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Antioxidant, antimicrobial and cytotoxic potential of condensed tannins from Leucaena leucocephala hybrid-Rendang

Food Science and Human Wellness 2016年第2期5卷 65-75页

作者： Mazni Abu Zarin ho yin wan Azizul Isha Nurdin Armania Laboratory of Vaccines and Immunotherapeutics Institute of BioscienceUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Laboratory of Natural Products Institute of BioscienceUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Department of Biomedical Science Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia

Condensed tannins(CTs)are one of the promising compounds due to their potentially health-promoting *** this study,CTs were extracted from a Leucaena leucocephala hybrid-Rendang and subjected to various biological studies including antioxidant(using Ferric reducing antioxidant power(FRAP),DPPH and ABTS radical scavenging assay),anti-microbial(against different pathogens)and cytotoxic activities(toward human breast adenocarcinoma(MCF-7),human colon carcinoma(HT29),human cervical carcinoma(HeLa)and human liver carcinoma(HepG2)cell lines)in cancer cells through in vitro *** structural characteristics and purity of CTs extract were determined using 13C *** results showed that CTs exhibited higher in vitro antioxidant activities(2257.12±80.55 mg TEAC/g extract,605.3±1.82 mg TEAC/g extract and 1014.03±1.20 mg TEAC/g extract in FRAP,ABTS and DPPH assay,respectively)and demonstrated anti-microbial activities toward selected Gram’s positive and Gram’s negative bacteria tested with MIC and MBC value at 6.25–50 mg/***,among other selected cancer cells,CTs also demonstrated cytotoxic activity toward human breast cancer cells(MCF-7)(IC50=38.33±2.08mg/mL).Characteristic of apoptosis such as cell shrinkage,nuclear condensation and apoptotic bodies were shown in *** preliminary investigations have provided scientific rationale to use CTs as an alternative therapy for various oxidative and inflammatory associated diseases.©2016 Beijing Academy of Food *** and hosting by Elsevier *** rights reserved.

关键词： Antioxidant Anti-microbial Condensed tannins Cytotoxic Leucaena leucocephala

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Cloning of a novel phytase from an anaerobic rumen bacterium, Mitsuokella jalaludinii, and its expression in Escherichia coli

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Journal of Integrative Agriculture 2015年第9期14卷 1816-1826页

作者： TAN wan-qin Phang Chiun Yee Sieo Chin Chin Yiap Beow Chin Clemente Michael Wong Vui Ling Norhani Abdullah Son Radu ho yin wan Laboratory of Vaccines and Immunotherapeutics Institute of BioscienceUniversiti Putra Malaysia Department of Microbiology Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra Malaysia Department of Life Sciences School of Pharmacy and HealthFaculty of Medicine and HealthInternational Medical University Biotechnology Research Institute Universiti Malaysia Sabah Institute of Tropical Agriculture Universiti Putra Malaysia Department of Biochemistry Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra Malaysia Department of Food Science Faculty of Food Science and TechnologyUniversiti Putra Malaysia

The ful length phytase gene of Mitsuokel a jalaludini was successful y cloned and was found to be 1 047 bp in length, with 348 amino acids, and was designated as PHY7 phytase gene. A comparison of the sequence of PHY7 phytase gene of M. jalaludini with various microbial phytase gene sequences showed that it was not similar to those from other bacteria except Selenomonas ruminatium, thus suggesting that they may both express a new class of phytase. The PHY7 phytase gene was subsequently subcloned into bacterial expression vector, pET32a, for expression in Escherichia coli strain Ro-setta-gami. Expression of the recombinant phytase gene was optimised and characterised. The recombinant phytase was estimated to be approximately 55 kDa by SDS-PAGE analysis. The recombinant phytase exhibited optimum activity at 55°C, pH 4.5 and showed good pH stability from pH 3.5 to 5.5 (>78%relative activity). Metal ions such as Ca2+, Mg2+, and K+were found to exert signiifcant stimulatory effect on the recombinant phytase activity while Cu2+, Fe3+, and Zn2+greatly inhibited the enzyme activity. The recombinant phytase showed moderate resistance to trypsin proteolysis, but susceptible to pepsin proteolysis. The results of the study showed that several characteristics of recombinant phytase were slightly different from the native enzyme. Unfavourable characteristics such as reduced pH stability and metal ion effects should be taken into consideration during feed enzyme formulation.

关键词： phytase cloning recombinant Escherichia coli Mitsuokella jalaludinii

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Harnessing data using symbolic regression methods for discovering novel paradigms in physics

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Science China(Physics,Mechanics & Astronomy) 2024年第6期67卷 1-11页

作者： Jianyang Guo wan-Jian yin College of Energy Soochow Institute for Energy and Materials Innovations(SIEMIS)Jiangsu Provincial Key Laboratory for Advanced Carbon Materials and Wearable Energy TechnologiesSoochow UniversitySuzhou 215006China Shanghai Qi Zhi Institute Shanghai 200232China Light Industry Institute of Electrochemical Power Sources Soochow UniversitySuzhou 215006China

In recent years,machine-learning methods have profoundly impacted research in the interdisciplinary fields of ***,most machine-learning models lack interpretability,and physicists doubt the credibility of their conclusions because they cannot be combined with prior physical ***,this review focuses on symbolic regression,which is an interpretable machine-learning ***,the relevant concepts of machine learning are introduced in conjunction with ***,we provide an overview of symbolic regression ***,the recent directions for the application of symbolic regression methods in different subfields of physics are outlined,and an overview of the ways in which the applications of symbolic regression have evolved in the realm of physics is *** major aim of this review is to introduce the basic principles of symbolic regression and explain its applications in the field of physics.

关键词： machine learning genetic programming symbolic regression

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Substrate screening for superclean graphene growth using firstprinciples calculations

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Nano Research 2024年第4期17卷 2216-2222页

作者： Zhihao Li Xiucai Sun Xiaoli Sun wan-Jian yin Zhongfan Liu College of Energy Soochow Institute for Energy and Materials InnovationS(SIEMIS)and Jiangsu Provincial Key Laboratory for Advanced Carbon Materials and Wearable Energy TechnologiesSoochow UniversitySuzhou 215006China Graphene Basic Science Research Center Beijing Graphene Institute(BGI)Beijing 100095China Center for Nanochemistry Beijing Science and Engineering Center for NanocarbonsBeijing National Laboratory for Molecular ScienceCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China

Suppressing the formation of amorphous surface carbon and contaminants during the preparation of graphene by chemical vapor deposition remains an ongoing ***,we analyzed how substrate characteristics affect graphene quality by simulating margin extension,the nucleation process,and defect pegging configurations on mono-crystalline oriented metal substrates with the aim of enhancing graphene *** formation energy and nucleation potential,which are indirect substrate–graphene interaction features,were found to appropriately evaluate graphene *** crystallographic orientation of the metal substrate was discovered to be critical for producing superclean graphene.A low graphene defect density and high nucleation rate on the Cu(100)facet guarantee growth of high-quality graphene,especially in terms of suppressing the formation of amorphous *** addition,rapid kink growth and self-healing on the Cu(100)facet facilitate rapid graphene synthesis,which is also promoted by rapid kink splicing and margin self-repair on this *** study provides theoretical insight useful for the synthesis of superclean graphene.

关键词： superclean graphene defects nucleation metal substrates density functional theory calculations

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ReChoreoNet: Repertoire-based Dance Re-choreography with Music-conditioned Temporal and Style Clues

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Machine Intelligence Research 2024年第4期21卷 771-781页

作者： ho yin Au Jie Chen Junkun Jiang Yike Guo Department of Computer Science Hong Kong Baptist UniversityHong Kong 999077China Department of Computer Science and Engineering The Hong Kong University of Science and EngineeringHong Kong 999077China

To generate dance that temporally and aesthetically matches the music is a challenging problem in three ***,the generated motion should be beats-aligned to the local musical ***,the global aesthetic style should be matched between motion and *** third,the generated motion should be diverse and *** address these challenges,we propose ReChoreoNet,which re-choreographs high-quality dance motion for a given piece of music.A data-driven learning strategy is proposed to efficiently correlate the temporal connections between music and motion in a progressively learned cross-modality embedding *** beats-aligned content motion will be subsequently used as autoregressive context and control signal to control a normalizing-flow model,which transfers the style of a prototype motion to the final generated *** addition,we present an aesthetically labelled music-dance repertoire(MDR)for both efficient learning of the cross-modality embedding,and understanding of the aesthetic connections between music and *** demonstrate that our repertoire-based framework is robustly extensible in both content and *** quantitative and qualitative experiments have been carried out to validate the efficiency of our proposed model.

关键词： Generative model cross-modality learning normalizing flow tempo synchronization style transfer.

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Chemical biology investigation of a triple-action,smart-decomposition antimicrobial booster based-combination therapy against“ESKAPE”pathogens

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Science China Chemistry 2024年第9期67卷 3071-3082页

作者： Min wang Huangsheng Pu Yangfan Xu Chenxuan Wu Yuanxin Gu Qingyun Cai Guoxing yin Peng yin Chunhui Zhang Wing-Leung Wong Muyang wan Yugang Bai Xinxin Feng State Key Laboratory of Chem-/Bio-Sensing and Chemometrics Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biologyand School of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China College of Biology Hunan UniversityChangsha 410082China College of Advanced Interdisciplinary Studies&Hunan Provincial Key Laboratory of Novel Nano Optoelectronic Information Materials and Devices National University of Defense TechnologyChangsha 410073China Nanhu Laser Laboratory National University of Defense TechnologyChangsha 410073China Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research(Ministry of Education) College of Chemistry and Chemical EngineeringHunan Normal UniversityChangsha 410081China State Key Laboratory of Chemical Biology and Drug Discovery Department of Applied Biology and Chemical TechnologyThe Hong Kong Polytechnic UniversityHong Kong SAR 999077China Hunan Provincial Key Laboratory of Anti-Resistance Microbial Drugs The Third Hospital of Changsha(The Affiliated Changsha Hospital of Hunan University)Changsha 410015China

The global antibiotic resistance crisis necessitates urgent *** innovative approach involves potentiating antibiotics and non-antibiotic drugs with adjuvants or boosters.A major drawback of these membrane-active boosters is their limited biocompatibility,as they struggle to differentiate between prokaryotic and eukaryotic *** study reports the chemical biology investigation of a dual-action oligoamidine(OA1)booster with a glutathione-triggered decomposition ***1,when combined with other antimicrobial molecules,exhibits a triple-targeting mechanism including cell membrane disruption,DNA targeting,and intracellular enzyme *** multi-targeting mechanism not only enhances the in vitro and in vivo eradication of antibiotic-resistant“ESKAPE”pathogens,but also suppresses the development of bacterial ***,OA1 maintains its activity in bacterial cells by creating an oxidative environment,while it quickly decomposes in mammalian cells due to high glutathione *** mechanistic insights and design principles may provide a feasible approach to develop novel antimicrobial agents and effective anti-resistance combination therapies.

关键词： antimicrobial booster oligoamidine membrane disruption DNA targeting triggered degradation anti-resistance

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Multimodal Machine Learning Guides Low Carbon Aeration Strategies in Urban Wastewater Treatment

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Engineering 2024年第5期36卷 51-62页

作者： hong-Cheng wang Yu-Qi wang Xu wang wan-Xin yin Ting-Chao Yu Chen-Hao Xue Ai-Jie wang State Key Laboratory of Urban Water Resource and Environment School of Civil and Environmental EngineeringHarbin Institute of Technology(Shenzhen)Shenzhen 518055China Key Laboratory of Drinking Water Safety and Distribution Technology of Zhejiang Province College of Civil Engineering and ArchitectureZhejiang UniversityHangzhou 310058China

The potential for reducing greenhouse gas(GHG)emissions and energy consumption in wastewater treatment can be realized through intelligent control,with machine learning(ML)and multimodality emerging as a promising ***,we introduce an ML technique based on multimodal strategies,focusing specifically on intelligent aeration control in wastewater treatment plants(WWTPs).The generalization of the multimodal strategy is demonstrated on eight ML *** results demonstrate that this multimodal strategy significantly enhances model indicators for ML in environmental science and the efficiency of aeration control,exhibiting exceptional performance and *** random forest with visual models achieves the highest accuracy in forecasting aeration quantity in multimodal models,with a mean absolute percentage error of 4.4%and a coefficient of determination of *** testing in a full-scale plant reveals that the multimodal model can reduce operation costs by 19.8%compared to traditional fuzzy control *** potential application of these strategies in critical water science domains is *** foster accessibility and promote widespread adoption,the multimodal ML models are freely available on GitHub,thereby eliminating technical barriers and encouraging the application of artificial intelligence in urban wastewater treatment.

关键词： Wastewater treatment Multimodal machine learning Deep learning Aeration control Interpretable machine learning

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Magnetic Fields Reduce Apoptosis by Suppressing Phase Separation of Tau-441

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Research 2024年第1期2023卷 181-197页

作者： Wen-Juan Lin Wen-Pu Shi wan-Yi Ge Liang-Liang Chen Wei-hong Guo Peng Shang Da-Chuan yin Key Laboratory for Space Bioscience and Biotechnology School of Life SciencesNorthwestern Polytechnical University127 Youyixi RoadXi'an 710072ShaanxiPR China

The biological effects of magnetic fields(MFs)have been a controversial ***,in recent years,there has been increasing evidence that MFs do affect biological ***,the physical mechanism remains ***,we show that MFs(16 T)reduce apoptosis in cell lines by inhibiting liquid–liquid phase separation(LLPS)of Tau-441,suggesting that the MF effect on LLPS may be one of the mechanisms for understanding the“mysterious”magnetobiological *** LLPS of Tau-441 occurred in the cytoplasm after induction with *** phase-separated droplets of Tau-441 recruited hexokinase(HK),resulting in a decrease in the amount of free HK in the *** cells,HK and Bax compete to bind to the voltage-dependent anion channel(VDAC I)on the mitochondrial membrane.A decrease in the number of free HK molecules increased the chance of Bax binding to VDAC I,leading to increased Bax-mediated *** the presence of a static MF,LLPS was marked inhibited and HK recruitment was reduced,resulting in an increased probability of HK binding to VDAC I and a decreased probability of Bax binding to VDAC I,thus reducing Bax-mediated *** findings revealed a new physical mechanism for understanding magnetobiological effects from the perspective of *** addition,these results show the potential applications of physical environments,such as MFs in this study,in the treatment of LLPS-related diseases.

关键词： inhibited Apoptosis finding

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Variation in Bombyx mori immune response against fungal pathogen Beauveria bassiana with variability in cell wallβ-1,3-glucan

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Insect Science 2024年第1期31卷 211-224页

作者： Yulong wang Zhen Liu Xuebing yin Shihong Liu Kai wang Rongjie wan Haoran Chen Xinyang Li Bo Huang Anhui Provincial Key Laboratory of Microbial Pest Control Anhui Agricultural UniversityHefeiChina

Entomopathogenic fungi are protected by a cell wall with dynamic structure for adapting to various environmental conditions.β-1,3-Glucan recognition proteins activate the innate immune system of insects by recognizing surface molecules of ***,the associations between pathogenicity and the different components of entomopathogenic fungal cell walls remain *** Beauveria bassiana strains were selected that have significantly differing virulence against Bombyx *** molecular mechanisms underlying the immune response in *** were investigated using RNA sequencing,which revealed differences in the immune response to different *** strains at 12 h *** assays revealed thatβ-1,3-glucan content had an opposite trend to that of fungal virulence.β-1,3-Glucan injection upregulated BmβGRP4 expression and significantly reduced the virulence of the high-virulence strain but not that of the medium-virulence or low-virulence ***βGRP4 silencing in *** with RNA interference resulted in the opposite virulence pattern,indicating that the virulence of *** was affected by the cell walls’content ofβ-1,3-glucan,which could be recognized by Bmβ***,interference with the gene CnA(calcineurin catalytic A subunit)involved inβ-1,3-glucan synthesis eliminated differences in virulence between *** *** results indicate that strains of a single species of pathogenic fungi that have differing cell wall components are recognized differently by the innate immune system of ***.

关键词： ombyx mori BmβGRP4 innate immunity insect fungal pathogen virulence

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Porously Reduced 2-Dimensional Bi2O2CO3 Petals for Strain-Mediated Electrochemical CO2 Reduction to HCOOH

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Energy & Environmental Materials 2024年第1期7卷 219-225页

作者： Won Seok Cho Dae Myung hong wan Jae Dong Tae Hyung Lee Chul Jong Yoo Donghwa Lee ho Won Jang Jong-Lam Lee Department of Materials Science and Engineering Pohang University of Science and Technology (POSTECH) Department of Materials Science and Engineering Research Institute of Advanced Materials Seoul National University

Here we introduce bismuth-based catalysts for the efficient electrochemical reduction of CO2to formic acid（HCOOH）,which are composed of petalshaped Bi2O2CO3（BOC） that spontaneously formed from Bi thin film in aqueous carbonate solution at room *** the electrochemical reduction process,the BOC petals transform to reduced BOC（R-BOC）consisting of individual BOC and Bi domains.

关键词： bismuth carbon dioxide reduction formic acid heterointerfaces strain

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