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Protein & Cell 2015年第9期6卷 693-697页

作者： hong wang (1) (2) Mingzhu wang (1) Na Yang (1) (2) Rui-Ming Xu (1) (2) 1.National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China 2.University of Chinese Academy of Sciences Beijing 100049 China

The building block of eukaryotic chromatin is the nucleo- some core particle （NCP）, which is consisted of -146 bps of DNA wrapped around an octamer of core histones. A tetra- mer of histone H3 and H4 and two H2A-H2B dimers form the histone octamer （Kornberg, 1974; Luger et al., 1997; Tho- mas and Kornberg, 1975）. During DNA replication, nucleo- some disassembly and reassembly occurs at the replication fork, and histone chaperons CAF-1 and ASF1 are principally responsible for the deposition of histones H3 and H4 onto replicated DNA （Kaufman et al., 1995; Tyler et al., 1999）. ASF1 binds a heterodimeric histone H3-H4 complex through its conserved N-terminal domain （aa 1-155） and impedes the formation of the （H3-H4）2 tetramer both inside the nucleus and in the cytoplasm （English et al., 2005）.

关键词： Histone Chaperone Nucleosome Core Particle Quaternary Complex Replicative Helicase HeLa Cell Nuclear Extract

Friction Stir Welding of Aluminum Alloys

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Journal of Wuhan University of Technology(Materials Science) 2004年第1期19卷 61-64页

作者：傅志红 Zhuzhou Institute of Technology

Friction stir welding(FSW),a new solid-state welding technology invited in the early 1990s,enables us weld aluminum alloys and titanium alloys *** processing of FSW,the microstructure in FSW alloys and the factors inf... 详细信息

关键词： friction stir welding stir-welding head aluminum alloy microstructure

Molecular Tissue Engineering:Concepts,Status and Challenge

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Journal of Wuhan University of Technology(Materials Science) 2002年第3期17卷 30-34页

作者：郭晓东 TongjiMedicalCollege HuazhongUniversityofScienceandTechnology

Tissue engineering has confronted many difficulties mainly as follows:1)How to modulate the adherence,proliferation,and oriented differentiation of seed cells, especially that of stemcells. 2) Massive preparation and sustained controllable delivery of tissue inducing factors or plasmid DNA, such as growth factors, angiogenesis stimulators,and so on. 3) Development of 'intelligent biomimetic materials' as extracellular matrix with a good superficial and structural compatibility as well as biological activity to stimulate predictable, controllable and desirable responses under defined *** biology is currently one of the most exciting fields of research across life sciences,and the advances in it also bring a bright future for tissue engineering to overcome these *** recent years,tissue engineering benefits a lot from molecular *** a comprehensive understanding of the involved ingredients of tissue engineering (cells,tissue inducing factors,genes,biomaterials) and the subtle relationships between them at molecular level can lead to a successful manipulation of reparative processes and a better biological *** tissue engineering,the offspring of the tissue engineering and molecular biology,has gained an increasing importance in recent *** offers the promise of not simply replacing tissue,but improving the *** studies presented in this article put forward this new concept for the first time and provide an insight into the basic principles,status and challenges of this emerging technology.

关键词： tissue engineering stem cell gene therapy biomimetic biomaterials molecular biology molecular tissue engineering growth factor delivery

Establishment and Application of a TaqMan Real-Time Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Rubella Virus RNA

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Acta Biochimica et Biophysica Sinica 2006年第10期38卷 731-736页

作者： Li-hong ZHAO~1* Yu-Yan MA~2 hong wang~1 Shu-Ping ZHAO~3 Wei-Ming ZHAO~1 Hua LI~2 Lei-Yi wang~2 1 Department of Microbiology,School of Medicine,Shandong University,Jinan 250012,China 2 Qilu Hospital,Shandong University,Jinan 250012,China 3 Tai'an central Hospital,Tai'an 271000,China 4 Department of Virology,School of Public Health,Shandong University,Jinan 250012,China Department of Microbiology School of Medicine Shandong UniversityJinan 250012 China Qilu Hospital Shandong UniversityJinan 250012 China Tai'an central Hospital Tai'an 271000 China

The aim of this study was to establish and apply a real-time quantitative reverse transcription-polymerase chain reaction （RT-PCR） for rubella virus （RV） ***,the primer and TaqMan probeconcentrations,as well as reaction temperatures were optimized to establish an efficient real-time quantitativeRT-PCR assay for RV ***,an RV-specific PCR amplicon was made as an external standard toestimate the linearity,amplification efficiency,analytical sensitivity and reproducibility of the real timequantitative ***,the assay was applied to quantify RV RNA in clinical samples for rubella *** RV-specific PCR amplicon was prepared for evaluation of the assay at 503 bp,and its original concentrationwas 2.75×10～9 copies/μ*** real time quantitative assay was shown to have good linearity （R2=0.9920）,highamplification efficiency （E=1.91）,high sensitivity （275 copies/ml）,and high reproducibility （variation coefficientrange,from 1.25% to 3.58%）.Compared with the gold standard,the specificity and sensitivity of the assayin clinical samples was 96.4% and 86.4%,***,the established quantitative RT-PCRmethod is a simple,rapid,less-labored,quantitative,highly specific and sensitive assay for RV RNA.

关键词： rubella virus PCR amplicon real-time fluorescence quantitative RT-PCR

Effects of metoclopramide on gastrointestinal myoelectric activity in rats

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World Journal of Gastroenterology 1997年第3期3卷 41-42页

作者： QIN Xiao Min 1, LI hong Fang 1 and wang Long De 2

AIM To investigate the differences of metoclopramide (MCP) action on myoelectric activity of antrum and small intestine. METHODS Ten healthy male Wistar rats, weighing 250g～350g, were anesthetized with ketamine hydrochloride (100mg/kg, intramuscularly). Four pairs of bipolar stainless steel electrodes 3mm apart were implanted on serosal surface of antrum and 1, 10 and 20cm distal to pylorus respectively. Five to 10 days after operation, the gastrointestinal myoelectric activity of fasted rats after intramuscular injection of 2 5, 6 and 12mg/kg MCP respectively ws recorded with a 8 channel EEG machine, and was quantitatively compared with the myoelectric activity after saline injection. RESULTS In fasted rats, 2 5mg/kg MCP increased the amplitude of spike activity (402 0μV±138 4μV, vs 345 0μV±163 4μV, P 1) of small intestine (1cm distal to pylorus), but did not affect the myoelectric activity of antrum. Six and 12mg/kg MCP increased the amplitude of both the slow wave (332 8μV±200 1μV vs 191 2μV±143 9μV, P 1; 330 0μV±197 1μV vs 191 2μV±143 9μV, P 180 5μV±69 7μV vs 121 8μV±63 3μV, P 174 5μV±71 7μV vs 123 8μV±63 3μV, P 1cm distal to pylorus) only that of the amplitude of spike activity (407 3μV±179 0μV vs 345 0μV±163 4μV, P 145 4μV vs 345 0μV±163 4μV, P 1 7%±26 5% vs 47 4%±22 5%, P 1; 59 1%±17 3% vs 47 4%±22 5%, P 1) was increased and with latent period significantly prolonged (2 5min±0 35min vs 0 77min±0 18min, P 1). CONCLUSION Different mechanisms may be involved in increasing the myoelectric activity of antrum and small intestine after MCP administ

关键词： metoclopramide \ stomach \ intestine myoelectric activity

DNA barcoding of Gaultheria L. in China(Ericaceae:Vaccinioideae)

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Journal of Systematics and Evolution 2011年第5期49卷 411-424页

作者： 1~He REN 1,2~Lu LU 1,2~hong wang 1,2~De-Zhu LI 1(Laboratory of Biodiversity and Biogeography,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China) 2(Plant Germplasm and Genomics Center,Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China) (Laboratory of Biodiversity and Biogeography Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China) (Plant Germplasm and Genomics Center Germplasm Bank of Wild Species Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China)

Four DNA barcoding loci,chloroplast loci rbcL,matK,trnH-psbA,and nuclear locus internal transcribed spacer(ITS),were tested for the accurate discrimination of the Chinese species of Gaultheria by using intraspecific and interspecific pairwise P-distance,Wilcoxon signed rank test,and tree-based *** study included 186 individuals from 89 populations representing 30 *** all individuals,single locus markers showed high levels of sequencing universality but were ineffective for species *** chain reaction amplification and sequencing were successful for all four *** ITS and matK showed significantly higher levels of interspecific species delimitation than rbcL and trnH-psbA.A combination of matK and ITS was the most efficient DNA barcode among all studied regions,however,they do not represent an appropriate candidate barcode for Chinese Gaultheria,by which only 11 out of 30 species can be *** rbcL,matK,and trnH-psbA,which were recently proposed as universal plant barcodes,have a very poor capacity for species separation for Chinese *** barcodes may be reliable tools to identify the evolutionary units of this group,so further studies are needed to develop more efficient DNA barcodes for Gaultheria and other genera with complicated evolutionary histories.

关键词： DNA barcoding Ericaceae Gaultheria species discrimination taxonomy Vaccinioideae.

Similar component analysis

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Progress in Natural Science:Materials International 2006年第12期16卷 1343-1347页

作者： ZHANG hong~1**, wang Xin~1, LI Junwei~2 and CAO Xianguang~1(1. Image Center, Astronautics School, BeiHang University, Beijing 100083, China 2. The 207th Institute of the Second Academy of China Aerospace Science Industry Corporation, Beijing 100854, China) Image Center Astronautics School BeiHang University Beijing 100083 China The 207th Institute of the Second Academy of China Aerospace Science Industry Corporation Beijing 100854 China

A new unsupervised feature extraction method called similar component analysis (SCA) is proposed in this paper. SCA method has a self-aggregation property that the data objects will move towards each other to form clusters through SCA theoretically, which can reveal the inherent pattern of similarity hidden in the dataset. The inputs of SCA are just the pairwise similarities of the dataset, which makes it easier for time series analysis due to the variable length of the time series. Our experimental results on many problems have verified the effectiveness of SCA on some engineering application.

关键词： clustering feature extraction similar component.

Quenched moderate deviations principle for random walk in random environment

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Science China Mathematics 2010年第8期53卷 1947-1956页

作者： hong WenMing 1 & wang HuaMing 1,2 1 Key Laboratory of Mathematics and Complex Systems of Ministry of Education & School of Mathematical Sciences,Beijing Normal University,Beijing 100875,China 2 Department of Basic Courses,Business College,Beijing Union University,Beijing 100025,China Key Laboratory of Mathematics and Complex Systems of Ministry of Education & School of Mathematical Sciences Beijing Normal University Beijing China Department of Basic Courses Business College Beijing Union University Beijing China

We derive a quenched moderate deviations principle for the one-dimensional nearest random walk in random environment,where the environment is assumed to be stationary and *** approach is based on hitting time decompos... 详细信息

关键词： random walk in random environment moderate deviations principle hitting time

Design and Expression of a Synthetic phyC Gene Encoding the Neutral Phytase in Pichia pastoris

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Acta Biochimica et Biophysica Sinica 2006年第11期38卷 803-811页

作者： Li-Kou ZOU~(1,3) hong-Ning wang~(2,1*) Xin PAN~4 Tao XIE~1 Qi WU~5 Zi-Wen XIE~1 Wan-Rong ZHOU~1 1 Laboratory of Veterinary and Biotechnology,Sichuan Agricultural University,Ya'an 625014,China 2 Sichuan University Bioengineering Research Center for Animal Disease Prevention and Control,Chengdu 610065,China 3 Faculty of Environment and Resource of Dujiangyan Campus,Sichuan Agricultural University,Dujiangyan 611830,China 4 College of Earth Science,Chengd University of Technology,Chengdu 610059,China 5 College of Life Science,Sichuan Agricultural University,Ya'an 625014,China Laboratory of Veterinary and Biotechnology Sichuan Agricultural UniversityYa'an 625014 China Faculty of Environment and Resource of Dujiangyan Campus Sichuan Agricultural UniversityDujiangyan 611830 China Sichuan University Bioengineering Research Center for Animal Disease Prevention and Control Chengdu 610065 China College of Earth Science Chengdu University of TechnologyChengdu 610059 China College of Life Science Sichuan Agricultural UniversityYa'an 625014 China

The 1074-bp phyCs gene （optimized phyC gene） encoding neutral phytase was designed andsynthesized according to the methylotrophic yeast Pichia pastoris codon usage bias without altering theprotein *** expression vector,pP9K-phyCs,was linearized and transformed in *** of total extracellular phytase activity was 17.6 U/ml induced in Buffered Methanol-complex Medium（BMMY） and 18.5 U/ml in Wheat Bran Extract Induction （WBEI） medium at the flask scale,respectively,improving over 90 folds compared with the wild-type *** enzyme showed temperature optimumof 70℃ and pH optimum of *** enzyme activity retained 97% of the relative activity afterincubation at 80℃ for 5 *** of the heavy glycosylation the expressed phytase had a molecularsize of approximately 51 *** deglycosylation by endoglycosylase H （EndoH_f）,the enzyme had anapparent molecular size of 42 *** property and thermostability was affected by the glycosylation.

关键词： design expression synthetic phyC gene neutral phytase Pichia pastoris

Storage and Spatial Variation of Phosphorus in Paddy Soils of China

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Pedosphere 2009年第6期19卷 790-798页

作者： LIN Jin-Shi SHI Xue-Zheng LU Xi-Xi YU Dong-Sheng wang hong-Jie ZHAO Yong-Cun SUN Wei-Xia State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 2100(78 (China) Department of Geography National University of Singapore 119260 (Singapore) Graduate University of the Chinese Academy of Sciences Beijing 100049 (China) College of Resources and Environment Fujian Agriculture and Forestry University Fuzhou 350002 (China)

Due to the growing concern about the agricultural phosphorus (P) losses pollution, an in-depth understanding of P in paddy soils of China would be helpful in providing a national perspective of the environmental impact of P cycling and fertility on China's farms. In this study, we evaluated the P storage and the P density of paddy soils in China, characterized the spatial variations of P among the subgroups of paddy soils and soil regions in China, and evaluated the P data using GIS-based analysis, which included a newly compiled 1:1 000 000 digital soil map of China, and using 1 490 soil profiles. The available and total P densities of paddy soils were 6.7 and 698.5 g m -3 , respectively. Overall in China, the total P storage within 1 m of paddy soils was estimated to be 330.2 Tg. The P density of paddy soils varied substantially with subgroups due to the different soil water regimes such as groundwater table and soil drainage. The P availability in paddy soils, especially in surface layer, was higher in high temperature and precipitation areas. Further research is needed to examine more anthropogenic impact factors, such as increasing use of chemical fertilizer.

关键词： paddy soil database paddy soil subgroups phosphorus density phosphorus pool