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miR-15b-5p targeting amyloid precursor protein is involved in the anti-amyloid eflect of curcumin in swAPP695-HEK293 cells

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Neural Regeneration Research 2019年第9期14卷 1603-1609页

作者： hong-ying liu Xian Fu You-Fu Li Xian-Liang Li Zhen-Yu Ma ying Zhang Qing-Chun Gao Department of Neurology the Second Affiliated Hospital of Guangzhou Medical University

Curcumin exerts a neuroprotective effect on Alzheimer’s disease;however,it is not known whether microRNAs are involved in this protective *** study was conducted using swAPP695-HEK293 cells as an Alzheimer’s disease cell ***695-HEK293 cells were treated with 0,0.5,1,2,5,and 10μM curcumin for 24 *** changes in miR-15b-5p,miR-19a-3p,miR-195-5p,miR-101-3p,miR-216b-5p,miR-16-5p and miR-185-5p expression were assessed by real-time quantitative polymerase chain *** mRNA and protein levels of amyloid precursor protein,amyloid-β40 and amyloid-β42 were evaluated by quantitative real-time polymerase chain reaction,western blot assays and enzyme-linked immunosorbent ***695-HEK293 cells were transfected with miR-15b-5p mimic,or treated with 1μM curcumin 24 hours before miR-15b-5p inhibitor *** effects of curcumin on amyloid precursor protein,amyloid-β40 and amyloid-β42 levels were evaluated by western blot assays and enzyme-linked immunosorbent *** assays were used to analyze the interaction between miR-15b-5p and the 3′-untranslated region of amyloid precursor *** results show that amyloid precursor protein and amyloid-βexpression were enhanced in swAPP695-HEK293 cells compared with HEK293 parental *** suppressed the expression of amyloid precursor protein and amyloid-βand up-regulated the expression of miR-15b-5p in swAPP695-HEK293 *** addition,we found a negative association of miR-15b-5p expression with amyloid precursor protein and amyloid-βlevels in the curcumin-treated *** assays revealed that miR-15b-5p impaired the luciferase activity of the plasmid harboring the 3′-untranslated region of amyloid precursor *** findings indicate that curcumin down-regulates the expression of amyloid precursor protein and amyloid-βin swAPP695-HEK293 cells,which was partially mediated by miR-15b-5p via targeting of the 3′-untranslated region of amyloid precursor protein.

关键词： nerve regeneration Alzheimer’s disease natural plant drug curcuminoids miRNAs amyloid precursor protein amyloid-β 3′-untranslated region Luciferase assays neurons neural regeneration

Synthesis of 5-substituted benzyl-2,4-diamino pyrimidine derivatives as c-Fms kinase inhibitors

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Chinese Chemical Letters 2010年第11期21卷 1318-1321页

作者： Li Bao Xu Wei Sun hong ying liu Li Li Wang Jun Hai Xiao Xiao hong Yang Song Li School of Pharmaceutical Sciences Jilin University Changchun 130021 China Laboratory of Computer-Aided Drug Design & Discovery Beijing Institute of Pharmacology and Toxicology Beijing 100850 China

A serials of novel 5-substituted benzyl-2,4-diamino pyrimidine derivatives have been synthesized and evaluated as inhibitors of c-Fms kinase by the standard MTT *** results showed that compound 15,5-[3-methoxy-4-（pyridine-3-yl）benzyl]-2,4-diamino pyrimidine,had an IC50 of 1.45μmol/L in inhibiting the proliferation of M-CSF-dependent myeloid leukemia cells in mice （NFS-60）,which was similar with GW2580,a selective inhibitor of c-Fms kinase.

关键词： C-Fms kinase inhibitors Synthesis 2 4-Diamino pyrimidine

Synthesis and antitumor activity of novel salvicine analogues

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Chinese Chemical Letters 2011年第1期22卷 25-28页

作者： Fei Deng Jin Jian Lu hong ying liu Li Ping Lin Jian Ding Jin Sheng Zhang State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai 201203 China College of Life Sciences Northwest A & F University Yangling 712100 China

Systematic structure modification of the side train of the lead compound saprothoquinone provides a series of salvicine analogues, fifteen of them were first reported. Some compounds were demonstrated potent cytotoxicity against tumor cells with the 50% inhibition concentration in the micromolar range. Furthermore some compounds showed potent topoisomerase II inhibitory effects. The preliminary structure-activity relationship of saprorthoquinone analogues was discussed according to their cytotoxicity against three tumor cells.

关键词： Saprorthoquinone Salvicine Antitumor Structural modification

An intelligent electronic capsule system for automated detection of gastrointestinal bleeding

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第12期11卷 937-943页

作者： hong-ying liu Gang WANG Kang WEI Xi-tian PI Lan ZHU Xiao-lin ZHENG Zhi-yu WEN Key Laboratory of Biorheological Science and Technology of Ministry of Education College of Bioengineering Chongqing University Chongqing 400030 China Key Laboratories for National Defense Science and Technology of Innovative Micro-Nano Devices and System Technology Chongqing University Chongqing 400030 China

In clinical practice,examination of the hemorrhagic spot (HS) remains *** this paper,we describe a remote controlled capsule (RCC) micro-system with an automated,color-based sensor to identify and localize the HS of the gastrointestinal (GI) *** vitro testing of the detecting sensor demonstrated that it was capable of discriminating mimetic intestinal fluid (MIF) with and without the hemoglobin (Hb) when the concentration of Hb in MIF was above 0.05 g/***,this RCC system is able to detect the relatively accurate location of the HS in the GI tract.

关键词： Smart capsule Intestinal bleeding Color sensor Biomedical micro-electromechanical system (BioMEMS)

Synthesis of 1-aryl-3-(3,4-dihydro-2H-chromen-5-yl) ureas as TNF-αinhibitors

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Chinese Chemical Letters 2007年第8期18卷 905-908页

作者： Xin Ming Zhou Zhi Bing Zheng hong ying liu Wu Zhong Jun Hai Xiao Li Li Wang Song Li Institute of Pharmacology and Toxicology Academy of Military Medical Sciences Beijing 100850 China School of Pharmaceutical Engineering Shenyang Pharmaceutical University Shenyang 110016 China

A new series of compounds, 1-aryl-3-（3,4-dihydro-2H-chromen-5-yl） ureas, have been synthesized and their structures were confirmed by FAB-MS and IH NMR. The preliminary pharmacological screening showed that these co... 详细信息

关键词： p38 MAPK inhibitor l-Aryl-3-(3,4-dihydro-2H-chromen-5-yl) ureas Synthesis

Hydrogen peroxide is a risk factor for occupational chemical poisoning

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Chinese Medical Journal 2021年第7期134卷 881-882页

作者： Xiao-Yan Yang hong-ying liu Departm ent of Occupational Health Ankang Center for Disease Control and PreventionAnkangShaanxi 725000China Department of Occupational Health Baoji Center for Disease Control and PreventionBaojiShaanxi 721000China

To the Editor:Hydrogen peroxide is a strong oxidant,which is commonly used as a disinfectant mainly in medical and chemical *** peroxide poisoning causes brain infarction,and ingestion of hydrogen peroxide injures the upper gastrointestinal tract.[1]Besides,food poisoning is often caused due to hydrogen peroxide.A case has been reported in China that cerebral embolism was caused by oral hydrogen peroxide poisoning.[2]The first two cases of hydrogen peroxide poisoning caused by sterilization and disinfection in China are illustrated in this article.

关键词： hydrogen peroxide chemical

Surgical Resection of Sternal Tumors and Reconstruction with Titanium Mesh

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Chinese Medical Sciences Journal 2011年第4期26卷 237-240页

作者： hong-sheng liu ying-zhi Qin Shan-qing Li Li Li Yu-shang Cui Zhi-yong Zhang Department of Thoracic Surgery Peking Union Medical College Hospital ChineseAcademy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China

Objective To evaluate the use of titanium mesh reconstruction after sternal tumor resection. Methods From January 2007 to January 2011, 14 patients with sternal tumors were admitted into Peking Union Medical Hospital. The clinical characteristics, surgical resection, and technique of reconstruction were reviewed. Results Of the 14 patients, 3 had a metastatic sternal tumor, the primary sites of which were as follows: hepatic carcinoma in one case (metastasis 19 years after operation), breast carcinoma in another case (metastasis 5 years after operation), and renal carcinoma in the other case (found simultaneously). Two patients showed local involvement of the sternum: 1 had thymic carcinoma, and the other had myofibrosarcoma. The remaining 9 patients had primary tumors: 4 were osteochondroma, 3 chondrosarcoma, 1 eosinophilic granuloma, 1 non-Hodgekin's lymphoma. En bloc resection of the sternal tumor was performed in all the 14 patients. The defect was repaired with the titanium mesh adjusted to the shape of the defect and fixed with the stainless steel wire. Eleven patients were followed up for a period from 2 months to 4 years, during which no translocation or broken of the titanium mesh was observed. Conclusions Radical en bloc excision remains the treatment of choice for sternal tumors. Sternum defect reconstruction using titanium mesh as a rigid replacement proves appropriate and effective.

关键词： sternal tumor en bloc resection sternal reconstruction rigid prosthetic replacement titanium mesh

On the Politeness in College English Teachers＇ Classroom Talk

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Sino-US English Teaching 2015年第3期12卷 159-163页

作者： XI hong-mei SONG hong QU ying liu Dan Harbin Engineering University Harbin China

Teachers＇ talk is an important aspect for conducting college English class effectively. It plays crucial roles in giving instructions, organizing classroom norm, and facilitating students＇ language acquisition. Based on Leech＇s Politeness Principle, this paper has analyzed the politeness in teachers＇ classroom talk and explored the functions it possesses as well. The study shows that the politeness feature of college English teachers＇ classroom talk is the consequences of observing each maxim of the Politeness Principle and is of the functions such as making effective cornmnication, keeping friendly relations with students, saving students＇ face, etc.. Conducting research on politeness in teachers＇ talk is conducive to understanding the process of language teaching and learning.

关键词： eachers' talk politeness Politeness Principle

Inhibition effect of small interfering RNA of connective tissue growth factor on the expression of vascular endothelial growth factor and connective tissue growth factor in cultured human peritoneal mesothelial cells

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Chinese Medical Journal 2007年第3期120卷 231-236页

作者： liu Fu-you XIAO Li PENG You-ming DUAN Shao-bin liu hong liu ying-hong LING Gui-hui YUAN Fang CHEN Jun-xiang FU Xiao ZHU Jian-lian Division of Nephrology Second Affiliated Xiangya Hospital Central South University Changsha 410002 China

Background The peritoneum response to peritoneal dialysis can lead to fibrosis. The transforming growth factor β1 （TGF-β1 ） plays a key role in regulating tissue repair and remodelling after injury. Connective tissue growth factor （CTGF）, a downstream mediator of TGF-β1 inducing fibrosis, has been implicated in peritoneal fibrosis. Vascular endothelial growth factor （VEGF） plays a key role in angiogenesis that can hasten peritoneal fibrosis. In this study, we investigated the effect of small interfering RNA （siRNA） of CTGF by pRETRO-SUPER （PRS） retrovirus vector on the expression of CTGF and VEGF in human peritoneal mesothelial cells. Methods Retrovirus producing CTGF siRNA were constructed from the inverted oligonucleotides and transferred into packaging cell line PT67 with lipofectamine, and the virus supernatant was used to infect human peritoneal mesothelial cell （HPMC）. The cells were divided into seven groups： low glucose DMEM, low glucose DMEM ＋ TGF-β1 5 ng/ml, low glucose DMEM ＋ TGF-β1 5 ng/ml ＋ PRS-CTGF-siRNA1-4 and low glucose DMEM ＋ TGF-β1 5 ng/ml ＋ PRS. The expression of CTGF and VEGF were measured by semiquantitative RT-PCR and Western blot. Results Low levels of CTGF and VEGF were detected in confluent HPMCs. Following stimulation with TGF-β1 , the levels of CTGF and VEGF were significantly upregulated （P〈0.01）. Introduction of PRS-CTGF-siRNA1-4 resulted in the significant reduction of CTGF mRNA and protein, and VEGF mRNA （P〈0.01）, especially in groups PRS-CTGF-siRNA, and PRS-CTGF-siRNA4. The introduction of PRS void vector did not have these effects （P〉0.05）. Conclusions The expression of CTGF siRNA mediated by PRS retrovirus vector can effectively reduce the level of CTGF and VEGF induced by TGF-β1 in cultured HPMCs. This study may provide potential therapeutic strategies to prevent the peritoneal fibrosis.

关键词： peritonealfibrosis connective tissue growth factor vascular endothelial growth factor,RNA, small interfering