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MOSPD2 is a receptor mediating the LEAP-2 effect on monocytes/macrophages in a teleost,Boleophthalmus pectinirostris

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Zoological Research 2020年第6期41卷 644-655页

作者： chang-hong li Jie Chen li Nie Jiong Chen State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Ningbo UniversityNingboZhejiang 315211China Laboratory of Biochemistry and Molecular Biology School of Marine SciencesMeishan CampusNingbo UniversityNingboZhejiang 315832China Key Laboratory of Applied Marine Biotechnology of Ministry of Education Meishan CampusNingbo UniversityNingboZhejiang 315832China College of Ecology Lishui UniversityLishuiZhejiang 323000China

liver-expressed antimicrobial peptide 2(LEAP-2)is a cationic peptide that plays an important role in a host’s innate immune *** previously demonstrated that mudskipper(Boleophthalmus pectinirostris)LEAP-2(BpLEAP-2)induces chemotaxis and activation of monocytes/macrophages(MO/MΦ).However,the molecular mechanism by which BpLEAP-2 regulates MO/MΦ remains *** this study,we used yeast twohybrid cDNA library screening to identify mudskipper protein(s)that interacted with BpLEAP-2,and characterized a sequence encoding motile sperm domain-containing protein 2(BpMOSPD2).The interaction between BpLEAP-2 and BpMOSPD2 was subsequently confirmed by co-immunoprecipitation(Co-IP).Sequence analyses revealed that the predicted BpMOSPD2 contained an N-terminal extracellular portion composed of a CRAL-TRIO domain and a motile sperm domain,a C-terminal transmembrane domain,and a short cytoplasmic *** tree analysis indicated that BpMOSPD2 grouped tightly with fish MOSPD2 homologs and was most closely related to that of the Nile tilapia(Oreochromis niloticus).The recombinant BpMOSPD2 was produced by prokaryotic expression and the corresponding antibody was prepared for protein concentration *** interference was used to knockdown BpMOSPD2 expression in the mudskipper MO/MΦ,and the knockdown efficiency was confirmed by quantitative real-time polymerase chain reaction(qRT-PCR)and western *** of BpMOSPD2 significantly inhibited BpLEAP-2-induced chemotaxis of mudskipper MO/MΦand BpLEAP-2-induced bacterial killing ***,knockdown of BpMOSPD2 inhibited the effect of BpLEAP-2 on mRNA expression levels of BpIL-10,BpTNFα,BpIL-1β,and BpTGFβ in MO/MΦ.In general,BpMOSPD2 directly interacted with BpLEAP-2,and mediated the effects of BpLEAP-2 on chemotaxis and activation of mudskipper MO/MΦ.This is the first identification of MOSPD2 as a receptor for LEAP-2.

关键词： LEAP-2 Monocyte/macrophage MOSPD2 RNA interference Yeast two-hybrid

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Characterization and expression of sweetfish （Plecoglossus altivelis） cathepsin D

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Zoological Research 2014年第4期35卷 294-299页

作者： Yu JIAO chang-hong li Xin-Jiang LU Jiong CHEN Key Laboratory of Applied Marine Biotechnology Ministry of Education Ningbo University

Cathepsin D （CTSD） is a lysosomal acidic endoproteinase that plays an important role in immune response, In this study, we obtained sweetfish （Plecoglossus altivelis） CTSD （PaCTSD） via de-novo transcriptome sequencing of sweetfish macrophages. The full length cDNA sequence of PaCTSD was 1955 bp encoding a propeptide of 397 amino acids. The deduced protein had a calculated molecular weight of 43.17x 103. Multiple alignment with other known CTSD amino acid sequences revealed amino acid conservation through the teleosts. Phylogenetic tree analysis showed that PaCTSD grouped tightly with other fish CTSD, and was close to that of Atlantic salmon and rainbow trout. Subsequently, PaCTSD was prokaryotically expressed and refolded by the urea gradient method on a nickel-nitrilotriacetic acid column. Enzyme activity analysis showed that PaCTSD exhibited pH-dependent proteolytic activity. Quantitative real-time PCR showed that PaCTSD mRNA was expressed in all detected tissues in healthy sweetfish. The highest expression was observed in the spleen and white blood cells, followed by liver, head-kidney, kidney, intestine, gill, and muscle. After listonella anguillarum infection, PaCTSD transcripts were up-regulated significantly in liver, spleen, white blood cells, and head-kidney of sweetfish. In summary, PaCTSD has proteolytic activity and is closely involved in the immune response of sweetfish.

关键词： Cathepsin D Plecolossus altivelis Bacterial infection Prokaryotic expression qRT-PCR

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Grasshopper （Orthoptera： Acrididae） biodiversity and grassland ecosystems

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Insect Science 2006年第3期13卷 221-227页

作者： Zhong-WEI GUO hong-chang li YA-liNG GAN Key Laboratory of Animal Ecology and Conservation Biology State Key Laboratory of Integrated Mangement of Pest Insectsand Rodents Institute of Zoology Chinese Academy of Sciences Beijing China

Interesting results may arise by combining studies on the structure and function of ecosystems with that of biodiversity for certain species. Grasshopper biodiversity is the result of the evolution of grassland ecosystems; however, it also impacts on the structure and the function of those ecosystems. We consider there to be a close relationship between the health of grassland ecosystems and grasshopper biodiversity. The main problems involved in this relationship are likely to include：（i） grasshopper biodiversity and its spatial pattern; （ii） the effect of grasshopper biodiversity on the ecological processes of grassland ecosystems; （iii） the biodiversity threshold of grasshopper population explosions; （iv） the relationship between grasshopper biodiversity and the natural and human factors that affect grassland ecosystems; and （v） grasshopper biodiversity and the health of grassland ecosystems. The solutions to these problems may provide sound bases for controlling disasters caused by grasshoppers and managing grassland ecosystems in the west of China. In this paper, we introduced two concepts for grasshopper biodiversity, that is, ＂spatial pattern＂ and ＂biodiversity threshold＂. It is helpful to understand the action of the spatial pattern of grasshopper biodiversity on the ecological processes of grassland ecosystems and the effect of this spatial pattern on the health of those ecosystems, owing to the fact that, in the west of China, grasslands are vast and grasshoppers are widely distributed. Moreover, we inferred that the change in the level of component richness at each type of grasshopper biodiversity can make an impact on grassland ecosystems, and therefore, there is likely to be a threshold to grasshopper biodiversity for the stability and the sustainability of those ecosystems.

关键词： biodiversity grasshopper grassland ecosystem spatial pattern threshold

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Columnar to Equiaxed Transition During Solidification of Small Ingot by Using Electric Current Pulse

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Journal of Iron and Steel Research International 2009年第6期16卷 7-12页

作者： li Jie MA Jian-hong SONG chang-jiang li Zhi-jun GAO Yu-lai ZHAI Qi-jie Shanghai Key Laboratory of Modern Metallurgy and Materials Processing Shanghai University Shanghai 200072 China

A new approach to applying the electric current pulse （ECP） with parallel electrodes to the promotion of the transition from columnar crystal to equiaxed crystal and the improvement of macrosegregation was introduced. The ECP was applied to different stages of the solidification. The results showed that the application of the ECP in both the initial stage （the thickness of solidified shell reached 2 mm approximately） and the late stage （the thickness of solidified shell reached 14 mm approximately） of solidification can promote the columnar to equiaxed transition （CET）. The analysis showed that during solidification, a large number of nuclei around the upper surface fell off due to ECP, which subsequently showered on the melt and impinged the growth front of the columnar crystal. Therefore, the CEToccurred. In addition, this method was also employed to influence the solidification process of bearing steel, and the results showed that the structure was changed from columnar crystal to equiaxed crystal, indicating that ECP can enhance the homogeneity of structure and composition of bearing steel.

关键词： columnar to equiaxed transition solidification electric current pulse macrosegregation

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Molecular characterization of an IL-1β gene from the large yellow croaker（Larimichthys crocea） and its effect on fish defense against Vibrio alginolyticus infection

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Zoological Research 2015年第3期36卷 133-141页

作者： Jun WU Yu-hong SHI Xue-Heng ZHANG chang-hong li Ming-Yun li Jiong CHEN Laboratory of Biochemistry and Molecular Biology School of Manne Sciences Ningbo University Ningbo 315211 China Donghai Sea Collaborative Innovation Center for Industrial Upgrading Mariculture Ningbo University Ningbo 315211 China

Interleukin 1β（IL-1β）, the first interleukin to be characterized, plays a key role in regulating the immune response. In this study, we determined the c DNA and genomic DNA sequences of the IL-1β gene from the large yellow croaker, Larimichthys crocea. Phylogenetic analysis indicated that the IL-1β（Lc IL-1β） gene was most closely related to that of European seabass（Dicentrarchus labrax）, sharing 67.8% amino acid identity. In healthy large yellow croaker, Lc IL-1β transcription was detected in all tested tissues, with the highest level found in the head kidney. Upon Vibrio alginolyticus infection, Lc IL-1β transcription in all tested tissues was significantly upregulated. Intraperitoneal injection of recombinant Lc IL-1β（r Lc IL-1β） improved the survival rate and reduced the tissue bacterial load after V. alginolyticus infection. In addition, r Lc IL-1β induced monocytes/macrophages（MO/MΦ） chemotaxis and increased phagocytosis and bactericidal activity in vitro. These results suggest that Lc IL-1β plays an important role in the large yellow croaker immune response against V. alginolyticus.

关键词： Interleukin 1β Large yellow croaker Survival rate Vibrio alginolyticus Monocytes/macrophages

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Outcomes of 13 ICSI-PGD cycles with ejaculated spermatozoa in patients with Klinefelter syndrome

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Asian Journal of Andrology 2016年第3期18卷 498-499页

作者： Tian-Xiang Ni Jun-Hao Yan Bo Wang Yue-Ting Zhu hong-chang li hong-Qiang Xie Wen-Jie Jiang Zi-Jiang Chen Center for Reproductive Medicine Shandong Provincial Hospital Affiliatedto Shandong University Shandong Provincial Key Laboratory of Reproductive Medicine Jinan 250021 China National Research Center for Assisted Reproductive Technology and Reproductive Genetics Jinan 250021 China The Key Laboratory for Reproductive Endocrinology of Ministry of Education Jinan 250021 China Department of Anesthesiology Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021 China.

Dear Editor, Klinefelter syndrome （KS） is the most frequent genetic cause of infertility in men. Paternity can be achieved through intracytoplasmic sperm injection （ICSI） with spermatozoa recovered from ejaculated... 详细信息

关键词： @@

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MICROCALORIMETRY ON BACILLUS ***-61

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Chinese Chemical Letters 1991年第9期2卷 709-710页

作者： Wei hong XIE chang li XIE Shong Sheng QU Department of Chemistry,Wuhan University,Wuhan 430072

The fundamental growth thermograms of Bacillus ***-61 have been de- termined by microcalorimetric *** these growth curves we got some thermo- kinetics data of its growth and its thermodynamic properties.

关键词： TT SP MICROCALORIMETRY ON BACILLUS SP.NTT-61

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Quantum public-key crypto via EPR pairs

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 58-63页

作者： Xiang-Qun Fu hong-Wei li Jian-hong Shi Tan li Wan-Su Bao Henan Key Laboratory of Quantum Information and Cryptography Information Engineering University Synergetic Innovation Center of Quantum Information and Quantum Physics University of Science and Technology of China

With the development of quantum computing technology, quantum public-key cryptography is gradually becoming an alternative to the existing classical public-key cryptography. This paper designs a quantum trapdoor one-way function via EPR pairs and quantum measurements. Based on this, a new quantum public-key cryptosystem is presented, which offers forward security,and can resist the chosen-plaintext attack and chosen-ciphertext attack. Compared with the existing quantum public-key cryptos,eavesdropping can be automatically detected in this new quantum public-key cryptosystem under a necessary condition, which is also detailed in the paper.

关键词： quantum public-key crypto trapdoor one-way function EPR pairs

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Sequence-activated chemiluminescence probes: programmable illumination for high-contrast in vivo sensing and imaging

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Science China Chemistry 2025年第1期68卷 26-34页

作者： Yutao Zhang Juan li Wei-hong Zhu Zhiqian Guo Shanghai Key Laboratory of Functional Materials Chemistry Joint International Research Laboratory of Precision Chemistry and Molecular Engineering Institute of Fine Chemicals School of Chemistry and Molecular Engineering East China University of Science and Technology State Key Laboratory of Bioreactor Engineering Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell MetabolismEast China University of Science & Technology

Sequence-activated chemiluminescence(CL) technique offers a unique approach to detecting specific biomolecules or cellular events with high sensitivity and spatial resolution. The fundamental principle behind sequence-activated CL probes involves tailoring highly-controllable CL signals in response to specific sequences or events of interest, thus enabling high-contrast signals under complex physiological conditions. This sequential activation could be triggered by enzymes, reactive oxygen species(ROS), biothiols or changes in the microenvironment. These unique sequence-activated CL probes bestow several advantages, including minimal background, deep-tissue penetration, and the ability of non-invasive or minimally invasive detection. The sequence-activated CL techniques represent a significant advancement in molecular imaging and diagnostics. In this mini-review, we focus on the latest developments in sequential CL probes, addressing the technical challenges and discussing the future trajectory by harnessing CL signaling and molecular recognition.

关键词： chemiluminescnece sequence-activation in-vivo bioimaging cyclic peroxide

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Tailoring light–Matter Interactions in Overcoupled Resonator for Biomolecule Recognition and Detection

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Nano-Micro Letters 2025年第1期17卷 262-280页

作者： Dongxiao li hong Zhou Zhihao Ren Cheng Xu Chengkuo Lee Department of Electrical and Computer Engineering National University of SingaporeSingapore 117583Singapore Center for Intelligent Sensors and MEMS(CISM) National University of SingaporeSingapore 117608Singapore

Plasmonic nanoantennas provide unique opportunities for precise control of light–matter coupling in surface-enhanced infrared absorption(SEIRA)spectroscopy,but most of the resonant systems realized so far suffer from the obstacles of low sensitivity,narrow bandwidth,and asymmetric Fano resonance ***,we demonstrated an overcoupled resonator with a high plasmon-molecule coupling coefficient(μ)(OC-Hμresonator)by precisely controlling the radiation loss channel,the resonator-oscillator coupling channel,and the frequency detuning *** observed a strong dependence of the sensing performance on the coupling state,and demonstrated that OC-Hμresonator has excellent sensing properties of ultra-sensitive(7.25%nm^(−1)),ultra-broadband(3–10μm),and immune asymmetric Fano *** characteristics represent a breakthrough in SEIRA technology and lay the foundation for specific recognition of biomolecules,trace detection,and protein secondary structure analysis using a single array(array size is 100×100μm^(2)).In addition,with the assistance of machine learning,mixture classification,concentration prediction and spectral reconstruction were achieved with the highest accuracy of 100%.Finally,we demonstrated the potential of OC-Hμresonator for SARS-CoV-2 *** findings will promote the wider application of SEIRA technology,while providing new ideas for other enhanced spectroscopy technologies,quantum photonics and studying light–matter interactions.

关键词： Plasmonic nanoantennas light-matter interaction Surface-enhanced infrared absorption Overcoupled Biosensing

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