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MiR-21 and its signaling pathways in the role of gestational diabetes mellitus

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MiR-21 and its signaling pathways in the role of gestational...

The role of miR-125b in placentas during the pathogenesis of gestational diabetes mellitus

中国生理学会生殖科学专业委员会—中国动物学会生殖生物学分会第二次联合学术年会暨“生殖科学专业委员会第二次学术交流会”和“生殖生物学分会第十六次学术年会”

作者： Chun-yi Guan hong-fei xia Reproductive and Genetic Center of National Research Institute for Family Planning Graduate School Peking Union Medical College

MiR-21 played an important role in many diseases,but its role in gestational diabetes mellitus(GDM)and its mechanism is unclear,our research has shown that miR-21 in placenta of patients with gestational diabetes mellitus were differentially expressed in *** chose to use human chorionic trophoblast cells(HTR8-SVneo) study on cell function of *** show that the transfection miR-21 in HTR8-SVneo cells,its function in cell proliferation,infiltration and migration significantly enhanced,and significantly inhibitory effect of cell ***-21 function for further study,we used bioinformatics methods to predict miR-21 target genes found PPARA may as its target *** of PPARA,found that the cell proliferation,infiltration and transfer function were significantly suppressed,PPARA on miR-21 the binding sites of mutation,can inhibit the miR-21 binding of PPARA 3’-UTR,so we determine PPARA is miR-21 target genes,is regulated by the miR-21.

关键词： CSCB,miR-21 GDM PPARA Cell biology behavior

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The role of miR-125b in placentas during the pathogenesis of...

作者： Jian-Hui Li Xue-Qin Wang Xu M hong-fei xia Reproductive and Genetic Center of National Research Institute for Family Planning Graduate School Peking Union Medical College

The placenta has a critical role in the pathogenesis of gestational diabetes mellitus(GDM).MiRNAs in placenta affect the development and metabolism of *** dysregulation of miRNAs will result in the dysfunction of ***,the role of miRNAs in placenta during the pathogenesis of GDM is still *** this study,we find miR-125 b differentially expressed in placentas from patients with GDM and normal controls by chip *** was revealed that miR-125 b expression was lower in GDM placenta compared with *** vitro cell experiment showed that miR-125 b can suppress cell proliferation,promote cell *** establish insulin resistance model with human first-trimester placenta cell HTR-8 and found miR-125 b can inhibit insulin *** online prediction showed that GCNT1 was the target gene of miR-125 *** was also verified that GCNT1 was a direct target of miR-125 b with a specific binding site at the seed sequence,which determines target ***,it is suggested that downregulation of miR-125 b in placenta may be associated with the pathogenesis of GDM via an effect on the regulation of GCNT1 expression.

关键词： GDM miR-125b placenta GCNT1

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新形势下如何加强大学生心理健康教育

教育信息化论坛 2017年第4期1卷 27-28,30页

作者：费红霞太原科技大学

随着我国经济不断发展,大学教育面临新形势的挑战。在新形势下,如何通过心理教育课程实现为学生解决相应心理问题和生活困惑,显得尤为重要。需要广大大学教师通过当前社会实情分析,对学生心理问题成因进行了解,并通过成因“对症下药”,... 详细信息

随着我国经济不断发展,大学教育面临新形势的挑战。在新形势下,如何通过心理教育课程实现为学生解决相应心理问题和生活困惑,显得尤为重要。需要广大大学教师通过当前社会实情分析,对学生心理问题成因进行了解,并通过成因“对症下药”,实现贯彻落实以生为本的教育理念,帮助大学生树立正确的人生观、价值观、世界观,实现为大学生全面发展成功助力。

关键词：新形势大学生心理问题成因全面发展

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从“找不着北”中看转喻

信阳农业高等专科学校学报 2009年第1期19卷 97-98页

作者：费红霞广州大学人文学院广东广州510006

转喻是人类重要的思维方式,生活中的很多语言现象都与转喻有关。转喻必须具备两个条件:一是转喻载体和目标须在同一认知框架内;二是转喻载体须显著度高。"找不着北"的"北"实指东西南北四个方向。

关键词：转喻认知框架显著度

MiR-125b Expression Affects the Proliferation and Apoptosis of Human Glioma Cells by Targeting Bmf

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MiR-125b Expression Affects the Proliferation and Apoptosis ...

苹果全基因组多聚半乳糖醛酸酶基因家族的鉴定及进化分析

“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： hong-fei xia,Tian-Zhu He,Yi Cui,Xu Ma Reproductive and Genetic Center of National Research Institute for Family Planning,Beijing,100081

Background:MicroRNAs(miRNAs) are small noncoding RNAs whose function as modulators of gene expression is crucial for the proper control of cell *** many microRNAs were found to express in central nervous system(CNS),the role of the regulatory networks in which they are involved and their function in the pathological process of nerve cells are only just *** the present study,the possible mechanisms by which one neuronal miRNAs,miR-125b,affected the growth of nervous cells were investigated using in vitro cell line ***:The expression pattern of miR-125b in ATRA-treated human glioma cell lines was detected by Northern blotting and in situ *** effect of miR-125b on the proliferation and apoptosis of human glioma cells was analyzed by MTS assay,TUNEL and Flow cytometry *** addition,the identification of target gene of miR-125b was studied by dualluciferase activity assay and Immunoblot ***:We found differential expression of miR-125b in 1.0 uM all-trans-retinoic acid(ATRA)-treated human glioma cell ***-regulation of miR-125b partially restored cell viability and inhibited cell apoptosis in U343 cells treated by ***-regulation of miR-125b decreased human glioma cells proliferation and enhanced the sensitivity of human glioma cells to ATRA-induced *** addition,we found an inverse relationship between the expression of miR-125b and the cell apoptosis-related protein Bcl-2 modifying factor(Bmf),and miR-125b can interact with 3c-untranslated region(UTR) of ***:These findings indicate that overexpression of miR-l25b promotes human glioma cell proliferation and inhibits ATRA-induced cell apoptosis and low expression of miR-125b sensitizes cells to ATRAinduced *** may play an important role in the process of miR- 125b influencing cell apoptosis.

关键词： miR-125b Human glioma cells Proliferation Apoptosis Bmf

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园艺学报 2016年第10期43卷 1863-1877页

作者：陈鸿飞邵红霞樊胜马娟娟张东韩明玉西北农林科技大学园艺学院陕西杨凌712100

从苹果全基因组中鉴定出85个多聚半乳糖醛酸酶(PG)基因,通过聚类分析将其分成了7个组(Group A^Group G),其分子量在176~1 125 aa之间,等电点在4.68~9.58之间。鉴定出来的Md PG基因除在14号染色体没有分布外,其余都有分布。系统分析了Md... 详细信息

从苹果全基因组中鉴定出85个多聚半乳糖醛酸酶(PG)基因,通过聚类分析将其分成了7个组(Group A^Group G),其分子量在176~1 125 aa之间,等电点在4.68~9.58之间。鉴定出来的Md PG基因除在14号染色体没有分布外,其余都有分布。系统分析了Md PG蛋白的保守基序和Md PG基因结构,发现苹果PG蛋白除包含有广泛存在于各种PG蛋白的4个保守基序以外,还含有其他相对特异的保守基序,Md PG75具有最多的外显子数,达到18个。对85个苹果PG蛋白之间的功能联系网络进行了系统研究,分析预测了相关基因的功能和多个基因间功能的联系,并作了初步验证。

关键词：苹果 PG 家族分析生物信息学

完全腹腔镜胰十二指肠切除术及全胰切除术联合门静脉-肠系膜上静脉切除重建可行性及安全性分析

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中国实用外科杂志 2024年第2期44卷 199-204页

作者：孙蒙清陈少博洪夏飞董良博何小东韩显林中国医学科学院北京协和医院基本外科北京100005

目的探讨完全腹腔镜胰十二指肠切除术(LPD)及腹腔镜全胰切除术(LTP)联合门静脉-肠系膜上静脉(PV-SMV)切除重建的技术要点,及其安全性、可行性和根治性。方法回顾性分析2022年5月至2023年9月在中国医学科学院北京协和医院接受LPD及LTP... 详细信息

目的探讨完全腹腔镜胰十二指肠切除术(LPD)及腹腔镜全胰切除术(LTP)联合门静脉-肠系膜上静脉(PV-SMV)切除重建的技术要点,及其安全性、可行性和根治性。方法回顾性分析2022年5月至2023年9月在中国医学科学院北京协和医院接受LPD及LTP联合PV-SMV切除重建手术的13例病人临床资料。结果 13例病人中,10例病人行LPD,3例病人行LTP。7例(53.8%)病人行PV-SMV侧壁切除缝合,4例(30.8%)病人行PV-SMV节段切除、对端吻合,2例(15.4%)病人行PV-SMV节段切除、人工血管搭桥。手术时间(314.2±70.5)min。术中出血量(442.3±247.4)mL。PV-SMV中位阻断时间22(8~80)min。术后1例病人发生胃排空延迟,未发生B级以上胰瘘、术后出血、腹腔感染、胆瘘、二次手术、急性血栓栓塞事件及30 d内围手术期死亡。术后住院时间(10.7±4.7)d。术后病理学检查结果显示:1例为胰腺伴有破骨巨细胞的未分化癌,12例为胰腺导管腺癌。标本肿瘤大小(3.9±1.6)cm。术中淋巴结清扫(25.7±12.7)枚。淋巴结转移9例(69.2%)。术后病理学检查证实侵犯门静脉者5例(38.5%)。所有病人均为R0切除。术后门静脉通畅率100.0%。结论对于经验丰富的微创胰腺手术团队,在合理选择适应证的基础上,LPD或LTP联合PV-SMV切除重建用于部分胰头癌侵犯PV-SMV的病人是安全可行的,可提高肿瘤的R0切除率,使病人获益。

关键词：腹腔镜胰十二指肠切除术腹腔镜全胰切除术胰腺癌门静脉-肠系膜上静脉切除重建

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模板中热解聚丙烯腈制备碳纳米管阵列

四川大学学报（工程科学版） 2004年第6期36卷 51-54页

作者：熊善新王琪陈英红费国霞高分子材料工程国家重点实验室四川大学高分子研究所四川成都610065

采用模板合成技术,以具有规整孔洞结构的阳极氧化铝膜(AAO)为模板,制备有序排列的碳纳米管阵列。制备过程是在AAO模板中通过化学聚合法,首先制备聚丙烯腈(PAN)纳米管阵列,然后将PAN纳米管于750℃,氮气气氛中热解,制备碳纳米管阵列。用SE... 详细信息

采用模板合成技术,以具有规整孔洞结构的阳极氧化铝膜(AAO)为模板,制备有序排列的碳纳米管阵列。制备过程是在AAO模板中通过化学聚合法,首先制备聚丙烯腈(PAN)纳米管阵列,然后将PAN纳米管于750℃,氮气气氛中热解,制备碳纳米管阵列。用SEM、TEM和XRD表征聚丙烯腈纳米管和碳纳米管的结构和形貌,并探讨竹节状碳纳米管的形成机理。结果表明,两种纳米管的管径约为200nm,与模板孔道的孔径相当,纳米管能排列成有序的阵列结构;XRD分析证实碳纳米管具有较高的结晶度;TEM照片显示碳纳米管具有独特的竹节状结构,可能是由于聚丙烯腈纳米管的热解产物在模板内不均匀沉积和碳化造成的。

关键词：模板合成聚丙烯腈纳米管碳纳米管纳米阵列热解

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu Yan Yu xiao-Li Li xiao-Ting Sun Li-Ting Wang xia Wu Ling-Jie Zhu xiao-Jia Yang Qian-Ru He fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors