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“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： hong-fei xia,tian-zhu he,yi cui,xu ma reproductive and genetic center of national research institute for family planning,beijing,100081

Background:MicroRNAs(miRNAs) are small noncoding RNAs whose function as modulators of gene expression is crucial for the proper control of cell *** many microRNAs were found to express in central nervous system(CNS),the role of the regulatory networks in which they are involved and their function in the pathological process of nerve cells are only just *** the present study,the possible mechanisms by which one neuronal miRNAs,miR-125b,affected the growth of nervous cells were investigated using in vitro cell line ***:The expression pattern of miR-125b in ATRA-treated human glioma cell lines was detected by Northern blotting and in situ *** effect of miR-125b on the proliferation and apoptosis of human glioma cells was analyzed by MTS assay,TUNEL and Flow cytometry *** addition,the identification of target gene of miR-125b was studied by dualluciferase activity assay and Immunoblot ***:We found differential expression of miR-125b in 1.0 uM all-trans-retinoic acid(ATRA)-treated human glioma cell ***-regulation of miR-125b partially restored cell viability and inhibited cell apoptosis in U343 cells treated by ***-regulation of miR-125b decreased human glioma cells proliferation and enhanced the sensitivity of human glioma cells to ATRA-induced *** addition,we found an inverse relationship between the expression of miR-125b and the cell apoptosis-related protein Bcl-2 modifying factor(Bmf),and miR-125b can interact with 3c-untranslated region(UTR) of ***:These findings indicate that overexpression of miR-l25b promotes human glioma cell proliferation and inhibits ATRA-induced cell apoptosis and low expression of miR-125b sensitizes cells to ATRAinduced *** may play an important role in the process of miR- 125b influencing cell apoptosis.

关键词： miR-125b Human glioma cells Proliferation Apoptosis Bmf

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A preliminary investigation on the role of miR-185 in breast cancer

A preliminary investigation on the role of miR-185 in breast...

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“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Rui Wang~1,hong-fei xia~2 and xu ma~2 1.Graduate School of Peking Union Medical College,beijing,100005 2.reproductive and genetic center of national research institute for family planning,beijing,100081

MicroRNA are endogenously small non-coding RNAs which regulate gene expression by blocking the translation or decreasing the stability of ***-185 is one of this family members,which can induce cell cycle arrest in human non small cell lung cancer and suppress tumor ***’s still unclear whether it has the relationship to breast cancer or *** this study,we found that miR-185 inhibited cellular proliferation and promoted apoptosis in T47D cells,indicating that miR-185 might inhibit the occurrence of breast *** futher explore the possible mechanisms in this process,we analysed its target genes. Targetscan and PicTar algorithms showed that stathmin 1(STMN1) may be one of miR-185 target genes. STMN1 is a microtubule-associated protein involved in the regulation of the microtubule filament system by destabilizing microtubules and takes part in the preventative progression of ER-positive primary in breast *** validate STMN1 as the target of miR-185,we set up a luciferase reporter assay.3’-UTR segment of STMN1 was amplified by PCR from human cDNA and inserted downstream of the termination codon of the luciferase reporter gene in the pGL3 control vector.3’-UTR segment of STMN1,deleting putative miR-185 biding site,was used as a negative *** sense reporter vector was respectively co-transfected with miR-185 mimics,mimics negative control,miR-185 inhibitors,or inhibitor negative control into T47D *** results shows that luciferase activity was not affected neither miR-185 mimics nor miR-185 inhibitors,which indicates that STMN1 may not be the target gene of *** further identify this results,the sense or control reporter vector was co-transfected with miR-185 ***, the luciferase activity wasn’t changed *** results indicate that miR-185 may have no association with ***,our data collectively suggest that miR-185 may be involved in the preventative progression of bresast cancer,not related with STMN1.

关键词： miR-185 breast cancer STMN1 target genes

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miR-128真核表达载体的构建及功能的初步研究

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生殖医学杂志 2010年第2期19卷 146-151页

作者：夏红飞贺天柱崔熠马旭国家人口计生委科学技术研究所遗传优生中心北京100080 中国农业大学生物学院农业生物技术国家重点实验室北京100093

目的构建miR-128真核表达载体,研究其在神经胶质瘤细胞株U343表达及对U343细胞增殖的影响。方法以人神经胶质瘤细胞株U343的DNA为模板扩增miR-128-1和miR-128-2的前体序列,将人miR-128-1(hsamiR128-1)和人miR-128-2(hsa-miR128-2)基因克隆到真核表达载体pCR3.1,将pCR3.1-miR-128-1(p-128-1)和pCR3.1-miR-128-2(p-128-2)表达载体瞬时转染U343细胞,采用Northern印迹检测成熟的miR-128表达。将U343细胞接种96孔板,MTT法检测过表达miR-128对细胞增殖的影响。结果p-128-1和p-128-2表达载体经酶切及测序鉴定正确;二者转染细胞后,反转录-聚合酶链反应(RT-PCR)扩增结果显示,其能有效表达miR-128的前体;Northern印迹结果均能产生成熟态的miR-128。MTT检测结果在无血清培养和维甲酸处理的情况下,细胞的增殖能力明显下降。结论成功构建了p-128-1和p-128-2的真核表达载体,转染神经胶质瘤细胞后能有效表达,miR-128基因过表达能抑制U343细胞的增殖。

关键词： miR-128 真核表达载体 U343细胞细胞增殖基因表达

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Two common SNPs in pri-miR-125a alter the mature miRNA expression and associate with recurrent pregnancy loss in a Han-Chinese population

Two common SNPs in pri-miR-125a alter the mature miRNA expre...

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“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： yi Hu~(1,2),Chun-Mei Liu~(1§),Lu Qi~(1,2),tian-zhu he~3,Liu Shi-Guo~4,Chan-Juan Hao~(1,2),yi cui~(1,2), Ning Zhang~(1,2),hong-fei xia~(1,2),xu ma~(1,2) 1.reproductive and genetic center of national research institute for family planning,beijing,100081,China 2.Graduate School,Peking Union Medical College,beijing 3.State Key Laboratory of Agrobiotechnology,College of Biological Sciences,China Agricultural University,beijing,100094,China 4.Affiliated hospital of medical college of Qingdao university,Qingdao,266011,China

Although there are plenty of evidence that single-nucleotide polymorphisms(SNPs) that fall within coding sequences of genes are involved in recurrent pregnancy loss(RPL),it is still unknown whether the polymorphisms in microRNAs(miRNAs) are related with *** uiis study,we established this kind of association by confirming significant differences in genotype distribution of rs41275794(P= 0.0005) and rs12976445(P= 0.001) within the pri-miR-125a in 217 Han Chinese patients of RPL compared with 431 *** on this observation,two-locus haplotypes were constructed and the A-T haplotype was found to be associated with an increased risk of RPL(OR=2.84,95%C.I.1.98-4.07,/M).0000000057). Further analysis showed that the levels of pre- and mature- miR-125a were down-regulated in the cells transfected with the A-T haplotype,which was consistent with in vivo detection that the level of mature miR-125a was lower in 30 pregnant women with A-T haplotype than that with G-C *** in vitro RNA processing assays,we found a similar decrease in the amount of pre-miR-125a and decline in binding capacity of nuclear factors to pri-miR-125a with A-T *** importantly,the reduction in miR-125a,as a consequence of A-T haplotype,further led to less efficient inhibition of target genes,LIFR and ERBB2,which play important roles in the embryo implantation and ***,our data collectively suggest that two common polymorphisms in pre-miR-125a might contribute to the genetic predisposition to RPL by disrupting the production of miR-125a,which consequently interfered in the expression and function of target genes of miR-125a.

关键词： polymorphism recurrent pregnancy loss microRNA haplotype pre-miRNA pri-miRNA

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Comparative metagenomic analysis of the vaginal microbiome in healthy women

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Synthetic and Systems Biotechnology 2021年第2期6卷 77-84页

作者： fei Liu yingshun Zhou Lisi zhu Zhiyi Wang LiangKun ma Yuan he Pengcheng Fu CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing100101China Department of Pathogenic Biology Pathogenic Biology platformSchool of Basic Medical SciencesSouthwest Medical UniversityLuzhou646000Sichuan ProvinceChina Laboratory of Clinical genetics Medical Research CenterState Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijingChina CAS Key Laboratory of Microbial Physiological and Metabolic Engineering Institute of MicrobiologyChinese Academy of SciencesBeijing100101China Peking Union Medical College Hospital(Dongdan Campus) No.1 Shuai Fu YuanBeijing100730China national Human genetic Resources center National Research Institute for Health and Family PlanningBeijingChina Mybiome(beijing)Medical Consulting Co.LTD China

The composition of these vaginal microbiome has a significant impact on women’s ***,few studies have characterized the vaginal microbiome of healthy Chinese women using metagenomic ***,we carried out a comparative metagenomic analysis to survey taxonomic,functional levels,and microbial communities’genome content in healthy women’s vaginal ***,we observed a total of 111 species,including all dominant vaginal Lactobacillus species,such as ***,***,***,and *** microbial taxa,several pathways were ubiquitous and prevalent across individuals,including adenosine ribonucleotides de novo biosynthesis and pyruvate fermentation to acetate and lactate Ⅱ.Notably,our diversity analysis confirmed a significant difference in healthy women from different ethnic ***,we binned vaginal assemblies into 62 high-quality genomes,including 9 ***,7 ***,5 ***,and 5 *** identified the pan and core genomes of *** and *** and revealed the genetic *** differences between strains were the hypothetical genes and mobile element-like *** results provide a framework for understanding the implications of the female reproductive tract’s composition and functional potential and highlight the importance of genome-resolved metagenomic analysis to further understand the human vaginal microbiome.

关键词： Vaginal microbiome Metagenome Metabolic pathways Genome Strains

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genetically engineered FGF1-sericin hydrogel material treats intrauterine adhesion and restores fertility in rat

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Regenerative Biomaterials 2022年第1期9卷 67-80页

作者： Chun-yi Guan Feng Wang Lu Zhang xue-Cheng Sun Dan Zhang Hu Wang hong-fei xia Qing-You xia xu ma reproductive and genetic center of national research institute for family planning Beijing 100081People’s Republic of China Graduate School Peking Union Medical CollegeBeijing 100005People’s Republic of China Biological Science research center Chongqing Key Laboratory of Sericultural ScienceChongqing Engineering and Technology Research Center for Novel Silk MaterialsSouthwest UniversityChongqing 400715People’s Republic of China

Endometrial injury can cause intrauterine adhesions(IUA)and induce the formation of endometrial fibrosis,leading to infertility and *** present,there is no effective treatment method for severe IUA and uterine basal injury with adhesion area larger than one-third of the *** this study,we prepared FGF1 silk sericin hydrogel material(FGF1-SS hydrogel)to treat endometrial injury and prevent endometrial *** with the silk sericin hydrogel material(WT-SS hydrogel),FGF1-SS hydrogel significantly promotes the cell migration and infiltration ability of endometrial stromal cells(ESCs).More importantly,FGF1-SS hydrogel can release FGF1 stably for a long time and inhibit the ESCs injury model forms fibrosis through the TGF-β/Smad *** the IUA rat model,FGF1-SS hydrogel treatment effectively restored the number of uterine glands and uterine wall thickness in rats,with a fertility rate of 65.1%66.4%.The results show that FGF1-SS hydrogel is expected to be a candidate to prevent IUA.

关键词： intrauterine adhesions FGF1-sericin hydrogel fibrosis TGF-β/Smad

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Comparative genomic and transcriptomic analyses uncover the molecular basis of high nitrogen-use efficiency in the wheat cultivar Kenong 9204

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Molecular Plant 2022年第9期15卷 1440-1456页

作者： xiaoli Shi Fa cui Xinyin Han yilin he Long Zhao Na Zhang Hao Zhang Haidong zhu Zhexin Liu Bin ma Shusong Zheng Wei Zhang Jiajia Liu xiaoli Fan Yaoqi Si Shuiquan tian Jianqing Niu Huilan Wu xuemei Liu zhuo Chen Deyuan Meng xiaoyan Wang Liqiang Song Lijing Sun Jie Han Hui Zhao Jun Ji Zhiguo Wang xiaoyu he Ruilin Li xuebin Chi Chengzhi Liang Beifang Niu Jun xiao Junming Li hong-Qing Ling State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Key Laboratory of Molecular Module-Based Breeding of High yield and Abiotic Resistant Plants in Universities of Shandong College of AgricultureLudong UniversityYantai 264025China Computer Network Information center Chinese Academy of SciencesBeijing 100190China center for Agricultural Resources research Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050022China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China School of Computer Science and Technology University of Chinese Academy of SciencesBeijing 100049China CAS-JIC Centre of Excellence for Plant and Microbial Science Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Yazhou Bay Seed Laboratory of Hainan Province Sanya 572019China

Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental *** this study,we generated a high-quality genome assembly for the high-NUE wheat cultivar Kenong 9204 and systematically analyzed genes related to nitrogen uptake and *** comparative analyses,we found that the high-affinity nitrate transporter gene family had expanded in *** studies showed that subsequent functional differentiation endowed the expanded family members with saline inducibility,providing a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various *** explore the genetic and molecular mechanisms of high NUE,we compared genomic and transcriptomic data from the high-NUE cultivar Kenong 9204(KN9204)and the low-NUE cultivar Jing 411 and quantified their nitrogen accumulation under high-and low-nitrogen *** with Jing 411,KN9204 absorbed significantly more nitrogen at the reproductive stage after shooting and accumulated it in the shoots and *** data analysis revealed that nitrogen deficiency clearly suppressed the expression of genes related to cell division in the young spike of Jing 411,whereas this suppression of gene expression was much lower in *** addition,KN9204 maintained relatively high expression of NPF genes for a longer time than Jing 411 during seed *** and transcriptome data revealed that KN9204 was more tolerant of nitrogen deficiency than Jing 411,especially at the reproductive *** high NUE of KN9204 is an integrated effect controlled at different *** together,our data provide new insights into the molecular mechanisms of NUE and important gene resources for improving wheat cultivars with a higher NUE trait.

关键词： wheat genome sequencing spatiotemporal gene expression expansion and differentiation of gene family nitrogen use efficiency Kenong 9204

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小鼠卵巢不同发育时期SDF-1/CXCR4轴的动态变化

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生殖医学杂志 2020年第6期29卷 785-791页

作者：何琪马旭金萱夏红飞南昌大学医学院南昌330006 国家卫生健康委科学技术研究所遗传优生中心北京100081 南昌大学第一附属医院辅助生殖中心南昌330006 北京协和医学院研究生院北京100730

目的分析基质细胞衍生因子1(SDF-1)及其受体(CXCR4)在不同发育时期雌性ICR小鼠卵巢组织中的表达及意义。方法选择4周龄、7周龄和10周龄雌性ICR小鼠共30只,根据典型发育时期分为3组:性成熟前期(4周龄)组、性成熟期(7周龄)组及适龄繁殖期... 详细信息

目的分析基质细胞衍生因子1(SDF-1)及其受体(CXCR4)在不同发育时期雌性ICR小鼠卵巢组织中的表达及意义。方法选择4周龄、7周龄和10周龄雌性ICR小鼠共30只,根据典型发育时期分为3组:性成熟前期(4周龄)组、性成熟期(7周龄)组及适龄繁殖期(10周龄)组,每组各10只。每组取5只收集卵巢组织,一侧卵巢提取RNA后利用qPCR检测SDF-1和CXCR4 mRNA表达水平;一侧卵巢固定包埋后切片,采用he染色观察卵巢组织形态并进行各级卵泡计数,采用免疫组化方法观察SDF-1及CXCR4蛋白在小鼠卵巢组织的表达。余下每组5只促排卵后获取卵丘卵母细胞复合物(COCs),分离卵丘颗粒细胞与卵母细胞并提取RNA,利用qPCR分别检测两种细胞中SDF-1和CXCR4的mRNA表达水平。结果3组小鼠中性成熟前期组卵巢SDF-1 mRNA表达量显著高于其他两组(Phe染色显示3个发育阶段小鼠卵巢均可见发育正常的各级卵泡;适龄繁殖期组的卵巢总卵泡数目及初级卵泡数量显著高于其余两组(P<0.01),次级卵泡数显著多于性成熟期组(P<0.01)。免疫组化结果表明:SDF-1及CXCR4蛋白在卵巢各级卵泡的颗粒细胞和卵母细胞中都有表达,而在卵泡膜细胞中几乎不表达。COCs的qPCR结果显示:卵母细胞中,性成熟期组SDF-1 mRNA表达水平显著高于性成熟前期组(P<0.05);卵丘颗粒细胞中,适龄繁殖期组SDF-1 mRNA表达水平显著高于其余两组(P<0.01),性成熟前期组CXCR4 mRNA表达水平显著低于其余两组(P<0.05)。结论SDF-1及其受体CXCR4在不同发育阶段小鼠卵巢卵泡中均有表达且表达水平存在显著差异,提示其可能参与调控小鼠卵泡发育和性腺成熟的过程。

关键词：基质细胞衍生因子1 CXC趋化因子受体卵巢发育

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早期胚胎停育绒毛滋养层细胞病理学变化

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生殖医学杂志 2020年第1期29卷 85-91页

作者：朱旗董艺超张璐马旭夏红飞国家卫生健康委科学技术研究所遗传优生中心北京100081 北京协和医学院研究生院北京100730

目的观察早期胚胎停育(ma)胎盘绒毛滋养层细胞的病理学变化。方法选择2011年12月至2013年12月于河北石家庄第六医院门诊就诊的260例ma患者(孕周0.05));ma组绒毛滋养层细胞的凋亡指数显著高于对照组(P<0.001)。结论胎盘绒毛滋养层细胞中V... 详细信息

目的观察早期胚胎停育(ma)胎盘绒毛滋养层细胞的病理学变化。方法选择2011年12月至2013年12月于河北石家庄第六医院门诊就诊的260例ma患者(孕周ma组),选择该院自愿行人工流产的正常早孕孕妇(孕周ma组及对照组患者的胎盘绒毛组织送至北京龙迈达科技公司制成组织阵列芯片,样本按≤25岁、26~30岁、31~34岁、≥35岁年龄区间分组,每组随机挑选10对(病例及对照)进行分析。采用免疫组化法检测反映胎盘血管发生的血管内皮生长因子(VEGF)、反映滋养层细胞和间质细胞分布情况的细胞角蛋白(CK19)和波形蛋白(Vimentin)在ma组和对照组患者早孕绒毛滋养层细胞中的表达差异;通过Tunel法检测ma组和对照组患者早孕绒毛滋养层细胞的凋亡情况。结果早期ma组绒毛滋养层细胞中VEGF表达量较对照组显著降低(Pma组绒毛滋养层细胞中CK19及Vimentin表达与对照组无统计学差异(P>0.05));ma组绒毛滋养层细胞的凋亡指数显著高于对照组(P<0.001)。结论胎盘绒毛滋养层细胞中VEGF的低表达及细胞凋亡指数的增加与胚胎停育发生密切相关,提示胎盘血管生成减少和滋养层细胞过度凋亡是造成早期胚胎停育的部分原因。

关键词：胚胎停育 VEGF CK19 Vimentin 凋亡

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棕榈酸诱导人绒毛滋养层细胞胰岛素抵抗模型的建立

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生殖医学杂志 2018年第1期27卷 76-81页

作者：管纯一马旭夏红飞国家卫生计生委科学技术研究所北京100081 北京协和医学院北京100730

目的探讨人胎盘绒毛滋养层细胞HTR8-S/Vneo在高脂环境下是否会形成胰岛素抵抗。方法用MTT法测定棕榈酸和胰岛素不影响HTR8-S/Vneo细胞增殖的最适浓度,在该最适浓度下分为四组(对照组、棕榈酸组、胰岛素组、棕榈酸-胰岛素协同处理组)处理... 详细信息

目的探讨人胎盘绒毛滋养层细胞HTR8-S/Vneo在高脂环境下是否会形成胰岛素抵抗。方法用MTT法测定棕榈酸和胰岛素不影响HTR8-S/Vneo细胞增殖的最适浓度,在该最适浓度下分为四组(对照组、棕榈酸组、胰岛素组、棕榈酸-胰岛素协同处理组)处理HTR8-S/Vneo细胞,检测不同处理条件及不同处理时间下,细胞葡萄糖摄取量和细胞内总糖量。结果用MTT法检测不同浓度棕榈酸及胰岛素处理的HTR8-S/Vneo细胞,发现棕榈酸浓度为0.125mmol/L、胰岛素浓度为100nmol/L时,对细胞增殖没有显著影响(P>0.05),故选择该浓度的棕榈酸与胰岛素处理HTR8-S/Vneo细胞。在处理12h、18h、24h、36h分别检测葡萄糖的消耗量和细胞内总糖量,发现与对照组相比,胰岛素处理后葡萄糖消耗量和细胞内总糖量呈上升趋势(P0.05);与棕榈酸单独处理组相比,棕榈酸-胰岛素协同处理组在12h和18h能显著增加细胞内总糖量(P0.05),且与单独胰岛素处理组相比,棕榈酸-胰岛素协同处理组显著降低了葡萄糖消耗量和细胞内总糖量(P<0.05)。结论棕榈酸-胰岛素协同处理24h,引起HTR8-S/Vneo细胞对胰岛素的敏感性降低,HTR8-S/Vneo细胞胰岛素抵抗模型建立;与文献报道模型相比,棕榈酸-胰岛素协同处理大大缩短了细胞出现胰岛素抵抗的时间。

关键词：人绒毛滋养层细胞棕榈酸胰岛素抵抗

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