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Effects of Adipose-derived Mesenchymal Stem Cell Exosomes on Corneal Stromal Fibroblast Viability and Extracellular Matrix Synthesis

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Chinese Medical Journal 2018年第6期131卷 704-712页

作者： Ting Shen Qing-Qing Zheng Jiang Shen Qiu-Shi Li Xing-Hui Song hong-bo luo Chao-Yang hong Ke Yao Eye Institute of Zhejiang University Eye Center of Second Affiliated Hospital of Zhejiang University School of Medicine Hangzhou Zhejiang 310009 China Department of Ophthalmology Zhejiang Provincial People's Hospital and People's Hospital of Hangzhou Medical College Hangzhou Zhejiang 310014 China School of Ophthalmology and Optometry Wenzhou Medical University Wenzhou Zhejiang 325035 China Facility for Biochemistry and Molecular Medicine Core Facilities School of Medicine Zhejiang University Hangzhou Zhejiang 310058 China

Background： Corneal stromal cells （CSCs） are components of the corneal endothelial microenvironment that can be induced to form a functional tissue-engineered corneal endothelium. Adipose-derived mesenchymal stem cells （ADSCs） have been reported as an important component of regenerative medicine and cell therapy for corneal stromal damage. We have demonstrated that the treatment with ADSCs leads to phenotypic changes in CSCs in vitro. However, the underlying mechanisms of such ADSC-induced changes in CSCs remain unclear. Methods： ADSCs and CSCs were isolated from New Zealand white rabbits and cultured in vitro. An Exosome Isolation Kit, Western blotting, and nanoparticle tracking analysis （NTA） were used to isolate and confirm the exosomes from ADSC culture medium. Meanwhile, the optimal exosome concentration and treatment time were selected. Cell Counting Kit-8 and annexin V-fluorescein isothiocyanate/ propidium iodide assays were used to assess the effect of ADSC-derived exosomes on the proliferation and apoptosis of CSCs. To evaluate the effects ofADSC-derived exosomes on CSC invasion activity, Western blotting was used to detect the expression of matrix metalloproteinases （MMPs） and collagens. Results： ADSCs and CSCs were successfully isolated from New Zealand rabbits. The optimal concentration and treatment time of exosomes for the following study were 100 μg/ml and 96 h, respectively. NTA revealed that the ADSC-derived exosomes appeared as nanoparticles （40-200 nm）, and Western blotting confirmed positive expression of CD9, CDSI, flotillin-1, and HSP70 versus ADSC cytoplasmic proteins （all P〈 0.01 ）. ADSC-derived exosomes （50μg/ml and 100μg/ml） significantly promoted proliferation and inhibited apoptosis （mainly early apoptosis） of CSCs versus non-exosome-treated CSCs （all P 〈 0.05）. Interestingly, MMPs were downregulated and extracellular matrix （ECM）-related proteins including collagens and fibronectin were upregulated in the exosome-treated CSCs versus non-

关键词： Adipose-derived Mesenchymal Stem Cell Corneal Stromal Cells Exosomes Extracellular Matrix Synthesis

Ginsenoside Rd improves behavioral impairment of rats with acute plateau status by modulating synaptic plasticity

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Chinese Herbal Medicines 2019年第4期11卷 438-441页

作者： hong-bo luo Yun Li Xiang-qun Shi Chun-sheng Xi Department of Neurology The Fifth Affiliated Hospital of Zunyi Medical UniversityZhuhai519000China Department of Nephrology The Fifth Affiliated Hospital of Zunyi Medical UniversityZhuhai519000China Department of Neurology Lanzhou General Hospital of People Liberation ArmyLanzhou 730050China Department of Nephrology Lanzhou General Hospital of People Liberation ArmyLanzhou 730050China

Objective:To investigate the protective effect of ginsenoside Rd on the improvement of the behavior and synaptic plasticity in rats with acute plateau ***:A total of 60 Wistar rats were randomly divided into the control group,the model group,and the intervention group,with 20 rats in each *** model was established in low-pressure oxygen chamber simulating the plateau,and the intervention group was administered with *** microscope was used to observe synaptic ultrastructure of hippocampal CA1 area,and analyze the structural parameters on the Gray I synaptic *** water maze and Y electric maze experiment were used for behavioral ***:Compared with the control group,the number of electrical stimulation required for rat to avoid was increased in the model group,the latency in the Morris water maze was prolonged,the swimming distance was increased,and the frequency of crossing the platform was *** the electron microscope,the synaptic cleft was increased,the length of the synaptic active area was shorter,the postsynaptic density(PSD)was thinner,the flat synapse was increased,and the concave and perforated types were significantly *** with the model group,the number of electrical stimulation required for rat to avoid was decreased in the intervention group,the latency in the Morris water maze was shortened,the swimming distance was decreased,and the frequency of crossing the platform was *** the electron microscope,the synaptic cleft was decreased,PSD was thicker,the flat synapse was decreased,and the concave and perforated types were ***:Low pressure and low oxygen environment of plateau damages the plasticity changes of the synaptic structure and *** to a certain extent,ginsenoside Rd reverses Gray I synaptic interface structure parameters,so as to improve the behavior performance of model rats at high altitude condition.

关键词： acute plateau status behavioral science ginsenoside Rd synaptic plasticity

Comorbidity Burden of Dementia:A Hospital-Based Retrospective Study from 2003 to 2012 in Seven Cities in China

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Neuroscience Bulletin 2017年第6期33卷 703-710页

作者： Qing-Hua Wang Xin Wang Xian-Le Bu Yan Lian Yang Xiang hong-bo luo Hai-Qiang Zou Jie Pu Zhong-He Zhou Xiao-Ping Cui Qing-Song Wang Xiang-Qun Shi Wei Han Qiang Wu Hui-Sheng Chen Hang Lin Chang-Yue Gao Li-Li Zhang Zhi-Qiang Xu Meng Zhang Hua-Dong Zhou Yan-Jiang Wang Department of Neurology and Center for Clinical Neuroscience Daping HospitalThird Military Medical UniversityChongqingChina Department of Preventive Medicine Daping HospitalThird Military Medical UniversityChongqingChina Department of Neurology Chengdu General Hospital of the PLAChengduChina Department of Neurology Lanzhou General Hospital of the PLALanzhouChina Department of Neurology Guangzhou General Hospital of the PLAGuangzhouChina Department of Neurology Wuhan General Hospital of the PLAWuhanChina Department of Neurology Shenyang General Hospital of the PLAShenyangChina Department of Neurology Fuzhou General HospitalFujian Medical UniversityFuzhouChina Department of Prevention Medicine Guangzhou General Hospital of the PLAGuangzhouChina

Dementia is increasing dramatically and imposes a huge burden on society. To date, there is a lack of data on the health status of patients with dementia in China. In an attempt to investigate the comorbidity burden of dementia patients in China at the national level, we enrolled 2,938 patients with Alzheimer＇s disease（AD）, vascular dementia（Va D）, or other types of dementia, who were admitted to tertiary hospitals in seven regions of China from January2003 to December 2012. The Charlson Comorbidity Index（CCI） was used to evaluate the comorbidity burden of the patients with dementia. Among these patients, 53.4% had AD, 26.3% had Va D, and 20.3% had other types of dementia. The CCI was 3.0 ± 1.9 for all patients,3.4 ± 1.8 for those with Va D, and 3.0 ± 2.1 for those with AD. The CCI increased with age in all patients, andthe length of hospital stay and daily expenses rose with age and CCI. Males had a higher CCI and a longer stay than females. Moreover, patients admitted in the last 5 years of the study had a higher CCI than those admitted in the first 5 years. We found that the comorbidity burden of patients with dementia is heavy. These findings provide a better understanding of the overall health status of dementia patients, and help to increase the awareness of clinicians and policy-makers to improve medical care for patients.

关键词： Alzheimer’s disease Vascular dementia Prevalence Comorbidity Charlson comorbidity index

PERK Signaling of Unfolded Protein Response Actived in Acute Hypobaric Hypoxia and Effect of Ginsenoside Rb1

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Chinese Herbal Medicines 2016年第1期8卷 39-43页

作者： Yun Li hong-bo luo Xiang-qun Shi Chun-sheng Xi Jian-kui Guo Zhi-qiang Zhang Li Cao Xiao-yan Zhang Zhao Liu Department of Nephrology Lanzhou General Hospital Lanzhou Military Area Command Lanzhou 730050 China Department of Neurology Lanzhou General Hospital Lanzhou Military Area Command Lanzhou 730050 China

Objective To observe pancreatic ER kinase （PERK） signaling of unfolded protein response （UPR）. Methods The rats were divided into control, model, and ginsenoside Rb; （Rbl） groups, and put into hypoxia chamber to establish the acute plateau stress model. The water solutions of Rbl were given to rats in Rb; group for 7 d. After that, The behavior of rats was observed by Y-maze and passive avoidance test and the rats were sacrificed in a batch for detection by Western blotting. Results Hypobaric hypoxia mediated UPR pathway accompanied the activation of protective pathways such as PERK-elF2a-ATF4 and Grp78/Bip pathways. On the other hand, RbT, the extract from herbal medicine ginseng, increased on the expression of PERK, eIF2a, ATF4, and Grp78/Bip in rats. Conclusion The results indicate that PERK-elF2a-ATF4-GRP78 pathway is a potential target for therapeutic applications in high altitude diseases and Rb] can attenuate the injury to memory function caused by hypobaric hypoxia neurotoxicity.

关键词： ginsengoside Rbl hypobaric hypoxia pancreatic ER kinase pathway

简述江西省洞坞矿区详查阶段矿床开采技术条件

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世界有色金属 2016年第11期41卷 131-132页

作者：洪波罗樊昊斌江西省地质矿产开发局赣西北大队江西九江332000

本矿区处详查阶段,矿床属沉积型石灰岩矿床,主要有水泥用石灰岩矿体、有色冶金熔剂用灰岩矿体和熔剂用白云岩矿体,矿体为奥陶系下统仑山组白云岩、白云质灰岩。本文针对矿山水文地质条件、工程地质条件与环境地质质量展开论述,旨在说明... 详细信息

本矿区处详查阶段,矿床属沉积型石灰岩矿床,主要有水泥用石灰岩矿体、有色冶金熔剂用灰岩矿体和熔剂用白云岩矿体,矿体为奥陶系下统仑山组白云岩、白云质灰岩。本文针对矿山水文地质条件、工程地质条件与环境地质质量展开论述,旨在说明本矿区矿床开采技术条件,为后续勘探及开采提供可靠依据。

关键词：沉积型矿床水文地质工程地质环境地质

九江西海某宾馆滑坡变形特征及应急处置措施建议

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世界有色金属 2016年第9期41卷 58-59页

作者：涂丹东朱成子洪波罗江西省地质矿产勘查开发局赣西北大队江西九江332000

结合具体案例,笔者介绍了该滑坡地貌形态、地层岩性、地质构造条件,分析了滑坡的基本特征,从地形地貌条件、岩土体性质、气象水文因素、人为因素等方面分析了滑坡形成机制,并给出了滑坡应急处置措施建议。

关键词：滑坡变形特征成因机制措施建议

Primary parenchymal squamous cell carcinoma of the kidney:A case report

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World Journal of Clinical Cases 2025年第4期13卷 34-40页

作者： Zhi-Hui Zheng bo Shao Chao-Min Xu Ke Wang Jia-Zhu Wen Li-Kang luo Jia-Cheng Guan Department of Ultrasound The Second People’s Hospital of QuzhouQuzhou 324000Zhejiang ProvinceChina Department of Radiology Shulan(Quzhou)HospitalQuzhou 324000Zhejiang ProvinceChina Department of Radiology The Second People’s Hospital of QuzhouQuzhou 324000Zhejiang ProvinceChina Department of Pathology The Second people’s Hospital of QuzhouQuzhou 324000Zhejiang ProvinceChina

BACKGROUND Primary squamous cell carcinoma(SCC)of the renal parenchyma is extremely rare,with only nine cases *** SUMMARY This study reports a 51-year-old man with primary SCC of the renal *** patient was admitted with recurrent dull pain and discomfort in the right lumbar region,which had worsened over 2 weeks,accompanied by painful gross *** antigen(SCCA)levels were elevated,and imaging revealed a renal mass with associated *** patient underwent laparoscopic unilateral nephrectomy and lymph node *** pathology confirmed highly differentiated SCC with necrosis in the right renal parenchyma,with negative renal pelvis and *** pathological stage was *** months after surgery,the tumor recurred with involvement of the liver,right psoas major muscle,and inferior vena *** patient refused chemotherapy and succumbed to the disease 6 months postoperatively due to disease *** We report a case of primary SCC of the renal parenchyma,a rare renal *** clinical symptoms,laboratory tests,and imaging findings are nonspecific,making accurate and timely diagnosis *** to the literature,for patients with renal calculi accompanied by a renal mass,elevated serum SCCA levels,and magnetic resonance imaging showing cystic or cystic-solid masses within the kidney with pseudocapsules and heterogeneous mild enhancement,the possibility of this disease should be considered.

关键词： Renal tumor Renal parenchyma Squamous cell carcinoma Renal calculi Computed tomography Case report

投喂频率对尼罗系吉富罗非鱼幼鱼胃排空、生长性能和体组成的影响

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水产学报 2011年第11期35卷 1677-1683页

作者：孙晓锋冯健陈江虹罗波赵海祥赵华林广西大学水产研究所

通过胃排空试验与养殖试验研究了不同投喂频率对尼罗系吉富罗非鱼幼鱼的胃排空、生长性能以及体组成的影响。在试验开始时,观测尼罗系吉富罗非鱼幼鱼的胃内饲料排空情况,胃排空试验结果表明,胃排空率的最佳描述为平方根函数,胃内饲料在... 详细信息

通过胃排空试验与养殖试验研究了不同投喂频率对尼罗系吉富罗非鱼幼鱼的胃排空、生长性能以及体组成的影响。在试验开始时,观测尼罗系吉富罗非鱼幼鱼的胃内饲料排空情况,胃排空试验结果表明,胃排空率的最佳描述为平方根函数,胃内饲料在饱食投喂后15 h左右完全排空,达到投喂前水平,80%胃排空为9 h,也就是投喂后大约9 h恢复食欲。360尾试验鱼(初始体质量3.72 g)以不同的投喂频率(1 d 4次、1 d 3次、1 d 2次、2 d 4次、2 d 3次、2 d 2次)分组,每组设立3个平行组,随机养殖于18个网箱中,每箱养殖20尾鱼,按饱食量投饲膨化饲料。养殖期为6周。尼罗系吉富罗非鱼幼鱼在投喂频率为1 d 4次、1 d 3次和1 d 2次时特定生长率和饲料效率显著高于投喂频率为2 d 4次、2 d 3次和2 d 2次时(P0.05)。3.7～48.0 g尼罗系吉富罗非鱼幼鱼的适宜投喂频率为1 d 2次,较2 d 2次、2 d 3次和2 d 4次时明显提高了生长速度和饲料效率,较1 d 3次、1 d 4次摄食量显著降低。2种试验结果较为一致。

关键词：尼罗系吉富罗非鱼投喂频率胃排空生长性能体组成

Blended skip entry guidance for low-lifting lunar return vehicles

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Acta Mechanica Sinica 2014年第6期30卷 973-982页

作者： Zong-Fu luo hong-bo Zhang Guo-Jian Tang College of Aerospace Science and Engineering National University of Defense Technology

A skip entry guidance algorithm blending numerical predictor-corrector and nominal trajectory tracking is presented for lunar return *** guidance is decoupled into longitudinal and lateral channels.A piecewise bank-vs-energy magnitude profile and a sign profile are adopted in the skip phase.A magnitude parameter is used to adjust the predicted downrange,and a pseudo-crossrange at the beginning of the final phase is selected as the lateral control *** biases of both channels are nullified by a false position iteration *** on-line estimation and modeling method is introduced to compensate for aerodynamic and atmospheric uncertainties.A nominal trajectory for the final phase is generated based on actual reenter conditions,and the obtained nominal trajectory is tracked by a linear feedback law.A lateral corridor is used to manage the lateral *** proposed guidance algorithm is assessed using three-degree-of-freedom Monte Carlo analyses,and the results show a satisfactory and robust performance under highly stressful dispersions.

关键词： Skip entry guidance Numerical predictorcorrector Low-lifting vehicle Lunar return mission Bank profile