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Protective Effect of Angiotensin(1-7) on Silicotic Fibrosis in Rats

Biomedical and Environmental Sciences 2019年第6期32卷 419-426页

作者： ZHANG Bo Nan XU hong GAO Xue Min ZHANG Gui zhen ZHANG Xin YANG Fang School of Public Health North China University of Science and Technology Tangshan 063210 Hebei China Hebei Key Laboratory for Organ Fibrosis Medical Research Center North China University of Science and Technology Tangshan 063210 Hebei China Hebei Key Laboratory for Chronic Diseases Tangshan Key Laboratory for Clinical and Basic Research on Chronic Diseases School of Basic Medical Sciences North China University of Science and Technology Tangshan 063210 Hebei China Basic Medical College Hebei Medical University Shijiazhuang 050017 Hebei China

Objective Silicosis, caused by inhalation of silica dust, is the most serious occupational disease in China and the aim of present study was to explore the protective effect of Ang (1-7) on silicotic fibrosis and myofibroblast differentiation induced by Ang Ⅱ. Methods HOPE-MED 8050 exposure control apparatus was used to establish the rat silicosis model. Pathological changes and collagen deposition of the lung tissue were examined by H.E. and VG staining, respectively. The localizations of ACE2 and a-smooth muscle actin (a-SMA) in the lung were detected by immunohistochemistry. Expression levels of collagen type Ⅰ, a-SMA, ACE2, and Mas in the lung tissue and fibroblasts were examined by western blot. Levels of ACE2, Ang (1-7), and Ang Ⅱ in serum were determined by ELISA. Co-localization of ACE2 and a-SMA in fibroblasts was detected by immunofluorescenee. Results Ang (1-7) induced pathological changes and enhanced collagen deposition in vivo. Ang (1-7) decreased the expressions of collagen type Ⅰ and a-SMA and increased the expressions of ACE2 and Mas in the silicotic rat lung tissue and fibroblasts stimulated by Ang Ⅱ. Ang (1-7) in creased the levels of ACE2 and Ang (1-7) and decreased the level of Ang Ⅱ in silicotic rat serum. A779 enhanced the protective effect of Ang (1-7) in fibroblasts stimulated by Ang Ⅱ. Conclusion Ang (1-7) exerted protective effect on silicotic fibrosis and myofibroblast differentiation induced by Ang Ⅱ by regulating ACE2-Ang (l-7)-Mas axis.

关键词： Silicosis Ang Ⅱ Myofibroblasts Ang (1-7) A779

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Polyglutamic Acid Based Polyanionic Shielding System for Polycationic Gene Carriers

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Chinese Journal of Polymer Science 2016年第3期34卷 316-323页

作者： Jia-liang Xia 田华雨 Jie Chen Zhao-pei Guo lin lin hong-yan Yang Zong-cai Feng School of Chemistry and Chemical Engineering Lingnan Normal University Research Center of Chemistry & Materials Lingnan Normal University Development Center for New Materials Engineering & Technology in Universities of Guangdong Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied Chemistry Chinese Academy of Science

For the purpose of increasing the in vivo stability of polycation gene carriers, we prepared a kind of p H-sensitive poly（ethylene glycol）-poly（γ-benzyl-L-glutamate-co-glutamic acid）（PEG-PGA（65）, 65 denotes the molar ratio of glutamic acid in poly（γ-benzyl-L-glutamate-co-glutamic acid））. PEG-PGA（65） showed low cytotoxicity and could shield the positive charge of DNA/PEI（1：1） polyplexes efficiently. The transfection was enhanced due to the partially charge shielding in He La cell line at pH of 7.4. There was almost no transfection efficiency when the surface charge of the ternary particles turned to negative at p H of 7.4. However, the transfection efficiency recovered a lot by culturing at p H of 6.0 at the beginning of transfection. Confocal microscopic observation and flow cytometry results showed DNA/PEI polyplexes should be efficiently released and endocytosized at p H 6.0, because of the p H triggered deshielding action of PEG-PGA（65）. Due to the good biocompatibility and suitable p H triggered shielding/deshielding property, PEG-PGA（65） could be a potential shielding system for polycationic gene carriers used in vivo.

关键词： Gene delivery Shielding system PEGylation pH Sensitive

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Fatty liver diseases, mechanisms, and potential therapeutic plant medicines

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Chinese Journal of Natural Medicines 2020年第3期18卷 161-168页

作者： ZHU Jia-zhen YI hong-Wei HUANG Wei PANG Tao ZHOU Hui-Ping WU Xu-Dong State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing 210023China Department of Pharmacology School of MedicineSoutheast UniversityNanjing 210009China State Key Laboratory of Natural Medicines China Pharmaceutical UniversityNanjing 210009China Department of Microbiology and Immunology and Mc Guire Veterans Affairs Medical Center Virginia Commonwealth UniversityRichmond 23218USA

The liver is an important metabolic organ and controls lipid, glucose and energy metabolism. Dysruption of hepatic lipid metabolism is often associated with fatty liver diseases, including nonalcoholic fatty liver disease(NAFLD), alcoholic fatty liver diseases(AFLD) and hyperlipidemia. Recent studies have uncovered the contribution of hormones, transcription factors, and inflammatory cytokines to the pathogenesis of dyslipidemia and fatty liver diseases. Moreover, a significant amount of effort has been put to examine the mechanisms underlying the potential therapeutic effects of many natural plant products on fatty liver diseases and metabolic diseases. We review the current understanding of insulin, thyroid hormone and inflammatory cytokines in regulating hepatic lipid metabolism, focusing on several essential transcription regulators, such as Sirtuins(SIRTs), Forkhead box O(FoxO), Sterol-regulatory element-binding proteins(SREBPs). We also discuss a few representative natural products with promising thereapeutic effects on fatty liver disease and dyslipidemia.

关键词： Fatty liver disease Lipid metabolism Hormones Transcription factors Inflammatory cytokines Natural products

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Total laparoscopic liver resection in 78 patients

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World Journal of Gastroenterology 2009年第45期15卷 5727-5731页

作者： Lei Zhang Ya-Jin Chen Chang-zhen Shang hong-Wei Zhang Ze-Jian Huang Department of Hepatobiliary Surgery Sun Yat-sen Memorial Hospital Sun Yat-sen University Guangzhou 510120 Guangdong Province China

AIM: To summarize the clinical experience of laparoscopic hepatectomy at a single center. METHODS: Between November 2003 and March 2009, 78 patients with hepatocellular carcinoma (n = 39), metastatic liver carcinoma (n = 10), and benign liver neoplasms (n = 29) underwent laparoscopic hepatectomy in our unit. A retrospective analysis was done on the clinical outcomes of the 78 ***:The lesions were located in segments—(n=3),□(n=16),□(n=24),□(n=11),□(n=11),□(n=9),and □(n=4).The lesion sizes ranged from0.8 to 15 *** number of lesions was three(n=4),two(n=8)and one(n=66)in the study *** procedures included left hemi-hepatectomy(n=7),left lateral lobectomy(n=14),segmentectomy(n=11),local resection(n=39),and resection ofmetastatic liver lesions during laparoscopic surgeryfor rectal cancer(n=7).Laparoscopic liver resectionwas successful in all patients,with no conversion toopen *** four patients received bloodtransfusion(400-800 mL).There were no perioperativecomplications,such as bleeding and biliary *** function of all patients recovered within 1 wk,and no liver failure ***: Laparoscopic hepatectomy is a safe and feasible operation with minimal surgical trauma. It should be performed by a surgeon with sufficient experience in open hepatic resection and who is proficient in laparoscopy.

关键词：切除术腹腔镜肝癌手术切除临床经验手术创伤转移性肝肿瘤

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Cerebrospinal fluid dynamics in Chiari malformation associated with syringomyelia

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Chinese Medical Journal 2007年第3期120卷 219-223页

作者： LIU Bin WANG zhen-yu XIE Jing-cheng HAN hong-bin PEI Xin-long Department of Neurosurgery Peking University Third HospitalBeijing 100083 China Department of Radiology Peking University Third HospitalBeijing 100083 China

Background About 50%--70% of patients with Chiad malformation I （CMI） presented with syringomyelia （SM）, which is supposed to be related to abnormal cerebrospinal fluid （CSF） flow around the foramen magnum. The aim of this study was to investigate the cerebrospinal fluid dynamics at levels of the aqueduct and upper cervical spine in patients with CMI associated with SM, and to discuss the possible mechanism of formation of SM. Methods From January to Apdl 2004, we examined 10 adult patients with symptomatic CMI associated with SM and 10 healthy volunteers by phase-contrast MRI. CSF flow patterns were evaluated at seven regions of interest （ROI）： the aqueduct and ventral and dorsal subarachnoid spaces of the spine at levels of the cerebellar tonsil, C2-3, and C5-6. The CSF flow waveforms were analyzed by measuring CSF circulation time, durations and maximum velocities of cranial- and caudal-directed flows, and the ratio between the two maximum velocities. Data were analyzed by ttest using SPSS 11.5. Results We found no definite communication between the fourth ventricle and syringomyelia by MRI in the 10 patients. In both the groups, we observed cranial-directed flow of CSF in the early cardiac systolic phase, which changed the direction from cranial to caudal from the middle systolic phase to the early diastolic phase, and then turned back in cranial direction in the late diastolic phase. The CSF flow disappeared at the dorsal ROI at the level of C2-3 in 3 patients and 1 volunteer, and at the level of C5-6 in 6 patients and 3 volunteers. The durations of CSF circulation at all the ROIs were significantly shorter in the patients than those in the healthy volunteers （P=-0.014 at the midbrain aqueduct, P=-0.019 at the inferior margin of the cerebellar tonsil, P=-0.014 at the level of C2-3, and P=-0.022 at the level of C5-6）. No significant difference existed between the two groups in the initial point and duration of the caudal-directed CSF flow during a cardiac cycl

关键词： Arnold-Chiari malformation syringomyelia cerebrospinal fluid

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Comparison of the Kinetics of 1-Hexene Metathesis by Ruthenium Carbene Catalysts

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Chinese Chemical Letters 2006年第7期17卷 988-990页

作者： Chen Xi BAI Wen zhen ZHANG Ren HE Yan hong SUN Xiao Ping CAI Research Institute of Jilin Petrochemical Company Ltd. Petrochina Jilin 132021 State Key Laboratory of Fine Chemicals Dalian University of Technology Dalian 116012

A kinetic study of two ruthenium carbene catalysts, （PCy3）2Cl2Ru=CHPh 1 （Grubbs catalyst） and a new catalyst [1,3-bis（2,6-dimethylphenyl）4,5-dihydroimidazol-2-ylidene]（PPh3）（Cl）2Ru-CHPh 3, were conducted in metathesis of 1-hexene. The kinetic behavior of these catalysts at 40, 50, 60 and 70℃ was compared. Complex 3 has the more active catalytic effect at temperatures 40-70℃.

关键词： Ruthenium carbene metathesis.

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高质量发展的体制基础:高水平社会主义市场经济体制

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求是学刊 2023年第6期50卷 1-10,F0003页

作者：乔榛徐宏鑫黑龙江大学经济与工商管理学院哈尔滨150080

党的二十大报告提出,高质量发展是全面建设社会主义现代化国家的首要任务,着力推动高质量发展首先要构建高水平社会主义市场经济体制。由此,发展与体制的关系在一个更高层次上被要求实现新的契合。高质量发展是体现新发展理念的发展,是... 详细信息

党的二十大报告提出,高质量发展是全面建设社会主义现代化国家的首要任务,着力推动高质量发展首先要构建高水平社会主义市场经济体制。由此,发展与体制的关系在一个更高层次上被要求实现新的契合。高质量发展是体现新发展理念的发展,是创新成为第一动力、协调成为内生特点、绿色成为普遍形态、开放成为必由之路、共享成为根本目的的发展。实现这“五大理念”,必须升级社会主义市场经济体制,充分发挥其内含的新型举国体制支持创新、推动协调、助力绿色、助推开放、促成共享功能,使高水平社会主义市场经济体制成为高质量发展的重要基础。

关键词：高质量发展高水平社会主义市场经济体制新型举国体制

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Structural effects of multiple-bonds on aggregating tendencies of long-chain esters

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Chinese Journal of Chemistry 1991年第5期9卷 448-452页

作者： JIANG Xi-Kui JI Guo-zhen LUO Guang-lin Shanghai Institute of Organic Chemistry 345 Ling-Ling LuShanghai 200032

The kinetics of the hydrolysis of p-nitrophenyl esters of three types of single-chain carbo- xylic acids, namely, saturated, olefinic and acetylenic, was investigated in two aquiorgano binary sol- vent systems, DMSO-H_2O and dioxane (DX)-H_2O. The ester probes used are: octanoie (C8), do- decanoic (C12), stearic (C18), oleic (C18-ol), elaidic (C18-el) and stearolic (C18-st). The order of measured CAgC values is: C18-ol>C18-el>C18>C18-st, whereas the order of decreasing magnitude of the observed hydrolytic rate constants of the monomeric species (km) is: C18 >C18-el>C18-ol> C18-st. These rather unanticipated results are discussed.

关键词： Structural effects of multiple-bonds on aggregating tendencies of long-chain esters

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ERK1/2信号通路基因3'UTR多态性与非小细胞肺癌的相关性

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昆明医科大学学报 2024年第3期45卷 7-17页

作者：洪超向旭东李盈甫曹杨陈雪雅李帅邢安灏林牧马千里中国医学科学院&北京协和医学院医学生物学研究所云南昆明650118 昆明医科大学第三附属医院胸外科云南昆明650118 昆明医科大学第一附属医院干疗科云南昆明650032 云南省保山市第二人民医院检验科云南保山678000

目的探究4个ERK1/2信号通路的基因3'UTR区域的单核苷酸多态性(single nucleotide polymorphism,SNP)位点(MAPK1基因中的rs9340,NRAS基因中的rs14804,KRAS基因中的rs712和rs7973450)与非小细胞肺癌(non-small cell lung cancer,NSCLC)的相关性。方法纳入了478名NSCLC患者及480名健康对照者,利用TaqMan探针法对其进行基因分型检测,并分析上述4个SNP与NSCLC的相关性。结果rs9340位点的等位基因在对照组与非小细胞鳞状细胞癌组(squamous cell carcinoma,SCC)中分布频率的差异具有统计学意义(P=0.009),该结果表明rs9340位点的G等位基因可能是非小细胞肺鳞癌的保护性因素(OR=0.67,95%CI:0.50~0.91)。同时,在<50岁年龄组中,rs9340位点的等位基因在对照组和NSCLC组中的分布频率差异具有统计学意义(P=5.07×10^(-4)),该结果表明rs9340等位基因G可能是NSCLC的保护性因素(OR=0.46,95%CI:0.29~0.72)。结论MAPK1基因SNP位点rs9340可能与NSCLC的发生风险相关。

关键词：非小细胞肺癌 ERK1/2信号通路单核苷酸多态性 3'UTR MAPK1

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Numerical simulation of microstructure evolution and microsegregation of U-Nb alloy during solidification process

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China Foundry 2017年第5期14卷 412-415页

作者： Bin Su Zhi-qiang Han hong-zhang Deng Rong Ma Dong Chen Jun Wu zhen-hong Wang Institute of Materials China Academy of Engineering Physics Key Laboratory for Advanced Materials Processing Technology(Ministry of Education) School of Materials Science and EngineeringTsinghua University

In this work, a cellular automaton model has been developed to simulate the microstructure evolution of U-Nb alloy during the solidification process. The preferential growth orientation, solute redistribution in both liquid and solid, solid/liquid interface solute conservation, interface curvature and the growth anisotropy were considered in the model. The model was applied to simulate the dendrite growth and Nb microsegregation behavior of U-5.5 Nb alloy during solidification, and the predicted results showed a reasonable agreement with the experimental results. The effects of cooling rates on the solidification microstructure and composition distribution of U-5.5 Nb were investigated by using the developed model. The results show that with the increase of the cooling rate, the average grain size decreases and the Nb microsegregation increases.

关键词： U-Nb 合金 microsegregation 微观结构细胞的自动机数字模拟 TP391.99 一

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