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Development of a Low-cost Polymerase Chain Reaction-based Method for Studying Differentially Expressed Genes in Developing Rice Leaves

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Journal of Integrative Plant Biology 2009年第6期51卷 614-621页

作者： Yin-Wan Wendy Fung hoi Yee Chow Tik Wan Law Biao Dong hoi shan Kwan Faculty of Science The Chinese University of Hong Kong Shatin Hong Kong SAP China Department of Biology The Chinese University of Hong Kong Shatin Hong Kong SAR China

Gene expression studies are important for revealing gene functions putatively involved in biological processes. We were interested in identifying differentially expressed genes during leaf development in rice. We combined the RNA arbitrarily primed-polymerase chain reaction （***） and dot blot hybridization methods to screen a rice leaf primordium cDNA library. Three developmental stages during vegetative growth were examined. The cDNA clones showing different hybridization patterns were further analyzed and verified. Here we demonstrate that the combination of RAP-PCR and dot blot hybridization could provide an efficient and relatively low-cost cDNA library screening approach to discover genes not previously known to be associated with leaf development in rice, We believe that the findings described here will help to elucidate the molecular mechanism（s） underlying the developmental processes of rice leaf

关键词： cDNA clones development dot blot hybridization primordium RNA arbitrarily primed-polymerase chain reaction.

Molecular Evidence for the Hybrid Origin of Bauhinia blakeana(Caesalpinioideae)

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Journal of Integrative Plant Biology 2008年第1期50卷 111-118页

作者： Chun Yin Mak Ka Shing Cheung Pui Ying Yip hoi shan Kwan Department of Biology The Chinese University of Hong KongShatinHong KongChina

Bauhinia blakeana Dunn is the Hong Kong Special Administrative Region emblem and a popular horticultural species in many Asian countries. It was first described as a new species from Hong Kong almost a century ago. This plant is sterile and has long been considered a hybrid, possibly from two related species, B. purpurea and B. variegata. However, not much evidence based on molecular methods was available to support this hypothesis. In this study, sequences of internal transcribed spacer I （ITS1）, rbcL and atpB-rbcL intergenic spacer for five Bauhinia species and two varieties of one of the species were determined and compared. There were two types of ITS1 sequences in B. blakeana, one indistinguishable from that of B. purpurea and the other one identical to that of B. variegata. This confirmed that B. blakeana was a hybrid of these two species. Chloroplast atpB-rbcL intergenic spacer sequence of B. blakeana was identical to that of B. purpurea, indicating that B. purpurea was the female parent. The hybridization event seemed to occur only recently and was a rare incident. Its occurrence was likely facilitated by interspecific pollen competition. It appeared that human efforts played a crucial role in the preservation and ubiquity of B. blakeana.

关键词： atpB-rbcL intergenic spacer Bauhinia internal transcribed spacer 1 interspecific hybrid interspecific pollen competition.

A review on radiofrequency,microwave and high-intensity focused ultrasound ablations for hepatocellular carcinoma with cirrhosis

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Hepatobiliary Surgery and Nutrition 2021年第2期10卷 193-209页

作者： Tan To Cheung Ka Wing Ma wong hoi She Department of Surgery The University of Hong Kong102 Pok Fu Lam RoadHong KongChina

Importance:Hepatocellular carcinoma(HCC)is usually accompanied by liver cirrhosis,which makes treatment of this disease *** transplantation theoretically provides an ultimate solution to the disease,but the maximal surgical stress and the scarcity of liver graft make this treatment option impossible for some *** an ideal situation,a treatment that is safe and effective should provide a better outcome for patients with the ***:This article aims to give a comprehensive review of various types of loco-ablative treatment for *** Review:Loco-ablative treatment bridges the gap between surgical resection and transarterial *** types of ablative therapy have their unique ability,and evidence-based outcome analysis is the most important key to assisting clinicians to choose the most suitable treatment modality for their ***:Radiofrequency ablation(RFA)has a relatively longer history and more evidence to support its *** ablation(MWA)is gaining momentum because of its shorter ablation time and consistent ablation ***-intensity focused ultrasound(HIFU)ablation is a relatively new technology that provides non-invasive treatment for patients with *** has been carried out at centers of excellence and it is a safe and effective treatment option for selected patients with HCC and liver *** and Relevance:Selective use of different loco-ablative therapies will enhance clinicians’treatment options for treatment of HCC.

关键词： High-intensity focused ultrasound(HIFU) hepatocellular carcinoma(HCC) liver cancer radiofrequency ablation(RFA) microwave ablation(MWA) survival non-invasive treatment ablation cirrhosis complication

Crystallization of amorphous silicon beyond the crystallized polycrystalline silicon region induced by metal nickel

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Chinese Physics B 2017年第1期26卷 353-356页

作者： Dongli Zhang Mingxiang Wang Man wong hoi-Sing Kwok Department of Microelectronics Soochow University Suzhou 215006 China Center for Display Research Department of Electronic and Computer Engineering the Hong Kong University of Science and Technology Kowloon Hong Kong China

Crystallization of amorphous silicon（a-Si） which starts from the middle of the a-Si region separating two adjacent metal-induced crystallization（MIC） polycrystalline silicon（poly-Si） regions is observed. The crystallization is found to be related to the distance between the neighboring nickel-introducing MIC windows. Trace nickel that diffuses from the MIC window into the a-Si matrix during the MIC heat-treatment is experimentally discovered, which is responsible for the crystallization of the a-Si beyond the MIC front. A minimum diffusion coefficient of 1.84×10^-9cm^2/s at 550℃ is estimated for the trace nickel diffusion in a-Si.

关键词： metal-induced crystallization poly-Si nickel diffusion coefficient

Genomic data and ecological niche modeling reveal an unusually slow rate of molecular evolution in the Cretaceous Eupteleaceae

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Science China(Life Sciences) 2024年第4期67卷 803-816页

作者： Kun-Li Xiang Sheng-Dan Wu Lian Lian Wen-Chuang He Dan Peng Huan-Wen Peng Xiao-Ni Zhang Hong-Lei Li Jia-Yu Xue Hong-Yan shan Gui-Xia Xu Yang Liu Zhi-Qiang Wu Wei Wang State Key Laboratory of Plant Diversity and Specialty Crops Institute of BotanyChinese Academy of SciencesBeijing 100093China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyLaboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China China National Botanical Garden Beijing 100093China State Key Laboratory of Grassland Agro-Ecosystems and College of Ecology Lanzhou UniversityLanzhou 730000China University of Chinese Academy of Sciences Beijing 100049China College of Landscape Architecture and Life Science/Institute of Special Plants Chongqing University of Arts and SciencesChongqing 402160China College of Horticulture Academy for Advanced Interdisciplinary StudiesNanjing Agricultural UniversityNanjing 210095China Fairylake Botanical Garden Shenzhen&Chinese Academy of SciencesShenzhen 518004China

Living fossils are evidence of long-term sustained ecological ***,whether living fossils have little molecular changes remains poorly known,particularly in ***,we have introduced a novel method that integrates phylogenomic,comparative genomic,and ecological niche modeling analyses to investigate the rate of molecular evolution of Eupteleaceae,a Cretaceous relict angiosperm family endemic to East *** assembled a high-quality chromosome-level nuclear genome,and the chloroplast and mitochondrial genomes of a member of Eupteleaceae(Euptelea pleiosperma).Our results show that Eupteleaceae is most basal in Ranunculales,the earliest-diverging order in eudicots,and shares an ancient whole-genome duplication event with the other *** document that Eupteleaceae has the slowest rate of molecular changes in the observed *** unusually low rate of molecular evolution of Eupteleaceae across all three independent inherited genomes and genes within each of the three genomes is in association with its conserved genome architecture,ancestral woody habit,and conserved niche *** findings reveal the evolution and adaptation of living fossil plants through large-scale environmental change and also provide new insights into early eudicot diversification.

关键词： conservation genomics eudicots evolutionary rates niche conservatism phylogenomics

A landscape of β-Ga_(2)O_(3) Schottky power diodes

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半导体学报:英文版 2023年第9期44卷 47-56页

作者： Man hoi wong Department of Electronic and Computer Engineering Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina

β-Ga_(2)O_(3) Schottky barrier diodes have undergone rapid progress in research and development for power electronic *** paper reviews state-of-the-art β-Ga_(2)O_(3) rectifier technologies,including advanced diode architectures that have enabled lower reverse leakage current via the reduced-surface-field *** device properties including onresistance,breakdown voltage,rectification ratio,dynamic switching,and nonideal effects are summarized for the different *** results on the high-temperature resilience of β-Ga_(2)O_(3) Schottky diodes,together with the enabling thermal packaging solutions,are also presented.

关键词： β-Ga_(2)O_(3) Schottky diodes power device edge termination nickel oxide

Anaerobic biodegradation of PAHs in mangrove sediment with amendment of NaHCO_3

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Journal of Environmental Sciences 2015年第4期27卷 148-156页

作者： Chun-Hua Li Yuk-shan wong Hong-Yuan Wang Nora Fung-Yee Tam State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental Sciences Department of Biology and Chemistry City University of Hong Kong Department of Biology The Hong Kong University of Science and Technology Key Laboratory of Nonpoint Source Pollution Control Ministry of Agriculture/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural Sciences State Key Laboratory on Marine Pollution City University of Hong Kong

Mangrove sediment is unique in chemical and biological properties. Many of them suffer polycyclic aromatic hydrocarbon（PAH） contamination. However, the study on PAH biological remediation for mangrove sediment is deficient. Enriched PAH-degrading microbial consortium and electron acceptor amendment are considered as two effective measures. Compared to other electron acceptors, the study on CO2, which is used by methanogens, is still seldom. This study investigated the effect of Na HCO3 amendment on the anaerobic biodegradation of four mixed PAHs, namely fluorene（Fl）, phenanthrene（Phe）,fluoranthene（Flua） and pyrene（Pyr）, with or without enriched PAH-degrading microbial consortium in mangrove sediment slurry. The trends of various parameters, including PAH concentrations, microbial population size, electron-transport system activities, electron acceptor and anaerobic gas production were monitored. The results revealed that the inoculation of enriched PAH-degrading consortium had a significant effect with half lives shortened by 7–13 days for 3-ring PAHs and 11–24 days for 4-ring PAHs. While Na HCO3 amendment did not have a significant effect on the biodegradation of PAHs and other parameters, except that CO2 gas in the headspace of experimental flasks was *** of the possible reasons is that mangrove sediment contains high concentrations of other electron acceptors which are easier to be utilized by anaerobic bacteria, the other one is that the anaerobes in mangrove sediment can produce enough CO2 gas even without adding Na HCO3.

关键词： Anaerobic biodegradation Electron acceptor amendment Mangrove sediment Na HCO3amendment Polycyclic aromatic hydrocarbon

Second-harmonic generation in periodic fork-shaped χ^((2)) gratings at oblique incidence

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Chinese Optics Letters 2024年第4期22卷 101-105页

作者：盛艳刘山胡效康陈鑫徐天翔赵如薇 Wieslaw Kroli kowski Laboratory of Infrared Materials and Devices Research Institute of Advanced TechnologiesNingbo UniversityNingbo 315211China Engineering Research Center for Advanced Infrared Photoelectric Materials and Devices of Zhejiang Province Ningbo UniversityNingbo 315211China Department of Quantum Science and Technology Research School of PhysicsAustralian National UniversityCanberra ACT 2601Australia School of Physics and Optoelectronics Engineering Xidian UniversityXi’an 710071China

Three-dimensional(3D) nonlinear photonic crystals have received intensive interest as an ideal platform to study nonlinear wave interactions and explore their applications. Periodic fork-shaped gratings are extremely important in this context because they are capable of generating second-harmonic vortex beams from a fundamental Gaussian wave, which has versatile applications in optical trapping and materials engineering. However, previous studies mainly focused on the normal incidence of the fundamental Gaussian beam, resulting in symmetric emissions of the second-harmonic vortices. Here we present an experimental study on second-harmonic vortex generation in periodic fork-shaped gratings at oblique incidence, in comparison with the case of normal incidence. More quasi-phase-matching resonant wavelengths have been observed at oblique incidence, and the second-harmonic emissions become asymmetric against the incident *** results agree well with theoretic explanations. The oblique incidence of the fundamental wave is also used for the generation of second-harmonic Bessel beams with uniform azimuthal intensity distributions. Our study is important for a deeper understanding of nonlinear interactions in a 3D periodic medium. It also paves the way toward achieving highquality structured beams at new frequencies, which is important for manipulation of the orbital angular momentum of light.

关键词： second-harmonic generation nonlinear photonic crystal periodically poled ferroelectric crystal quasi-phase matching nonlinear wavefront shaping

Changes of cell membrane fluidity for mesenchymal stem cell spheroids on biomaterial surfaces

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World Journal of Stem Cells 2022年第8期14卷 616-632页

作者： Chui-Wei wong Hao-Wei Han shan-hui Hsu National Taiwan University Institute of Polymer Science and EngineeringTaipei 10617TaiwanChina National Health Research Institutes Institute of Cellular and System MedicineMiaoli 350TaiwanChina National Taiwan University Research and Development Center for Medical DevicesTaipei 10617TaiwanChina

BACKGROUND The therapeutic potential of mesenchymal stem cells(MSCs)in the form of threedimensional spheroids has been extensively *** underlying mechanisms for the altered cellular behavior of spheroids have also been *** membrane fluidity is a critically important physical property for the regulation of cell behavior,but it has not been studied for the spheroid-forming cells to *** To explore the association between cell membrane fluidity and the morphological changes of MSC spheroids on the surface of biomaterials to elucidate the role of membrane fluidity during the spheroid-forming process of *** We generated three-dimensional(3D)MSC spheroids on the surface of various culture substrates including chitosan(CS),CS-hyaluronan(CS-HA),and polyvinyl alcohol(PVA)*** cell membrane fluidity and cell morphological change were examined by a time-lapse recording system as well as a highresolution 3D cellular image *** and normal/cancer cells were prestained with fluorescent dyes and co-cultured on the biomaterials to investigate the exchange of cell membrane during the formation of heterogeneous cellular *** We discovered that vesicle-like bubbles randomly appeared on the outer layer of MSC spheroids cultured on different biomaterial *** average diameter of the vesicle-like bubbles of MSC spheroids on CS-HA at 37℃ was approximately 10μm,smaller than that on PVA substrates(approximately 27μm).Based on time-lapse images,these unique bubbles originated from the dynamic movement of the cell membrane during spheroid formation,which indicated an increment of membrane fluidity for MSCs cultured on these ***,the membrane interaction in two different types of cells with similar membrane fluidity may further induce a higher level of membrane translocation during the formation of heterogeneous *** Changes in cell membrane fluidity may be a novel path to elucidate the

关键词： Cell membrane fluidity Cell-cell interaction Cellular spheroids Chitosan Hyaluronan