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Alisol B acetate induces apoptosis of SGC7901 cells via mitochondrial and phosphatidylinositol 3-kinases/Akt signaling pathways

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World Journal of Gastroenterology 2009年第23期15卷 2870-2877页

作者： Yong-hong Xu li-jie Zhao Yan li Department of DigestiveDiseases Shengjing Hospital of China Medical UniversityShenyang 110004 Liaoning Province China

AIM： To examine the effect of alisol B acetate on the growth of human gastric cancer cell line SGC7901 and its possible mechanism of action.METHODS： The cytotoxic effect of alisol B acetate on SGC7901 cells was measured by 3-（4,5-dimethylthiazol- 2-yl）-2,5-diphenyltetrazolium bromide （MI-I-） assay. Phase-contrast and electron microscopy were used to observe the morphological changes. Cell cycle and mitochondrial transmembrane potential （A^Pm） were determined by flow cytometry. Western blotting was used to detect the expression of apoptosis-regulated gene Bcl-2, Bax, Apaf-1, caspase-3, caspase-9, Akt, P-Akt and phosphatidylinositol 3-kinases （PI3K）.RESULTS： Alisol B acetate inhibited the proliferation of SGC7901 cell line in a time- and dose-dependent manner. PI staining showed that alisol B acetate can change the cell cycle distribution of SGC7901, increase the proportion of cells in G0-G1 phase and decrease the proportion of S phase cells and G2-M phase cells. Alisol B acetate at a concentration of 30 pmol/L induced apoptosis after 24, 48 and 72 h incubation, with occurrence rates of apoptotic cells of 4.36%, 14.42% and 21.16%, respectively. Phase-contrast and electron microscopy revealed that the nuclear fragmentation and chromosomal condensed, cells shrank and attachment loss appeared in the SGC7901 treated with alisol B acetate. Apoptosis of SGC7901 cells was associated with cell cycle arrest, caspase-3 and caspase-9 activation, loss of mitochondrial membrane potential and up-regulation of the ratio of Bax/Bcl-2 and inhibition of the PI3K/Akt.CONCLUSION： Alisol B acetate exhibits an antiproliferative effect in SGC7901 cells by inducing apoptosis. Apoptosis of SGC7901 cells involves mitochondria-caspase and PI3K/Akt dependent pathways.

关键词： Alisol B acetate Apoptosis Mitochondria Phosphatidylinositol 3-kinases/Akt SGC7901 cells

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Iodine-mediated regioselective hydroxyselenenylation of alkenes:Facile access to β-hydroxy selenides

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Chinese Chemical Letters 2018年第3期29卷 479-481页

作者： Xian-Long Wang hong-jie li jie Yan College of Chemical Engineering Zhejiang University of Technology

In the presence of molecular iodine, the reaction of alkenes with diselenides proceeds efficiently under air and at room temperature in mixed solvent MeCN/H2O, which affording β-hydroxy selenides with high regioselectivity and in good to excellent yields. This iodine-mediated vicinal difunctionalization of alkenes requires mild reaction conditions and is a simple procedure, which extends the synthetic application of molecular iodine in organic synthesis.

关键词： β-Hydroxy selenide Hydroxyselenenylation Diselenide Alkene I2

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Introgression of Resistance to Powdery Mildew Conferred by Chromosome 2R by Crossing Wheat Nullisomic 2D with Rye

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Journal of Integrative Plant Biology 2006年第7期48卷 838-847页

作者： Diao-Guo An li-Hui li Jun-Ming li hong-jie li Yong-Guan Zhu Centre of Agricultural Resources Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Shijiazhuang 050021 China Institute of Crop Sciences the Chinese Academy of Agricultural Sciences Beijing 100081 China Research Center for Eco-environmental Sciences the Chinese Academy of Sciences Beijing 100085 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

Using the nulUsomic back-cross procedure, four wheat-rye chromosome substitution 2R （2D） lines with different agronomic performance, designated WR02-145-1, WR01-145-2, WR02-145-3, and WR02-145-4, were produced from a cross between 2D nullisomic wheat （Triticum aestivum L. cv. ＂Xiaoyan 6＂） and rye （Secale cereale L. cv. ＂German White＂）. The chromosomal constitution of 2n=42=21 in WR02-145 lines was confirmed by cytological and molecular cytogenetic methods. Using genomic in situ hybridization on root tip chromosome preparations, a pair of intact rye chromosomes was detected in the WR02-145 lines. PCR using chromosome-specific primers confirmed the presence of 2R chromosomes of rye in these wheat-rye lines, indicating that WR02o145 lines are disomic chromosome substitution lines 2R （2D）. The WR02-145 lines are resistant to the powdery mildew （Erysiphe graminis DC. f. sp. tritici E. Marchal） isolates prevalent in northern China and may possess gene（s） for resistance to powdery mildew, which differ from the previously identified Pm7gene located on chromosome 2RL. The newly developed ＂Xiaoyan 6＂- ＂German White＂ 2R （2D） chromosome substitution lines are genetically stable, show desirable agronomic traits, and are expected to be useful in wheat improvement.

关键词： genomic in situ hybridization nullisomic back-cross procedure PCR powdery mildew resistance wheat-rye chromosome substitution line

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ZM-66, a New Podophyllotoxin Derivative Inhibits Proliferation and Induces Apoptosis in K562/ADM Cells

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Chinese Medical Sciences Journal 2014年第3期29卷 174-179页

作者： ling li hong-jie li Jian-sheng zhi hong Chen Wen-li Xie Department of Pharmacology Logistics University of Chinese People's Armed Police Forces Department of Oral Pathology Stomatological Hospital Tianjin Medical University Department of Anatomy Tianjin Medical University

Objective To investigate the anti-tumor effect of ZM-66 on multidrug-resistant leukemic cell line K562/ADM. Methods The K562/ADM cells were treated with varying concentrations （0, 1, 2, 4×10^-3 mmol/L） of ZM-66 or etoposide for 24 hours. The proliferation was detected by Sulforhodamine B Sodium Salt （SRB） assay and apoptosis was detected by flow cytometry analysis and fluorescent staining. In addition, the expression levels of p53 and bax genes in K562/ADM cells were detected by RT-PCR analysis. The level of P-glycoprotein （P-gp）, P53 and Bax protein in K562/ADM cells were detected by Western blot assay. Results SRB assay demonstrated that etoposide had little inhibitory effect on K562/ADM cells, whereas ZM-66 （1, 2, 4×10^-3 mmol/L） had significantly inhibitory effect on K562/ADM cells （all P〈0.01）. The acridine orange/propidium iodide dual staining showed that there were typical condensation of chromatin and nuclear fragmentation nuclei with red color in ZM-66 treated cells. Flow cytometric analysis showed that there was a significantly increase of apoptotic cells i~ K562/KDM cells after treated with ZM-66. RT-PCR showed that the p53 and bax mRNA expression levels in K562/ADM cells treated with ZM-66 at 1, 2, 4×10^-3 mmol/L were higher than those in the cell without treatment. Western blot showed that the P53 and Bax protein expression levels in K562/ADM cells treated with ZM-66 at 2, 4x 10 s mmol/L were higher than those in the cell without treatment. But the P-gp protein expression level in K562/ADM cells treated with ZM-66 at 2, 4×10^-3 mmol/L was gradually lower than those in the cell without treatment. Conclusion ZM-66 is able to induce cell death by apoptosis in vitro, as a result of the reverse of theapoptosis resistance in drug-resistant K562/ADM cells by modulating expression of key factors associated with apoptosis induction.

关键词： ZM-66 podophyllotoxin multidrug resistance P-glycoprotein apoptosis

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A Study of Elemental Abundance Pattern of the r-II Star HD 222925

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Research in Astronomy and Astrophysics 2023年第12期23卷 118-127页

作者： Fang Wen Wan-Qiang Han Wen-Yuan Cui hong-jie li Bo Zhang College of Physics Hebei Normal UniversityShijiazhuang 050024China Department of Physics Shijiazhuang UniversityShijiazhuang 050035China School of Sciences Hebei University of Science and TechnologyShijiazhuang 050018China

HD 222925 is a rare r-process enhanced star in the Milky Way because of its metal abundance([Fe/H]=-1.46±0.10)and Eu abundance([Eu/Fe]=1.32±0.08).Based on the very complete set of elemental abundances of HD 222925,we use the abundance decomposition method to fit the observed abundances of 58elements in the sample star,which is also the largest number of elemental abundances fitted at the same time for a fixed *** analyze the astrophysical origins of elements in HD 222925 by its abundance ratios and component *** is found that the light elements and the iron group elements in HD 222925 mainly originate from the primary process of the TypeⅡsupernovae(SNeⅡ)with the progenitor mass M>10 M_(⊙)and have no contribution from SNe Ia and the first generation of very massive *** contribution of the weak r-process to Ga,Ge,and As is superior to that of the other processes,and its contribution decreases linearly with increasing atomic *** main r-process that is likely derived from a neutron star merger plays a key role in the formation of neutroncapture elements(Z≥38)in HD 222925.

关键词： stars chemically peculiar-stars individual(HD 222925)-nuclear reactions-nucleosynthesis-abundances-stars abundances

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Nucleosynthesis in Advection-Dominated Accretion Flow onto a Black Hole

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Chinese Physics Letters 2017年第4期34卷 128-131页

作者： Jiang Zhang Ren-Yi Ma hong-jie li Bo Zhang Department of Mathematics and Science Hebei GEO University Shijiazhuang 050016 Key Laboratory for the Structure and Evolution of Celestial Objects Chinese Academy of Sciences Kunming 650011 Department of Astronomy Xiamen University Xiamen 361005 School of Sciences Hebei University of Science and Technology Shijiazhuang 050016 Department of Physics Hebei Normal University Shijiazhuang 050016

Nucleosynthesis in advection-dominated accretion flow （ADAF） onto a black hole is proposed to be an important role in chemical evolution around compact stars. We investigate the nucleosynthesis in ADAF relevant for a black hole of low mass, different from that of the self-similar solution. In particular, the presence of supersolar metal mass fractions of some isotopes seems to be associated with the known black hole nucleosynthesis in ADAF, which offers further evidence of diversity of the chemical enrichment.

关键词： Nucleosynthesis in Advection-Dominated Accretion Flow onto a Black Hole

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Understanding and modifications on lithium deposition in lithium metal batteries

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Rare Metals 2022年第8期41卷 2800-2818页

作者： Qin-Ya Yang Zhao Yu Yao li Wang Zhang Hao-Wen Yuan hong-jie li Wei Ma Shen-Min Zhu Sa li State Key Laboratory of Metal Matrix Composites School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai200240China School of Materials Science and Engineering Tongji UniversityShanghai201804China

lithium metal has been considered as an ultimate anode choice for next-generation secondary batteries due to its low density,superhigh theoretical specific capacity and the lowest voltage ***,uncontrollable dendrite growth and consequently large volume change during stripping/plating cycles can cause unsatisfied operation efficiency and serious safety concern,restraining its commercial use for the last 40 *** widely used lithium-ion battery is approaching its theoretical limit at present,it is increasingly urgent to develop new energy storage equipment with sufficient practical ***,two important processes of lithium deposition,nucleation and growth on lithium metal anode are *** three-dimensional(3 D)porous anodes with unique structure and dynamic surface conditions,as one of the most efficient designs on dendrites suppression,are specifically discussed in terms of their beneficial adjustment on the dendrite *** on current designs and prospects on future advanced porous anode are explored in the end.

关键词： lithium metal batteries lithium nucleation Dendrites growth Porous anodes

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Potential Hazards in Smoke-Flavored Fish

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Journal of Ocean University of China 2008年第3期7卷 294-298页

作者： liN hong JIANG jie li Donghua Seafood Safety Lab College of Food Science and Engineering Ocean University of China Qingdao 266003 P. R. China

Smoking is widely used in fish processing for the color and flavor. Smoke flavorings have evolved as a successful alternative to traditional smoking. The hazards of the fish products treated by liquid-smoking process are discussed in this review. The smoke flavoring is one important ingredient in the smoke-flavored fish. This paper gives the definition of smoke flavorings and the hazard of polycyclic aromatic hydrocarbons （PAHs） residue in the smoke flavorings on the market. It gives also an assessment of chemical hazards such as carcinogenic PAHs, especially Benzo-[α]pyrene, as well as biological hazards such as listeria monocytogenes, Clostridium botulinum, histamine and parasites in smoke-flavored fish. The limitations in regulations or standards are discussed. Smoke flavored fish have lower content of PAHs as compared with the traditional smoking techniques if the PAHs residue in smoke flavorings is controlled by regulations or standards.

关键词： smoke-flavored fish hazards smoke flavoring PAHs

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Research on stability of a slope due to underground mining

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Journal of Coal Science & Engineering(China) 2013年第4期19卷 474-482页

作者： hong-jie li Hui-Ya Zhong Wei-Chao li Mine Safety Technology Branch China Coal Research Institute Beijing 100013 China State Key Laboratory of Coal Mining and Clean Utilization (China Coal Research Institute) Beijing 100013 China Mid-South Design and Research Institute CHEC Changsha 410014 China China Institute of Water Resources and Hydropower Research Beijing 100048 China

This paper will present a detailed analysis of the deformation mechanism and stability assessment of the slope through field investigations, numerical modeling and measurements. Field investigation indicated that three thin coal seams encountered large mined-out area at one side and free surface of hill slope at the other side, which lead to the caving of roof strata movement, ground movement and crown crack along the preferred orientations of joints. The three-dimensional numeri- cal modeling study on the case demonstrated that the plasticity failure occurred gradually along with the extension of mined-out area in depth. When the depth of mining reached the verge defined by the seismic prospecting method, a large mount of tension failure occurred on the crown of the slope. The factor of safety was 1.36 calculated by the shear strength reduction technique, which indicated the slope was in stable state. The measurement showed that the residual deformation occurred before 1998 and became stable subsequently, which indicated that the residual deformation almost finished and the slope is in stable state.

关键词： underground mining stability of slope numerical modeling strength reduction technique monitoring

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Expression of IL-26 predicts prognosis of patients with hepatocellular carcinoma after surgical resection

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Hepatobiliary & Pancreatic Diseases International 2019年第3期18卷 242-248页

作者： Zhi-Feng Xi Seogsong Jeong Chen-Chen Wang hong-jie li Han Guo jie Cai Jia-Xin li Xiao-Ni Kong Ying Tong Qiang Xia Department of liver Surgery Renji Hospital School of Medicine Shanghai Jiao Tong University

Background: There is no data regarding prognostic impact of interleukin(IL)-26 on outcomes of patients with hepatocellular carcinoma(HCC). The present study aimed to evaluate the prognostic impact of IL-26 on HCC patients undergoing liver resection. Methods: From 2003 to 2008, 122 patients with HCC who received surgical curative resection were enrolled. Patients were stratified into IL-26-upper and-lower groups according to the median expression level from immunohistochemical staining of resected specimens. Prognostic impact of IL-26 was estimated using Kaplan–Meier curves. Univariate and multivariate analyses were performed to evaluate timedependent prognostic impact and independency of IL-26. Demographic and clinical factors that were associated with IL-26 were comprehensively identified. Results: Prognosis of the patients with high level of IL-26 revealed to be significantly unfavorable in both cumulative recurrence-free survival( P linical therapy when planning individualized follow-up schedule and evaluating candidates for prophylactic adjuvant treatment to prevent recurrence.

关键词： Interleukin 26 Hepatocellular carcinoma Surgical resection Prognostic factors

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