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食管癌患者使用酮咯酸与吻合口漏的关系

中华医学杂志 2021年第30期101卷 2399-2399页

作者： Corsini EM hofstetter wl MD Anderson Esophageal Cancer Working Group 周天欣(编译) 孙亮(编译) 不详北京大学人民医院麻醉科

非甾体抗炎药(NSAID)是目前加速康复外科(ERAS)施行过程中重要的镇痛药物,最近的证据表明,在接受结直肠手术的患者中,术后酮咯酸的使用与吻合口漏之间存在关联,但这种关联在食管癌切除手术中的研究仍不足。随着非阿片类止痛方案及ERAS... 详细信息

非甾体抗炎药(NSAID)是目前加速康复外科(ERAS)施行过程中重要的镇痛药物,最近的证据表明,在接受结直肠手术的患者中,术后酮咯酸的使用与吻合口漏之间存在关联,但这种关联在食管癌切除手术中的研究仍不足。随着非阿片类止痛方案及ERAS理念越来越受到重视,确定围手术期酮咯酸的使用与吻合口瘘之间的可能关联具有重要的临床意义。该研究回顾性收集了2006—2018年在同一机构接受食管腺癌切除术患者的临床资料,总结吻合口漏的发生情况,同时查询该机构药房记录以了解在手术病例住院期间酮咯酸的使用情况;采用多因素logistic回归分析酮咯酸应用与吻合口漏的关系。

关键词：吻合口漏加速康复外科结直肠手术非甾体抗炎药食管腺癌酮咯酸住院期间食管癌患者

Walking the thin line…ten years later:the dilemma of aboveversus below-ground features to support phylogenies in the Russulaceae(Basidiomycota)

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Fungal Diversity 2018年第2期89卷 267-292页

作者： Bart Buyck Stefan Zoller Valerie hofstetter Museum national d’histoire naturelle Departement Systematique et OriginesCP 39Institut pour la SystematiqueEvolutionBiodiversiteSorbonne UniversiteUMR 7205 CNRS MNHN UPMC EPHE12 Rue Buffon75005 ParisFrance Department of Environmental Systems Science Genetic Diversity CentreETH ZurichCHN E 55Universitatstrasse 168092 ZurichSwitzerland Department of Plant Protection Agroscope ChanginsWadenswil Research StationACWRte de Duiller1260 NyonSwitzerland

For the very first time,morpho-anatomical features of both fruiting bodies as well as below-ground structures have been confronted with a newly produced multigene phylogeny of root symbiotic basidiomycetes using one of the most speciose genera of ectomycorrhizal fungi(Russula,Russulales)as an *** this first of two papers,the authors focus more specifically on below-ground *** five-gene phylogeny divides the genus in five main clades,here interpreted as representing seven subgenera,all significantly *** more conserved than features of fruiting bodies,the anatomy of ectomycorrhiza does not allow for an unambiguous characterization of the main clades resolved by phylogenetic analysis,but the anatomy of ectomycorrhiza performs better to naturally classify the species of this *** of fruiting bodies remain much more adequate for the delimitation of terminal clades and are irreplaceable for morphological species *** taxa mostly nest in ancient lineages,but are also present in some terminal clades of otherwise temperate species *** shift from plectenchymatic to pseudoparenchymatic ECM outer mantle structures happened most likely already in the paleotropics,and is here hypothesized to have facilitated a major diversification of the genus with new hosts in the northern *** data as well as our own observations on below ground structures of several Lactifluus species suggests that this genus shares with Russula the absence of lactifers in ECM mantles and rhizomorphs,contrary to species of Lactarius where lactifers are always *** observations on rhizomorphs of species in Multifurca confirm the presence of vessel-like and ladder-like hyphae,also found in the other agarioid genera of this family,while distinct lactifers are only present in the lactarioid,but not in russuloid members of this genus.

关键词： Ectomycorrhizal anatomy Lactifluus Multifurca Multigene phylogeny Rhizomorphs

Molecular evidence for novel Cantharellus (Cantharellales, Basidiomycota) from tropical African miombo woodland and a key to all tropical African chanterelles

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Fungal Diversity 2013年第1期58卷 281-298页

作者： Bart Buyck Frank Kauff Corinne Cruaud Valérie hofstetter Département Systématique etévolution UMR7205Muséum National d’Histoire NaturelleCP39Rue Buffon 1275005 ParisFrance Fachbereich Biologie Abt.Molekulare PhylogenetikTechnische Universität KaiserslauternPostfach 304967653 KaiserslauternGermany Centre national de séquençage Genoscope2 rue Gaston-CrémieuxCP 570691057 Evry cedexFrance Department of plant protection Agroscope Changins-Wädenswil Research Station ACWRte De Duiller1260 NyonSwitzerland

The authors present a combined morphological and molecular approach of the genus Cantharellus in *** descriptions and detailed illustrations are provided for five new species from the Zambezian savannah woodlands in tropical Africa:***,***,***,*** and ***.A maximum likelihood analysis of tef-1 sequences obtained for 83 collections of Cantharellus that are representative of all major groups in world wide Cantharellus,places a total of 13 African chanterelles,including the five newly described *** recognition of a separate genus Afrocantharellus is *** identification key based on the re-examination of all existing type material is provided for all presently known African Cantharellus.

关键词： Afrocantharellus Biodiversity Guineocongolian rain forest Identification key Phylogeny Tanzania Zambezian woodlands

Sequence data reveal a high diversity of Cantharellus associated with endemic vegetation in Madagascar

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Fungal Diversity 2015年第1期70卷 189-208页

作者： Bart Buyck Frank Kauff Emile Randrianjohany Valérie hofstetter Muséum National d’Histoire Naturelle Dépt.Systématique et Evolution CP39UMR 720575005 ParisFrance Fachbereich Biologie Abt.Molekulare PhylogenetikTechnische Universität KaiserslauternPostfach 304967653 KaiserslauternGermany Centre National de Recherche sur l’Environnement(CNRE) BP 1739Lab.de Microbiologie de l’Environnement(LME)AntananarivoMadagascar Department of plant protection Agroscope Changins-Wädenswil Research Station ACWRte De Duiller1260 NyonSwitzerland

This revision of Cantharellus in Madagascar deals with species that are associated with strictly endemic host trees and *** on morphological differences and molecular sequence data of the tef-1 gene,five new species are proposed(***,***,***,*** and ***),as well as one new subspecies,*** ***,whereas *** and *** *** are epitypified.A key is provided to all Cantharellus that grow with native vegetation in Madagascar.

关键词： Biodiversity Cantharellales Identification key New species Phylogeny Taxonomy Transcription elongation factor 1alpha

The unbearable lightness of sequenced-based identification

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Fungal Diversity 2019年第3期96卷 243-284页

作者： Valerie hofstetter Bart Buyck Guillaume Eyssartier Sylvain Schnee Katia Gindro Departement federal del’economie de la formation et de la recherche DEFRRoutede Duillier 60CP10121260 Nyon 1Switzerland Institut de Systematique EvolutionBiodiversite(ISYEB-UMR 7205)Museum Nationald’Histoire NaturelleSorbonne UniversiteCNRSCP3912 Rue Buffon75005 ParisFrance Perigueux France

Using the basic GenBank local alignment search tool program(BLAST)to identify fungi collected in a recently protected beech forest at Montricher(Switzerland),the number of ITS sequences associated to the wrong taxon name appears to be around 30%,even higher than previously *** results rely on the in-depth re-examination of BLAST results for the most interesting species that were collected,*** records for Switzerland,rare or patrimonial species and problematic species(when BLAST top scores were equally high for different species),all belonging to *** paper dissects for the first time a number of sequence-based identifications,thereby showing in every detail—particularly to the user community of taxonomic information—why sequence-based identification in the context of a fungal inventory can easily go *** first conclusion is that in-depth examination of BLAST results is too time consuming to be considered as a routine approach for future inventories:we spent two months on verification of approx.20 *** from the fact that poor taxon coverage in public depositories remains the principal impediment for successful species identification,it can be deplored that even very recent fungal sequence deposits in GenBank involve an uncomfortably high number of misidentifications or errors with associated *** checking the original publications associated with top score sequences for the few examples that were here re-examined,a positive consequence is that we uncovered over 80 type sequences that were not annotated as types in *** and pitfalls of sequence-based identification are discussed,particularly in the light of undertaking fungal *** are made to avoid or reduce some of the major problems with sequence-based ***,the prospects for a more reliable sequence-based identification of fungi remain quite dim,unless authors are ready to check and update the

关键词： Fungal inventory GenBank Nuclear ribosomal DNA internal transcribed spacers(ITS) Switzerland Type sequence

Coupled solid-fluid FE-analysis of an embankment dam

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Frontiers of Structural and Civil Engineering 2011年第1期5卷 53-62页

作者： Michael PERTL Matthias HOFMANN Guenter hofstetter Insitute of Basic Sciences in Civil Engineering University of InnsbruckInnsbruck A-6020Austria

A coupled solid-fluid FE-model for partially saturated soils,characterized by modeling the soil as a three-phase material consisting of a deformable soil skeleton and the fluid phases water and air,is reviewed *** a constitutive model for the soil skeleton,the well-known Barcelona Basic model(BBM)is employed,which is formulated in terms of net stress and matric *** the BBM,a computationally efficient return mapping algorithm is proposed,which only requires the solution of a scalar nonlinear equation at the integration point *** coupled FE-model is applied to the coupled transient numerical simulation of the water flow and the deformations and stresses in an embankment dam.

关键词： multi-phase model unsaturated soil model Barcelona Basic model(BBM) return mapping algorithm embankment dam

Antibodies as Tailor-Made Chiral Selectors: an Interdisciplinary Approach to Enantiomer Separation and Detection

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色谱 2005年第5期23卷 508-519页

作者： hofstetter Heike hofstetter Oliver Department of Chemistry and Biochemistry Northern Illinois University DeKalb Illinois 60115 USADepartment of Chemistry and Biochemistry Northern Illinois University DeKalb Illinois 60115 USA

It has long been known that the configurational isomers of biologically active compounds, e.g., nutrients, pesticides, and drugs, may exhibit different activities in a chiral environment such as the human body. Although the majority of drugs presently in development are chiral, analytical and preparative methods for the quantitative determination and purification of stereoisomers still lag behind. One reason is that commonly used chiral selectors for the direct resolution of enantiomers are not tailor-made for a specific analyte. The identification of suitable selectors for a particular pair of enantiomers still requires considerable experimentation and is generally demanding with regard to material, time and labor. The rational design of chiral host molecules, therefore, represents a challenge in facilitating enantiomer analysis. In this article, we describe how a combination of techniques ranging from organic synthesis to molecular biology yields antibodies of predetermined specificity and stereoselectivity that can be used as tailor-made chiral selectors for the chromatographic separation of enantiomers and their sensitive detection in immunosensors.

关键词：手性化合物手性选择剂对映体分离检测方法

A multilocus phylogeny for worldwide Cantharellus (Cantharellales, Agaricomycetidae)

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Fungal Diversity 2014年第1期64卷 101-121页

作者： Bart Buyck Frank Kauff Guillaume Eyssartier AndréCouloux Valérie hofstetter Départment systématique etévolution CP39 UMR 7205Muséum national d’histoire naturelle75005 ParisFrance Fachbereich Biologie Abt.Molekulare PhylogenetikTechnische Universität KaiserslauternPostfach 304967653 KaiserslauternGermany 78 boulevard Stalingrad24000 PérigueuxFrance Centre national de séquençage Genoscope2 rue Gaston-CrémieuxCP 570691057 Evry cedexFrance Department of Plant Protection Agroscope Changins-Wädenswil Research Station ACWRte De Duiller1260 NyonSwitzerland

After a short historical overview of past systematic studies on Cantharellus,discussing delimitation and species diversity of the genus as well as previous,morphology-based,infrageneric classifications,this paper presents the first molecularly-based infrageneric classification of this genus using a multigene phylogenetic approach(nucLSU,mitSSU,RPB2 and tef-1)on a dataset that covers approximately halve of the described chanterelles worldwide,including many type *** subgenera are recognized and the recognition of subgenus Afrocantharellus as a separate genus is not *** taxonomic value of individual morphological features is discussed as challenged by this new multigene phylogeny which comprises five new sections,one new subgenus and many emendations for previously recognized infrageneric *** paper discusses the observed discrepancy in biodiversity of Cantharellus when comparing between studies that focus either on below-or above-ground presence.A preliminary biogeographic hypothesis suggests an‘out of Africa’Gondwanan origin as a result of vicariance and subsequent migrations.

关键词： Biogeography Ectomycorrhiza Gondwana mitSSU nucLSU RPB2 tef-1 Systematics

What if esca disease of grapevine were not a fungal disease?

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Fungal Diversity 2012年第3期54卷 51-67页

作者： Valérie hofstetter Bart Buyck Daniel Croll Olivier Viret Arnaud Couloux Katia Gindro Agroscope Changins-Wädenswil(ACW) Plant Protection Field Crops and Grapevine/Viticulture and EnologyCP10121260 Nyon1Switzerland Muséum National d’Histoire Naturelle Dépt.Systématique etévolutionCP 39UMR 720557 rue Cuvier75231 Paris cedex 05France Plant Pathology Institute for Integrative BiologyETH Zurich8092 ZurichSwitzerland Centre national de séquençage Genoscope2 rue Gaston-CrémieuxCP 570691057 Evry cedexFrance

Esca disease,which attacks the wood of grapevine,has become increasingly devastating during the past three decades and represents today a major concern in all wine-producing *** disease is attributed to a group of systematically diverse fungi that are considered to be latent pathogens,however,this has not been conclusively *** study presents the first in-depth comparison between the mycota of healthy and diseased plants taken from the same vineyard to determine which fungi become invasive when foliar symptoms of esca *** unprecedented high fungal diversity,158 species,is here reported exclusively from grapevine wood in a single Swiss vineyard *** identical mycota inhabits wood of healthy and diseased adult plants and presumed esca pathogens were widespread and occurred in similar frequencies in both plant *** esca-associated fungi are not transmitted from adult to nursery plants through the grafting *** the presumed esca-associated fungal pathogens are most likely saprobes decaying already senescent or dead wood resulting from intensive pruning,frost or other mecanical injuries as *** cause of esca disease therefore remains elusive and requires well executive scientific *** results question the assumed pathogenicity of fungi in other diseases of plants or animals where identical mycota are retrieved from both diseased and healthy individuals.

关键词： Fungal community Vitis vinifera Esca disease ITS based fungal identification Fungal pathogens