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Precore/basal core promoter mutants quantification throughout phases of hepatitis B virus infection by Simpleprobe

World Journal of Gastroenterology 2015年第21期21卷 6639-6648页

作者： Wen-Hui Tu Ying Lv Yong-Mei Zhang Wei Hou Jin-Yu Wang Yi-Jun Zhang hong-yan liu Hao-Xiang Zhu yan-Li Qin Ri-Cheng Mao Ji-Ming Zhang Department of Infectious Disease Huashan HospitalFudan University Department of Infectious Disease Taizhou Municipal Hospital Department of Infectious Disease Public Health Clinical Center of ShanghaiFudan University

AIM:To investigate precore/basal core promoter(PC/BCP) mutants throughout hepatitis B virus(HBV) infection and to determine their relationship to hepatitis B early antigen(HBeA g) ***:We enrolled 191 patients in various stages of HBV infection at the Huashan Hospital and the Taizhou Municipal Hospital from 2010 to *** of the patients received antiviral *** DNA from serum,was quantified by real-time *** HBV genotype was determined by direct sequencing of the S *** used the Simpleprobe ultrasensitivequantitative method to detect PC/BCP mutants in each *** compared the strain number,percentage,and the changes in PC/BCP mutants in different phases,and analyzed the relationship between PC/BCP mutants and HBe Ag by multiple linear regression and logistic ***:Patients with HBV infection(n = 191) were assigned to groups by phase:Immune tolerance(IT) = 55,Immune clearance(IC) = 67,Low-replicative(LR) = 49,and HBeA g-negative hepatitis(ENH) = *** the patients(male,112; female,79) enrolled,122 were HBe Ag-positive and 69 were HBe *** median age was 33 years(range:18-78 years).PC and BCP mutation detection rates were 84.82%(162/191) and 96.86%(185/191),*** five HBe Ag-negative cases,we detected double mutation G1896A/G1899 *** logarithm value of PC mutant quantities(log10 PC) significantly differed in IT,IC,and LR phases,as well as in the ENH phase(F = 49.350,P < 0.001).The logarithm value of BCP mutant quantities(log10 BCP) also differed during the four phases(F = 25.530,P < 0.001).Log10 PC and log10 BCP values were high in the IT and IC phases,decreased in the LR phase,and increased in the ENH phase,although the absolute value at this point remained lower than that in the IT and IC *** mutant quantity per total viral load(PC%) and BCP mutant quantity per total viral load(BCP%) differed between phases(F = 20.040,P < 0.001; F = 10.830,P < 0.001),with PC% and BCP% gradually increasing in suc

关键词： precore mutant basal core promotermutant Hepatitis B virus Quantification Hepatitis Bearly antigen titers

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Atypical Whipple’s disease with special endoscopic manifestations:A case report

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World Journal of Clinical Cases 2022年第35期10卷 13044-13051页

作者： Shuo Chen Yuan-Chen Zhou Shuang Si hong-yan liu Qing-Rui Zhang Teng-Fei Yin Chu-Xi Xie Shu-KunYao Shi-Yu Du Department of Gastroenterology China-Japan Friendship HospitalBeijing 100029China Peking University China-Japan Friendship School of Clinical Medicine Peking UniversityBeijing 100029China Department of General Surgery China-Japan Friendship HospitalBeijing 100029China Department of Pathology China-Japan Friendship HospitalBeijing 100029China Graduate School Beijing University of Chinese MedicineBeijing 100029China

BACKGROUND Whipple’s disease is a rare systemic infection caused by Tropheryma *** patients present with nonspecific symptoms,and routine laboratory and imaging examination results also lack *** diagnosis often relies on invasive manipulation,pathological examination,and molecular *** difficulties in diagnosing Whipple’s disease often result in misdiagnosis and inappropriate *** SUMMARY This paper reports on the case of a 58-year-old male patient who complained of fatigue and decreased exercise *** results of routine blood tests indicated hypochromic microcytic *** of gastroscopy and capsule endoscopy showed multiple polypoid bulges distributed in the duodenal and proximal jejunum.A diagnosis of small intestinal adenomatosis was initially considered;hence,the Whipple procedure,a pylorus-preserving pancreaticoduodenectomy,was *** manifestations showed many periodic acid-Schiff-positive macrophages aggregated in the intestinal mucosa of the duodenum,upper jejunum,and surrounding lymph *** on comprehensive analysis of symptoms,laboratory findings,and pathological manifestations,the patient was finally diagnosed with Whipple’s *** receiving 1 mo of antibiotic treatment,the fatigue and anemia were significantly *** This case presented with atypical gastrointestinal manifestations and small intestinal polypoid bulges,which provided new insight on the diagnosis of Whipple’s disease.

关键词： Whipple’s disease Whipple operation Endoscopy Tropheryma whipplei PAS staining Intestinal polyposis Case report

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Optimization,validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC–MS/MS

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Journal of Pharmaceutical Analysis 2015年第6期5卷 383-388页

作者： Jing Wang Ji-Ye Sun Chun-Jie Sha Yu-Feng Shao yan-hong liu You-Xin Li Zhen-Wen Duan Wan-Hui liu School of Pharmacy Yantai University State Key Laboratory of Long-acting and Targeting Drug Delivery System Shandong Luye Pharmaceutical Co. Ltd. Peking University WBL Biotech Co. Ltd.

A stable HMG-CoA reductase （HMGR） reaction in vitro was developed by a sensitive, selective and precise liquid chromatography-tandem mass spectrometry （LC-MS/MS） method. The optimized enzyme reaction condition contained 1.5 lag of HMGR, 20 nM of NADPH with 50 rain of reaction time. The method was validated by several intraand inter-day assays. The production transitions of m/z 147.0/59.1 and m/z 154.0/59.1 were used to detect and quantify mevalonolactone （MVAL） and MVAL-DT, respectively. The accuracy and precision of the method were evaluated over the concentration range of 0.005- 1.000 lag/mL for MVAL and 0.010-0.500 lag/mL for lovastatin acid in three validation batch runs. The lower limit of quantitation was found to be 0.005 lag/mL for MVAL and 0.010 lag/mL for lovastatin acid. Intra-day and inter-day precision ranged from 0.95% to 2.39% and 2.26% to 3.38% for MVAL, 1.46% to 3.89% and 0.57% to 5.10% for lovastatin acid, respectively. The results showed that the active ingredients in Xuezhikang capsules were 12.2 and 14.5 mg/g, respectively. This assay method could be successfully anDlied to the oualitv control study of Xuezhikanu caosule for the first time.

关键词： XuezhikangLC-MS/MSHMG-CoA reductaseMevalonolactoneQuality control

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An Efficient Synthesis and Screening of Osteostatin analogues by Using Pooling Strategy

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Chinese Chemical Letters 1999年第10期10卷 821-824页

作者： De Xin WANG Rui HAN hong yan liu Lei HUANG Jun FU(Institute of Materia Medica. Chinese Academy of Medical Sciencesand Peking Union Medical College, Beijing 100050) Institute of Materia Medica Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100050 China

An efficient synthesis and screening of twenty one osteostatin(OSN) analogues were achieved by using pooling strategy. Three analogues were found to retain almost the same activity of inhibiting bone resorption in rat as that of OSN. Therefore, it is possible to develop partially modified OSN surrogate with higher enzymic stability.

关键词： structure modification pooling strategy OSN analogues osteoporosis

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Association of cumulative resting heart rate exposure with rapid renal function decline:a prospective cohort study with 27,564 older adults

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Journal of Geriatric Cardiology 2023年第9期20卷 673-683页

作者： Xi JIANG Xian SHAO Xing LI Pu-Fei BAI hong-yan liu Jia-Mian CHEN Wei-Xi WU Zhuang CUI Fang HOU Chun-Lan LU Sai-Jun ZHOU Pei YU NHC Key Laboratory of Hormones and Development Chu Hsien-I Memorial Hospital and Tianjin Institute of EndocrinologyTianjin Medical UniversityTianjinChina Tianjin Key Laboratory of Metabolic Diseases Tianjin Medical UniversityTianjinChina Ordos Center Hospital OrdosInner MongoliaSudu StreetKangbashi DistrictOrdos CityChina Department of Epidemiology and Health Statistics Tianjin Medical UniversityHeping DistrictTianjinChina Community Health Service Center Jiefang RoadTanggu StreetBinhai New DistrictTianjinChina

OBJECTIVE To evaluate the prospective association between cumulative resting heart rate(cumRHR)and rapid renal function decline(RRFD)in a cohort of individuals aged 60 and *** In the Tianjin Chronic Kidney Disease Cohort Study,the individuals who underwent three consecutive physical examinations between 2014 and 2017,with estimated glomerular filtration rate(eGFR)greater than 60 mL/min per 1.73 m2 and aged 60 years or older were enrolled.A total of 27,564 patients were prospectively followed up from January 1,2017 to December 31,*** 3-year cumRHR was *** primary outcome was RRFD,defined as an annualized decline in eGFR of 5 mL/min per 1.73 m2 or *** and restricted spline regression models and subgroup analysis were used to investigate the association of cumRHR with RRFD after adjusting for all *** During a median follow-up of 3.2 years,a total of 4,347(15.77%)subjects developed *** fully-adjusted models,compared with the lowest quartile of cumRHR,the odds ratio(OR)for the highest was 1.44(1.28–1.61),P<***,each 1-standard deviation(27.97 beats/min per year)increment in cumRHR was associated with a 17%(P<0.001)increased risk of RRFD,with a linear positive correlation(P for non-linear=0.803).Participants with a 3-year cumRHR≥207(beats/min)*year(equivalent to≥69 beats/min per year in 3 years)were found to be at a higher risk of *** The cumRHR is significantly associated with a higher risk of RRFD among older *** results might provide an effective goal for managing and delaying the decline of renal function in the older adults.

关键词： prospective Kidney function

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Microstructures and Hardness of 8CrWMoV Steel with Multiple Types of Ultra Fine Carbides

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材料热处理学报 2004年第5期25卷 279-283页

作者： DAIYu-mei ZHANGZhan-ping MAYong-qing QIYu-hong liuyan-xia YUTao Instituteofmaterialsandtechnology DalianMaritimeUniversityDalian116026

The structure and hardness of 8CrWMoV steel with multiple types of ultra fine carbides are studied after annealing, quenching and tempering in this paper. The results show that multiple types of carbides M3C, M7C3, M23C6, M6C and MC were observed in the annealed steel. Nucleation and coalescence of new carbides, partial dissolution of original carbides in γ phase region during annealing at 800～840℃, result in ultra-fine carbides. Average size of the carbides is0.33～0.34μm in the steel annealed at 800～840℃. Because M3C and M23C6 dissolve easily in austenite, the high hardness HRC63～65 can be obtained by quenching at 840～860℃. Un-dissolved carbides M6C and MC (VC) can effectively prevent the coarsening of austenitic grain, and conduce to obtain very fine martensite. The retained austenite can be easy to decompose during tempering at low and middle temperature due to the precipitation of multiple types of carbides and the good tempering-resistance of the steel is obtained. The microstructure and property of the steel after heat treatment can be accurately explained by calculating based on phase equilibrium *** Words: 8CrWMoV steel, ultra-fine carbide, heat treatment, microstructure,

关键词：显微结构 8CrWMoV钢超细硬质合金硬度热处理

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Fecal microbiota transplantation induces remission of infantile allergic colitis through gut microbiota re-establishment

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World Journal of Gastroenterology 2017年第48期23卷 8570-8581页

作者： Sheng-Xuan liu Yin-Hu Li Wen-Kui Dai Xue-Song Li Chuang-Zhao Qiu Meng-Ling Ruan Biao Zou Chen Dong yan-hong liu Jia-Yi He Zhi-Hua Huang Sai-Nan Shu Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina Department of Microbial Research We Health Gene InstituteShenzhen 518000Guangdong ProvinceChina Department of Computer Science College of Science and EngineeringCity University of Hong KongHong KongChina

AIM To investigate the impact of fecal microbiota transplantation(FMT) treatment on allergic colitis(AC) and gut microbiota(GM).METHODS We selected a total of 19 AC infants,who suffered from severe diarrhea/hematochezia,did not relieve completely after routine therapy or cannot adhere to the therapy,and were free from organ congenital malformations and other contraindications for FMT. Qualified donor-derived stools were collected and injected to the AC infants via a rectal tube. Clinical outcomes and follow-up observations were noted. Stools were collected from ten AC infants before and after FMT,and GM composition was assessed for infants and donors using 16 S r DNA sequencing *** After FMT treatment,AC symptoms in 17 infants were relieved within 2 d,and no relapse was observed in the next 15 mo. Clinical improvement was also detected in the other two AC infants who were lost to followup. During follow-up,one AC infant suffered from mild eczema and recovered shortly after hormone therapy. Based on the 16 S r DNA analysis in ten AC infants,most of them(n = 6) had greater GM diversity after FMT. As a result,Proteobacteria decreased(n = 6) and Firmicutes increased(n = 10) in post-FMT AC infants. Moreover,Firmicutes accounted for the greatest proportion of GM in the patients. At the genus level,Bacteroides(n = 6),Escherichia(n = 8),and Lactobacillus(n = 4) were enriched in some AC infants after FMT treatment,but the relative abundances of Clostridium(n = 5),Veillonella(n = 7),Streptococcus(n = 6),and Klebsiella(n = 8) decreased *** FMT is a safe and effective method for treating pediatric patients with AC and restoring GM balance.

关键词： Pediatric Infantile allergic colitis Fecal microbiota transplantation Gut microbiota Immune reaction

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A primary study on mutation of HPRT exposure to γ ray andbenzo-pyrene

A primary study on mutation of HPRT exposure to γ ray andbe...

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中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者：刘建功 hong-yan liu Xu-hong DANG China Institute for Radiation Protection

Objective To investigate the changes of mutation of HPRT exposure to γ ray and benzopyrene,in order to try to find new differential diagnosis methods for cell injury induced byγ ray and benzo-pyrene. Methods Human peripheral blood samples from three personswere exposed to 60 Co γ-rays at 10 different doses(0-5Gy) and benzo-pyrene at 6 differentconcentrations(0-10μg/ml), its DNA was isolated from white cell. The changes of mutationof HPRT exposure to γ ray and benzo-pyrene were detected by real-time quantitative *** The relationship between mutation frequency of HPRT and irradiation dose represen tscertainline correlation from 0Gy, alinearregressionequa-tion(y=0.4298+0.0823 x,P0.05, R2=0.450). There is an obvious influenceon mutation frequency of exon 5 of HPRT induced byγray,while there is no obvious influence on mutation frequency of exon 5 of HPRT induced by benzo-pyrene,which may be a new differential diagnosis index in the future.

关键词： γ ray benzo-pyrene mutation frequency HPRT

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Versatile and controlled quantum teleportation network

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Chinese Physics B 2024年第3期33卷 415-423页

作者：周瑶瑶梅鹏娴刘艳红吴量李雁翔闫智辉贾晓军 Department of Physics Taiyuan Normal UniversityJinzhong 030619China College of Information Engineering Shanxi Vocational University of Engineering Science and TechnologyJinzhong 030619China Shanxi North Machinery Manufacturing Co. LtdTaiyuan 030000China State Key Laboratory of Quantum Optics and Quantum Optics Devices Institute of Opto-ElectronicsShanxi UniversityTaiyuan 030006China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China

A quantum teleportation network involving multiple users is essential for future quantum *** far,controlled quantum teleportation has been demonstrated in a three-user ***,versatile and controlled quantum teleportation network involving more users is in demand,which satisfies different combinations of users for practical *** we propose a highly versatile and controlled teleportation network that can switch among various combinations of different *** use a single continuous-variable six-partite Greenberger-Horne-Zeilinger(GHZ)state to realize such a task by choosing the different measurement and feedback *** controlled teleportation network,which includes one sub-network,two sub-networks and three sub-networks,can be realized for different application of user ***,the coherent feedback control(CFC)can manipulate and improve the teleportation *** approach is flexible and scalable,and would provide a versatile platform for demonstrations of complex quantum communication and quantum computing protocols.

关键词： quantum teleportation coherent feedback control versatile controlled transformation

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Manufacture the conducting polymer with light

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Science China Chemistry 2025年第1期68卷 8-9页

作者： yang Li Tianyu Li yan Ji Baoguang liu College of Electronic and Optical Engineering & College of Flexible Electronics (Future Technology) Nanjing University of Posts & Telecommunications

Poly(3,4-ethylenedioxythiophene) (PEDOT), heralded as the “king” of conducting polymers, is esteemed in bioelectronics for its mixed electronic and ionic conductivity, mechanical properties compatible with biological tissues, tunable healing ability and stability in diverse environments [1]. Based on additive manufacturing of three-dimensional structures,PEDOT realizes the implementation in the efficient production of flexible bioelectronics, the construction of intricate electrode-neural implantations, and the development of smart brain-computer-interfaces [2].

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