检索结果-南通市图书馆

Label-free biosensing with singular-phaseenhanced lateral position shift based on atomically thin plasmonic nanomaterials

引用

Light(Science & Applications) 2024年第5期13卷 810-822页

作者： Shaodi Zhu Rodolphe Jaffiol Aurelian Crunteanu Cyrille Vézy Sik-To Chan Wu Yuan ho-pui ho Shuwen Zeng Light Nanomaterials&Nanotechnologies(L2n)CNRS-EMR 7004University of Technology of Troyes10000 TroyesFrance. Department of Biomedical Engineering The Chinese University of Hong KongShatinNew TerritoriesHong KongChina XLIM Research Institute UMR 7252 CNRS/University of Limoges123Avenue Albert ThomasLimogesFrance

Rapid plasmonic biosensing has attracted wide attention in early disease diagnosis and molecular biology ***,it was still challenging for conventional angle-interrogating plasmonic sensors to obtain higher sensitivity without secondary amplifying labels such as plasmonic *** address this issue,we developed a plasmonic biosensor based on the enhanced lateral position shift by phase *** singularity presents as a sudden phase retardation at the dark point of reflection from resonating plasmonic substrate,leading to a giant position shift on reflected ***,for the first time,the atomically thin layer of Ge2Sb2Te5(GST)on silver nanofilm was demonstrated as a novel phase-response-enhancing plasmonic *** GST layer was not only precisely engineered to singularize phase change but also served as a protective layer for active silver *** new configuration has achieved a record-breaking largest position shift of 439.3μm measured in calibration experiments with an ultra-high sensitivity of 1.72×10^(8)nm RIU−1(refractive index unit).The detection limit was determined to be 6.97×10^(−7)RIU with a 0.12μm position ***,a large figure of merit(FOM)of 4.54×10^(11)μm(RIU∙°)^(−1)was evaluated for such position shift interrogation,enabling the labelfree detection of trace amounts of *** targeted biosensing experiments,the optimized sensor has successfully detected small cytokine biomarkers(TNF-αand IL-6)with the lowest concentration of 1×10^(−16)*** two molecules are the key proinflammatory cancer markers in clinical diagnosis,which cannot be directly screened by current clinical *** further validate the selectivity of our sensing systems,we also measured the affinity of integrin binding to arginylglycylaspartic acid(RGD)peptide(a key protein interaction in cell adhesion)with different Mn2+ion concentrations,ranging from 1 nM to 1 mM.

关键词： diagnosis protective silver

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

RSPSSL:A novel high-fidelity Raman spectral preprocessing scheme to enhance biomedical applications and chemical resolution visualization

引用

Light(Science & Applications) 2024年第2期13卷 366-386页

作者： Jiaqi Hu Gina Jinna Chen Chenlong Xue Pei Liang Yanqun Xiang Chuanlun Zhang Xiaokeng Chi Guoying Liu Yanfang Ye Dongyu Cui De Zhang Xiaojun yu hong Dang Wen Zhang Junfan Chen Quan Tang Penglai Guo ho-pui ho Yuchao Li Longqing Cong Perry Ping Shum State Key Laboratory of Optical Fiber and Cable Manufacture Technology Guangdong Key Laboratory of Integrated Optoelectronics IntellisenseDepartment of EEESouthern University of Science and TechnologyShenzhen 518055China College of Optical and Electronic Technology China Jiliang UniversityHangzhou 310018China Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and TherapyGuangzhou 510060China Department of Ocean Science and Engineering Southern University of Science and TechnologyShenzhen 518055China Department of Nephrology Chaozhou People’s HospitalChaozhou 521011China Clinical Research Design Division Sun Yatsen Memorial HospitalGuangzhouGuangdong 510120China School of Automation Northwestern Polytechnical UniversityXi’anShaanxi 710072China Department of Biomedical Engineering The Chinese University of Hong KongHong KongChina Guangdong Provincial Key Laboratory of Nanophotonic Manipulation Institute of NanophotonicsJinan UniversityGuangzhou 511443China

Raman spectroscopy has tremendous potential for material analysis with its molecular fingerprinting capability in many branches of science and *** is also an emerging omics technique for metabolic profiling to shape precision ***,precisely attributing vibration peaks coupled with specific environmental,instrumental,and specimen noise is *** Raman spectral preprocessing to remove statistical bias noise and sample-related errors should provide a powerful tool for valuable information ***,we propose a novel Raman spectral preprocessing scheme based on self-supervised learning(RSPSSL)with high capacity and spectral *** can preprocess arbitrary Raman spectra without further training at a speed of~1900 spectra per second without human *** experimental data preprocessing trial demonstrated its excellent capacity and signal fidelity with an 88%reduction in root mean square error and a 60%reduction in infinite norm(L__(∞))compared to established *** this advantage,it remarkably enhanced various biomedical applications with a 400%accuracy elevation(ΔAUC)in cancer diagnosis,an average 38%(few-shot)and 242%accuracy improvement in paraquat concentration prediction,and unsealed the chemical resolution of biomedical hyperspectral images,especially in the spectral fingerprint *** precisely preprocessed various Raman spectra from different spectroscopy devices,laboratories,and diverse *** scheme will enable biomedical mechanism screening with the label-free volumetric molecular imaging tool on organism and disease metabolomics profiling with a scenario of high throughput,cross-device,various analyte complexity,and diverse applications.

关键词： spectral scheme resolution

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Passively and actively enhanced surface plasmon resonance sensing strategies towards single molecular detection

引用

Nano Research 2022年第9期15卷 8367-8388页

作者： Boliang Jia Jiajie Chen Jie Zhou Youjun Zeng ho-pui ho Yonghong Shao Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronics EngineeringShenzhen UniversityShenzhen 518060China School of Physics&Optoelectronic Engineering Guangdong University of TechnologyGuangzhou 510006China Department of Biomedical Engineering The Chinese University of Hong KongShatin 999077Hong KongChina

Surface plasmonic resonance(SPR)has been a corner stone for approaching single molecular detection due to its highsensitivity capability and simple detection mechanism,and has brought major advancements in biomedicine and life science *** decades,the successful integration of SPR with versatile techniques has been ***,several crucial limitations have hindered this technique for practical applications,such as long detection time and low overall *** review aims to provide a comprehensive summary of existing approaches in enhancing the performance of SPR sensors based on“passive”and“active”***,passive enhancement is discussed from a material aspect,including signal amplification tags and modifications of conventional ***,the focus is on the most popular active enhancement methods including electrokinetic,optical,magnetic,and acoustic manipulations that are summarized with highlights on their advantageous features and ability to concentrate target molecules at the detection ***,prospects and future development directions for developing SPR sensing towards a more practical,single molecular detection technique in the next generation are *** review hopes to inspire researchers’interests in developing SPR-related technology with more innovative and influential ideas.

关键词： surface plasmon resonance single molecular detection plasmonic materials nanotweezers(NTs) dielectrophoresis trap

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Low-temperature optothermal nanotweezers

引用

Nano Research 2023年第5期16卷 7710-7715页

作者： Jianxing Zhou Xiaoqi Dai Yuhang Peng Yili Zhong ho-pui ho Yonghong Shao Bruce Zhi Gao Junle Qu Jiajie Chen State Key Laboratory of Radio Frequency Heterogeneous Integration Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceCollege of Physics and Optoelectronics EngineeringShenzhen UniversityShenzhen 518060China Department of Biomedical Engineering The Chinese University of Hong KongHong Kong 999077China Department of Bioengineering and COMSET Clemson UniversityClemsonSC 29634USA

Optical tweezers that rely on laser irradiation to capture and manipulate nanoparticles have provided powerful tools for biological and biochemistry ***,the existence of optical diffraction-limit and the thermal damage caused by high laser power hinder the wider application of optical tweezers in the biological *** the past decade,the emergence of optothermal tweezers has solved the above problems to a certain extent,while the auxiliary agents used in optothermal tweezers still limit their ***,we report a kind of nanotweezers based on the sign transformation of the thermophoresis coefficient of colloidal particles in low-temperature *** a self-made microfluidic refrigerator to reduce the ambient temperature to around 0℃in the microfluidic cell,we can control a single nanoparticle at lower laser power without adding additional agent solute in the *** novel optical tweezering scheme has provided a new path for the manipulation of inorganic nanoparticles as well as biological particles.

关键词： optothermal tweezers optical manipulation microfluidic device thermophoresis thermo-osmotic flow

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Targeted Sub-Attomole Cancer Biomarker Detection Based on Phase Singularity 2D Nanomaterial-Enhanced Plasmonic Biosensor

引用

Nano-Micro Letters 2021年第6期13卷 284-294页

作者： Yuye Wang Shuwen Zeng Aurelian Crunteanu Zhenming Xie Georges Humbert Libo Ma Yuanyuan Wei Aude Brunel Barbara Bessette Jean-Christophe Orlianges Fabrice Lalloué Oliver GSchmidt Nanfang Yu ho-pui ho Department of Biomedical Engineering The Chinese University of Hong KongShatinNew TerritoriesHong KongPeople’s Republic of China CNRS XLIM Research InstituteUMR 7252University of Limoges123Avenue Albert ThomasLimogesFrance Department of Applied Physics and Applied Mathematics Columbia UniversityNew York CityNYUSA Institute for Integrative Nanosciences IFW DresdenHelmholtzstr.20DresdenGermany Faculty of Medicine University of LimogesEA3842-CAPTuRGEIST2 rue du Dr MarclandLimogesFrance

Detection of small cancer biomarkers with low molecular weight and a low concentration range has always been challenging yet urgent in many clinical applications such as diagnosing early-stage cancer,monitoring treatment and detecting ***,a highly enhanced plasmonic biosensor that can overcome this challenge is developed using atomically thin two-dimensional phase change *** precisely engineering the configuration with atomically thin materials,the phase singularity has been successfully achieved with a significantly enhanced lateral position shift *** on our knowledge,it is the first experimental demonstration of a lateral position signal change>340μm at a sensing interface from all optical *** this enhanced plasmonic effect,the detection limit has been experimentally demonstrated to be 10^(-15) mol L^(−1) for TNF-α cancer marker,which has been found in various human diseases including inflammatory diseases and different kinds of *** as-reported novel integration of atomically thin Ge_(2)Sb_(2)Te_(5) with plasmonic substrate, which results in a phase singularity and thus a giant lateral position shift, enables the detection of cancer markers with low molecular weight at femtomolar level. These results will definitely hold promising potential in biomedical application and clinical diagnostics.

关键词： 2D nanomaterials Cancer marker detection Phase singularity Surface plasmon

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CRISPR-powered optothermal nanotweezers:Diverse bio-nanoparticle manipulation and single nucleotide identification

引用

Light(Science & Applications) 2023年第12期12卷 2644-2654页

作者： Jiajie Chen Zhi Chen Changle Meng Jianxing Zhou Yuhang Peng Xiaoqi Dai Jingfeng Li Yili Zhong Xiaolin Chen Wu Yuan ho-pui ho Bruce Zhi Gao Junle Qu Xueji Zhang Han Zhang Yonghong Shao State Key Laboratory of Radio Frequency Heterogeneous Integration Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceCollege of Physics and Optoelectronics EngineeringShenzhen UniversityShenzhen 518060China Department of Biomedical Engineering The Chinese University of Hong KongShatinHong KongChina Department of Bioengineering and COMSET Clemson UniversityClemsonSC 29634USA School of Biomedical Engineering Shenzhen UniversityShenzhenGuangdong 518060China

Optothermal nanotweezers have emerged as an innovative optical manipulation technique in the past decade,which revolutionized classical optical manipulation by efficiently capturing a broader range of ***,the optothermal temperature field was merely employed for in-situ manipulation of nanoparticles,its potential for identifying bio-nanoparticles remains largely ***,based on the synergistic effect of optothermal manipulation and CRIPSR-based bio-detection,we developed CRISPR-powered optothermal nanotweezers(CRONT).Specifically,by harnessing diffusiophoresis and thermo-osmotic flows near the substrate upon optothermal excitation,we successfully trapped and enriched DNA functionalized gold nanoparticles,CRISPR-associated proteins,as well as DNA ***,we built an optothermal scheme for enhancing CRISPR-based single-nucleotide polymorphism(SNP)detection at single molecule level,while also introducing a novel CRISPR methodology for observing nucleotide ***,this innovative approach has endowed optical tweezers with DNA identification ability in aqueous solution which was unattainable *** its high specificity and feasibility for in-situ bio-nanoparticle manipulation and identification,CRONT will become a universal tool in point-of-care diagnosis,biophotonics,and bio-nanotechnology.

关键词： manipulation thermal identification

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparing 11 early warning scores and three shock indices in early sepsis prediction in the emergency department

引用

World Journal of Emergency Medicine 2024年第4期15卷 273-282页

作者： Rex pui Kin Lam Zonglin Dai Eric ho Yin Lau Carrie Yuen Ting Ip ho Ching Chan Lingyun Zhao Tat ChiTsang Matthew Sik hon Tsui Timothy Hudson Rainer Department of Emergency Medicine School of Clinical MedicineLi Ka Shing Faculty of Medicinethe University of Hong KongHong KongChina School of Public Health Li Ka Shing Faculty of Medicinethe University of Hong KongHong KongChina Accident and Emergency Department Queen Mary HospitalHong KongChina

BACKGROUND:This study aimed to evaluate the discriminatory performance of 11 vital sign-based early warning scores(EWSs)and three shock indices in early sepsis prediction in the emergency department(ED).METhoDS:We performed a retrospective study on consecutive adult patients with an infection over 3 months in a public ED in hong *** primary outcome was sepsis(Sepsis-3 definition)within 48 h of ED *** c-statistics and the DeLong test,we compared 11 EWSs,including the National Early Warning Score 2(NEWS2),Modified Early Warning Score,and Worthing Physiological Scoring System(WPS),etc.,and three shock indices(the shock index[SI],modified shock index[MSI],and diastolic shock index[DSI]),with Systemic Inflammatory Response Syndrome(SIRS)and quick Sequential Organ Failure Assessment(qSOFA)in predicting the primary outcome,intensive care unit admission,and mortality at different time ***:We analyzed 601 patients,of whom 166(27.6%)developed ***2 had the highest point estimate(area under the receiver operating characteristic curve[AUROC]0.75,95%CI 0.70-0.79)and was significantly better than SIRS,qSOFA,other EWSs and shock indices,except WPS,at predicting the primary ***,the pooled sensitivity and specificity of NEWS2≥5 for the prediction of sepsis were 0.45(95%CI 0.37-0.52)and 0.88(95%CI 0.85-0.91),*** discriminatory performance of all EWSs and shock indices declined when used to predict mortality at a more remote time ***:NEWS2 compared favorably with other EWSs and shock indices in early sepsis prediction but its low sensitivity at the usual cut-off point requires further modification for sepsis screening.

关键词： Sepsis Emergency department Clinical prediction rule Early warning score Shock index

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genomics-driven derivatization of the bioactive fungal sesterterpenoid variecolin:Creation of an unnatural analogue with improved anticancer properties

引用

Acta Pharmaceutica Sinica B 2024年第1期14卷 421-432页

作者： Dexiu Yan Jemma Arakelyan Teng Wan Ritvik Raina Tsz Ki Chan Dohyun Ahn Vladimir Kushnarev Tsz Kiu Cheung ho Ching Chan Inseo Choi pui Yi ho Feijun Hu Yujeong Kim Hill Lam Lau Ying Lo Law Chi Seng Leung Chun Yin Tong Kai Kap Wong Wing Lam Yim Nikolay S.Karnaukhov Richard Y.C.Kong Maria V.Babak Yudai Matsuda Department of Chemistry City University of Hong KongHong Kong SARChina iGEM Team“VarieCure” City University of Hong KongHong Kong SARChina Department of Biomedical Engineering City University of Hong KongHong Kong SARChina Moscow Clinical Research Center Named After A.S.Loginov Moscow 111123Russian Federation

A biosynthetic gene cluster for the bioactive fungal sesterterpenoids variecolin(1)and variecolactone(2)was identified in Aspergillus aculeatus ATCC *** production of 1 and 2 was achieved in Aspergillus oryzae by expressing the sesterterpene synthase VrcA and the cytochrome P450 ***,the replacement of VrcB with homologous P450s from other fungal terpenoid pathways yielded three new variecolin analogues(5-7).Analysis of the compounds’anticancer activity in vitro and in vivo revealed that although 5 and 1 had comparable activities,5 was associated with significantly reduced toxic side effects in cancer-bearing mice,indicating its potentially broader therapeutic *** study describes the first tests of variecolin and its analogues in animals and demonstrates the utility of synthetic biology for creating molecules with improved biological activities.

关键词： Animal studies Anticancer properties Biosynthesis Natural products Sesterterpenoids Synthetic biology Terpene synthases Variecolin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Advances in inorganic nanoparticles trapping stiffness measurement:A promising tool for energy and environmental study

引用

能源评论(英文) 2023年第2期2卷 13-31页

作者： Xiaolin Chen Jiajie Chen Jianxing Zhou Xiaoqi Dai Yuhang Peng Yili Zhong ho-pui ho Bruce Zhi Gao Han Zhang Junle Qu Yonghong Shao State Key Laboratory of Radio Frequency Heterogeneous Integration Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceCollege of Physics and Optoelectronics EngineeringShenzhen UniversityShenzhen518060China Department of Biomedical Engineering The Chinese University of Hong KongShatinHong Kong Department of Bioengineering and COMSET Clemson UniversityClemsonSC29634USA

Optical tweezers system has emerged as an efficient tool to manipulate tiny particles in a non-invasive *** stiffness,as an essential parameter of an optical potential well,represents the trapping ***,trapping inorganic nanoparticles such as metallic nanoparticles or other functionalized inorganic nanoparticles is important due to their properties of good stability,high conductivity,tolerable toxicity,etc.,which makes it an ideal detection strategy for bio-sensing,force calculation,and determination of particle and environmental ***,the trapping stiffness measurement(TSM)methods of inorganic nanoparticles have rarely been analyzed and ***,in this review,the principle and methods of TSM are *** also systematically summarize the progress in acquiring inorganic particles trapping stiffness and its promising *** addition,we provide prospects of the energy and environment applications of optical tweezering technique and ***,the challenges and future directions of achieving the nanoparticles trapping stiffness are discussed.

关键词： Optical tweezers Optothermal tweezers Trapping stiffness measurement Inorganic nanoparticles Energy and environment applications

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioengineered miR-27b-3p and miR-328-3p modulate drug metabolism and disposition via the regulation of target ADME gene expression

引用

Acta Pharmaceutica Sinica B 2019年第3期9卷 639-647页

作者： Xin Li Ye Tian Mei-Juan Tu pui Yan ho Neelu Batra Ai-Ming Yu Key Laboratory of Molecular Target&Clinical Pharmacology School of Pharmaceutical Sciences&the Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou511436China Lab for Bone Metabolism Key Lab for Space Biosciences and BiotechnologySchool of Life SciencesNorthwestern PolytechnicalUniversityXi'an710072China Department of Biochemistry&Molecular Medicine UC Davis School of MedicineSacramentoCA95817USA

Drug-metabolizing enzymes, transporters, and nuclear receptors are essential for the absorption, distribution, metabolism, and excretion(ADME) of drugs and xenobiotics. MicroRNAs participate in the regulation of ADME gene expression via imperfect complementary Watson–Crick base pairings with target transcripts. We have previously reported that Cytochrome P450 3A4(CYP3A4) and ATP-binding cassette sub-family G member 2(ABCG2) are regulated by miR-27b-3p and miR-328-3p,respectively. Here we employed our newly established RNA bioengineering technology to produce bioengineered RNA agents(BERA), namely BERA/miR-27b-3p and BERA/miR-328-3p, via fermentation. When introduced into human cells, BERA/miR-27b-3p and BERA/miR-328-3p were selectively processed to target miRNAs and thus knock down CYP3A4 and ABCG2 mRNA and their protein levels,respectively, as compared to cells treated with vehicle or control RNA. Consequently, BERA/miR-27b-3p led to a lower midazolam 10-hydroxylase activity, indicating the reduction of CYP3A4 activity. Likewise,BERA/miR-328-3p treatment elevated the intracellular accumulation of anticancer drug mitoxantrone, a classic substrate of ABCG2, hence sensitized the cells to chemotherapy. The results indicate that biologic miRNA agents made by RNA biotechnology may be applied to research on miRNA functions in the regulation of drug metabolism and disposition that could provide insights into the development of more effective therapies.

关键词： Bioengineered RNA miR-27b miR-328 CYP3A4 ABCG2 Drug disposition

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：