerated and the stable integration of AtbHLH2. into their genomes was confirmed by Southern hybridization. {1. analysis demonstrated that expression of the exogenous AtbHLH2. of Arabidopsis in roots of the FER mutant T32.8fer enabled to complement the defect functions of FER. The transgenic plants regained the ability to activate the whole iron deficiency responses and showed normal growth as the wild type under iron-limiting stress. Our transformation data demonstrate that AtbHLH2. is a functional ortholog of the tomato FER and can completely replace FER in controlling the effective iron acquisition in tomato. Except of iron, FER protein was directly or indirectly involved in manganese homeostasis due to that loss functions of FER in T32.8fer resulted in strong reduction of Mn content in leaves and the defect function on Mn accumulation in leaves was complemented by expression of AtbHLH2. in the transgenic plants. Identification of the similar biological functions of FER and AtbHLH2., which isolated from two systematically wide-diverged “strategy I” plants, suggests that FER might be a universal gene presented in all strategy I plants in controlling effective iron acquisition system in roots.

关键词： tomato AtbHLH2. iron uptake Arabidopsis FER plant nutrition.

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molecular and functional characterization of sulfiredoxin homologs from higher plants

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Cell Research 2006年第3期16卷 287-296页

作者： Xian Peng liu~(1,2. Xue Ying liu~(1,2. {2.an Zhang~(1,2. Zong liang Xia~(1,2. Xin liu~1 Huan {2. Qin~1 Dao Wen Wang~11 The State key laboratory of Plant Cell and Chromosome Engineering,institute of genetics and developmental biology,Chinese academy of Sciences,Datun road,chaoyang district,{12. 2.Graduate University of Chinese academy of Sciences,Yuquan road,Shijingshan district,beijing 100039,china The State key {1. of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences Chaoyang District China Graduate University of Chinese academy of Sciences Shijingshan District China

By reducing cysteine-sulfinic acid in oxidized peroxiredoxin,sulfiredoxin (Srx) plays an important role in oxidationstress resistance in yeast and human ***,we report the first molecular and functional characterization of Srxhomolog from higher *** analysis revealed the presence of potential Srx encoding sequences in bothmonocot and dicot plant *** plant Srx proteins exhibited significant identities to their orthologs from yeastand human,and contained the conserved signature sequence and residues essential for ***,un{1.ke yeastand human orthologs,plant Srxs were all predicted to possess chloroplast transit peptide in their primary *** proteins from Arabidopsis and rice (designated as AtSrx and OsSrx,respectively) complemented functional defi-ciency of Srx in the SRXl deletion yeast cells.A GFP fusion protein of AtSrx was targeted to chloroplast in Arabidopsismesophyll *** transcription occurred in both vegetative and reproductive organs,and the highest transcriptlevel was detected in *** oxidation stress,AtSrx transcript level was substantially increased,which paralleledwith enhanced transcription of 2.Cys peroxiredoxins that have been found essential in maintaining chloroplast *** addition to oxidation stress,osmotic/water deficit or cold treatments also raised AtSrx transcript ***-sistent with above findings,the knock-out mutant of AtSrx was significantly more susceptible to oxidation stress thanwild type Arabidopsis *** together,the results of this work indicate the existence of functional Srx homolog inhigher plants that is essential for plants to cope with oxidation stress.

关键词： Arabidopsis sulfiredoxin chloroplast reactive oxygen species stress

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Micropylar guidancel (mg1. MG1.is essential for pollen tube

Micropylar guidancel (mg1) MG1 is essential for pollen tube

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中国植物学会植物细胞生物学2.1.年学术年会

作者： hong-ju,li and wei-cai,{2.~* The key laboratory of molecular and developmental biology,institute of genetics and developmental biology,Chinese academy of Sciences,1.west beichen road,chaoyang district,{12.

In flowering plants,precise guidance of the pollen tube through the female reproductive tissues to the embryo sac is critical for sexual *** tube guidance by which pollen tube find the entry to the embryo sac,is a common phenomenon in angiosperms and requires coordinated male-female gametophytic interactions,but the underlying molecular mechanism is poorly understood. The entire process of pollen tube guidance can be divided into two phases:the sporophytic phase and gametophytic phase,and the latter include funicular phase and micropylar *** are multiple guidance cues along the pollen tube pathway,and the pollen tube employs different mechanism to receive and respond to the *** there are evidences that the synergid in ovule emits diffusible attractants,how the pollen tube responds to them is still unknown.

关键词： Arabidopsis MG1 Pollen tube guidance

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PODl,a novel ER protein,controls the pollen tube response to female cues in Arabidopsis

PODl,a novel ER protein,controls the pollen tube response to...

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2.1.全国植物生物学研讨会

作者： hongju {2.,Yong Xue,Tong Wang,weicai yang~* key laboratory of molecular and developmental biology,and National Center for Plant Gene Research(beijing),{12.,Chinese academy of Sciences, 1.west beichen road,beijing 1.01.1.china

Pollen tube guidance is a common process,analogous to axon guidance in animals,which involves cell-cell interactions and signaling between the male gametophyte(pollen) and the female *** putative female cues to attract pollen tube have been shown to be Cys-rich defensin-like peptide(Okuda et al., Nature 2.09).Nothing is known on how pollen tube perceives and responds to the female cues although a putative membrane K transporter was implicated in this process(Lu et al.,Plant Cell 2.1.).In an effort for male mutations disrupting the perception of the female signals,we identified a pollen-specific mutation,

关键词： Arabidopsis pollen tube guidance POD1 ER quality control

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Encodes a putative alkaline phytoceramidase that is important for pollen tube and female gametophyte interaction

Encodes a putative alkaline phytoceramidase that is importan...

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中国植物学会植物细胞生物学2.1.年学术年会

作者： li-Yu Chen,Wen-juan Zhang,Dong-Qiao Shi,Jie liu,and wei-cai yang~* The key laboratory of molecular And developmental biology,institute of genetics and developmental biology, Chinese {12. of Sciences,1.west Baichen road,chaoyang district,beijing 1.01.1.china

In flowering plants,the interaction between pollen tube and female gametophyte plays a critical role for the successful ***,we identified an Arabidopsis mutant,in vivo pollen tube defect 1.ipod1.,from Ds transposon insertion *** mutant displays an obvious phenotype of shorter si{2.ques and a high frequency of aborted *** analysis and {1. characterization demonstrated that the phenotype is caused by a single Ds *** ipodl mutants exhibit a modest reduction in seed set compared with wild-type *** crosses proved that the mutation results in defects of male gametophytic *** phenotypic analysis demonstrated that pollen tube growth in pistil is abnormal while in vitro growth is not *** genetic complementation experiments and additional T-DNA insertion allele analysis showed that the mutant phenotype is caused by the disruption of a putative alka{2.ne phytoceramidase,which is partially coloca{2.zed with the Golgi apparatus and endoplasmic *** carries a central role in sphingo{2.pid metabo{2.sm in eukaryotic cells,which regulates cell pro{2.feration,differentiation,apoptosis and different kinds of stress ***1.gene is mainly expressed in reproductive *** data suggest that iPOD1.functions in the interaction between pollen tube and female gametophyte.

关键词： Arabidopsis iPODl male sterility pollen tube alkaline phytoceramidase

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An Abiotic Stress Inducible Arabidopsis Ubiquitin Conjugating Enzyme UBC6a is Involved in ERAD

An Abiotic Stress Inducible Arabidopsis Ubiquitin Conjugatin...

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植物分子生物学与现代农业——2.1.全国植物生物学研讨会

作者： Feng Cui,Qingzhen Zhao,lijing liu,Z{2.hui Zhang,Yaorong Wu,Sanyuan Tang and Qi Xie~* State key laboratory of Plant Genomics,National Center for Plant Gene Research,institute of genetics and developmental biology,Chinese academy of Sciences,No.1.west beichen road,chaoyang district,beijing 1.01.1.china

Ubiquitination plays important roles in plant adapting itself to internal and external signals to regulate plant growth and *** model plant Arabidopsis (Arabidopsis thaliana) genome size is about 1.0 Mbs and it contai... 详细信息

关键词： UBC6a ERAD ER stress ubiquitin

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Characterization of low molecular {1.ght glutenin subunit genes at Glu-3 loci in common wheat

Characterization of low molecular weight glutenin subunit ge...

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中国遗传学会植物遗传和基因组学专业委员会2.07年学术研讨会

作者： lingli Dong Xiaofei Zhang Dongcheng liu Aimin Zhang hong-Qing ling The State key laboratory of Plant Cell and Chromosome Engineering,institute of genetics and developmental biology,Chinese academy of Sciences,Datun road,chaoyang district,beijing 1.01.1.china

Low-molecular-{1.ght glutenin subunits (LMW-GS) account for 1.3 of the preserved proteins in wheat seed and play a crucial role in the processing properties of *** are encoded by genes at Glu-3 ***,little is known about their ***,we systematically studied the LMW-GS genes of an elite common wheat cultivar Xiaoyan 54 at the genornic,transcriptional and protein levels.

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An abiotic stress inducible Arabidopsis ubiquitin conjugation enzyme UBC32.is involved in ERAD

An abiotic stress inducible Arabidopsis ubiquitin conjugatio...

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植物分子生物学与现代农业——2.1.全国植物生物学研讨...

作者： Feng Cui~1,Qingzhen Zhao~(1,2.,lijing liu~1,Z{2.hui Zhang~1,Ran Xia~1,Yaorong Wu~1,Huawei Zhang~1,Huishan Guo~3 and Qi Xie~(1,4*) 1 State key laboratory of Plant Genomics,National Center for Plant Gene Research,institute of genetics and developmental biology,Chinese academy of Sciences,No.1.west beichen road,chaoyang district,beijing 1.01.1.china 2.School of life Science,liaocheng University,liaocheng 2.2.59,china 3 State key laboratory of Plant Genomics,National Center for Plant Gene Research,institute of Microbiology,Chinese academy of Sciences,No.1.west beichen road,chaoyang district,beijing 1.01.1.china

Ubiquitin- proteasome system plays important roles in plant growth,development, responses to stresses and plant *** progresses have been achieved about the research area in recent *** Reticulum Associated Protein Degr... 详细信息

关键词： UBC32 ERAD ER stress ubiquitin

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Pestalotiopsis fici genome reveals the richness of secondary metabolites of endophytic fungus

Pestalotiopsis fici genome reveals the richness of secondary...

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2.1.年中国菌物学会学术年会

作者： Xiuna Wang~(1 2) ling liu~1 Xinyu Zhang~1 Gang liu~1 liangdong Guo~1 Yongsheng Che~1 Chengshu Wang~3 Zhiqiang An~4 liyun Guo~2 Xingzhong liu~(1*) (1 State key laboratory of Mycology institute of Microbiology Chinese academy of Sciences No 3 1~(st) west beichen Rd chaoyang district beijing 1.01.1.china 2 Department of Plant Pathology china Agricultural University beijing 100094 china 3 key laboratory of Insect developmental and Evolutionary biology institute of Plant physiology and Ecology Shanghai institutes for Biological Sciences Chinese academy of Sciences Shanghai 200032 china 4 institute of molecular Medicine University of Texas Health Science Center at Houston Houston TX 77030 USA) Department of Plant Pathology China Agricultural University Beijing 100094 China State Key Laboratory of Mycology Institute of Microbiology Chinese Academy of SciencesNo 3 1st West Beichen Rd Chaoyang District Beijing 100101 China Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Institute of Molecular Medicine University of Texas Health Science Center at Houston HoustonTX 77030 USA Department of Plant Pathology China Agricultural University Beijing 100094 China State key {1. of Mycology Institute of Microbiology Chinese Academy of SciencesNo 3 1st West Beichen Rd Chaoyang District Beijing 100101 China

＜正＞Endophytic fungi grow within their plant hosts without causing apparent disease symptoms and are an important source of novel bioactive secondary metabolites（Schulz,*** C. Boyle 2.06,Strobel,G.,***,et al.2.04）.Pestalotiopsis fici,an important woody plant endophyte,produces numerous bioactive secondary metabolites（liu,L 2.1.）.To reveal the mystery of so many secondary metabolites,the genome of *** was shotgun sequenced using a Roche 454 GS FLX *** is a 51.91.Mb genome size containing～1.,063 predicted protein-encoding *** indicated that the gene clusters for secondary metabolites were significantly expanded in number and diversity comparing with other fungal *** protein families related to secondary metabolism,such as MFS general substrate transporter, aromatic compound dioxygenase,cytochrome P450,FAD-binding domain oxidase,ankyrin repeat, Zn2.Cys6 DNA-binding domain *** more abundant in the genome of *** based on the Pfam and CAFE *** preliminary analysis also found evidence for horizontal transfer of a secondary metabolite gene cluster from host plants to *** research explained that the richness of secondary metabolites in endophytic fungi from gene expansion and horizontal transfer of gene clusters.

关键词： Endophytic fungi secondary metabolite genome

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Requirement and Functional Redundancy of Ib Subgroup bHLH Proteins for Iron Deficiency Responses and Uptake in Arabidopsis thaliana

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molecular Plant 2013年第2期6卷 503-513页

作者： Ning Wang Yan Cui Yi liu Huajie Fan {1.an Du Zongan Huang Youxi Yuan Huilan Wu {2.-Qing ling State key {1. of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences No. 1West Beichen Road Chaoyang District Beijing 100101 China Graduate School of theChinese academy of Sciences Yuquan Road Shijingshan District Beijing 100049 China Present address: Department of Biochemistry and molecular {1. Pennsylvania State University University Park PA 16802 USA Present address: institute of Vegetable Crops Wenzhou Academy of Agricultural Sciences Wenzhou Vocation College of Science and TechnologyWenzhou 325014 China

The Ib subgroup of the bHLH gene family in Arabidopsis contains four members （AtbHLH38, AtbHLH39, AtbHLHIO0, and AtbHLH1.1.. AtbHLH38 and AtbHLH39 were previously confirmed to interact with FER-{2.ke iron deft-ciency induced transcription factor （FIT）, directly functioning in activation of the expression of ferric-chelate reductase FRO2.and high-affinity ferrous iron transporter IRT1. In this work, we characterized the functions of AtbHLH1.0 and AtbHLH1.1.in the regulation of the iron-deficiency responses and uptake. Yeast two-hybrid analysis and bi{1. fluorescence complementation assay demonstrated that both AtbHLH1.0 and AtbHLH1.1.could interact with FIT. Dual expression of either AtbHLH1.0 or AtbHLH1.1.with FIT in yeast cells activated the GUS expression driven by promoters of FRO2.and IRT1. The plants overexpressing FIT together with AtbHLHI01.showed constitutive expression of FRO2.and IRT1.in roots, and accumulated more iron in shoots. Further, the single, double, and triple knockout mutants of AtbHLH38, AtbHLH39, AtbHLH1.0, and AtbHLH1.1.were generated and characterized. The FRO2.and IRT1.expression in roots and the iron content in shoots were more drastically decreased in the triple knockout mutant of AtbHLH39, AtbHLH1.0, and AtbHLH1.1.than that of the other available double and triple mutants of the four genes. Comparison of the physiological responses as well as the expression of FRO2.and IRT1.in the multiple knockout mutants under iron deficiency revealed that AtbHLH1.0, AtbHLH38, AtbHLH1.1. and AtbHLH39 played the gradually increased important role in the iron-deficiency responses and uptake. Taken all together, we conclude that the four Ib subgroup bHLH proteins are required and possess redundant functions with differential significance for activation of iron-deficiency responses and uptake in Arabidopsis.

关键词： transcription factor bHLH protein iron homeostasis FIT.

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